Hypericin, a Naphthodianthrone Derivative, Prevents Methylglyoxal-Induced Human Endothelial Cell Dysfunction

도문호,김선여 한국응용약물학회 2017 Biomolecules & Therapeutics(구 응용약물학회지) Vol.25 No.2

Methylglyoxal (MGO) is a highly reactive metabolite of glucose which is known to cause damage and induce apoptosis in endothelial cells. Endothelial cell damage is implicated in the progression of diabetes-associated complications and atherosclerosis. Hypericin, a naphthodianthrone isolated from Hypericum perforatum L. (St. John's Wort), is a potent and selective inhibitor of protein kinase C and is reported to reduce neuropathic pain. In this work, we investigated the protective effect of hypericin on MGO-induced apoptosis in human umbilical vein endothelial cells (HUVECs). Hypericin showed significant anti-apoptotic activity in MGO-treated HUVECs. Pretreatment with hypericin significantly inhibited MGO-induced changes in cell morphology, cell death, and production of intracellular reactive oxygen species. Hypericin prevented MGO-induced apoptosis in HUVECs by increasing Bcl-2 expression and decreasing Bax expression. MGO was found to activate mitogen-activated protein kinases (MAPKs). Pretreatment with hypericin strongly inhibited the activation of MAPKs, including P38, JNK, and ERK1/2. Interestingly, hypericin also inhibited the formation of AGEs. These findings suggest that hypericin may be an effective regulator of MGO-induced apoptosis. In conclusion, hypericin downregulated the formation of AGEs and ameliorated MGO-induced dysfunction in human endothelial cells.

감잎, 녹차, 회화나무 열매 추출 복합물 (BLH308)의 탈모 개선 효과

이화,도문호,이민지,오수빈,조수연,정종문 한국약용작물학회 2022 한국약용작물학회지 Vol.30 No.4

Background: Persimmon leaf (Diospyros kaki), green tea (Camellia sinensis), and sophora fruit (Sophora japonica) are traditional food sources known to prevent oxidative stress and inflammation. However, research on their effect in reducing hair loss is lacking. The purpose of this study was to determine whether an extract mading using these plants, BLH308 could help reduce hair loss. Methods and Results: The antioxidant activity of BLH308 in vitro was investigated as well as its anti-inflammatory effect in RAW 264.7 cells and its effect on the expression of 5-alpha reductase Ⅱ in human dermal papilla cells. BLH308 showed significant antioxidant activity owing to its ability to scavenge 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radicals. Moreover, BLH308 effectively inhibited the lipopolysaccharide-induced production of interleukin-6 and tumor necrosis factor-α in RAW 264.7 cells, and downregulated the expression of 5-alpha reductase Ⅱ in human dermal papilla cells. In vivo, the hair growth promoting effect was evaluated using hair growth visual scoring. BLH308 reduced hair loss in testosterone propionateinduced hair loss C57BL/6N mice by promoting the onset of growing phase of the hair growth cycle. Conclusions: Our results showed that BLH308 can promote hair regeneration and repair hair loss, and is a potential novel candidate for the treatment of androgenetic alopecia.

Modulation of Melanin Synthesis by Amaranthus spp. L Seed Extract in Melan-a Cells

서재옥,도문호,이재학,이택환,HUSSAIN MUSTATAB WAHEDI,박용운,김선여 한국생약학회 2016 Natural Product Sciences Vol.22 No.3

Anti-melanogenic effects of amaranth (AT), one of the key source of squalene, were investigated in melanocytes. Amaranth seed powder was extracted with water and melan-a cells were treated with various concentrations of AT. By using HPLC, content of myo-inositol, one of potential active components, was measured in the crude extract of AT.AT reduced the melanin content in melan-a melanocytes and down-regulated melanogenic enzyme activity such as tyrosinase, TRP-1 and TRP-2. By regulating melanogenic enzyme activity, AT may be a potential natural source for whitening agent. Myo-inositol was detected in AT by HPLC and may be one of the active compounds from AT involved in the regulation of anti-melanogenesis. In this study, we demonstrated that AT has anti-melanogenesis properties. This new function of amaranth may be useful in the development of new skin-whitening products and its value as food.

Dietary Supplementation with a Fermented Barley and Soybean Mixture Attenuates UVB-induced Skin Aging and Dehydration in Hairless Mouse Skin

권오욱,Ramu Venkatesan,도문호,지은희,조동운,이기원,김선여 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.2

This study evaluated the protective effects of dietary supplementation with a fermented barley and soybean mixture (BS) on ultraviolet (UV) B-induced photoaging in hairless mice. Skin aging-related parameters and protein levels related to skin wrinkles and moisturization in mice were analyzed. The BS reduced wrinkle formation, skin thickening, transepidermal water loss, and matrix metalloproteinases- 1 expression in skin. Skin hydration and pH were increased in the BS group. BS attenuated filaggrin expression as well as free amino acid and glycerol production. BS increased superoxide dismutase activity as well as increased expression of nuclear factor (erythroidderived 2)-like 2, procollagen type-I, and decreased erythema. These results suggest that BS protects against photoaging induced by UVB in vivo, indicating the potential of such mixtures as anti-photoaging dietary supplementation.

Long-term Treatment with Oriental Medicinal Herb Artemisia princeps Alters Neuroplasticity in a Rat Model of Ovarian Hormone Deficiency

김현범,권병재,조현지,김지원,천정우,도문호,박상용,김선여,맹성호,박유경,박지호 한국뇌신경과학회 2015 Experimental Neurobiology Vol.24 No.1

Artemisia princeps (AP) is a flowering perennial used as a traditional medicine and dietary supplement across East Asia. No study has yet assessed its effects on synaptic plasticity in hippocampus and much less in a model of ovarian hormone deficiency. We examined the influence of chronic oral AP ethanol extract treatment in ovariectomized rats on the induction of long-term depression in a representative synapse (CA3-CA1) of the hippocampus. Ovariectomized rats demonstrated lower trabecular mean bone mineral densities than sham, validating the establishment of pathology. Against this background of pathology, AP-treated ovariectomized rats exhibited attenuated long-term depression (LTD) in CA1 relative to water-treated controls as measured by increased field excitatory post-synaptic potentials (fEPSP) activation averages over the post-stimulation period. While pathological significance of long-term depression (LTD) in ovariectomized rats is conflicting, that AP treatment significantly affected its induction offers justification for further study of its influences on plasticity and its related disorders Artemisia princeps (AP) is a flowering perennial used as a traditional medicine and dietary supplement across East Asia. No study has yet assessed its effects on synaptic plasticity in hippocampus and much less in a model of ovarian hormone deficiency. We examined the influence of chronic oral AP ethanol extract treatment in ovariectomized rats on the induction of long-term depression in a representative synapse (CA3-CA1) of the hippocampus. Ovariectomized rats demonstrated lower trabecular mean bone mineral densities than sham, validating the establishment of pathology. Against this background of pathology, AP-treated ovariectomized rats exhibited attenuated long-term depression (LTD) in CA1 relative to water-treated controls as measured by increased field excitatory post-synaptic potentials (fEPSP) activation averages over the post-stimulation period. While pathological significance of long-term depression (LTD) in ovariectomized rats is conflicting, that AP treatment significantly affected its induction offers justification for further study of its influences on plasticity and its related disorders.