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3차원 Co-culture 시스템을 통한 BMSC의 NP-like Cell로의 분화
김동화,김수향,허수진,신지원,김영직,박소희,전재우,신정욱,Kim, D.H.,Kim, S.H.,Heo, S.J.,Shin, J.W.,Kim, Y.J.,Park, S.H.,Jun, J.W.,Shin, J.W. 대한의용생체공학회 2008 의공학회지 Vol.29 No.2
The goal of this study is to investigate the effect and potential of three-dimensional Co-culture of BMSCs (bone marrow stromal Cells) and NP (nucleus pulposus) Cells on the differentiation of BMSCs into NP-like Cells. The NP Cells and BMSCs were isolated and cultured from New Zealand White rabbits. The isolated NP Cells and BMSCs were prepared in different alginate beads. Those two types of beads were separated by a track-etched membrane of $3\;{\mu}m$ pore in a 6-well culture plate. No growth factors were used. In addition to these, NP and BMSC were cultured in the beads independently for control. The number of Cells in Co-culturing system was half of those in two control groups. Proliferation and production of glycosaminoglycan (GAG) were evaluated along with histological observation. The GAG production rate(GAG contents/Cell) of Co-cultured BMSCs were much higher than that of BMSCs cultured alone. The total amounts of GAG produced by BMSCs in Co-culturing system were larger than those produced by BMSCs in control group and were comparable with those produced by NP alone even the number of each Cell was half of BMSCs in Co-culturing system. This study showed the potential of differentiation of BMSCs into NP-like Cells through three-dimensional Co-culture system even without any chemical agents.
강지은,김혜원,백다인,신지원,신혜진,심지선,윤도연,이규리,이보라,이혜민,최주연 이화여자대학교 간호과학대학 2014 이화간호학회지 Vol.- No.48
Purpose: The purpose of this study was to investigate the stress and the physical activity according to working in Korean adolescents. Methods: Data were collected from 3 high schools in Seoul and Gyeonggi-do. The total number of four hundred twenty subjects were participated and they completed general questionnaire, International Physical Activity Questionnaire (Korean version) and adolescents stress scale. Data were analyzed by descriptive statistics and t-test using SPSS 21.0 program. Results: The number of employed was 151 (45.2%) and the number of unemployed was 183 (54.8%). Reasons for working were to earn money (44.0%) and to purchase significant product (35.6%). Moderate physical activities of employed students were more than them of unemployed students and there was significant difference (t=3.261, p˂.001). There was no significant difference between employed and unemployed students in the total score of stress. However, there were significant differences on school stress (t=2.854, p=.005) and economical stress (t=3.959, p˂.001) in the subcategory. The stress of employed was higher than the stress of unemployed. Conclusion: As shown in the above result, employed adolescents reported higher levels of both physical activity and stress than unemployed adolescents, thus indicating that employment status of adolescents has a significant impact on physical and mental health. Nursing implications include developing quality care for employed adolescents based on the community health nursing, and providing health education at schools in terms of adolescent employment.