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니코틴이 치은섬유아세포의 세포주기 조절 단백질 발현에 미치는 영향
김탁,김재호,피성희,김은철,유용욱,유형근,신형식,Kim, Tak,Kim, Jae-ho,Pi, Sung-Hee,Kim, Eun-Cheol,You, Yong-Ouk,You, Hyung-Keun,Shin, Hyung-Shik 대한치주과학회 2001 Journal of Periodontal & Implant Science Vol.31 No.3
Normal gingival fibroblasts functioning is fundamental for the maintenance of periodontal connective tissue as well as wound healing. Nicotine have been found to affect DNA synthesis and cell proliferation, which appear to depend on the type of cells. This in vitro study was done to determine the effects of nicotine, a major component of tobacco, on cell proliferation, viability, activity, cell cycle distribution, and expression of cell cycle regulatory proteins in human gingival fibroblasts. Nicotine has been tested for 2 days or 4 days in 5 different concentrations; $0.1{\mu}g/ml$; $1{\mu}g/ml$; $10{\mu}g/ml$; $100{\mu}g/ml$; $1000{\mu}g/ml$. To assess cell proliferation and viability, viable and non-viable cells were counted by hemocytometer; to evaluate cellular activity, MTT assay was employed; to analyze cell cycle distribution, fluorescent propidium iodide-DNA complex were measured using fluorocytometer; to determine the expression of cell cycle regulatory proteins, western blot analysis was performed. After 2 days and 4 days incubation respectively, at concentrations of $1{\mu}g/ml$ - $1000{\mu}g/ml$, nicotine significantly inhibited proliferation comparing to non-supplemented controls. The cell viability was significantly decreased after 2 days and 4 days at concentrations of $1{\mu}g/ml$ - $1000{\mu}g/ml$ and at $10{\mu}g/ml$ - $1000{\mu}g/ml$ respectively. After 2 days and 4 days, the cellular activity was significantly decreased at concentrations of $10{\mu}g/ml$ - $1000{\mu}g/ml$. Treatment with $100{\mu}g/ml$ nicotine for 48 hours caused an increase in the proportion of G1-phase cells (from 46.41% to 53.46%) and a decrease in the proportion of S-phase cells (from 17.80% to 14.27%). The levels of cyclin $D_1$ and CDK 4 proteins in nicotine-treated fibroblasts were lower than that of controls, whereas the levels of p16 and pRB were higher than that of controls. These results suggest that the decrease of cell proliferation and lengthened Gap phases (G1) by nicotine may due to the increased expression of p16 and pRB as well as decreased expression of cyclin $D_1$ and CDK 4 in human gingival fibroblasts.
Etiology, Characteristics, and Outcomes of Community-Onset Pyomyositis in Korea: A Multicenter Study
김탁,Park Seong Yeon,Kwak Yee Gyung,정지원,Kim Min-Chul,최성호,Yu Shi Nae,Hong Hyo-Lim,Kim Yong Kyun,Park Se Yoon,Song Eun Hee,Park Ki-Ho,조오현,Choi Sang-Ho,The Korean SSTI Study Group 대한감염학회 2021 Infection and Chemotherapy Vol.53 No.1
Background: Pyomyositis (PM) is a serious soft tissue infection and despite its clinical importance, previous studies have not been able to fully determine the clinical characteristics and microbial epidemiology of PM in Korea, which we therefore aimed to investigate. Materials and Methods: We retrospectively identified 140 adult patients diagnosed with PM from 13 general hospitals between January 2012 and December 2015. We analyzed the clinical and microbial characteristics of community-onset PM and compared them with communityacquired (CA) and healthcare-associated (HCA) PM. Results: One hundred eleven organisms were isolated from 96 (68.6%) patients with PM. Staphylococcus aureus (38 patients) was the most common pathogen, followed by streptococci (24 patients), and enteric Gram-negative organisms (27 patients). Methicillin-resistant S. aureus (MRSA) was identified in four (2.9%) patients and in-hospital mortality reached 8.6% (12/140). Enterococci isolates were identified in the HCA PM subgroup only The proportion of MRSA isolates was not comparable between CA and HCA PM subgroups. In the 83 patients with PM infected by monomicrobial pathogens, isolates of Gram-negative organisms were more commonly found in HCA PM subgroup than in CA PM subgroup (47.6% [10/21] of patients with HCA PM vs. 20.7% [12/58] of patients with CA PM; P = 0.01). Conclusion: Gram-positive cocci such as S. aureus and streptococci were dominant etiologies in community-onset PM, whereas MRSA appears to an uncommon causative organism of PM in Korea. Enteric Gram-negative organisms should also be considered as major etiologies, especially in HCA PM patient population in Korea.
강의 동영상의 효과적인 브라우징을 위한 강인한 슬라이드 추출 기법
김탁은(Tak-Eun Kim),임선규(Seon Kyu Lim),김명호(Myoung Ho Kim) 한국정보과학회 2012 정보과학회 컴퓨팅의 실제 논문지 Vol.18 No.1
비디오 브라우징 기법은 동영상의 주요 장면들을 추출하여 프리뷰로 보여줌으로써, 사용자가 동영상의 주요 장면에 쉽고 빠르게 접근할 수 있도록 한다. 강의 동영상은 일반 동영상과는 달리 매우 정적이므로, 기존 동영상 브라우징 기법은 강의 동영상에 적용하기에 알맞지 않다. 본 연구에서는 강의 동영상에 주로 슬라이드가 나온다는 점에 착안하여, 영상에서 슬라이드 이미지를 추출하여 이를 주요 장면으로 지정하는 방법에 대해 연구하고, 강의 동영상 브라우징 인터페이스를 구현하였다. The video browsing method that extracts key frames and utilizes them as previews makes users possible to access key frames easily and directly. Compare to videos such as movies, lecture videos has relatively low scene changes. Therefore, the existing video browsing methods, which utilize a rapid change of frames for key frame extractions, are unsuitable for lecture videos. In this paper, we propose a lecture video browsing method that extracts lecture slides from a lecture video, and utilizes them as previews. The previews are combined with a video player to improve the accessibility of contents in a lecture video.
김탁은(Tak-Eun Kim),김명호(Myoung Ho Kim) 한국정보과학회 2010 한국정보과학회 학술발표논문집 Vol.37 No.1C
최근 시맨틱 웹에 대한 많은 관심이 집중되고 있다. 그러나 시맨틱 웹 기술의 핵심이 되는 온톨로지 개발의 어려움과, 정형화된 개발 방법의 부재로 인해 일반 개발자들이 시맨틱 웹 응용프로그램을 개발하는 데에 많은 어려움을 겪고 있다. 본 연구에서는 온톨로지와 관련 기능들을 컴포넌트화 하고, 컴포넌트들을 결합하여 응용프로그램을 쉽고 빠르게 구현하기 위한 프레임워크를 제안한다. 이는 복잡한 시맨틱 웹 기술을 숨김으로써, 일반 개발자들이 시맨틱 웹에 대한 깊은 이해 없이도 시맨틱 웹 응용프로그램을 쉽고 빠르게 구현할 수 있도록 한다.
체외수정을 위한 과배란유도 주기에서 내인성 LH Surge의 발생에 관한 연구
김탁,김선행,구병삼,주갑순,Kim, Tak,Kim, Sun-Haeng,Ku, Pyong-Sham,Joo, Kap-Soon 대한생식의학회 1989 Clinical and Experimental Reproductive Medicine Vol.16 No.1
71 cycles of 67 women were treated for superovulation induction in our IVF & ET program from May to September in 1988. Endogenous LH surges were occurred in 21 cycles out of 71 cycles. And then, we selected 50 cycles without endogenous LH surge treated in the same period as control group. We compared egg recovery rate, egg maturity, fertilization rate, cleavage rate and pregnancy rate of study group with those of control group. We were able to detect more than 90% of endogenous LH surge by commencing daily LH monitoring on MCD 9. The egg recovery rate, egg maturity, fertilization rate, and pregnancy rate of the study group were not statistically different from those of the control group. Significantly lower cleavage rate was seen in the study group compared with that of control group. Above results suggested that the cycles with endogenous LH surge do not have to be abandoned and can be treated continuously to achieve successful pregnancy.