3-MCPD의 생식˙발생독성에 관한 연구

곽승준(Seung Jun Kwack),김순선(Soon Sun Kim),최요우(Yo Woo Choi),이규식(Gyu Seek Rhee),손경희(Kyung Hee Sohn),이이다(Rhee Da Lee),채수영(Soo Young Chae),정용현(Yong-Hyun Chung1),유일재(Il Je Yu1),박귀례(Kui Lea Park) 한국독성학회 2004 Toxicological Research Vol.20 No.2

3-Monochloro-1,2-propanediol(3-MCPD) is a toxic compound, often present in different foods containing acid hydrolyzed(AH) protein, like seasonings and savory food products. The purpose of the present study was to investigate the effects of 3-MCPD on male fertility, sperm and testosterone secretion. In vivo male fertility test was performed for observing the adverse effects of 3-MCPD on the function of male reproductive system and pregnancy outcome. 0.01, 0.05, 0.25, 1 and 5 mg/kg b.w. of 3-MCPD was given daily by gavage to groups of 15 adult male SD rats for 4 weeks. At the end of pre-treatment period, males were mated overnight with normal females. Following morning, males demonstrating successful induction of pregnancy were sacrificed on that day to assess sperm parameters and histopathology of reproductive organs. The resulting pregnant females were sacrificed on day 20 of gestation to evaluate pregnancy outcome. As a result, four-week paternal administration with 3-MCPD resulted in adverse effects on male fertility and pregnancy outcome without remarkable histopathological changes in testes and epididymides; sperm motility, copulation index and fertility index were markedly decreased in the treated group and numbers of live fetuses showed steep dose-response curves. Also, spermatogenesis was investigated in this experiment. However, no<br/> effect was observed on production of sperm in testes treated with 3-MCPD for 4 weeks. Hormone assay was performed for observing the effects of 3-MCPD on testosterone and luteinizing hormone (LH) in blood and testes of male SD rats and cultured primary Leydig cell. In result, significant changes of related hormones did not observed by treatment of 3-MCPD. These results indicated that paternal treatment with 3-MCPD induced spermatotoxic effect, which caused an antifertility on male.

산업독성연구에서의 GLP ( Good Laboratory Practice ) System 도입의 필요성

유일재(Il Je Yu),이준연(Jun Yeon Lee) 한국산업보건학회 2002 한국산업보건학회지 Vol.12 No.1

집단생식독성 발생 28년 후 원인물질 2-bromopropane에 대한 IARC 발암성평가

유일재 ( Il Je Yu ) 한국산업보건학회 2023 한국산업보건학회지 Vol.33 No.1

2-Bromopropane, a causative chemical that caused the outbreak of reproductive toxicity 28 years ago, was classified as Group 2A in the recently held IARC monograph 133 meeting. Korean research data were used as supporting data in the carcinogenicity evaluation of 2-bromopropane and other carcinogens. I would like to share my memories with the researchers at the Occupational Safety and Health Research Institute who worked hard to identify the cause.

흰주의 6가 크롬 흡입폭로에 의한 산화적 손상에 관한 연구

맹승희(Seung Hee Maeng),유일재(Il Je Yu),김현영(Hyeon Yeong Kim),임철홍(Cheol Hong Lim),이용묵(Yong Mook Lee),정호근(Ho Keun Chung),정해원(Hai Won Chung) 한국환경성돌연변이발암원학회 2002 한국환경성돌연변이·발암원학회지 Vol.22 No.4

According to the epidemiological studies in chromium workers, hexavalent chromium is associated with the risk of lung cancer. Cellular oxidative damages by reactive oxygen species produced by hexavalent chromium exposure may play an important role in the carcinogenesis process. We investigated the availabilities of malondialdehyde measurement for the assessments of oxidative damages from chromium exposure with an experimental inhalation study in vivo. Lipid peroxidation, one kind of cellular oxidative damage, was measured in blood plasma of the rats which inhaled the hexavalent chromium mist for 1, 2 and 3 weeks. The concentrations<br/> of malondialdehyde (MDA), the metabolite of lipid peroxidation, in all exposed groups were higher than those of controls with dose-dependant manner. The levels of MDA were also correlated with urine chromium levels of the rats. Therefore, MDA as an indicator of lipid peroxidation could be proper biologic marker for the<br/> assessment of the oxidative damage from chromium exposure, which might be involved in carcinogenesis.

나노 독성의 개념 및 나노입자에 대한 위해성 평가의 필요성

맹승희(Seung Hee Maeng),유일재(Il Je Yu) 한국독성학회 2005 Toxicological Research Vol.21 No.2

Human exposure to nano-sized particles (NSP) has increased over the last century with anthropogenic sources, and the rapid development of nanotechnology becomes an another source of such exposure. Information regarding the safety of nanotechnology and its product, nanoparticles, is urgently needed when assuming exposure through inhalation, oral intake, and penetration across skin is ever increasing as growing nanotechnology rapidly. The recent advancement of biokinetic studies with NSP and newer epidemiologic and toxicologic studies with ultrafine particles can be the basis for the nanotoxicology. Some concepts of nanotoxicology can be known from the results of these results. Specific small size of NSP, when inhaled, facilitates deposition by diffusional mechanism in all regions of the respiratory tract and uptake into cells, transcytosis across epithelial and endothelial cells into the blood and lymph circulation to reach target sites. Translocation along axons and dendrites of neuron makes an access to CNS and ganglia. These biokinetics are dependent on NSP surface chemistry. Risk assessments of NSP include appropriate and relevant doses/concentration selections, the increase effects in the organism and the benefits of possible desirable effects. An interdisciplinary team approach is desirable for nanotoxicology research and an appropriate risk assessment.

강원도 지역에 서식하는 무당개구리에서 생물학적 활성을 지닌 Bombesin 유사 Peptide에 관한 연구

박형진,이윤렬,권혁일,김일,유일재,Park, Hyoung-Jin,Lee, Yun-Lyul,Kwon, Hyeok-Yil,Kim, Yil,Yu, Il-Je 생화학분자생물학회 1992 한국생화학회지 Vol.25 No.3

본 실험에서는 강원도 지역에 집단으로 서식하고 있는 한국산 무당개구리(Bombina orientalis) 피부에서 bombesin 유사 peptide를 순수정제하여 생리활성과 amino acid 서열을 측정하고자 하였다. 무당개구리의 피부를 methanol에 담가 bombesin 유사반응물질(BLI)을 추출한 다음 alkaline alumina에 이은 Sephadex G-10 겔 크로마토그래피와 RP C18 preparative HPLC로 부분정제하였다. 부분정제시 여러 종류의 BLI가 출현하므로 본 연구에서는 전체 BLI의 65%를 점유하는 peak(BLI-K1)를 RP C18 및 SP-이온교환 HPLC를 이용하여 순수정제하였다. 피부조직 420 g에서 $890\;{\mu}g$의 BLI-K1를 순수정제하였으며, 이것은 RP C18, 겔 투과 그리고 SP-이온교환 HPLC 모두에서 합성 bombesin(BBS)과 동일한 정체시간을 나타내었다. 생리활성에서도 BLI-K1은 마취한 흰쥐에서 혈장 gastrin 농도와 혜장 단백질, 중탄산이온, amylase 및 chymotrypsin 분비량을 모두 유의하게 증가시켜 합성 BBS와 동일한 활성을 나타내었다. 또한 BLI-K1의 분자량 및 C-terminal의 11개 amino acid 서열이 합성 BBS과 동일하였기 때문에 한국산 무당개구리의 피부에도 유럽산 무당개구리의 피부에 존재하는 것과 동일한 bombesin이 존재하는 것으로 생각된다. The present study was performed to purify a BLI from the skin of frogs, B. orientalis inhabiting Kangwon-Do, Korea, and then determine its physiological activities and amino acid sequence. A substance that shows bombesin-like immunoreactivity (BLI) was partially purified from crude methanol extract of the frog skin by using a column of alkaline alumina and Sephadex G-10. BLI was further purified by using RP C18 preparative HPLC in which BLI was separated into 5 peaks. The major peak (BLI-K1) containing 65% of total BLI was subjected to purify as a single component by using sequential HPLC of SP-ion exchange and RP C18. Content of BLI in each fraction was monitored by RIA for which bombesin antiserum with a high titer and affinity to BBS was raised in a guinea pig. Eventually, $890\;{\mu}g$ of BLI-K1 was homogeneously purified from 420g of the skin of B. orientalis and it was not differentiated from synthetic BBS in RP C18, gel permeation and SP-ion exchange HPLC. In the physiological activities, BLI-K1 was identical to synthetic BBS in stimulation of gastrin release and exocrine pancreatic secretion including volume, protein, bicarbonate, amylase and chymotrypsin output in anesthetized rats. Furthermore, molecular weight and partial amino acid sequence of BLI-K1 was revealed to be identical to synthetic BBS. Therefore, it is concluded from the above results that the skin of B. orientalis contains the same BBS that has been isolated from the skin of European Bombina.

강원도 지역에 서식하는 무당개구리에서 생물학적 활성을 지닌 Bombesin 유사 Peptide 에 관한 연구

박형진,이윤렬,권혁일,김일,유일재,D . R . Marshak ( Hyoung Jin Park,Yun Lyul Lee,Hyeok Yil Kwon,Yil Kim,Il Je Yu,Daniel r . Marshak ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.3

The present study was performed to purify a BLI from the skin of frogs, B. orientalis inhabiting Kangwon-Do, Korea, and then determine its physiological activities and amino acid sequence. A substance that shows bombesin-like immunoreactivity (BLI) was partially purified from crude methanol extract of the frog skin by using a column of alkaline alumina and Sephadex G-10. BLI was further purified by using RP C18 preparative HPLC in which BLI was separated into 5 peaks. The major peak (BLI-K1) containing 65% of total BLI was subjected to purify as a single component by using sequential HPLC of SP-ion exchange and RP C18. Content of BLI in each fraction was monitored by RIA for which bombesin antiserum with a high titer and affinity to BBS was raised in a guinea pig. Eventually, 890 ㎍ of BLI-K1 was homogeneously purified from 420g of the skin of B. orientalis and it was not differentiated from synthetic BBS in RP C18, gel permeation and SP-ion exchange HPLC. In the physiological activities, BLI-K1 was identical to synthetic BBS in stimulation of gastrin release and exocrine pancreatic secretion including volume, protein, bicarbonate, amylase and chymotrypsin output in anesthetized rats. Furthermore, molecular weight and partial amino acid sequence of BLI-K1 was revealed to be identical to synthetic BBS. Therefore, it is concluded from the above results that the skin of B. orientalis contains the same BBS that has been isolated from the skin of European Bombina.

비침습적 측정방법을 이용한 수종 용제가 자극 피부염에 미치는 영향

유재학(Jae Hak Yoo),은희철(Hee Chul Eun),서대헌(Dae Hun Suh),유일재(Il Je Yu) 대한피부과학회 1994 대한피부과학회지 Vol.32 No.6

Background : Solvents play an immense role in the industria sector. Irritant dermatitis which is more common than allergic contact dermatitis can be caused by solvents, Objective : Our purpose was to compare the skin irritancy of several solvents using human and guinea pig skin models. Methods : The skin responses to short contact with etharol, acetone, dimethylsulfoxide (DMSO) and xylene were measured by visual scoring of erythm, transepidermal water loss and laser doppler flowmetry. Results : The results are summarized as follows . 1. Guinea pig and human skin responses to normal saline, ethanol, and acetone were nearly negligible. 2. Guinea pig skin responses to 99.9% DMSO under occlusion for 0.5 min were assessed by visual scoring system, TEWL, and LDF. They are measured 3+0.(0,1+21.70, 45+12.70 at 5 min after removal of 99.9% DMSQ, and 0.83+0.41, 10.5+3.83, 36+4.0, to 120 min after removal. 3. Guinea pig skin responses to 97% xylene under occlusion for 5 nin were assessed by visual scoring system, TEWL and LDF. They are measured 3+0.00, 1. 5.82, 77+11.7 at 5 min after removal of 97% xylene, and 1.83+0.75, 5.5+3.21, 39.17+11.53 at l2 min after removal. 4. Human skin responses to 75% DMSO under occlusion for 1 min were assessed by visual scoring system, TEWL and LDF. They are measured 2,5+0.5, 63+25.8, 51+13.7 at 5 min after removal of 75% DMSO, and 0.17+0.41, 14.67+15.87, 21.17-8. 1 at 120 min after removal. 5. Human skin responses to 97% xylene under occlusion for 12 min were assessed by visual scoring system, TEWL and LDF. They are measured 2.7+0.52+4.22, 76+14.30 at 5 min after removal of 97% xylene, and 0+0, 2.5+0.55, 3.17+0.98, 120 emOVBI. Concbsion : Short contact vrith DMSO and xylene cause visib erythema and an increase in TEWL and cutaneous blood flow. The reaction patterns in hurr an and guinea pig skin models were similar. (Kor J Dermatol 1994: 32(6): 1026-1034)

정자생성 주기법을 이용한 고환독성 평가 필요성과 정량적인 고환독성 평가방법에 대한 고찰

손우찬(Woo-Chan Son),김종춘(Jong-Choon Kim),유일재(Il Je Yu) 한국독성학회 2003 Toxicological Research Vol.19 No.2

Since histopathological examination was known to be the most sensitive evaluation for testicular toxicity, regulatory authorities have been published the guidelines on practical testicular assay approach. Those guidelines specified details of evaluation including fixation, embedding, staining, histological examination and also seminiferous tubular staging methods. However, there have been confusing understanding among toxicologists and even pathologists on staging theory and its application on industrial testicular toxicity. Guidelines did not intend to conduct quantitative assay with staging but recommended the use of knowledge of staging. To count each tubular stage with statistical<br/> analysis is known to be time consuming and labor burdening work but the significance of toxicity has little value. It also has been pointed out that the application of staging theory for longer-term toxicity considered to be lacking of rationale. It could be recommended that qualitative assay with awareness of germ cell loss is more efficient method rather than quantitative counting of each tubular stage. Therefore it would be required that comprehensive understanding of testicular toxicity evaluation and the use of testicular staging method.

나노기술의 환경,보건,안전성 영향에 관한 연구동향 (나노기술 개발동향)

최붕기 ( Boong Kee Choi ),김경호 ( Kyung Ho Kim ),소대섭 ( Dae Sup So ),유일재 ( Il Je Yu ) 한국공업화학회 2007 공업화학전망 Vol.10 No.1