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U-937 세포에서 도적승기탕(導赤承氣湯) 추출물 중 부탄올 분획에 의한 Apoptosis 유도
박평범,정한솔,김호,진재호,정상훈,한웅,이문원,이광규,Park, Pyeong-Beom,Jeong, Han-Sol,Kim, Ho,Jin, Jae-Ho,Jeong, Sang-Hun,Han, Ung,Lee, Moon-Won,Lee, Kwang-Gyu 대한동의생리학회 2006 동의생리병리학회지 Vol.20 No.6
To investigate the anti-cancer effects of n-butanol fraction of DoJeokSeungKi-Tang extracts(nBFD) in U-937 cells. MTT assay was used to determine U-937 cells proliferation. Flow cytometry was used to detect apoptosis. Bcl-xl anti-apoptotic protein and caspase-3, p53 pro-apoptotic protein were examined by Western blot analysis. nBFD inhibited the proliferation of U-937 cells in a dose-dependent manner. The cells treated with nBFD showed a typical apoptotic process by increasing sub-Gl peak. nBFD reduced uptake of 3,3'dihexyloxacarbocyanine iodide(DiOC6) a fluorochrome which incorporates into cells dependent upon their mitochondrial transmembrane potential$({\triangle}{\psi}m)$. nBFD induced in U-937 cells apoptosis mainly via increasing sub-Gl peak, regulation of Bcl-xl, caspase-3 and p53 protein.
봉약침과 홍화약침으로 치료한 레이노 현상을 동반한 수근관증후군 환자 1례
최석우,박평범,오성종,Choi, Seok-Woo,Park, Pyeong-Beom,Oh, Sung-Jong 대한약침학회 2009 Journal of pharmacopuncture Vol.12 No.1
Objectives : This study is to report the effect of Pharmacopuncture therapy on a patient suffering from the pain and cold intolerance of hand caused by Carpal tunnel syndrome with Raynaud's phenomenon. Methods : We had treated the patient diagnosed as Carpal tunnel syndrome with Raynaud's phenomenon by Sweet BV and CF pharmacopuncture. We injected Sweet BV and CF into acupuncture points on both hands - Sweet BV into Baxie (EX-UE9), CF into Naegwan ($PC_6$) and Daereung ($PC_7$). And then we evaluated her symptoms by VAS (Visual Analog Scale). Results : Clinical symptoms about Carpal tunnel syndrome with Raynaud's phenomenon were remarkably improved by Sweet BV and CF Pharmacopuncture. Conclusion : Therefore, we concluded that pharmacopuncture therapy - Sweet BV, CF etc. - may be useful to treat Carpal tunnel syndrome with Raynaud's phenomenon.