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육우 수정란의 간역동결 및 융해방법에 관한 연구 3 . Sucrose 첨가한 glycerol 희석액에 간역동결 및 융해방법이 가토수정란 생존율에 미치는 영향
김중계(J . K . Kim),김철균(C . K . Kim),강만종(M . J . Kang),김영훈(Y . H . Kim),강민수(M . S . Kang) 한국축산학회 1988 한국축산학회지 Vol.30 No.10
This study was conducted to simplify the method for glycerol addition (a single step; 1S and 3-step addition; 3S) and diluent with 10% sucrose, and the method for freezing. Survival rates of rabbit embryos cultured after frozen in a liquid nitrogen container with different methods and thawed at different temperatures were determined. The summarized results are the following. 1. The embryo survival rates when a cryoprotectant diluent containing 10% sucrose were 75.3% (1S) and 69.8% (3S) (p $lt;0.05). 2. The survival rate of embryos cultured after thawed in a water bath at 38℃ or 5℃ was 75.5 or 69.6%, respectively (p $lt;0.05). 3. The survival rate of embryos thawed and cultured after the embryos at room temperature were frozen in a liquid nitrogen down to -7℃ at the rate of 1℃/min and then 0.3℃ (1 F), 3∼5℃(2F) 15℃/min (3F) or frozen in the gas phase 2 - 5 mm above the liquid nitrogen for 5 min and then inside the liquid nitrogen (4F) was 78, 71, 62 or 63%, respectively (p $lt;0.05). 4. The above results indicate that 1S (vs 3S), thawing at 38℃ (vs 5℃) and 1F (vs the others) showed higher embryo survival rates. However, the rapid freezing methods (2F, 3F and 4F) was considered relatively good.
육우 수정란의 간역동결 및 융해방법에 관한 연구 4 . 내동제에 sucrose 첨가시 간역동결과 식빙방법이 FDA test 에 의한 가토수정란 생존율에 미치는 영향
김중계(J . K . Kim),김철균(C . K . Kim),강만종(M . J . Kang),장덕지(D . J . Chang),김승호(S . H . Kim) 한국축산학회 1988 한국축산학회지 Vol.30 No.10
Effects of simplified procedures of freezing in a liquid nitrogen container and seeding on rabbit embryo survival rates were determined using the FDA test. The results found are summarized as follows: 1. When a single-step addition and removal of a cryoprotectant and a diluent, both containing 10% sucrose was used, seeding with a pincette resulted in a lower FDA test score (2.8) than that without seeding (3.2), but there was no significant differences. 2. Test score was 3.1, 3.2, 3.3 or 3.0 for embryos frozen in a liquid nitrogen container at the rate of 0.3℃, 3^5℃, 15℃/min or frozen in the gas phase 2∼5 mm above the liquid nitrogen and then inserted them into the liquid nitrogen, respectively. No significant differences were found between the freezing methods. 3. The FDA test was considered to be a simple and inexpensive procedure, as compared to the conventional test of embryo survival after culturing in CO₂ incubator.