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다발성 원발성 악성종양 : 이중복암 (二重複癌) 1예 보고 A case of double primary malignant cancer
박광숙,윤종만,장건성,국돈표,윤영근,김성열 대한소화기내시경학회 1983 Clinical Endoscopy Vol.3 No.1
This is a case report of double primary malignant cancer occurred aynchronously in the stomach and lymphoid tissue, We report this case with review of literatures about the criteria, age distribution, predisposing factor, inidence, immunity and susceptibility of the primary malignant neoplasms. This case was a 59-year-old man who had Hodgkins disease and tubular adenocarcinoma, of the stomach. The diagnoais was verified histologically, Although multiple primary neoplaas are rare, the possibility of that must be conaidered seriously, And its hereditary predisposition and other predisposing factor muat be researched with enthuaiasm.
Megakaryocyte-specific gene platelet factor 4에 대한 분자 생물학적 이해
박광숙 한국생화학분자생물학회 1991 생화학분자생물학회 소식 Vol.11 No.2
This paper will be introduced recent advances in the molecular biology of platelet factor 4 (PE4). Since PF4 has been reported to be an immunological regulator by inhibiting suppressor T cell activity and to have an autocrine effect of megakaryocyte differentiation. I also discuss our research at expressing a recombinant PE4 (rPE4) and our present progress in studying its biological properties, as well as the cloning and organization of the human PF4 and a highly homologous gene PF4alt. PF4 is a 70 amino acid protein released from the alpha graules of platelets following activation. The exact biological function of this protein is unknown. PF4 binds to heparin with high affinity. This property allowed initial purification of PF4 from platelets, and may reflect its key biological role. By neutralizing heparin. PF4 may inhibit heparin-induced anti-thrombin 111 activation. and permit platelets to bind to endothellial cells, thus supporting the early phases of coagulation. PF4 is also a strong chemoattractant for neutrophils, monocytes and fibroblasts. It binds to platelet surfaces, and permits platelet activation at lower agonist concentrations. Recently, studies have suggested that PF4 may also effect megakaryocyte maturation in the marrow as a negative autocrine. A repeating structure of two lycinoe followed by two hydrophobic amino acid residues in mature protein is present near the C-terminus. Based on studies using short C-terminal oligopeptides these lysine repeats are thought to be critical for PF4's heparin binding and chemoattractant properties. We have previously cloned the cDNA for human PF4 from a human erythroleukemic (HEL) cell expression library and showed that the inital translated product is 100 amino acids in length consisting of a 30 amino acid residue signal peptide followed by the 70 amino acid mature protein. These studies also demonstrated that in HEL cells, the PF4 mRNA is approximately 800 nucleotides (n) long. PF4 also appears to have a number of other properties in uitro whose biological significance is unknown. Since some of these properties require fairly high concentrations of PF4, it is also possible that some of these activities may be due to contaminating proteins. One of the values of synthesizing rPF4 is to confirm proposed biological functions of the native protein. We have constructed an expression vector for rPF4 in the T7-based promoter vector pT7-7, to better the relationship between PF4 structure and function. The protein was expressed in E. coli and purified to homogeneity. Biological studies have demonstrated that rPF4 has identical neutrophi1 chemotactic properties as the native protein. These support PF4's role as an important chemotactic agent in the human body. Site-directed mutations of rPF4 will further demonstrate whether these many biological properties depend on the C-terminal region of PF4 which contains heparin-binding activity. Initial studies with rPF4 as a negative autocrine agent in megakaryocyte development also suggest that rPF4 has identical biological function to native PF4 in regulating megakaryocyte development, supporting the conclusion that the negative effect seen with native PF4 is not due to a contaminating factor. Furthermore, over expression vector should prove useful for the construction of recombinant forms of PF4 to investigate structure function relationships of this biologically important protein.
박광숙,김성렬,박종춘,김석빈,윤종만,국돈표 대한소화기내시경학회 1984 Clinical Endoscopy Vol.4 No.1
Any foreign body in the esohagus means an acute danger because of the impending perforation followed by mediastinitis, the impending erosion of bigarteries, and impending aspiration in the care of esorhageal occlusion. Nowadays, foreign body can reliably be removed hy endoscopy, and do not perforate the wall, not changed their form. Complication at the endoscopic extraction of foreign body, that requre surgical intervention are very rare. We presented a case of esophageal foreign body complicated with esophageal ulcer in a 83 years old male.