고지방 식이에 따른 어성초 추출물 투여가 혈청지질 및 항산화 효소 활성에 미치는 영향

정차권(Cha-Kwon Chung),함승시(Sung-Shi Ham),이상영(Sang-Young Lee),오덕환(Duck-Hwan Oh),최수영(Soo-Yong Choi),강일준(Il-Jun Kang),남상명(Sang-Myung Nam) 한국식품영양과학회 1999 한국식품영양과학회지 Vol.28 No.1

어성초는 해독 및 이뇨효과가 있으며 백일해, 기관지염, 간염 등의 증상을 완화한다고 알려져 있다. 본 연구는 어성초 에탄올 추출물을 투여하였을 때 혈청내 지질대사와 생체내 항산화 효소의 활성을 조사하였다. 강원도에서 자생하는 어성초 에탄올 추출물을 4주간 SD rat에 경구투여하였고 동시에 실험식이를 6주 동안 공급하였으며 고지방식이로서 10%의 lard와 1% cholesterol 및 0.25%의 sodium cholate를 공급하였다. 정상식이(C) 및 어성초추출물(CE)을 투여한 결과 총 콜레스테롤 함량이 감소되었다. HDL cholesterol은 정상식이군에 비해 증가되었으며 동맥경화 지수는 54%의 감소를 보였다. 또한 HTR은 어성초 추출물 투여군에서 유의적으로 증가하여 HDL cholesterol의 비율이 증가하였다(p<0.01). 고지방식이군(CL)의 콜레스테롤 함량은 정상식이군에 비해 유의적으로 증가하였으며(p<0.01), 동맥경화지수는 4배나 증가되었다. 어성초 추출물을 투여함으로써 동맥경화지수는 통계적으로 유의적인 감소를 나타내었고(p<0.01). HDL-cholesterol 함량은 고지방식이군(CL)에 비해 53% 증가하였다. 고지방식이 및 어성초 추출물투여군(CLE)은 고지방식이군(CL)에 비해 중성지질 함량이 51% 감소하여 통계적 유의성을 나타냈다(p<0.01). 인지질 또한 어성초 추출물 투여시 대조군에 비해 감소하였다(p<0.01). 어성초 추출물(CLE)을 투여함으로써 GOT, GPT 및 ALP활성은 감소하였고 GST활성과 catalase활성은 정상식이군에 비해 증가하였다. 고지방식이 및 어성초 추출물(CLE)을 투여했을 때 GST활성과 catalase활성은 고지방식이군(CL)에 비해 유의적으로 증가하였고 Cu,Zn-SOD는 어성초 추출물군(CLE)에서 감소하였다(p<0.01). Effects of Houttuynia cordata ethanol extracts on lipid metabolism and antioxidant enzymes in Sprague Dawley male rats were investigated. High fat used in the diet mixture included 10% of lard, 1% of cholesterol and 0.25% of sodium cholate. Total serum cholesterol contents of the rats fed Houttuynia cordata extracts were decreased compared to the control. On the other hand, HDL-cholesterol contents were increased along with the decrease of athrogenic index. When high fat diet was fed, total serum cholesterol contents were significantly increased(p<0.01) with the athrogenic index increase of four times of the control. With the administration of Houttuynia cordata extract HDL-cholesterol was increased by 53% in the high fat diet group. Antioxidant enzymes including GST and catalase activities were increased comparing the control. On the otherhand, the extracts lowered phospholipid(p<0.01), GOT, GPT, Cu,Zn-SOD and alkaline phosphatase enzyme activities in the serum which are related to the liver functions. Therefore, the above results suggest that Houttuynia cordata ethanol extracts can help to maintain normal liver functions and help to protect from peroxidative damages caused by excess dietary fat intake.

비화농성 삼출성 복수를 동반하는 질환에서 복막 침생검의 진단적 의의

정종훈,서용태,오덕환,채종구,정춘해,조건국 朝鮮大學校 附設 醫學硏究所 1986 The Medical Journal of Chosun University Vol.11 No.1

Percutaneous needle biopsies of peritoneum with Abrams needle and Cope needle were done un 64 patients with non-purulent exudative ascites, who was admitted to the department of internal medicine, Chosun University hospital, from January 1978 to Deccmber 1983. The diagnostic significance and safety of the needle biopsy was estimated. The results were as follows. 1. Adequate peritoneal tissue for pathological diagnosis was obtained in 53 cases(82.8%) among 64 cases. (Abrams needle 84.8%, Cope needle 80.6%) 2. Definitive diagnosis was obtained in 31 cases among 53 cases (58.6%). (Tuberculosis 23 cases, malignancy 8 cases). 3. As compared final diagnosis with biopsy result, the rate of final diagnosis corresponding with biopsy diagnosis was 58.8% in tuberculosis and 26.3%, in malignancy, but the rate of biopsy diagnosis corresponding with final diagnosis was 100% in both tuberculosis and malignancy. 4. Cases of chronic non-specific inflammation which were diagnosed by biopsy were 13cases(24.5%)among 53 cases, and among these cases, tuberculosis was 69.2% and malignancy 30.8% on final diagnosis and among 53 cases, histologically normal was 9 cases(16.9%) of which 33.3% was tuberculosis and 66.7% was malignancy on final diagnosis. 5. Of the malignant cases on final diagnosis, Positive cytologic examination of ascitic fluid was 16.7% 6. Primary losions of cancerous peritonitis were stomach cancer (38.9%) hepatocellular cancer(22.2%) colon cancer (11.1%) ovarian cancer (11.1%) Pancreatic cancer (5.6%). 7. Complication of percutaneous peritoneal biopsy was local swelling with leakage of ascitic fluid and the accident rate were 36.4,% in Abraham needle and 3.2% in Cope needle.

원발성 간암 164예에 대한 임상적 관찰

김원학,안기완,오덕환,채종구,홍순표,조건국 朝鮮大學校 附設 醫學硏究所 1986 The Medical Journal of Chosun University Vol.11 No.1

This study was carried out in 164 patients of primary hepatocellular carcinoma confirnied by needle or open surgical biopsy. This cases studied involved the patients who admitted to the department of internal medicine, Chosun University HospitaI during the period from Jan. 1976 to May. 1986. The following results were obtained : 1. The average age was 53.3 years with the ago ranged from 28 years old to 81 years old. The peak age incidence was in the 5th decade, amounting to 33.5% of the total. The ratio between male and female was 4.9 : 1. 2. Duration from onset to diagnosis was 6 months in 158 cases. 3. The chief complaints were abdominal pain, indigestion, anorexia, epigastric discomfort, weight loss, fatigability and dyspnea in order of frequency. Physical examination showed hepatomegaly, ascites, jaundice, edema and fever in order of frequency. 4. A hemoglobin level below 10.0gm% was seen in 23.8% of total cases and hemoglobin level above 10.0gm% was seen in 76.2% of total cases. WBC count between 5, 000/㎣ and 10, 000/㎣ was seen in 57.9% of total cases and its count above 10, 000/㎣ was 29.3% of total cases. 5. Urobilinogenuria was positive in 15(14.3%) and bile in urine above (+) in 32(30.5%) of 105 cases. 6. Clonorchis sinensis infestation was found in 9.8% of the cases. 7. Liver function tests showed SGOT elevation in 77.5%, alkaline phesphatase elevation in 64.6%, serum globulin elevation in 55.5%, serum hypoalbuminemia in 54.3% and total bilirubin elevation in 40.7% in order frequency. 8. Among 164 case of primary hepatocellular carcinoma, HBsAg was positive in 83 cases(50.6%) of 164 case. 9. 164 patients with primary hepatocellular carcinoma showed serum AFP concentration in range of 2.0 to 12,800 ng/㎖. Serum AFP concentrations higher than 45ng/㎖ were noticed in 72.6% and higher than 500 ng/㎖ were in 61.6% of patients. 10. Among 164 cases of primary hepatocellular carcinoma, AFP concentrations below 45ng/㎖, a normal ange, were 27.4%, 46 to 500ng/㎖ were 11.0%, 501 to 1000ng/㎖ were 13.4%. 11. In the patients with primary hepatocellular carcinoma, no significant differences in serum AFP concentrations were observed in regard to sex, but incidence of positive AFP, AFP concentrations above 500ng/㎖, was higher in younger patients than in older age. 12. In the 164 patents with primary hepatocellular carcinoma, serum AFP concentrations higher than 500ng/㎖ was noted in 74.7% of serum HBsAg positive patients and in 48.1% of serum HBsAg negative patients, which showed statistically significant differences in serum AFP concentrations in regard to the occurence of HBsAg. 13. The positive serum AFP(AFP>500ng/㎖) was higher in primary hepatocellular carcinoma patients with liver cirrhosis(87.0%) than in those without liver cirrhosis(63.2%). 14. Liver scanning showed cold area in 132(86.8%) of 152 cases, 61.3% in right lobe, 18.2% in left lobe, 167.% in both lobe and 3.8% in porta hepatis. 15. Pathological classification of 164 cases were 140 cases of hepatocellular carcinima, 21 cases of cholangiocarcinoma and 3 cases of mixed carcinoma. 16. 46(70.8%) of 65 cases were associated with liver cirrhosis, 73.9% of which were macronodular. 17. Incidence of remote metastasis was 21.5% of total cases. The most frequent site was in the lung. 18. 26(81.3%) of 32 cases were died within 6 months after biopsy.