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쇠무릎(Achyranthes japonica Nakai)으로부터 Ecdysteroid 생합성에 관련된 유전자의 분리
김소미,김대운,채현병,조문제,류기증,부경환,진성범,이도승 한국농화학회 2001 Applied Biological Chemistry (Appl Biol Chem) Vol.44 No.3
In order to isolate genes involved in ecdysteroids biosynthesis in plants, total RNA was isolated from Achyranthes japonica Nakai, and RT-PCR was performed using degenerate primers selected based on the results of multi-alignment of four cytochrome P450 genes from plants and a putative ecdysone 20-hydroxylase gene from an insect. Fourteen partial cDNA clones showing unique base sequences were obtained, out of which six showed homologies at the levels of nucleotide and amino acid sequences to the other cytochrome P450 genes known to be involved in the ecdysteroid biosynthesis. Of the six clones, four showed relatively high homologies to a putative ecdysone 20-hydroxylase gene isolated from an insect.
식물의 Ecdysteroids 함량에 미치는 광과 식물생장조절제의 영향
김대운,채현병,조문제,류기증,부경환,진성범,이도승 한국농화학회 2001 Applied Biological Chemistry (Appl Biol Chem) Vol.44 No.3
Effects of light, methyl jasmonate(MJ), 6-benzylaminopurine(BA), thidiazuron(TDZ), and 2,4-dichlorophenoxyacetic acid(2,4-D) on the contents of ecdysteroids (β-ecdysone+polypodine B) in Polypodium vulgare L. and Achyranthes japonica Nakai were studied. When the plants of P. vulgare were cultured under light control, the ecdysteroids contents in both leaves and rhizomes decreased with increasing light intensity. The ecdysteroids contents in A. japonica were also lower when cultured under light than under dark. Among the tested plant growth regulators, MJ, BA, and TDZ increased the ecdysteroids contents in both P. vulgare and A. japonica.
유기인제에 의한 뇌 손상에 있어서 흥분성 아미노산의 역할
김동식,이봉희,이영재,김소미,김대성,이경갑,조문제,고봉우,박은혜,방성현,진주연,주창완,류기증,박민경 한국농화학회 2001 Applied Biological Chemistry (Appl Biol Chem) Vol.44 No.3
This study investigated the role of excitatory amino acid systems in the initiation of organophosphate-induced seizures and brain damages in rats through quantitative in viva microdialysis. Microdialysates were collected from the hippocampus of rat brain, treated with diisopropylfluorophosphate (DFP; 2.67 ㎎/㎏, s.c.) alone, and/or atropine sulfate (15 ㎎/㎏, i.m.) and procyclidine (30 ㎎/㎏, i.m.). The protective effects of atropine, a muscarinic blacker, and/or procyclidine, a N-methyl-D-aspartate and cholinergic antagonist, against DFP were examined. DFP treatment increased the levels of aspartate (Asp) and glutamate (Glu) significantly in the hippocampal persuate with the induction of seizures. Treatment of procyclidine could effectively block the increase of Asp and Glu levels. Atropine treatment showed no significant anticonvulsive effects against DFP-induced seizures. The increases of Asp and Glu levels by DFP were also completely blocked through the combined treatment of atropine and procyclidine. Histopathological findings on the hippocampus confirmed the above results. More effective protection was observed through the treatments of procyclidine alone or of both procyclidine and atropine than atropine alone against DFP-induced brain damage. Procyclidine was shown to be effective in DFP-induced seizures.