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RISS 인기검색어
- 검색키워드
- 저자 : 선우양일(Yang-Il Sunwoo) (검색결과 32 건)
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Dimethylnitrosamine이 흰쥐의 내분비에 미치는 영향
박기현,선우양일,Park, Ki-Hyun,SunWoo, Yang-Il 생화학분자생물학회 1981 한국생화학회지 Vol.14 No.1
1. 배란기 전에 dimethylnitrosamine (DMN)을 rat에 투여하면 progesterone과 estradiol-$17{\beta}$의 분비를 억제시키나 DMN처리 농도에 따른 hormone 분비양에는 큰 차이가 없었다. 2. 임신한 rat에 DMN을 투여하면 배란기와 착상초기 까지는 estradiol-$17{\beta}$의 분비를 억제시키고 착상기가 진행됨에 따라 분비를 촉진시킨다. 3. 또한 임신한 rat에 DMN을 투여했을 때 progesterone 분비에 미치는 영향은 estradiol-$17{\beta}$의 경우와 유사한 경향을 나타내었다. 1. When dimethylnitrosamine (DMN) was injected to Wistar female rat before the ovulation, the sercetion of progesterone and estradiol-$17{\beta}$ was substantially suppressed. However, the different levels of these hormone secretion according to the DMN concentration was not significant. 2. In the case of the pregnant rats, DMN inhibited the secretion of estradiol-$17{\beta}$ at the stage of ovulation and early implantation. In contrast with the above facts, the secretion of this hormone was enhanced during the progressive implantation. 3. A similar behavior with the one of estradiol-$17{\beta}$ was also observed in the secretion of progesterone for the case of the pregnant rats treated with DMN.
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Saccharomyces cerevisiae의 감수분열 특이적 Protein Kinase인 Ime2의 역할
임선희,탁연수,선우양일,Leem, Sun-Hee,Tak, Yon-Soo,Sunwoo, Yang-Il 한국미생물학회 1999 미생물학회지 Vol.35 No.4
Entry into meiosis in the yeast Saccharomyces cerevisiae is regulated by two major factors: the cell type MATa/MAT${\alpha}$ and the nutriational state (starvation) of the cell. The two independent regulations act through IME1and IME2 expression to initiate meiosis. IME2 encodes a meiosis-specific protein kinase, and it enabled MATa/MAT${\alpha}$ diploid cells to undergo meiosis and sporulation. The PCR mutagenesis method was applied for the isolation of thermosensitive ime2 mutants. Among sixty two mutants isolated from the phenotype of defective spore formation under the restrictive temperature, three with the most easily observed temperature-sensitive phenotype (ts ${\cdot}$ime2-11, ts ${\cdot}$ime2-12 and ts ${\cdot}$ime2-13) were selected for further study. To understand the detailed functions of IME2, we examined the defects of these mutants in the early meiotic pathway including the premeiotic DNA replication and exhibited decreased level in meiotic recombination. These results suggest that the IME2 gene plays essential role in meiotic recombination pathway as well as premeiotic DNA replication. As the result of the IME2 overexpression in ${\Delta}$mre4. moreover, it was suggested that the IME2 and MRE4 genes act on the same pathway of initiation step in meiotic recombination.
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옥수수 분리 엽록체에서의 엽록소 형광의 소멸과 광계 2의 Quantum Yield
이진범,박인호,이혜주,선우양일,Lee, Chin- Bum,Park, In-Ho,Lee, Hae-Joo,Sunwoo, Yang-Il 생화학분자생물학회 1991 한국생화학회지 Vol.24 No.6
옥수수 엽육세포의 분리엽록체를 이용하여 환경요인(광 및 온도)에 따른 산소소비량과 방출되는 형광을 측정하여, 형광소멸 요인을 조사하고 이들과 광계 2의 quantum yield와의 관계를 알아보고자 하였다. 고등식물에 있어 틸라코이드 막은 흡수된 빛의 분포 및 소실을 조절하는 수개의 기작을 가지며, 이들은 내외의 여러 요인들에 의하여 조절되어진다(Horton et al., 1989), 'Light doubling techniques' 와 달리 DCMU처리에 의한 방법은 energy-dependent quenching(qE)을 확실히 구별할 수 있는 장점이 있다. 광의 세기가 낮을 때는 photochemical quenching(qQ)이 형광소멸의 주된 요인임을 알 수 있으며, 광의 세기가 높을수록 non-photochemical quenching(qNP)이 주된 요인이었다. qQ와 산소소비량에 대한 quantum yield$({\Phi}_s)$사이에는 nonlinear한 관계를 보였으며, 이는 높은 광에서 광계 2의 quantum yield(intrinsic yield, $({\Phi}_p)$의 감퇴를 나타낸다 하겠다. ${\Phi}_p$는 광도의 증가에 따라 감퇴하였으며, qE와 역비례 관계를 보였으나 ${\Phi}_p$의 감퇴는 qE의 상승에 기인하기 보다는 pH변화 등 다른 요인의 영향일 것이다. 온도상승에 따라 산소소비량은 증가하였으나, qE, qR 및 qNP는 계속적인 감소경향을 보였다. 한편, $(F_v){_m/F_m}$와 ${\Phi}_p$와의 관계는 변화양상이 매우 유사하여 linear관계를 가질 것으로 사료되며, 이와 같은 관계를 가질 경우, 안테나에서의 열손실이 중요한 요인으로 작용할 것이다. The relationship between quantum yield of photosystem II and quenching component of chlorophyll fluorecene has been investigated through a series of experiments on isolated maize mesophyll chloroplasts. The thylakoid membranes of higher plants possess several mechanisms that control both the distribution and the rate of dissipation of absorbed light. These mechanism allow regulation by external and internal factors. By using DCMU addition techniques, there is an advantage to be able to distinguish energy-dependent quenching (qE) from other components of non-photochemical quenching (qNP). At low light intensity, photochemical quenching (qQ) is major component of chlorophyll fluorescence quenching, while at high light intensity, qNP is major one, The relationship between qQ ans quantum yield of oxygen evolution $({\Phi}_s)$ with incident light intensity was showed. The nonlinear relation is indicative of the decline in the intrinsic yield of photosystem II $({\Phi}_p)$ at high light intensity. ${\Phi}_p$ declined as the light intensity increased, which was inversely proportional to qE. The oxygen evolution was increased gradually as the temperature increases, but over $45^{\circ}C$, decreased rapidly. qE, qR (the remaining quenching) and qNP were continuously decreased, while qQ was rapidly increased over $30^{\circ}C$. The changes of $(F_v){_m/F_m}$ on temperature may be presumably able to suggest that they are affected by the phophorylation of LHC II. And interestingly, the changes of $(F_v){_m/F_m}$ were very similiar with those of ${\Phi}_p$.
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효모의 재조합 변이주를 이용한 인간 Centromeric Alphoid DNA Repeat의 안정성에 관한 연구
김광섭,신영선,이상엽,안은경,도은주,박인호,임선희,선우양일,Kim, Kwang-Sup,Shin, Young-Sun,Lee, Sang-Yeop,Ahn, Eun-Kyung,Do, Eun-Ju,Park, In-Ho,Leem, Sun-Hee,SunWoo, Yang-Il 한국미생물학회 2007 미생물학회지 Vol.43 No.4
The centromere is a highly differentiated structure of the chromosome that fulfills a multitude of essential mitotic and meiotic functions. Alphoid DNA (${\alpha}$-satellite) is the most abundant family of repeated DNA found at the centromere of all human chromosomes, and chromosomes of primates in general. The most important parts in the development of Human Artificial Chromosomes (HACs), are the isolation and maintenance of stability of centromeric region. For isolation of this region, we could use the targeting hook with alphoid DNA repeat and cloned by Transformation-Associated Recombination (TAR) cloning technique in yeast Saccharomyces cerevisiae. The method includes rolling-circle amplification (RCA) of repeats in vitro to 5 kb-length and elongation of the RCA products by homologous recombination in yeast. Four types of $35\;kb{\sim}50\;kb$ of centromeric DNA repeat arrays (2, 4, 5, 6 mer) are used to examine the stability of repeats in homologous recombination mutant strains (rad51, rad52, and rad54). Following the transformation into wild type, rad51 and rad54 mutant strains, there were frequent changes in inserted size. A rad52 mutant strain showed extremely low transformation frequency, but increased stability of centromeric DNA repeat arrays at least 3 times higher than other strains. Based on these results, the incidence of large mutations could be reduced using a rad52 mutant strain in maintenance of centromeric DNA repeat arrays. This genetic method may use more general application in the maintenance of tandem repeats in construction of HAC.
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