육우 수정란의 간역동결 및 융해방법에 관한 연구 4 . 내동제에 sucrose 첨가시 간역동결과 식빙방법이 FDA test 에 의한 가토수정란 생존율에 미치는 영향 = Studies of Simplified Procedures for Freezing and Thawing of Bovine Embryos 4 . Effects of simplified procedures of freezing and seeding using a cryoprotectant containing sucrose on the rabbit embryo survival rate

다국어 초록 (Multilingual Abstract)

Effects of simplified procedures of freezing in a liquid nitrogen container and seeding on rabbit embryo survival rates were determined using the FDA test. The results found are summarized as follows: 1. When a single-step addition and removal of a cryoprotectant and a diluent, both containing 10% sucrose was used, seeding with a pincette resulted in a lower FDA test score (2.8) than that without seeding (3.2), but there was no significant differences. 2. Test score was 3.1, 3.2, 3.3 or 3.0 for embryos frozen in a liquid nitrogen container at the rate of 0.3℃, 3^5℃, 15℃/min or frozen in the gas phase 2∼5 mm above the liquid nitrogen and then inserted them into the liquid nitrogen, respectively. No significant differences were found between the freezing methods. 3. The FDA test was considered to be a simple and inexpensive procedure, as compared to the conventional test of embryo survival after culturing in CO₂ incubator.