알코올의 섭취가 식이내 Selenium과 Vitamin E 수준을 달리한 흰쥐의 체내 Selenium 수준과 심장 및 간조직에 미치는 영향

김갑순(Kap-Soon Kim),채기수(Ki-Su Chae),정승용(Seoung-Yong Chung) 한국식품영양과학회 1994 한국식품영양과학회지 Vol.22 No.6

알코올의 섭취가 Se과 vitamin E 수준을 달리한 흰쥐의 체내 Se 수준 및 심장, 간세포 형태에 미치는 영향을 살펴보기 위하여 평균 체중이 58~62g인 Sprague-Dawley 계의 젖을 갖뗀 숫쥐 72마리를 12군으로 나누어 Se의 수준을 식이 ㎏당 0㎎, 0.4㎎, 10㎎으로 달리하고 vitamin E는 식이 ㎏당 0㎎, 150㎎으로 하여 7주간 사육시켰다. 알코올 섭취는 실험시작 3주째부터 ethanol을 급수물에 10% 맞추어 섭취군과 비섭취군으로 나누어서 섭취군에서는 제한없이 먹게 하였다. 얻어진 결과를 요약하면 다음과 같다. 혈액과 소변의 Se의 수준은 식이내 Se 수준의 차이에 따라 유의적인 차이를 보여 식이내 Se수준이 높은 H-군, 정상적으로 섭취한 C-군, 결핍된 L-군의 순으로 높았으며 알코올 섭취군들은 낮은 경향을 보였다. 간장, 신장의 Se의 농도는 식이 Se 수준에 비례하여 H-, C-, L- 순으로 높았으며 알코올섭취로 인한 Se농도의 변화는, 알코올을 섭취한 군들의 Se 농도가 낮은 경향이나 신장에서는 반대로 높은 경향이었다. 전자현미경을 통한 관찰에서 심근세포 조직형태의 변화는 식이내 Se수준이 높은 H-군들, Se수준이 낮은 L-군에서 lysosome의 증가 및 지방적 출현, mitochondria의 종창, 특히 모세혈관의 내피세포에 심한 종창이 일어났고, 알코올을 섭취한 군들에도 심근세포의 종창, lysosome의 증가, mitochondria 변화를 보였다. Se을 과잉으로 섭취하고 vitamin E를 정상으로 섭취한군(HC-)에서는 특이한 변화가 없었으나 알코올을 투여한 HCA군에서 지방적이 나타났다. 특히 Se과 vitamin E이 결핍되고 알코올을 섭취한 LLA군에서는 lysosome의 증가 및 지방적이 많이 증가하였다. The purpose of this study was to investigate the effect of alcohol administration on selenium concentration and cell morphology in tissues of rats fed with the different levels of selenium (Se) and vitamin E. Seventy two male rats of Sprague-Dawley strain weighing about 58~62g were divided into 12 groups. The dietary Se levels were 0㎎ (L-), 0.4㎎ (C-) and 10㎎ (H-), and the dietary vitamin E levels were 0㎎ (-L) and 150㎎ (-C) per kg diet, respectively. Alcohol-adminstrated groups (--A) received the triple distilled potable water solution containing 10% of ethanol from the 3rd week of experimental periods. The obtained experimental results are summarized as follows. Se concentration in blood and urine made difference in accordance with Se level in diet and tended to be low in alcohol administrated groups. Se concentration in liver and kidney was also directly proportional to the dietary Se level, and it tended to be low in each alcohol group, but Se concentration in kidney tended to be increased by alcohol administration. Myocardium in rats showed lysosome increasing, fat droplet, mitochondrial swelling, and in particular, bad intracellular edema, in H-group fed with high Se and in L-group with low Se. It also showed such phenomena in the alcohol administrated group. In HC-group fed with excessive Se and normal vitamin E, there appeared no noticeable change in liver tissue. However, in the alcohol administrated HCA-group, there came out fat droplet. Especially, in the alcohol administrated LLA-group, not fed with sufficient Se and vitamin E, there were found lysosome increasing and a number of fat droplet.

부산시내 일부 사립국민학교 부속 유치원 어린이의 영양 실태에 관한 연구 - 1. 영양 섭취실태 및 건강실태 조사 -

김갑순(Kap-Soon Kim),이성호(Sung-Ho Lee),채기수(Ki-Su Chae),임효진(Hyo-Jin Lim) 한국식품영양과학회 1994 한국식품영양과학회지 Vol.23 No.4

부산시 주례동에 위치한 사립국민학교 부설 유치원 어린이 152명 대상으로 1993년 12윌 1일 부터 12월14일 까지 실시한 영양 실태의 조사결과를 요약하면 다음과 같다. 1) 성장발육상태는 한국 소아발육 표준치와 비교해 볼 때 표준체중, 신장, 두위, 흉위의 백분율이 113.1%, 102.2%, 103.3%, 101.7%로 나타났다. 2) 4~6세 연령에 합당한 신장ㆍ체중 지표인 WLI(Weight Length Index) 결과 4세의 경우 과체중 25%, 비만 4%, 5세의 경우 과체중 25%, 비만 19%, 6세의 경우 과체중 28%, 비만 26%로 비만율이 상당히 높았다. 3) 헤마토크릿치는 4세~6세 평균 37.6%이고 헤모글로빈 농도는 12.4~12.8%로서 한국 소아 표준치 보다 높으며 적혈구, 백혈구 혈소판 수치는 정상 수준이었으며 빈혈로 간주 되는 어린이는 1명에 불과하였다. 혈액내 콜레스테롤 수치는 동맥경화의 위험 수준인 200㎎/dl를 넘는 어린이가 8%였고 중성지방은 120㎎/dl가 31% 혈당치는 공복시 100㎎/dl 이상인 어린이가 6%로 나타나 비만의 합병증인 당뇨, 동맥경화, 심혈관계 질환의 위험인자를 내포하고 있는 어린이가 많은 것으로 나타났다. 4) 조사된 어린이의 영양소 섭취량은 4세의 섭취량을 제외한 모든 섭취량들이 영양권장량의 108.4% 부터 266%로 상당히 높으며 탄수화물, 지방, 단백질의 구성비는 57.3~61.3 : 21.5~24.3 : 17~18.4로서 서구화됨을 알 수 있었다. 5) 조사 어린이의 영양섭취량과 성장발육, 혈액검사 결과 및 WLI 등의 상관관계는 매우 유의적으로 높았으며, 비만도가 높을수록 영양소 섭취량이 많다는 것을 알 수 있었다. A nutrition survey of 152 children, in the kindergarten attached to a private elimentary school of Pusan, was undertaken between Dec. 1 and Dec. 14, 1993, to investigate dietary intake and nutritional status. The results are summarized as follows. 1) Compared with the Korea child development standard, their development conditions showed 113.1 %,102.2%, 103.3%, 101.7% in weight, height, head and chest, respectively. 2) By the standard of WLI (Weight Length Index), proper to the children between age 4 and 6, there proved to be 25% overweight and 4% obesity, 25% overweight and 19% obesity, 28% overweight and 24% obesity in 4-year-old, 5-year-old, and 6-year-old children, respectively. 3) The children between age 4 and 6 averaged 37.6% in hematocrit and 12.4~12.8% in hemoglobin. These values were higher than those of the Korea child development standard. But the numbers of erythrocyte, leucocyte and platelets were normal. Only a single child was considered suffering from anemia. The 8% of them were over 200㎎/dl in cholesterol, dangerously approaching the atherosclerosis. The 31% averaged 120㎎/dl in triglyceride. And the 6% proved 100㎎/dl in blood glucose when hungry. So it came out that not a few children had some factors dangerous to diabetes, atherosclerosis and heart diseases : complications arising from obesity. 4) The intake of nutrients, except the 4-year-old children, ranged 108.4% to 266% from the recommended value. The rates of carbohydrate, fat and protein were 57.3~61.3 : 21.5~24.3 : 17~18.4. It means that they are westernized more than before. 5) There proved to be much correlation among total intake of nutrients, development, results of blood test and WLI. The degree of obesity was proportionate to the intake of nutrients.

밀기울배지를 이용한 Bacillus macerans의 Cyclodextrin Glucanotransferase 생산과 효소특성

선우양일(Yang-Il Sunwoo),안태진(Tae-Jin Ahn),김동환(Dong-Hong Kim),박인호(In-Ho Park),채기수(Ki-Su Chae),김광(Kwang Kim) 한국생물공학회 1994 KSBB Journal Vol.9 No.5

Bacillus brevis P176-2의 형질전환에 의한 α-amylase 생산균주 선발

채기수 慶南專門大學 1996 論文集 Vol.24 No.1

EFFECT OF ENVIRONMENTAL MUTAGENS ON DNA DAMAGE AND REPAIR MECHANISM

CHAE,KI SU,LEE.CHUN BOK,JUNG,JUNG NAM,LEE,HYE JOO,UM,KYUNG IL 東亞大學校附設基礎科學硏究所 1987 基礎科學硏究論文集 Vol.4 No.1

The effects of aphidicolin, an inhibitor of DNA polymerase α, on ethyl methanesulfonate (EMS)-or bleomycin (BLM)-induced DNA repair were investigated in chinese hamster ovary (CHO)cells. The amount of unscheduled DNA synthesis induced by EMS or BLM was increased according to dose increment and decreased with incubation time after treatment, and the damage almost seemed to be recovered during 4 hour incubation. The EMS-induced DNA single-strand breaks showed dose dependent increment and were increased according to EMS containing time. BLM also showed dose dependent increment on DNA single- strand breaks. On the other hand, it was shown that aphidicolin inhibited excision repair induced by EMS, and thus, the post-treatment with aphidicolin induced the higher degree of unscheduled DNA synthesis and the more accumulation of DNA single-strand breaks than the cells post-incubated without aphidicolin. However, aphidicolin did not inhibit excision repair induced by BLM, and thus, the cells post-incubated with or without aphidicolin for 1 hour had the same value in the amount of unscheduled DNA synthesis and of DNA single-strand breaks. These results suggested that the participation of DNA polymerase α in DNA repair induced by EMS or BLM was dependent on different types of damage.

전기천공법에 의한 Bacillus brevis P176-2의 형질전환

채기수,엄경일 東亞大學校附設基礎科學硏究所 1993 基礎科學硏究論文集 Vol.10 No.1

균체외단백질 생성균 Bacillus brevis P176-2 균주를 pUB110 plasmid DNA로 전기천공법에 의한 형질전환을 시도하여 본 방법의 유효성과 최적조건을 검토하였다. 본 실험에 사용한 전기천공 장치로 전장강도 12.5kV/㎝, capacitor 용량 7.3㎌의 조건하에서 고전압 pulse를 세포에 가했을 때 가장 높은 형질전환율(1.26× 10⁴/㎍ plasmid DNA)을 나타내었으며 세포 생존율은 10% 정도이었다. 세포의 생육도가 ??에서 약 1.2정도로 증식하였을 때 세포를 수확하여 형질전환 시키는 것이 가장 좋았으며 그 이상의 생육도에서는 세포의 생존율은 증가되었지만 형질전환율은 낮아졌다. 전강강도 12.5kV/㎝, capacitor용량 7.3㎌ 조건의 pulse를 세포에 2회 연속적으로 가할 때 최대의 형절전환율(2.40× 10⁴/㎍ plasmid DNA)을 보였다. 세포의 생존과 pulus의 지속시간에 영향을 주는 electroporation burrer는 7 mM HEPES(pH 7.4), 272mM sucrose 및 1mM MgCl₂을 함유하는 buffer를 사용하여 세포를 수확하고 현탁하여 형질전환할 때 형질전환율과 세포 생존율이 가장 높았다. 세포현탁액에 첨가되는 plasmid DNA의 농도가 10㎍/ml일 때 까지는 농도에 비례하여 형질전환율이 높아졌으나 그 상의 농도에서는 비례하지 않았다. The optimum conditions and mechanisms for the plasmid-mediated genetic transformation of intact cells of bacillus brevis P176-2, an extracellular protein producing bacterium, by electroporation were investigated. It was found that pUB110 plasmid DNA can be introduced into intact bacterial cells by electroporation. The frequency of transformation by this electroporation system depended upon the initial electric field strength, the capacity of the clectric discharge capacitor, growth stage, number of successive pulses and composition of electroporation buffer. It was effective for transformation that cells were harvested, washed and resuspended with HSM[7mM HEPES(pH 7.4), 272mM sucrose, 1mM MgCl₂] electroporation buffer when cell growth was attained to 1.2 at ?? A maximum frequency of transformation of 2.40× 10⁴ transformants per ㎍ plasmid DNA was obtained by two succesive pulses with an initial electric field strength of 12.5kV/㎝ and with a capacitance of 7.3㎌. Key words : Bacillus breves, pUB110, plamid DNA, transformation, electroporation.

전기천공법에 의한 Bacillus brevis P176-2의 형질전환

채기수,엄경일 한국식품영양학회 1992 韓國食品營養學會誌 Vol.5 No.2

The optimum conditions and mechanisms for the plasmid-mediated genetic transformation of intact cells of Bacillus brevis P176-2, an extracellular protein producing bacterium, by electroporation were investigated. It was found that pUB110 plasmid DNA can be introduced into intact bacterial cells by electroporation. The frequency of transformation by this electroporation system depended upon the initial electric field strength, the capacity of the electric discharge capacitor, growth stage, number of successive pulses and composition of electroporation buffer. It was effective for transformation that cells were harvested, washed and resuspended with HSM [7mM HEPES(pH 7.4), 272mM sucrose, 1mM MgCl_2] electroporation buffer when cell growth was attained to 1.2 at OD_660. A maximum frequency of transformation of 2.40×10 exp (4) transformants per ㎍ plasmid DNA was obtained by two succesive pulses with an initial electric field strength of 12.5㎸/㎝ and with a capacitance of 7.3㎌.

균체외단백질 생산균 Bacillus brevis P176-2 균주의 동정과 배양조건

채기수,엄경일 東亞大學校大學院 1991 大學院論文集 Vol.16 No.-

Identification and influence of the cultural conditions upon extracellular protein production by Bacillus brevis P176-2 isolated from soil were investigated. The morphological, physiological and cultural characteristics of strain P176-2 were studied according to the methods of Bergey's manual. Based on the results obtained in thses experiments, strain P176-2 was identified as Bacillus brevis. The extracellular protein ws efficiently produced when added to 1.0% peptone as nitrogen source. Optimum initial pH and temperature for extracellular protein production were 7.5 and 40℃ respectively. Higher concentration of magnesium ion stimulated growth, but prevented extracellular protein production. Optimum concentration of potassium phosphate was found to be 0.2%. This strain produced 5.38mg/ml of extracellular protein when cultured for 48 hours under optimum conditions. The extracellular protein produced under optimum conditions contained relatively large amounts of aspartic acid, lysine and arginine, but cystine and methionine were very low.

호알칼리성 Bacillus sp. C-21에 의한 Cyclodextrin Glucanotransferase의 생산

강희정,채기수,선우양일 한국식품영양학회 1995 韓國食品營養學會誌 Vol.8 No.3

Cyclodextnn glycosyltransferase(CGTase) 생산 균주 선별배지를 사용하여 토양으로부터 CGTase생산성이 우수할 균주를 분리하여 동정한 결과 pH 7.0∼12.0에서 생육할 수 있는 호알칼리성 Bacillus sp.이었다. 이 균주는 1.0% soluble starch, 1.0% peptone, 0.5% yeast extract, 0.1% K_2HPO_4, 0.02% MgSO_4·7H_2O, 1.0% Na_2CO_3의 조성을 가지는 배지에서 초기 pH 10.0, 배양온도 33℃로 하여 30시간 동안 jar fermentor에서 통기배양하였을 때 최대의 증식도와 효소 생산량을 나타내었으며 이 때 효소의 역가는 4.86unit/㎖이었다. 배양 상등액으로부터 acetone 침전법으로 조제한 조효소약으로 효소적 특성을 살펴본 결과 최적 활성온도는 60℃이었고 온도 안정성은 50℃까지 안정함을 보였다. 그리고 최적 활성 pH는 6.0 이었으며 pH 안정성은 pH 6.0∼9.6의 범위에서 안정하였다. A strain of alkalophilic Bacillus sp. C-21 has been isolated from soil. The strain was capable of producing large amount of cyclodextnn glycosyltransferase(CGTase) in the high alkaline pH medium. The preferable medium composition was determined to be as follows: 1.0% soluble starch, 1.0% peptone, 0.5% yeast extract, 0.1% K_2HPO_4, 0.02% MgSO_4·7H_2O and 1.0% Na_2CO_3(pH 10.0). The highest enzyme production was observed after 30hours of cultivation at 33℃. The optimum temperature and pH for the activity of crude enzyme were 60℃ and 6.0, respectively. The enzyme was stable between pH 6.0 and 9.6, and up to 55℃.

외식사업학과 교과과정 개발에 관한 연구

김두진,채기수 한국식품영양학회 1996 韓國食品營養學會誌 Vol.9 No.3

본 연구는 이론과 실무를 겸비한 중·소 외식업소의 경영자를 양성하기 위한 외식사업과의 교과과정을 개발하기 위한 결과이다. 외식업계의 직무분석과 외식관련과의 교과과정에 대한 분석 및 설문지에 의한 조사로 외식사업과의 모형 교과과정을 개발하였으며, 개설하려는 교과목들은 1) 식품과 식품위생에 관련된 과목, 2) 조리에 관련된 과목, 3) 식당경영과 서비스에 관련된 과목, 4) 실무를 익힐 수 있는 과목과, 5) 외국어 관련 과목들이다. 외식사업과를 개설하였을 때 기대되는 효과로는 1) 외식관련 전문직업인의 양성, 2) 외식관련 산업에 대한 연구의 활성화, 3) 전통 식문화의 정립, 4) 관광 산업의 활성화, 5) 외식업 종사자의 자질 향상 등이다. The purpose of this study was to develop the curriculum for the Department of Food Service Business in Junior College. The purpose of the curriculum development is to train professionals combined theory and practice for medium or small restaurant managers. We have developed a model curriculum, which was based on job analyses for related business, analyses of the curricula of departments related with food service, and survey on the questionaire. The subjects of the model curriculum are as follows. 1) Subjects related with Food and Food Sanitation, 2) Subjects related with Cuisine, 3) Subjects related with Restaurant Management and Service, 4) Subjects related with training in practical business, and 5) Subjects related with a foreign langage. The effects on opening the Department of Food Service are as follows. 1) To train professionals for food service business, 2) Activation of the study on Food Service Industry, 3) Establishment of the traditional food culture, 4) Activation of the Tourist Industry, and 5) To improve the quality of restaurant employees.