쇠무릎(Achyranthes japonica Nakai)으로부터 Ecdysteroid 생합성에 관련된 유전자의 분리

김소미,김대운,채현병,조문제,류기증,부경환,진성범,이도승 한국농화학회 2001 Applied Biological Chemistry (Appl Biol Chem) Vol.44 No.3

In order to isolate genes involved in ecdysteroids biosynthesis in plants, total RNA was isolated from Achyranthes japonica Nakai, and RT-PCR was performed using degenerate primers selected based on the results of multi-alignment of four cytochrome P450 genes from plants and a putative ecdysone 20-hydroxylase gene from an insect. Fourteen partial cDNA clones showing unique base sequences were obtained, out of which six showed homologies at the levels of nucleotide and amino acid sequences to the other cytochrome P450 genes known to be involved in the ecdysteroid biosynthesis. Of the six clones, four showed relatively high homologies to a putative ecdysone 20-hydroxylase gene isolated from an insect.

The Development of China’s Venture Capital Industry and the Characteristics of its Capital Spatial Configuration

김소미 한국경제지리학회 2017 한국경제지리학회지 Vol.20 No.4

This paper examines the development process of China’s venture capital industry and the characteristics of its capital spatial configuration. The results reveal that the development of China’s venture capital industry has its unique modality, which varies greatly from existing studies, namely, the desynchronized paces of industry development as well as the regional real capital supply and demand trend. More detailed observation shows the Chinese venture capital’s spatial configuration has three main characteristics: first, the separation of the registered location and the actual location of business operation; second, the division and spatial configuration of the venture fund’s capital circulation. To find the relevant factors related to the above phenomena, a correlation analysis was conducted between both market factors and non-market factors with the above capital spatial configuration. The analysis confirmed that besides the market factors, local legislative condition also presents a high correlation with the phenomena.

만성 적혈구 수혈에 대한 실태 분석

김소미,김선형 대한수혈학회 2016 大韓輸血學會誌 Vol.27 No.3

Background: Chronic red blood cell (RBC) transfusion is widely practiced as part of supportive care in patients with chronic anemia. However, there were few reports on the actual situation in Korea. Therefore, we studied the current status of chronic RBC transfusion. Methods: Among patients who received RBC transfusions at a regional hospital from January 2011 to December 2015, patients who were consistently transfused with more than 15 units of RBC for more than one year were included. We retrospectively analyzed the status of RBC transfusion, including the laboratory findings related to transfusion using medical records. Results: A total of 58 patients were included in the study. The mean age of patients was 66.4 years, and there were 24 hematologic malignancies (41.4%), 20 malignant tumors (34.5%), and 14 chronic diseases (24.1%). The mean hemoglobin level at the beginning of transfusion was 7.0 g/dL, and eight patients (13.8%) showed RBC alloimmunization as the transfusion proceeded. The mean serum ferritin level at the initial phase of transfusion was 475.3 ng/mL and increased to 1,462 ng/mL during the late phase of transfusion. Conclusion: Since the number of elderly patients treated with chronic diseases including tumors is expected to increase significantly, patients with chronic transfusions are also expected to increase. It is necessary to research and prepare measures such as establishment of guidelines. 배경: 만성 적혈구 수혈은 만성 빈혈 환자에서지지요법의 일부로 널리 시행되고 있다. 그러나국내에서의 실태에 대해서는 보고된 바가 적다. 이에 저자들은 만성 적혈구 수혈의 실태에 대해조사해보고자 하였다. 방법: 2011년 1월부터 2015년 12월까지 지역의한 의료기관에 내원하여 적혈구제제 수혈을 받은환자들 중, 1년 이상의 기간 동안 15단위 이상의적혈구를 꾸준히 수혈 받은 환자들을 대상으로하였다. 해당 환자들에서 수혈 관련 검사소견 등을 포함하여 적혈구제제 수혈 실태를 의무기록을이용하여 후향적으로 검토하였다. 결과: 대상에 포함된 환자는 총 58명이었다. 환자들의 평균 연령은 66.4세였고, 혈액종양질환 24 명(41.4%), 악성종양질환 20명(34.5%), 그 외 만성질환 14명(24.1%)이었다. 처음 수혈 시작시 혈색소 수치는 평균 7.0 g/dL였으며, 수혈이 진행되면서 적혈구 동종면역이 발생한 환자는 8명(13.8%) 이었다. 수혈 초기 혈청 페리틴 결과는 평균 475.3 ng/mL였으며, 수혈 후기에는 평균 1,462 ng/mL 로 증가되었다. 결론: 종양을 포함한 만성질환으로 치료받는고령 환자가 크게 증가할 것이므로 만성 수혈 환자 또한 증가할 것으로 예상된다. 이에 대한 연구와 가이드라인 정립과 같은 대책 마련이 필요하다.

대가 없는 친절을 베풀어준 아스따

김소미 샘터사 2019 월간 샘터 Vol.588 No.-

P-12 : The Effect of Serum Zinc on Salt Taste Acuity and Dietary Sodium Intake in Hemodialysis Patients

김소미,송현주,한수민,강신숙,이은주,이상아,강기수 한국정맥경장영양학회 2014 한국정맥경장영양학회 학술대회집 Vol.2014 No.-

Background: High sodium intake is the main cause of fluid overload in hemodialysis (HD) patients, leading to increased cardiovascular mortality. High sodium intake is known to be associated with low salt taste acuity and high salt preference. As the zinc status could influence taste acuity, we analyzed the effect of serum zinc status on salt taste acuity, salt preference, and dietary sodium intake in HD patients. Patients and Methods: This cross-sectional study enrolled 77 end-stage renal disease patients who underwent HD in Jeju National University Hospital. The patients were divided into 2 groups based on serum zinc levels: 26 patients had normal levels and 51 were zinc deficient. Salt taste acuity and salt preference were determined by a sensory test using varying concentrations of NaCl solution, and dietary sodium intake was estimated using 3-day dietary recall surveys. Results: Baseline characteristics including mean age, sex, cause of end-stage renal disease, number of antihypertensive medication, and blood pressure were not different between the 2 groups. The mean salt recognition threshold and salt taste preference were significantly higher in the zinc deficient group than in the non-zinc deficient group. There was a tendency for higher dietary sodium intake in the zinc deficient group but the difference was not significant. However, interdialytic weight gain was significantly higher in the zinc deficient group than in the non-zinc deficient group (2.68±1.02 kg vs. 3.18±1.02 kg; p=0.04). Dietary sodium intake and the serum zinc level showed a significant negative linear relationship (r=-0.313, p<0.05). Conclusion: Zinc deficiency may be related to low salt taste acuity and high salt preference, leading to high dietary sodium intake in HD patients.

Silicone 변성 EPDM 방진 고무의 제조와 그 특성

김소미,강두환,임완빈 한국공업화학회 2004 한국공업화학회 연구논문 초록집 Vol.2004 No.-

Epigenetic regulation and chromatin remodeling in learning and memory

김소미,강봉균 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-

Understanding the underlying mechanisms of memory formation and maintenance has been a major goal in the field of neuroscience. Memory formation and maintenance are tightly controlled complex processes. Among the various processes occurring at different levels, gene expression regulation is especially crucial for proper memory processing, as some genes need to be activated while some genes must be suppressed. Epigenetic regulation of the genome involves processes such as DNA methylation and histone post-translational modifications. These processes edit genomic properties or the interactions between the genome and histone cores. They then induce structural changes in the chromatin and lead to transcriptional changes of different genes. Recent studies have focused on the concept of chromatin remodeling, which consists of 3D structural changes in chromatin in relation to gene regulation, and is an important process in learning and memory. In this review, we will introduce three major epigenetic processes involved in memory regulation: DNA methylation, histone methylation and histone acetylation. We will also discuss general mechanisms of long-term memory storage and relate the epigenetic control of learning and memory to chromatin remodeling. Finally, we will discuss how epigenetic mechanisms can contribute to the pathologies of neurological disorders and cause memory-related symptoms.

Mind Bomb-2 Regulates Hippocampus-dependent Memory Formation and Synaptic Plasticity

김소미,김태현,이혜련,공영윤,강봉균 대한약리학회 2015 The Korean Journal of Physiology & Pharmacology Vol.19 No.6

Notch signaling is a key regulator of neuronal fate during embryonic development, but its function in the adult brain is still largely unknown. Mind bomb-2 (Mib2) is an essential positive regulator of the Notch pathway, which acts in the Notch signal-sending cells. Therefore, genetic deletion of Mib2 in the mouse brain might help understand Notch signaling-mediated cell-cell interactions between neurons and their physiological function. Here we show that deletion of Mib2 in the mouse brain results in impaired hippocampal spatial memory and contextual fear memory. Accordingly, we found impaired hippocampal synaptic plasticity in Mib2 knock-out (KO) mice; however, basal synaptic transmission did not change at the Schaffer collateral-CA1 synapses. Using western blot analysis, we found that the level of cleaved Notch1 was lower in Mib2 KO mice than in wild type (WT) littermates after mild foot shock. Taken together, these data suggest that Mib2 plays a critical role in synaptic plasticity and spatial memory through the Notch signaling pathway.

포스터 전시 : 신장, 혈액종양 ; enoxaparin으로 혈액투석 중이던 신부전 환자에서 발생한 출혈성 심낭 압전 1례

김소미,차병효,박길효,현진남,유우주,김석영,김나리,이선미 대한내과학회 2005 대한내과학회 추계학술대회 Vol.69 No.10

Simple Method to Estimate Daily Sodium Intake in Peritoneal Dialysis Patients

김소미,이은경,조종태,강기수,송현주,강민숙,이은주,한수민,김순배 한국정맥경장영양학회 2016 한국정맥경장영양학회 학술대회집 Vol.2016 No.-

Background: Restriction of dietary sodium intake for peritoneal dialysis (PD) patients is recommended, but there is limited information on the measurement and monitoring of sodium intake. We have developed a simple method to estimate daily sodium intake during the measurement of dialysis adequacy in PD patients. Methods: A total of 83 PD patients from the Asan Medical Center were enrolled into the study. The patients were divided into two groups based on residual renal function (RRF). We measured total sodium removal and estimated daily sodium intake using dietary recall for 1 day, during the assessment of dialysis adequacy. Results: There were 39 patients in the RRF(-) group and 44 in the RRF(+) group. In both groups, and all patients, there were significant positive correlations between sodium intake and total sodium removal: RRF(-) group, r = 0.598; RRF(+) group, r = 0.577; total patients, r = 0.595. There were linear relationships between dietary sodium intake and total sodium removal in both groups: RRF(-) group, sodium intake (mg/d) = 19.28 × peritoneal sodium removal (mEq/d) + 211; RRF(+) group, sodium intake (mg/d) = 15.40 × total sodium removal (mEq/d) + 609. All PD patients, sodium intake (mg/d) = 15.64 × total sodium removal (mEq/d) + 646. Conclusions: The measurement of total sodium removal during the assessment of dialysis adequacy could be an effective and simple method to estimate dietary sodium intake in PD patients. A dietary intake of 2,000 mg of sodium corresponds to a total sodium removal of approximately 87 mEq/d.