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3D 가상심장을 이용한 심방세동의 complex fractionated atrial electrogram (CFAE) 분석 및 catheter ablation
박진서(J.S.Park),권순성(S.S.Kwon),김준우(J.W.Kim),이현승(H.S.Lee),지윤철(Y.C.Ji),황민기(M.K.Hwang),박희남(H.N.Pak),심은보(E.B.Shim) 대한기계학회 2013 대한기계학회 춘추학술대회 Vol.2013 No.12
This study analyzed complex fractionated atrial electrogram (CFAE) and CFAE ablation for atrial fibrillation patient. We modeled atrial fibrillation by a 3D virtual heart. For this purpose, measurement techniques of cardiac ablation using catheter was numerically implemented by a simulation method, and FIR filtering was used to analyze electric signal pattern measured. Through these methods, CFAE values was calculated in the 3D mapping at virtual heart, and we found ablation target of atrial fibrillation patient. This study also simulated CFAE and CFAE-based ablation for 20 patients and the computed results were compared with actual clinical data.
이현승(H.S. Lee),권순성(S.S. Kwon),박진서(J.S. Park),지윤철(Y.C. Jee),황민기(M.G. Hwang),박희남(H.N. Pak),심은보(E.B. Shim) 대한기계학회 2013 대한기계학회 춘추학술대회 Vol.2013 No.12
To represent atrial fibrillation (AF) we use the bi-domain model coupled with the Courtmanche model of human atrial cell and simulate the 3D electric waves on left atrial surface. S1-S2 stimulation protocol is applied to induce AF in the model. We obtained patient-specific geometries of left atrium (LA) from CT data and constructed three-dimensional (3D) simulation models. The purpose of this study is to investigate the effect of nerve activity on electric wave propagation in AF. In the cell model, we add acetycoline-activated potassium current to reflect the nerve effect of ganglionated plexus. Parasympathetic actions are formulated based on the experimental results of acetylcholine effects in patch-clamp studies. Computed results show that ach-activated potassium current by parasympathetic stimuls induces maintained AF waves than control condition.
전압, 공핵배 발생단계 및 체외배양액이 소의 제2세대 핵이식배 발생능에 미치는 영향
정영채,김창근,나광빈,김광식,박세영,류재원,오신애,박진서 中央大學校 遺傳工學硏究所 1998 遺傳工學硏究論集 Vol.11 No.1
소에서 체외에서 생산된 제1세대 핵이식배를 다시 공핵배로 사용하여 제2세대 핵이식배를 생산하기 위한 효과적인 방법을 개발하기 위하여 본 연구에서 세포융합시 전압, 제1세대 핵이식배의 발생단계 및 체외배양조건에 따라 제2세대 핵이식배의 발생결과를 조사한 결과는 다음과 같다. 1. 세포융합시 전압을 0.7kV/cm, 1.0kV/cm 및 1.2kV/cm로 하였을 때 세포융합율과 난할율에서 유의차는 없었으며 후기배의 발생율이 1.0kV/cm가 0.7kV/cm와 1.2kV/cm보다 다소 높게 나타났다. 2. 8-15, 16-23 및 25-32세포기의 제1세대 핵이식배를 공핵배로 이용한 제2세대 핵이식배의 융합율, 난할율 및 상실배까지의 발생율은 세포기에 따라 유의차가 없었다. 그러나 배반포배 발생율은 각각 3.4%, 6.9% 및 18.5%로서 16-23세포기와 25-32세포기간에는 유의차가 없었으나 25-32세포기는 8-15세포기보다 유의적으로 높았다(p<0.01). 3. 제2세대 핵이식배를 BOEC, BOEC-CM 및 10% FBS+BOEC-CM에서 배양한 결과 난할율과 상실배까지의 발생에서 큰 차이가 없었으나 배반포까지의 발생율은 각각 12.5%, 5.7% 및 9.6%로 BOEC 처리구에서 처리구가 가장 높은 것으로 나타났다. This study was carried out to investigate the effect of the electric pulses, donor embryo cell-stages and culture media on the 2nd generation nuclear transplant bovine embryos. Parent donor embryos were used at day 4 after IVF and 8 to 32 cell-stage embryos at day 3∼5 after the first cycle nuclear transfer were used as donor for 2nd generation. The recipient oocytes were enucleated 22 to 23 h after the onset of in vitro maturation(IVM) and preactivated 24 h after IVM. Electric fusion was performed 30 h after IVM. When a pulse of 0.7kV/cm, 1.0kV/cm or 1.2kV/cm direct current for 70μsec was applied, the fusion rates of the 2nd generation were 73.5%, 87.3% and 83.6%, respectively and the cleavage rates were 78.0%, 80.6% and 86.6%, respectively. The proportions of fusion and cleavage were lower in 0.7kV/cm than in 1.0kV/cm and 1.2kV/cm. The developmental rate to blastocyst in the 2nd generation nuclear transplant embryos was higher in 1.0kV/cm(12.9%) than in 0.7kV/cm(8.0%) and 1.2kV/cm(6.5%). The rate of fusion and cleavage and developmental rate to blastocyst in the 2nd generation embryos derived from 8 to 32 cell-stage donor embryos was 87.1%, 85.2% and 18.5%, respectively, from 25-32 cell-stage, 86.6%, 79.3% and 6.9%, respectively, from 16-23 cell-stage, and 84.1%, 77.6%, and 3.4%, respectively, from 8-15 cell-stage. These rates were not significantly different between 25-32 cell-stage and 16-23 cell-stage donor embryos, but the developmental rate was higher from 25-32 cell-stage donor embryos, than from 8-15 cell-stage. The developmental rate to blastocyst in the 2nd generation nuclear transplant embryos was higher in co-culture with bovine oviduct epithelial cells(12.5%) than in BOEC-conditioned medium with 10% FBS(9.6%) or without FBS(5.7%). The developmental rate in co-culture with BOEC was higher than in BOEC-conditioned medium.