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민정기,윤호영,이승훈,정승환,박태규,한동철 한국공작기계학회 2008 한국공작기계학회 춘계학술대회논문집 Vol.2008 No.-
Hydrodynamic focusing or sheathflow focusing has been used to confine the sample within a certain volume of a fluid stream. Using that focusing method, flowcytometers equipped with microchips should have microchannels which are horizontal to the ground. Accordingly, the sedimentation phenomena of cells which lead to the erroneous counting of cells or the problem of clogging with cells around the channel entrance are inevitable. Three-dimensional(3D) hydrodynamic focusing can solve those problems with ease. This paper presents a simple microchannel design for an effective 3D focusing of the sample in microchip type flowcytometers and experimental results of CD4+ T-cells counting using our proposed microchip for HIV screening