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천연과즙이 알로에(Aloe vera. L)조직의 callus 배양에 미치는 효과 검정
오명원,권오민,배동녁,한구태,김대환,도건엽,이지홍,장경호,김영철,김완동,권정호,이인순,문혜연 대구대학교 산업기술연구소 2012 産業技術硏究 Vol.23 No.1
We studied the effects of natural juices as grape and apple juice on callus formation of Aloe vera. L. The condition medium for callus differentiation was made MS medium with 0.2 ㎎/ℓ IAA, 0.3 ㎎/ℓ kinetin and 100 ㎎/ℓ grape or/and apple juice. The callus tissue was induced on this medium for 21 days, but callus formation was not found. However, cut aloe tissue released an extracellular material into media after 7 days as the culture progressed. The condition medium with grape juice brought to decrease cellular metabolism due to synthesis phenol and flavonoid which may resulted by necrosis of tissues like browning reaction. The an extracellular material and aloe tissue were extracted by as a solvent from medium, cultured aloe tissue and natural aloe tissue and those extracts were analysed for the determinate the concentration of phenol, flavonoid and vitamin C content. In the result, the control medium without addition of natural juice had increased of phenol compound and flavonoid content. The condition medium with addition of apple juice had higher content of flavonoid and vitamin C compared with other extracts. As a result, a composition of the apple juice stimulate metabolism of functional material synthesis.