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香砂平胃散 및 當歸活血湯이 膽道結紮과 Dimethylnitrosamine으로 誘發된 白鼠의 肝硬變症에 미치는 影響
박대영,姜秉淇 圓光大學校 韓醫學硏究所 1995 원광한의학 Vol.5 No.1
This study was to investigate the protective and anticirrhotic effects of Hyangsapyungwuisan and Dangguishalheoltang on the liver cirrhosis or fibrosis induced by prolonged bile duct ligation; a new experimental model for cirrhosis and the intraperitoneal injection of dimethylnitrosamine in the rat. The development of fibrosis or cirrhosis and its inhibition by the two prescriptions were examined by the chemical analysis of AST, ALT, and hydroxyproline, and histological and immunohistochemical observations. The results obtained were as follows. 1. The increase of serum asparate aminotransferase induced by bile duct ligation was inhibited by the administration of Hyangsapyungwuisan and Dnagguiwhalheoltang, especially markedly inhibited in the group of Dangguiwhalheoltang administration. 2. The increase of serum alanine aminotransferase induced by bile duct ligation in rats was inhibited by the administration of Hyangsapyungwuisan and Dangguiwhalheoltang, especially markedly inhibited in the group of Dangguiwhalheoltang administration. In conclusion, the increass of AST, ALT induced by bile duct ligation was efficiently decreased by the administration of Dangguiwhalheoltang extract. 3. The increased level of serum AST and ALT induced by the intraperitoneal injection of dimethylnitrosamine was inhibited by the administration of Hyangsapyungwuisan and Dangguiwhalheoltang extract. But there was no significant differences in the inhibition rate. 4. The incerasing level of hydroxyproline volume in the damaged lever tissues in rat were decreased by the oral administgration of Hyangsapyungwuisan and Dangguiwhalheoltang extract. But there was no significant differences in the inhibition rate between the two experimental groups. 5. The histological observation the damaged liver tissue and immunohistochemical observation of bromodeoxyuridine in the liver tissue of rats showed the significantly hepatoprotective effects of Hyangsapyungwuisan and Dangguiwhalheoltang extract, and resealed the regenerative effects of Hyangsapyungwuisan and Danggui- whalhioltang etract on the damaged liver induced by bile duct ligation and the i.p.injection of dimethylnitrosamine.
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