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한국인 두경부암 환자에서 CYP1A1 및 GSTM1 유전자 다형성 분석에 의한 유전적 감수성에 대한 연구
김현준(Hyun Jun Kim),채현기(Hyun Ki Chae),태경(Kyung Tae),공구(Gu Kong) 한국환경성돌연변이발암원학회 2000 한국환경성돌연변이·발암원학회지 Vol.20 No.1
Genetic polymorphisms of metabolizing enzymes to chemical carcinogens have been recognized as a major important host factors in human cancers. To determine the frequencies of genotypes of CYP1A1 and GSTM1 metabolizing enzymes in healthy controls and head and neck cancer patients in Korean and to identify the relative high risk genotypes of these metabolizing enzymes to head and neck cancer, we have analyzed 133 head and neck cancer patients and corresponding healthy controls matched in age and sex using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). In analysis of CYP1A1, the Val/Val genotype of exon 7 polymorphism and m2/m2 genotype of Msp 1 polymorphism were associated with higher relative risks to head and neck cancers (Odds ratio : 2.34, 95% CI : 0.79-6.96 and 1.27, 95% CI : 0.59-2.73,<br/> respectively). In combined genotyping of CYP1A1 and GSTM1 enzymes polymorphisms, the patients with Val/Val and GSTM1(-), and m1/m2l and GSTM1(-) combined genotypes had higher relative risks than the patients with each base genotype of combined genotypes (Odds ratio : 4.57, 95% CI : 0.51-41.25 and 1.65, 95% CI : 0.73-3.77, respectively). These results suggest the combined genotype of Val/Val and GSTM1(-) was more susceptible to head and neck cancers in Korean and genotyping of metabolizing enzymes could be useful for predicting individual genetic susceptibility and screening the high risk subpopulation to head and neck cancer in Korean.
Ki Ok. Shin(신기옥),Hyun Jun. Kim(김현준),Sung Hwun. Kang(강성훈) 한국생명과학회 2008 생명과학회지 Vol.18 No.3
본 연구의 목적은 1) 캡사이신(75 ㎎), 세사민(30 ㎎), 그리고 카르니틴(900 ㎎) 복합성분(CCSC)이 에너지 대사조절자로서 열발생 교감신경활동과 관련된 자율신경활동을 향상시키는지, 2) 본 연구의 대상자들의 β₃-AR 유전자 다양성이 자율신경활동에 영향을 주는지를 조사하였다. 7명의 대상자(24.7±1.8세)가 이 실험에 자발적으로 참여하였다. 심박수변이성 파워스펙트랄 분석에 의해 평가된 심장자율신경활동은 CCSC 섭취 전과 후 총 120분간 매 30분마다 5분간 측정하였으며, CCSC 또는 위약 (CON)그룹은 무작위로 대상자에서 섭취되었다. 본 연구의 결과에서, 총 대상자중, β3-AR 유전자CC타입을 가진 대상자는 없었다. 두 그룹간의 안정시 심박수에서는 유의한 차이가 없었다. 자율신경활동에서도 그룹간 차이는 없었으나, CCSC그룹에서 섭취전과 섭취 후 30분에서 총자율신경활동(TOTAL power), 부교감신경활동(PNS power), 그리고 교감·부교감 신경활동지수에서 유의한 차이를 보였다(p<0.05, respectively). 이상의 결과로서, 비록 각각의 성분들은 지방분해와 관련이 있다 할지라도CCSC의 섭취는 열생산 교감신경 자극에 영향을 주지 않았으며, 오히려 양 교감·부교감신경활동의 향상과 관련이 있다는것을 시사하였다. 따라서 미래의 연구에서는 ANS 활동에 영향을 주는 유전적 다양성뿐만 아니라 운동능력 향상 보조물 그리고/또는 지방분해 효과를 위한 더 많은 복합 영양 성분이 연구되어야 할 것이다. We investigated whether 1) the combined capsaicin (75 ㎎), sesamin (30 ㎎), and L-carnitine (900 ㎎) (CCSC) ingestion enhances autonomic nervous system (ANS) activities including thermogenic sympathetic activity as energy metabolic modulator, 2) β₃-AR polymorphism of each subject influences with ANS activity. Seven healthy males (22.0±0.5 yr) volunteered for this study. The cardiac autonomic nervous activities evaluated by means of heart rate variability of power spectral analysis were continuously measured during 5 min every 30 min for total 120 min resting condition with CCSC or placebo oral administration chosen at random. The results indicated that, there are not Arp/Arg?⁴ variants of the β₃-AR genotypes in our subjects. There were not also significant differences in heart rate during rest between both trials. The difference of ANS activity did not reach the statistical significance between both trials. However, the significant improvement showed TOTAL power, HF component, and the indices of SNS and PNS activities before and at 30 min after CCSC ingestion (p<0.05, respectively). In conclusions, although each component of combined CCSC is associated with lipolysis and/or fat oxidation, the combined CCSC consumption is not influenced in stimulation of thermogenic sympathetic activity as modulator of energy metabolism. In rather, our results suggested that CCSC ingestion improves the balance of both SNS and PNS activities. Therefore, it will be considered many combined nutrient components for ergogenic and/or lipolysis effects as well as genetic variants affecting ANS activity in further studies.