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이규리,Allan E. David,Jian Zhang,신명철,Victor C. Yang 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.54 No.-
Magnetic iron oxide nanoparticles (MIONs) have received much attention due to their unique properties such as ferromagnetic and superparamagnetic characters. These magnetic properties enable the broad use of MIONs in biomedical applications including magnetic resonance imaging (MRI), magnetically guided delivery, and hyperthermal therapy. In particular, magnetic field guided delivery systems have shown promising potential in the development of targeted drug delivery systems for brain tumors. This system facilitates the extravasation and accumulation of MIONs within the brain tumor under external magnetic field. However, the practical use of MIONs is highly limited due to the large physical size of MIONs required for the sufficient retention and accumulation of particles in the brain tumor. This study aims to enhance the accumulation and retention of MIONs in the brain tumor by in situ formation of large clusters of MIONs. To achieve this goal, MIONs with core size of 100 nm were modified with free thiol end groups by conjugating bi-functional poly(ethylene glycol) (NHS-PEG-SH). It is expected that the prepared MIONs-PEG-SH remain stable during the systemic circulation. When the circulating MIONs-PEG-SH are exposed to the external magnetic field applied to the brain tumor, the local concentration of MIONs-PEGSH can be increased and subsequent interactions among MIONs induce a disulfide bond formation. As a result, in situ formation of the large clusters of MIONs allows enhanced accumulation and retention of MIONs in a rat brain tumor model. Moreover, when doxorubicin is loaded onto the MIONs, the biodistribution of doxorubicin at brain tumor site is highly enhanced, suggesting their potential use in theranostic applications.
Multicistronic IVT mRNA for simultaneous expression of multiple fluorescent proteins
이규리,김수영,서윤미,권효경,권영직,이혁진 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.80 No.-
Synthetic mRNA has emerged as a promising gene expression system for more rapid and controllableinduction of target proteins, due to no need of transnuclear localization. It can efficiently generate largeamount of encoded proteins in cells as compared to that of conventional pDNA. In addition, thedevelopment of in vitro transcription (IVT) technique has enabled the facile modulation of syntheticmRNA’s biochemical properties, further strengthening its applications in pharmaceutical sciences andengineering. In this study, we have investigated the IVT mRNA for simultaneous expression of threedistinctivefluorescent (GFP, RFP, and BFP) proteins. To achieve simultaneous expression of the multipleproteins by a single platform, 2A peptides encoding sequences were used in the design of GRB mRNA. Self-cleavages of 2A peptides in the translated fusion proteins were confirmed by western blot analysis. The expression level of the IVT mRNA was optimized by evaluating the use of chemical modification, themethod of poly A tailing, and the capping conditions of IVT process.
The cutting-edge technologies of siRNA delivery and theirapplication in clinical trials
이규리,장보라,이유리,서은영,유지선,이미진,이주영,이혁진 대한약학회 2018 Archives of Pharmacal Research Vol.41 No.9
siRNA therapeutics allows precise regulation of disease specific gene expression to treat various diseases. Although gene silencing approaches using siRNA therapeutics shows some promising results in the treatment of gene-related diseases, the practical applications has been limited by problems such as inefficient in vivo delivery to target cells and nonspecific immune responses after systemic or local administration. To overcome these issues, various in vivo delivery platforms have been introduced. Here we provide an overview for three different platform technologies for the in vivo delivery of therapeutic siRNAs (siRNA–GalNAc conjugate, SAMiRNA technology, and LNP-based delivery method) and their applications in the treatment of various diseases. In addition, a brief introduction to some rare diseases and mechanisms of siRNA therapeutics-mediated treatment is described.
Development of mRNA realeasing microneedle for vaccine delivery
이규리,정한샘,권효경,김민정,이혁진 한국공업화학회 2016 한국공업화학회 연구논문 초록집 Vol.2016 No.1
There have been lots of interests modulating immune system to cure or prevent various disease. As a new class of vaccine, mRNA have great advantages in terms of safety, easy storability, and ability to induce broad immune response. The immune response is stimulated by delivering mRNA encoding target antigen into the APCs (e.g. dendritic cells (DCs), macrophages). Depending on the target antigens, mRNA vaccine system can be utilized either as a prophylactic or therapeutic vaccine for pandemic or cancer, respectively. To deliver mRNA effectively to the APCs, specific delivery system is needed. Among various delivery system, microneedles have great potential because of their painless and easy-to-apply system reaching dermis area having abundance of APCs especially DCs. In this study, we developed mRNA releasing microneedles for efficient vaccine delivery. The mRNA lipid nanoparticles were embedded into the HA (Hyaluronic Acid) microneedles for vaccine delivery.
턱 당김 운동이 무릎 굽힘폄의 근 수행력에 미치는 영향
이규리,김도형,김근조,Lee, Cu-Rie,Kim, Do-Hyung,Kim, Keun-Jo 대한물리치료과학회 2017 대한물리치료과학회지 Vol.24 No.1
Background: The purpose of the present study is to report the effect of muscle performance on knee flexion and extension by chin in exercise. Methods: This study was Participated in 10 healthy subjects. For conducting the chin-in exercise, subjects are lay down with supine position. Using the rounded towel, subjects was performed contraction of longus colli and longus capatis muscle by push the towel. Chin-in exercise was conducted 3 times a day, 4 times a week for 4 weeks. By using the Biodex system4, We measured absolute muscle strength, relative muscle strength, total exercise quantity and average rate of production in knee flexion and extension. The data was analyzed by the repeated-measure ANOVA for comparing before, after exercise 2 weeks, after exercise 4 weeks changes of factors. Results: After chin-in exercise, there was significant difference of before, after 2 weeks and 4weeks results in absolute muscle strength, relative muscle strength, total exercise quantity, average rate of production, agonist/antagonist ratio(p<.05). Conclusion: As a results of this study, chin-in exercise may help to improve muscle ability of knee joint activation and knee joint action performance.
장애인의 생활환경을 위한 유니버설 디자인의 적용에 관한 연구
이규리,김태환,Lee, Cu-Rie,Kim, Tae-Hwan 대한물리치료과학회 1999 대한물리치료과학회지 Vol.6 No.3
In the present day. there has been concern to improve the environment which included with no age limit and disability. But many buildings differentiate people with disabilities from people with non-disabilities and it is problem that infringe upon impartiality. Because people have a variety of age, disability, health-condition and so forth, they do not want to a target of special relation. As an alternative plan, it is necessary to introduce the universal design which is able to use for every body and all-round environment. Based on universal design, this paper reports what can be apply from building service from building and proposes basic of the spatial building service to improve the quality of life of people.