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중공 및 중실에 따른 철근콘크리트 부재의 비틀림 최대내력평가
김민원 ( Kim¸ Min-won ),김준구 ( Kim¸ Jun-gu ),이가영 ( Lee¸ Ga-yeong ),김형국 ( Kim¸ Hyeong-gook ),김길희 ( Kim¸ Kil-hee ) 한국구조물진단유지관리공학회 2023 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.27 No.2
이 연구에서는 최근 늘어나는 중공 단면 부재의 비틀림을 평가하기 위하여 중공(Hollow) 및 중실(Solid)을 변수로 RC 부재에 대한 비틀림 최대 내력(T<sub>n</sub>)과 에너지 소산 능력을 비교하였다. 비틀림 최대 내력은 중실 실험체가 중공 실험체보다 약 4.5% 높게 나타났으며, 에너지 소산 능력은 중실 실험체가 중공 실험체보다 약 3% 높게 나타났다. 이를 통해 중공형태의 부재는 비틀림 최대 내력과 에너지 소산 능력에 대한 영향이 미미한 것을 확인하였다.
홍대희,김우동,김태형,민원,Hong, Daehie,Kim, Woo-Dong,Kim, Tae-Hyung,Min, Won 대한기계학회 2002 大韓機械學會論文集B Vol.26 No.1
The tunnels built in recent years are equipped with traffic counters and pollution sensors (mostly, CO and Vl sensors). Utilizing these built-in sensors, it is possible to develop an algorithm to estimate the amount of pollutants exhausted from the each class of cars passing through the tunnel. These estimated data can be effectively utilized not only for ventilation control but also for designing ventilation facilities. The diffusion of pollutants in a tunnel can be described with one-dimensional diffusion-convection equation. This equation is approximated with interpolation functions and weighted residual method converting to adequate form for standard state estimate algorithms. With this converted equations, a least square optimization based algorithm is developed, whose outputs are the estimated amounts of pollutants emitted from each class of cars. In order to verify the feasibility of the developed algorithms, simulations are performed with the real data acquisitioned from the Tunnae tunnel located in Young-Dong highway in Korea.
Amoxacillin의 경구투여에 따른 양식 어류(넙치, 조피볼락, 참돔)의 근육조직내 잔류량의 변화
정희식,김석,민원기,이후장,Chung, Hee-Sik,Kim, Suk,Min, Won-Gi,Lee, Hu-Jang 한국식품위생안전성학회 2006 한국식품위생안전성학회지 Vol.21 No.4
The residue depletion of amoxicillin was investigated in the olive flounder (Paralichthys olivaceus), rockfish (Sebastes schlegeli), and red sea bream (Pagrus major) after 7 days treatment with medicated feed at a dose of 400 mg/kg bw/day. Fishes were sampled for muscle on 1st, 2nd, 3rd, 4th, and 5th day after treatment. Amoxicillin concentrations were determined by high performance liquid chromatography with fluorescence detector. The recovery rates of amoxicillin in muscle samples ranged 84.3-101.3% and 75.0-91.5% for the concentration of 0.05 mg/kg and 0.1 mg/kg, respectively. Amoxicillin concentrations detected on 1st day after treatment were 0.137, 0.131, and 0.172 mg/kg in the muscle of olive flounder, rockfish, and red sea bream, respectively. After a withdrawal of 3 days, muscle concentrations were 0.012, 0.010, and 0.017 mg/kg in the olive flounder, rockfish, and red sea bream, respectively. Amoxicillin was not detectable in muscle samples on 4 days following withdrawal of the medicated feed. From results of the present study, a withdrawal period of amoxicillin is proposed on 4 days after 7 days treatment with medicated feed at a dose of 400 mg/kg bw/day to avoid the presence of excessive residues of the edible muscles of olive flounder, rockfish, and red sea bream. Amoxicillin을 넙치, 조피볼락 그리고 참돔에 각각 400 mg/kg bw/day의 용량으로 7일 동안 사료와 혼합하여 경구 투여한 다음, 휴약기간 동안 근육조직 내 잔류 분포를 조사하였다. 실험어는 해수 중에서 일정한 크기의 케이지에 일반 상업용 사료를 주어 사육하였고, 실험에 사용하기에 앞서 15일 동안 환경에 적응시켰다. 약제 투여 후, 근육시료는 1, 2, 3, 4, 그리고 5일에 각각의 실험어를 대상으로 채취하였다. Amoxicillin의 잔류분석은 형광검출기를 부착한 고속액체크로마토그래피를 이용하여 분석하였다. Amoxicillin의 회수율은, 0.05mg/kg의 농도에서 84.3-101.3%, 0.1mg/kg의 농도에서는 75.0-91.5%를 보였다. 투약 후 1일에는, 참돔의 근육 중 amoxicillin의 잔류농도가 넙치와 조피볼락의 근육 중 잔류농도에 비하여 높았으나 통계적 유의성은 없었으며, 투약 후 4일에는, 모든 근육 시료에서 amoxicillin이 검출되지 않았다. 이상의 결과로부터, amoxicillin의 사료혼합을 통한 경구 투여는 넙치, 조피볼락 그리고 참돔의 근육 중에서 안전휴약기간(7일) 보다도 체래 소실이 빨리 일어나는 것으로 추정되는 바, 안전휴약기간을 준수한다면 amoxicillin의 어류 근육 조직 내 잔류로부터 안전할 것으로 사료된다.
원저 : 삼백초 ethanol 추출물의 RAW 264.7세포 내 살모넬라균 감염에 대한 항균 효과
이진주 ( Jin Ju Lee ),김동혁 ( Dong Hyeok Kim ),임정주 ( Jeong Ju Lim ),김대근 ( Dae Geun Kim ),김곤섭 ( Gon Sub Kim ),민원기 ( Won Gi Min ),이후장 ( Hu Jang Lee ),장홍희 ( Hong Hee Chang ),김석 ( Suk Kim ) 한국수의공중보건학회 2010 예방수의학회지 Vol.34 No.4
Salmonellosis is the commonest zoonosis worldwide that generally causes enterocolitis and foodborne poisoning which represents a considerable public health burden. Salmonella spp. are potential enteric pathogens and intracellularly replicates in host cells resulting in chronic infections. The medical treatments for salmonellosis have been difficult yet and had a serious problem including the increasing emergence of antibiotic resistance. The present report was designated to investigate the antibacterial effects of Saururus chinensis Baill ethanol extract (SCEE) on pure culture and infection with Salmonella enterica serovar Typhimurium (S. typhimurium) in murine derived macrophage RAW 264.7 cells. In determination of antibacterial activity of SCEE against S. typhimurium, bacterial viability was markedly decreased compared to the control. Also, SCEE significantly induced morphological change (p<0.05) of RAW 264.7 cells. In infection assay of S. typhimurium in RAW 264.7 cells pretreated with 100㎍/㎖of SCEE, which is a non-cytotoxic concentration, bacterial uptake ability of macrophage was increased corresponding with morphological change, whereas bacterial survival rates within macrophage were markedly reduced compared with untreated control. Furthermore, nitric oxide (NO) production in SCEE-treated cells was slightly increased until 2 h but showed a tendency of decrease after 4 h until 24 h post infection compared with untreated control with S. typhimurium infection. Taken together, these findings demonstrated that SCEE has the antibacterial activity for S. typhimurium and the protective effects against S. typhimurium infection through activating murine macrophage independent on NO, suggesting that SCEE may be beneficial on the disease caused by intracellularly replicating pathogens as a safe alternatives of conventional chemotherapies.
고압멸균 처리시간에 따른 닭 체내 조류인플루엔자와 뉴캐슬병 바이러스의 멸균효과
이진주 ( Jin Ju Lee ),김동혁 ( Dong Hyeok Kim ),임정주 ( Jeong Ju Lim ),김대근 ( Dae Geun Kim ),민원기 ( Won Gi Min ),이후장 ( Hu Jang Lee ),장홍희 ( Hong Hee Chang ),김석 ( Suk Kim ) 한국예방수의학회(구 한국수의공중보건학회) 2011 예방수의학회지 Vol.35 No.4
Avian influenza (AI) and Newcastle disease (ND) distress a variety of avian species, especially domestic poultry. Severe syndromes are caused by highly virulent specific virus strains termed highly pathogenic AI and velogenic ND viruses, which are potential agrobioterrorism agents. This outbreak emphasizes the need for continuing cooperation between public health and veterinary medical communities in controlling AI and ND when it has a zoonotic potential. Up to date, the stamping out and burying system were applied for controlling methods against these highly infectious diseases in the ordinary way, however these methods had many environmental problems, including leachat and effluvium. Thus, we assessed that sterilization effect of AI and ND virus dependent on several treatment conditions, such as autoclaving time and cutting types of chicken. As a result, we found that the cutting type of chicken meat revealed a reduced HA titer (20) against both of AI and ND virus after 10 min of autoclaving, while whole chicken showed same titer after 30 and 60 min. Therefore, we propose that the conditions of treatment on infected chicken should be developed for convenient, affordable, and effective prevention methods against for AI and ND.
복합하중을 받는 RC 부재의 휨모멘트비에 따른 비틀림 거동
이가영 ( Lee Ga-yeong ),김준구 ( Kim Jun-gu ),김민원 ( Kim Min-won ),김형국 ( Kim Hyeong-gook ),김길희 ( Kim Kil-hee ) 한국구조물진단유지관리공학회 2023 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.27 No.1
이 연구에서는 휨모멘트비를 변수로 하여 복합하중(축력, 휨, 전단, 비틀림)을 받는 RC 부재의 비틀림 거동을 비교 및 평가하였다. 순수비틀림 실험체와 비교하여 0.3My, 0.9My 실험체의 최대내력 (Tn)이 약 2-8% 감소하였으며, ACI에 따른 산정 값과의 비교에서 모든 실험체가 40% 이상 증가하여 과소평가하는 것을 확인하였다. 이에 설계기준의 비틀림 산정식에서 복합하중이 비틀림 거동에 미치는 영향을 고려할 필요가 있다고 판단된다.