한국의 과학기술 어디까지 왔나 - 발효 기술, 아미노산 발효기술 선진국 수준

양지원,Yang, Ji-Won 한국과학기술단체총연합회 2000 과학과 기술 Vol.33 No.10

우리나라의 생명공학기술 수준은 선진국을 100으로 볼 때 65정도로 열세이지만 분야별로 강세를 보여온 발효기술은 90정도로 선진국에 근접해 있으며 특히 아미노산 발효기술은 높게 평가를 받고 있다. 21세기 선도산업으로 꼽고 있는 생물산업의 경쟁력 우위를 점하기 위해선 더 많은 기술적ㆍ경제적인 투자가 절실하다.

Evaluation on the Quality of Fresh, Conventionally Heated and Ohmically Heated Mulberry Fruit Juice

양지원,한대석,이창호,박성진,김영언,Yang, Ji-Won,Han, Dae-Seok,Lee, Chang-Ho,Park, Sung-Jin,Kim, Young-Eon The East Asian Society of Dietary Life 2014 동아시아식생활학회지 Vol.24 No.1

The aim of this study was to establish the superiority of ohmic heating over conventional heating for the sterilization of mulberry juice. Heat treatment of fresh juice significantly reduced the concentration of soluble solids, lowered the pH, and lowered the reducing sugar content (p<0.01). Color measurements showed decreases in the L and a values and increases in the b, H and C values after heat treatment, although the total color differences were smaller after ohmic heating than after conventional heating of fresh juice. The antioxidant capacities, such as reducing power, FRAP, and DPPH, decreased in the order of fresh juice, ohmically heated juice and conventionally heated juice. Furthermore, the anthocyanin, flovonoid, and total antioxidant capacities of the juices significantly decreased in the same order. Sensory evaluations showed no difference between fresh and ohmically heated mulberry fruit juice excluding off-flavor, whereas conventionally heated juice received significantly lower evaluations. The microbial counts were zero in the juice after either heat treatment. Thus, ohmic heat treatment can be effectively used to sterilize fresh mulberry juice to obtain good shelf life with minimal physicochemical, color, antioxidant and sensory deterioration.

나의 주장-98년 설치되는 특허법원에 기술판사제 도입해야

양지원,Yang, Ji-Won 한국과학기술단체총연합회 1994 과학과 기술 Vol.27 No.11

Myelin Water Fraction MRI in a Case of Clinically Probable Amyotrophic Lateral Sclerosis

양지원,이종호,김응엽,신동훈,Yang, Jiwon,Lee, Jongho,Kim, EungYeop,Shin, Dong Hoon The Korean Society of Clinical Neurophysiology 2016 Annals of Clinical Neurophysiology Vol.18 No.1

Amyotrophic lateral sclerosis (ALS) is a progressive motor neuron degenerative disease that clinically manifests both upper and lower motor neuron signs. However, it is unknown where and how the motor neuron degeneration begins, and conflicting hypotheses have been suggested. Recent advanced radiological techniques enable us to look into ALS neuropathology in vivo. Herein, we report a case with upper motor neuron-predominant ALS in whom the results of brain magnetic resonance imaging (MRI) and myelin water fraction MRI suggest axonal degeneration.

국내 시판 누룽지의 물리화학적 특성 및 항산화 성질 연구

양지원,최일숙,Yang, Ji-won,Choi, Il Sook 한국식품조리과학회 2016 한국식품조리과학회지 Vol.32 No.5

Purpose: Nurungji is a traditional Korean food made by yellowish scorched rice. After getting gelatinization of rice, a thin crust of scorched rice will usually be left in the bottom of the traditional cooking pot. In this study, physicochemical characteristics and antioxidant properties of five commercial nurungji products (CNP1, CNP2, CNP3, CNP4, and CNP5) were evaluated. Methods: Physicochemical properties of the five commercial nurungji products were evaluated with AOAC method. The antioxidant activities were assessed using the 1,1-diphenyl-2-picryl hydrazyl (DPPH), 2,2-azinobis-(3-ethyl-benozothia zoline-6-sulfonic acid)(ABTS), ferric reducing antioxidant power (FRAP), and reducing power assays. Results: Water content was the highest in CNP3, followed by CNP1, CNP2, CNP5, and CNP4. Crude ash content of all nurungji was less than 1%. In Hunter color parameter, the significantly highest a value (redness) and b value (yellowness) were measured in the CNP4 product, meanwhile the lowest in CNP3 (p<0.05). The nurungji products of CNP4 and CNP5 had the significantly higher content in total polyphenols and total flavonoids, compared to those of other products. CNP3 and CNP2 had the lowest in total polyphenols and total flavonoids, respectively. CNP4 and CNP5 products showed the significantly higher values in antioxidant activities, whereas CNP3 had the lowest activity. Conclusion: The high value of antioxidant activities in CNP4 and CNP5 might have been affected primarily by the total polyphenols with increasing browning color during the heat treatment.

누에(Bombyx mori) 번데기 및 한약재의 In Vitro 에스트로젠 활성

양지원,최은미,권무길,구성자,Yang Ji-Won,Choi Eun-Mi,Kwon Mu-Gil,Koo Sung-Ja 동아시아식생활학회 2005 동아시아식생활학회지 Vol.15 No.3

In this study we report on the estrogen activity of silkworm pupa and herb extracts in vitro. The estrogenic activity of these resources was investigated by competition binding assays with estrogen receptor $\alpha(ER{\alpha})\;or\;ER{\beta}$, and viability of MCF-7 cells, a human breast cancer cell line. Saturation ligand-binding analysis of $ER{\alpha}\;and\;ER{\beta}$ revealed that all plant extracts competed with estrogen ligand for binding to both ER subtypes with a similar preference and degree and competed stronger with ligand for binding to $ER{\beta}\;than\;to\;ER{\alpha}$. The highest $ER{\alpha}-binding$ sample was silkworm pupa aqueous extract The highest $ER{\beta}-binding$ sample was silkworm pupa oil. These samples were further tested for bioactivity based on their ability to regulate cell growth rate in ER(+) breast cancer cell line, MCF-7 cells. Our studies showed that silkworm pupa, soritae, sesame, yam, pueraria, malt, ginseng, Polygonum multiflorum, and Curcuma longa significantly stimulated the growth of MCF-7 cells (P<0.05). In summary, these results suggested that silkworm pupa and herbs might be useful as potential phytoestrogens.

인공지능 기반 이직 가능성 예측을 위한 데이터 학습 시스템에 관한 연구

양지원(Yang Ji Won),전은경(Jeon Eun-Kyoung),오승훈(Oh, Seung-Hun),이세형(Lee Seihyoung),유홍연(Yu Hong Yeon),손동훈(Son Dong Hoon),임권섭(Lim Kwon-Seob) 한국통신학회 2022 한국통신학회 학술대회논문집 Vol.2022 No.11

두릅순 에탄올 추출물의 인간유래 피부각질형성세포와 피부섬유아세포에서의 자외선에 의한 광노화 억제효과

양지원(Yang, Jiwon),곽충실(Kwak, Chungshil) 한국영양학회 2016 Journal of Nutrition and Health Vol.49 No.6

본 연구에서는 피부의 표피와 진피에 분포하는 HaCaT 세포와 HDF 세포를 이용하여 항산화효과가 우수한 두릅순 추출물의 처리가 UVB에 의한 피부광노화를 억제할 수 있는지 알아보기 위하여 피부 염증반응과 관련한 사이토카인과 피부의 주요 구성 단백질인 collagen에 영향을 미칠 수 있는 MMP-1, type-I procollagen, TRPV-1 등의 단백질 발현에 미치는 영향을 분석하였다. HaCaT에 두릅순 추출물을 24시간 전처리한 경우 UVB (55 mJ/cm2) 노출로 인해 증가한 염증매개인자인 IL-6, IL-8, PGE2를 유의하게 감소시켰다. 또한, 피부 collagen의 정상적인 구조 및 양에 영향을 미치는 단백질들의 발현을 측정한 결과 HaCaT에서는 UVB 조사로 인해 증가한 TRPV-1과 MMP-1 단백질의 발현이 두릅순 에탄올 추출물의 전처리로 모두 감소하였고, HDF에서는 UVB를 조사한 대조군에 비하여 두릅순 추출물 처리가 MMP-1 단백질 발현을 감소시키는 동시에 collagen의 전구체인 type-I procollagen의 발현을 증가시키는 효과를 보였다. 이들 결과들로부터 항산화효과가 우수한 두릅순 70% 에탄올 추출물은 피부세포에서 UVB에 의한 염증반응을 억제시키는 동시에 피부 collagen의 감소를 억제시킴으로써 피부 광노화를 예방할 수 있는 천연 소재로 이용될 수 있다고 본다. Purpose: Solar ultraviolet (UV) radiation causes inflammation and matrix metalloproteinase (MMP) overexpression and extracellular matrix depletion, leading to skin photoaging such as wrinkle formation, dryness, and sagging. Activation of MMP is influenced by various molecules such as reactive oxygen species (ROS), proinflammatory cytokines, and transient receptor potential vanilloid type (TRPV)-1, which are increased in UV-irradiated skin cells. Aralia elata (AE) ethanolic extract was reported to inhibit ROS generation caused by UVB-irradiation in keratinocytes. In this study, we investigated the photoprotective effect of AE ethanolic extract on UVB-irradiated human keratinocytes (HaCaT) and human dermal fibroblasts (HDF). Methods: AE was freeze-dried, extracted in 70% ethanol, and concentrated. Skin cells were treated with AE extract for 24 h and then exposed to UVB (55 mJ/cm²). After 48 h of incubation, proinflammatory cytokines, MMP-1, type-1 procollagen, and TRPV-1 levels were measured by ELISA or Western blotting. Results: Treatment with AE extract (100 μg/mL) significantly inhibited UVB-induced IL-6, IL-8, and PGE2 production in HaCaT by 25.6%, 5.3%, and 70.2%, respectively, and also inhibited elevation of MMP-1 and TRPV-1 caused by UVB irradiation by 20.0% and 41.9%, respectively (p < 0.05). In HDF, AE extract treatment significantly inhibited both elevation of MMP-1 and reduction of type-1 procollagen caused by UVB irradiation (p < 0.05). In addition, type-1 procollagen was elevated by AE extract treatment in normal HDFs (p < 0.05). Conclusion: AE 70% ethanol extract has photoprotective ability via reduction of proinflammatory mediators, TRPV-1 and MMP-1 production, and elevation of collagen synthesis. Our findings suggest that AE extract might be a good natural material to protect against UVB-induced premature skin aging.

해결중심 집단미술치료가 학교부적응 청소년의 학교생활적응에 미치는 효과

양지원(Jee-Won Yang),김형모(Hyung-Mo Kim) 한국콘텐츠학회 2015 한국콘텐츠학회논문지 Vol.15 No.5

본 연구는 해결중심 집단미술치료가 학교부적응 청소년의 학교생활적응에 미치는 효과에 관하여 살펴보기 위하여, 경기도 K시 K중학교에 다니는 학교부적응청소년 30명을 대상으로 김지은(2005)의 학교생활적응척도로 사전검사 후 프로그램 참여를 희망하는 20명의 대상자를 선정하여 10명씩 실험집단과 통제집단으로 무작위 배치하였다. 실험집단에는 2014년 9월15일부터 2014년 12월 22일까지 사전?사후검사를 포함한 총 12회기의 해결중심 집단미술치료를 실시하였다. 집단 간 동질성을 알아보기 위하여 맨 휘트니 U 검정(Mann Whitney U test)을 실시하였으며, 이후 사전?사후 차이를 알아보기 위해 윌콕슨 부호 순위 검정(Wilcoxon’s signed ranks test)을 실시하였다. 연구결과 해결중심 집단미술치료는 학교부적응청소년의 학교생활적응력을 향상시켰으며 효과적인 방법으로 활용될 수 있는 것으로 나타났다. This study is about the effects of the solution-focused group art therapy which was implemented for school maladjusted adolescents. The pre-test was conducted for 30 students who had difficulties to adapt school life in K middle school at K city, Gyeonggi province. 20 out of the 30 students were chosen to the participants for this study and randomly divided into an experimental group and control group, with 10 in each group. The experimental group received a 12 session solution-focused group art therapy including pre- and post-tests. This study found that the solution-focused group art therapy program improved the school life adaptation competency of the participants. In conclusion, the findings suggest that the solution-focused group art therapy can be a good approach to help adolescents to improve their school life adaptation ability.

중·고등학교 태권도 선수들의 완벽주의성향과 자기관리 및 자신감과의 관계

양지원 ( Yang Ji Won ),이세형 ( Lee Sei Hyoung ) 제주대학교 체육진흥센터(구 제주대학교 체육과학연구소) 2019 체육과학연구 Vol.25 No.-

The purpose of this study was analyze the relationship between perfectionism, self - management and self - confidence of Taekwondo athletes and to examine the role of self - management in self - management and self - confidence. For this purpose, 243 Taekwondo players who were registered as athletes in the Korea sports council gathered data through perfectionism test, self - management questionnaire and confidence questionnaire. In order to analyze the collected data, the following main results were obtained through exploratory factor analysis, reliability analysis and correlation analysis using SPSS 18.0 statistical program. First, the correlation analysis between the variables all of the variables except socially oriented perfectionism and interpersonal relationship showed a positive correlation. second, perfectionism In the relationship between self-direction and confidence Between self-management factors partial mediation effect. third, perfectionism social orientation in the relationship between self-confidence training management showed complete mediating effect. fourth, perfectionism social orientation in the relationship between self-confidence Interpersonal management there was no mediation effect. fifth, perfectionism social orientation in the relationship between self-confidence body management showed complete mediating effect. Seventh, perfectionism other oriented in the relationship between self-confidence training management showed complete mediating effect. Eighth, perfectionism other oriented in the relationship between self-confidence interpersonal management partial mediation effect. Ninth, perfectionism other oriented in the relationship between self-confidence mental management partial mediation effect. The tenth, perfectionism other oriented in the relationship between self-confidence body management partial mediation effect. These results suggest that the higher self - directed perfectionism positively affects self - management and self - confidence. Through this study, the positive effect of self - orientation will help to improve self - confidence and performance by self - management.