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일반 배양액과 Vero cell 공배양에서의 생쥐 배아 발달에 관한 비교 연구
이유성(You Sung Lee),이창호(Chang Ho Lee),고희정(Hee Jung Go),이기숙(Ky Suk Lee),류철희(Chul Hee Rheu),김종덕(Jong Duk Kim) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.6
목적 : 인간 체외수정에 적용하는 공배양 기법에 사용할 세포로 vero cell을 택하여 vero cell monolayers 상에서 생쥐배아를 공배양한 것과 일반배양액인 IVF-20 배양액에서만 생쥐배아를 배양하여 두군의 배아양상에서 배아 발달을 비교하여 그 차이를 밝혀보고자 하였다. 방법 : 생쥐 암컷의 난포성장을 촉진시키기 위해 6~8주 된 흰 생쥐 암컷에 PMSG 5 IU를 복강주사 한 후 48시간 후에 hCG 5 IU를 다시 복강 주사하여 암수를 합사시켰으며, 교미가 일어난 암컷으로부터 난관을 무균적으로 절취하여 배아를 채취하였고, 난할이 일어난 정상적이고 건강한 2세포기 배아만을 각 실험에 사용하였다. 생쥐 2세포기 배아를 일반 배양액인 IVF-20에서만 배양하여 보고, 또한 vero cell과 공배양하여 각각의 발달 정도를 조사하였다. 결과 : 1. 배양 초기인 48시간까지는 일반배양액인 IVF-20에서만 배양한 것이 배아발달율이 높아 4세포기 (4 cell)까지는 IVF-20에서 67.0%, 상실배 (moula)까지는 55.7% (p<0.05)의 발달율을 보였으나, vero cell 공배양에서 는 4세포기까지 47.6%, 상실배까지는 42.9%의 발달율만을 보였다. 2. 배양중기인 72시간에는 IVF-20 배양액에서 배양하였을 때에는 배반포 (blastocyst)와 확장된 배반포 (expanded blastocyst)까지 51.6% (p<0.01)의 발달을 보였으며, vero cell과 공배양하였을 때에는 25.9%의 발달을 보였다. 3. 배양후기에 들어서면서 배양 96시간에는 확장된 배반포와 부화까지 IVF-20에서 배양하였을 때에는 37.7%의 발달을 보였고, vero cell과 공배양하였을 때에는 32.6%의 발달을 보여 두군 사이에 비슷한 발달율을 보였다. 4. 배양 120시간에는 확장된 배반포와 부화까지 IVF-20에서는 36.9%, vero cell과 공배양 했을때는 37.4%를 보여 비슷한 발달을 보였다. 결론 : 배양 초기에는 vero cell 공배양에서 보다 일반 배양액인 IVF-20에서 더 빠른 그리고 더 좋은 발달율을 보이며, 배양후기에 들어 확장된 배반포와 부화까지 발달하는 율은 IVF-20에서와 vero cell 공배양에서 비슷한 배아 발달율을 보임을 알 수 있었다. Objective : To evaluate mouse embryos development in conventional medium IVF-20 versus vero cell coculture. Methods : Female ICR mice aged 6 to 8 weeks, were stimulated with 5IU PMSG and 48 hours later were injected 5IU of hCG, then female and male mice were mated. At 48 hour post-hCG injection, oviducts were dissected out and 2-cell embryos were flushed. The 2-cell embryos were cultured in IVF-20 media or media containing vero cell (African green monkey kidney epithelial cell lines) for 120 hours. Coculture techniques have been applied in mouse 2-cell embryos culture used vero cell lines. Results : 1. After 48 hours culture, 60.7% and 55.7% of 2 cell embryos developed to 4 cell and morulae stage, respectively, in IVF-20 culture medium, but significantly less embryos developed to 4 cell (47.6%, p<0.05) and momlae (42.9%, p<0.05) in vero cell coculture. 2. After 72 hours culture, 51.6% of 2 cell embryos developed to blastocyst and expanded blastocyst in IVF-20 culture medium, but significantly less embryos developed to blastocyst and expanded blastocyst (25.9%, p<0.01) in vero cell coculture. 3. After 96 hours culture, 37.7% and 32.6% of 2 cell embryos similar developed to expanded blastocyst and hatching in IVF-20 culture medium and vero cell coculture, respectively. 4. After 120 hours culture, 36.9% and 37.4% of 2 cell embryos similar developed to expanded blastocyst and hatching in IVF-20 culture medium and vero cell coculture, respectively. Conclusion : There was no difference of embryo development rates between the two culture groups. IVF-20 medium alone gives a benefit to the viability of an embryo compared with a vero cell coculture.
호르몬 대체요법시 사용된 약제에 따른 혈중 지질대사 및 골밀도의 변화
김현영 ( Hyun Young Kim ),김성욱 ( Sung Yk Kim ),안은준 ( Eun Jun Ahn ),류철희 ( Chul Hee Rheu ),김종덕 ( Jong Duk Kim ) 전북대학교 의과학연구소 2005 全北醫大論文集 Vol.29 No.2
Objective: This study was to evaluate the effect of hormone replacement therapy on lipid metabolism and bone mineral density through estrogen, cyclic estrogen/progesterone combination and Tibolone. Method : 204 postmenopausal women were divided into 3 groups; hysterectomized women were treated with 0.625mg conjugated equine estrogens only/day (n=50), nonhysterecomized women were treated with 2mg estradiol valerate(E2)/day and 1mg cyproterone acetate(CPA)/day for 12 of each 28 days (n=130), or Tibolone 2,5mg/day(n=24). The effects on lipid profile and bone mineral density were evaluated at baseline and after 12, 24, 36 and 48 months of treatment. Results : Conjugated equine estrogen reduced significantly the serum LDL cholesterol levels and increased triglycerides, E2/CPA increased significantly the serum HDL cholesterol levels and serum total cholesterol levels were increased in both group without significance. Tibolone reduced markedly not only the serum total cholesterol levels, HDL cholesterol levels, and triglyceride levels, but also increased mildly the serum LDL cholesterol levels. There was no significant difference between femoral neck BMD through 48 months of therapy in the three groups, but E2/CPA increased significantly the lumbar spine BMD through 48 month of therapy. Conclusion : Each of conjugated equine estrogen, E2/CPA or Tibolone induced a different mixed changes on the lipid profile. The reduction in HDL cholesterol induced by Tibolone may be able to be offset by the substantial falls in triglycerides, total cholesterol.
임신 중 급성 충수돌기염으로 오인된 대장게실염의 보존적 치료
김선영 ( Sun Young Kim ),정영주 ( Young Ju Jeong ),류철희 ( Chul Hee Rheu ),송윤경 ( Yun Kyung Song ),채희숙 ( Hee Suk Chae ),최원구 ( Won Ku Choi ) 대한산부인과학회 2012 Obstetrics & Gynecology Science Vol.55 No.12
A 24-year-old pregnant woman was referred for further evaluation and treatment of the right lower quadrant pain at 19+1 weeks gestational age. The patient was diagnosed to be acute appendicitis and underwent laparoscopic surgery. However, an inflamed solitary cecal diverticulum was found during surgery. After a routine appendectomy, cecal diverticulitis was conservatively treated with postoperative antibiotics without signs of peritonitis. To date, most of the non-pregnant women with cecal diverticulitis have been managed surgically. Our case indicates that a pregnant patient with cecal diverticulitis could be managed medically during pregnancy.
자연 생리주기에서 발생된 병합임신 1 예 / 초음파 진단
이창호(Chang Ho Lee),백은경(Eun Kyeung Baek),정영주(Young Ju Jeong),김관식(Kwan Sik Kim),진영호(Young Ho Jin),류철희(Chul Hee Rheu),김종덕(Jong Duk Kim) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.3
Heterotopic pregnancy in a natural cycle is rare case. This case occurs by one out of 30,000 cases of pregnancies. It is quite difficult to diagnose a heterotopic pregnancy clinically. But, recently, the availability of high-resolution sonography has improved the preoperative diagnosis rate. We present a case of spontaneous heterotopic pregnancy which was diagnosedp re-operatively by transvaginal ultrasound.
박정규 ( Jeong Kyu Park ),윤석근 ( Seok Geun Yoon ),김성욱 ( Sung Ug Kim ),이정헌 ( Jeong Heon Lee ),김종현 ( Jong Hyeon Kim ),이승연 ( Seung Yeun Yi ),정소망 ( So Mang Jeong ),류철희 ( Chul Hee Rheu ),김종덕 ( Jong Duk Kim ) 대한산부인과학회 2005 Obstetrics & Gynecology Science Vol.48 No.2
목적: 자궁근종은 자궁 및 자궁부속기에서 발생하는 종양 가운데 가장 흔한 양성질환이다. 최근 5년 동안 전북대학교 병원에 내원한 사이의 부인과 환자 중에 자궁근종으로 진단된 3,259명을 대상으로 자궁근종의 최근 발생율과 진단, 치료 방법, 예후에 관한 임상적 고찰을 통해 자궁근종의 특성을 알아보고자 하였다. 연구 방법: 1998년 1월 1일부터 2002년 12월 31일까지 기간동안 전북대학교병원 산부인과에 내원한 환자 중에서 자궁근종으로 진단받은 환자 From January 1998 to December 2002, 3,259 cases of uterine myoma were treated at the department of Obstetrics and Gynecology, Chunbuk National University Hospital. A clinico-stastical study of uterine myoma was perfomed to analyse the clinical characteris
김선영 ( Sun Young Kim ),진세진 ( Se Jin Jin ),조동휴 ( Dong Hyu Cho ),장규윤 ( Kyu Yun Jang ),정영주 ( Young Ju Jeong ),류철희 ( Chul Hee Rheu ),오병찬 ( Byung Chan Oh ) 대한산부인과학회 2008 Obstetrics & Gynecology Science Vol.51 No.12
Hemangiopericytoma is a rare vascular tumor arising from the adventitial pericytes of the small capillaries. Hemangiopericytomas primarily affect adults and are rare in infants and children. Both genders are equally affected. The most common anatomic locations for hemangiopericytoma are the lower extremity, axilla, pelvis, retroperitoneum, and head and neck. Surgical resection is the treatment of choice for hemangiopericytoma. The occurrence of this tumor in the vulva is rare, and to our knowledge we report the first case of a hemangiopericytoma arising from the vulva in Korea.
생쥐 난자의 체외 성숙과 수정에 있어서 난구 세포의 역할에 관한 연구
이희섭,류철희,이기숙,오병찬,조성남,진소자,두재균,김관식,김종덕 의과학연구소 1996 全北醫大論文集 Vol.20 No.2
The time course and conditions encessary for oocyte maturation and the role of cumulus cells on fertilization in vitro were studied in the mouse. nuclear maturation of cultured oocytes was evaluated by analyzing chromosomal configurations. Isolated oocytes from cumulus-oocyte complex were matured spontaneously in vitro, nad 81% of oocytes developed to the metaphase Ⅱafter 24 horus inculbation. Cumulus-oocyte complex matured in vitro, and 865 of those developed to the metaphase Ⅱ agter 24 hours. The fertilization frequency of isolated oocytes in hyaluronidase was similar in both +HCG and -HCG culture meticum(29.2% and 21.7%, respecitvely). However the fretilization frequency of cumulus-oocyte complex was greater than that of isolated oocytes, and the fertilization frequency of cumulus-oocyte complex cultrued in +HCG culture meticum was lower than that cultrued in -HCG culture meticum(36.4% and 58%, respectively). It is concluded that frequency of spontaneous maturation of isolated oocyte was similar to that of cumulus-oocyte complex, and that the presence of cumulus cells during fertilization may affect the oocyte's ferilization.