한국 폐경여성에서 비타민 D 수용체 유전자내 Poly (A) 다형성과 골밀도의 연관성

김정구 ( Jung Gu Kim ),권재희 ( Jae Hee Kwon ),김석현 ( Seok Hyun Kim ),최영민 ( Young Min Choi ),문신용 ( Shin Yong Moon ),이진용 ( Jin Yong Lee ) 대한산부인과학회 2003 Obstetrics & Gynecology Science Vol.46 No.4

목적 : 한국 폐경여성에서 VDR 유전자내 poly (A) 다형성양상을 알아보고 이런 양상과 골밀도 사이에 연관성이 있는가를 규명하고자 하였다. 연구 방법 : 자연 폐경여성 417명에서 VDR 유전자내 poly (A) 다형성양상을 urea-polyacrylamide 겔 전기영동, GeneScan, 염기서열분석으로 분석하고 혈청 CTX, osteocalcin, BAP, 1,25 (OH)2 비타민 D3 농도를 효소면역법과 면역측정법으로, 요추 및 대퇴 근위 Objective : To investigate a poly (A) microsatellite in vitamin D receptor (VDR) gene and to evaluate its relationship with bone mineral density (BMD) in postmenopausal Korean women. Methods : The poly (A) polymorphism was analyzed in 417 postmenopausal K

유아 전래동요 활동 효과 연구에 관한 메타분석

황성온 ( Hwang Sung On ),권재희 ( Kwon Jae Hee ) 한국유아교육학회 2018 유아교육연구 Vol.38 No.2

본 연구의 목적은 메타분석을 활용하여 유아 전래동요 활동의 효과를 총체적으로 분석하고, 이를 바탕으로 유아 전래동요 프로그램 개발을 위한 기초자료를 제시하는데 있다. 이를 위하여 2000년부터 2017년까지의 국내 학위논문 및 학술지 게재 논문 27편을 대상으로 랜덤효과모형을 적용하여 효과크기를 분석 한 결과, 전체 평균 효과크기는 1.19로 유아 전래동요 활동이 유아에게 미치는 효과가 매우 큰 것으로 나타났다. 종속변인에 따른 효과크기 차이 분석 결과, 통계적으로 유의미한 차이는 나타나지 않았으나 사회성발달에서 가장 큰 효과크기를 보였다. 조절변인별로 효과크기를 살펴본 결과, 실험집단 인원수가 26명 이상, 만 4세와 5세의 혼합연령 집단으로 구성, 실험처치 기간은 7-9주, 실험처치 횟수는 31회 이상, 언어활동과 연계된 전래동요 활동에서 효과크기가 가장 컸으며, 실험처치 기간과 횟수에 따라 통계적으로 유의미한 효과크기 차이가 나타났다. The purpose of this study was to analyze the effects of traditional children’s song activities by using Meta-analysis and to provide basic data for developing programs for those activities. For this purpose, the effect size of 27 selected studies published between 2000 and 2017 were computed while applying random effect model. The result of analysis shows average effect size of all traditional children’s song activities was 1.19 which means traditional children’s song activities for young children are highly effective. Though the difference of effect size depending on dependent variables was not statistically significant, social development was the highest scored variable in effect size. Furthermore, there were statistically significant differences depending on the duration and total number of treatments. The effect was higher when the size of experimental group member was ‘over 26’, the age of experimental group member was a mixture of 4 and 5 years olds, duration of experimentation was 7-9 weeks, the number of experimentation was more than 31 times, and traditional children’s song activities were associated with language activities.

미술치료를 통한 자기통제: ADHD 아동을 위한 소조활동 중심으로

최은영 ( Eun Young Choi ),권재희 ( Jae Hee Kwon ),김수진 ( Soo Jin Kim ),박선욱 ( Sun Wook Park ),소은실 ( Eun Sil So ),이은혜 ( Eun Hye Lee ),장현정 ( Hyun Jung Jang ) 한국정서.행동장애아교육학회(구.한국정서학습장애아교육학회) 2006 정서 학습장애아교육 학술발표회 Vol.14 No.2

Inattention, impulsiveness and hyperactivity is the core symptoms of ADHD. If problem in childhood were not solved, low self-esteem could appear in adolescent period and it can last in adulthood. Also they could become substance abuse, conduct disorder and antisocial personality disorder. For this reason, it is very necessary that intervention for ADHD. ADHD children`s core symptom is lack of self-control that could stop or control their behavior. They didn`t know how could stop their impulsive thought or behavior, and they feel regret for their impulsiveness. When self-control is well done, they do dangerous behavior less, stand their anger, perform complex mental task well, control immediate satisfaction, rather than it is not(Barkley, 2005). For this reason, self-control is very necessary skill to ADHD. Recently, there is report that art therapy through imagery, specially visual imagery could maximize self-control. And media is one of the major factor of art therapy. Hence, in this study, we discussed about significance of art therapy for self-control in ADHD. And we investigated possibility of use about clay that is important media.

한국 폐경여성에서 비타민 D 수용체 유전자 다형성과 골밀도의 연관성

김정구 ( Jung Gu Kim ),권재희 ( Jae Hee Kwon ),김석현 ( Seok Hyun Kim ),최영민 ( Young Min Choi ),문신용 ( Shin Yong Moon ),이진용 ( Jin Yon Lee ) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.11

목적: 한국 폐경여성에서 VDR 유전자 다형성 양상과 골밀도 사이에 연관성이 있는가와 이러한 VDR 유전자 다형성이 혈청 1,25 (OH)2 비타민 D3와 골교체의 생화학적 인자 농도에 영향을 미치는가를 알아보고자 하였다. 연구 방법: 자연폐경여성 433명에서 VDR 유전자내 BsmI, TaqI, ApaI, FokI 제한효소부위의 다형성양상를 PCR과 제한효소 처리 후 나타나는 전기영동 양상으로 분석하고 혈청 CTX, osteocalcin, BAP, 1 Objective: To investigate the relationship between vitamin D receptor (VDR) gene polymorphisms and bone mineral density (BMD) in postmenopausal Korean women, and to evaluate if VDR gene polymorphisms are associated with serum levels of 1,25 (OH)2 vitamin

동결 해동한 포배 이식에 있어서 동결시기가 임신결과에 미치는 영향

김현정,김충현,이중엽,권재희,황도영,김기철,Kim, Hyun-Jung,Kim, Chung-Hyon,Lee, Joong-Yeup,Kwon, Jae-Hee,Hwang, Do-Yeong,Kim, Ki-Chul 대한생식의학회 2010 Clinical and Experimental Reproductive Medicine Vol.37 No.1

목 적: 배양 환경과 동결 기술이 발달함에 따라 동결 포배의 해동-이식의 빈도가 증가하고 있으며, 신선 주기와 마찬가지로 해동-이식 주기에서도 질 좋은 배아를 선별하는 것은 임신 성공 여부를 결정하는 아주 중요한 과제이다. 본 연구는 동결 당시 포배로의 발달 속도가 임신 결과에 미치는 영향을 알아보기 위하여, 수정 후 5일 및 6일째 동결한 포배의 해동-이식 후 임신율을 비교 분석하였다. 연구방법: 2006년 1월부터 12월까지 5일째 또는 6일째 동결한 포배를 해동하여 2007년 6월까지 융해 이식한 87명, 93주기를 대상으로 하였다. 동결법은 ethylene glycol과 DMSO를 이용한 유리화 동결법을 이용하였으며, 팽창 포배는 인위적인 수축을 시행 후 동결하였다. 해동 과정은 이식 전날 시행하여 15~18시간 배양액에서 배양 후 재팽창 여부를 확인하였다. 결 과: 5일째 동결한 포배를 해동-이식한 52주기와 6일째 동결한 포배를 해동-이식한 41주기에서 환자의 나이, 이식한 배아의 수, 해동 배아의 생존율 등 임신 결과에 영향을 미칠만한 요인들의 차이는 없었다. 그러나 생화학적 임신율, 임상적 임신율, 진행 임신율, 착상율 등은 5일째 동결한 포배를 해동-이식한 주기에서 높게 나타났다. 결 론: 5일째 동결한 포배를 해동-이식했을 때의 임신율은 6일째 동결한 포배를 해동-이식했을 때의 임신율보다 2배 이상 높았으며, 이는 신선 주기와 마찬가지로 해동-이식 주기에서도 동결 전 배아의 발달 속도의 차이를 임신 성공 예측의 중요한 지표로 사용할 수 있음을 시사한다. Objectives: Likewise fresh cycle, it is also important to select right blastocysts for transfer in purpose of improving the pregnancy and implantation rates in frozen-thawed embryo transfer (ET) cycles. To investigate the relationship between the developmental velocity at the time of cryopreservation and pregnancy rates, we compared pregnancy rates between the day 5 cryopreservation group and the day 6 cryopreservation group. Methods: Transfers of frozen-thawed blastocysts which had been cryopreserved by vitrification on day 5 or day 6 were performed between January 2006 and June 2007. Ethylene glycol, DMSO, and pull and cut straws were used for vitrification and artificial shrinkage was done in expanded blastocysts. Thawing was performed on the day before transfer and thawed blastocysts were cultured in for 15~18 hrs in Quinn's blastocyct media. Blastocyst survival was assessed before transfer and post-thaw survival was defined as >50% of cells remaining intact and blastocoele re-expansion by the time of transfer. Results: Transfers of thawed blastocyst had been cryopreserved on day 5 were 52 cycles and 41 transfer cycles were cryopreserved on day 6. Patient characteristics, the number of transferred embryos and the survival rate of thawed blastocysts were not different in each cryopreservation day. But the biochemical pregnancy, clinical pregnancy, ongoing pregnancy, and implantation rate were significantly high in transfer of frozen-thawed blastocyst which were cryopreserved on day 5. Conclusions: The clinical pregnancy and implantation rate of day-5 blastocyst showed significantly higher than those of day-6 blastocyst in frozen-ET cycles. This result indicated that developmental rate of blastocyst at cryopreservation time in frozen-thawed cycle is discriminative marker of pregnancy outcome as like in fresh cycle.

인간 정자의 정밀 형태 분석에 관한 연구

문신용(Shin Yong Moon),최영민(Young Min Choi),김석현(Seok Hyun Kim),오선경(Sun Kyung Oh),서창석(Chang Suk Suh),이진용(Jin Yong Lee),권재희(Jae Hee Kwon),지병철(Byeong Chul Jee),정병준(Byeong Jun Jung),김희선(Hee Sun Kim),류범용(Buom 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.12

N/A The proportion of male factor infertility due to quantitative and qualitative sperm disorders is approximately 50-60% in infertile couples. In IVF-ET, lower or failed fertilization of oocytes usually results from subnormal count of total motile sperms, but this may occur in infertile couples even with normal sperm count. It has been suggested that some functional defects in sperms are responsible for lower or failed fertilization. Routine semen analysis based on numerical background has limits for the assessment of fertilization capacity of sperm in infertile males, and the andrologic test for the prediction of fertilization capacity must be objective, repeatable, quick, economic, and easily applicable for the clinical settings. The purposes of this study were to develop the analysis method of strict morphology of sperm using the strict criteria as a simple, inexpensive and useful test of sperm fertilization capacity, to establish the normal fertile range and the cut-off value of strict morphology, and to evaluate the validity of strict morphology as a prognostic indicator of fertilization capacity in IVF-ET. In establishing the effectiveness of strict morphology of sperm, ROC curve was used. Among the various thresholds for the prediction of fertilizing ability, normal morphologic value 10.0 corresponding to the value with higher sensitivity and lesser false positive rates was determined as a cut-off value. Using this cut-off point, the sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of strict morphology for the prediction of fertilization capacity was 73.9%, 81.0%, 80.6%, and 72.7%, respectively. To evaluate the clinical validity of strict morphology as a prognostic indicator of fertilization capacity, this cut-off point was applied to 133 patients undergoing IVF-ET. For the prediction of fertilization rate >30% in IVF-ET, the sensitivity, specificity, PPV, and NPV was 77.3%, 77.8%, 87.2%, and 63.6%, respectively. In conclusion, the strict morphology of sperm is one of the most simple and useful test for the assessment of fertilization capacity of sperm and the prediction of IVF-ET outcomes in infertile couples.

체외수정시술시 임신의 예후인자로서 자궁내막내 및 자궁근층내 동맥의 질식 초음파검사에 관한 연구

문신용(Shin Yong Moon),최영민(Young Min Choi),김석현(Seok Hyun Kim),서창석(Chang Suk Suh),이진용(Jin Yong Lee),김정구(Jung Gu Kim),권재희(Jae Hee Kwon),지병철(Byung Chul Jee) 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.1

N/A Objective: This study was performed to assess whether uterine intraendometrial or intramyometrial arterial blood flow impedance in Doppler sonography is related to the outcomes of IVF-ET cycles. Methods: A total of 24 patients undergoing IVF-ET was recruited for the study. All patients received controlled ovarian hyperstimulation(COH) with GnRH agonist and gonadotropins. Doppler sonographic examinations were performed by using Combison 530(Medison) with 5.5 MHz transvaginal probe. Pulsatility index(PI) was evaluated for uterine, radial, and spiral arteries on the days of hCG administration, oocyte retrieval, and embryo transfer(ET). Serum E2 level was assayed on the hCG day. Results: The overall pregnancy rate after IVF-ET was 29.2%(7/24). There was no significant difference in PI of uterine artery between pregnant and nonpregnant groups, No pregnancy occurred in 4(16.7%) patients without intraendometrial or subendometrial blood flow. Compared with nonpregnant group, PI of radial artery on the hCG day and PI of spiral artery on the hCG and oocyte retrieval days were significantly lower in pregnant group. There were no significant correlations between Serum E level on the hCG day and Pl of any three arteries. Conclusion: This study suggests that the Doppler sonographic parameter, PI, of intraendometrial and intramyometrial arteries are more useful than that of uterine artery not only in predicting the success of IVF-ET, but also in assessing the endometrial receptivity.

폐경 여성의 골밀도 : 자연 폐경 군과 수술적 폐경 군간의 비교 a comparison between natural and surgically induced menopause

이용희,최영민,이진용,권재희 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.5

폐경 여성의 골밀도의 양상과 폐경 원인에 따른 차이를 비교하고자 1991년 2월부터 1999년 5월까지 서울대학교병원 산부인과 폐경기 클리닉을 방문한 폐경 여성들 중 40세 이후에 폐경이 발생하였으며 골밀도 측정 전에 호르몬 대체요법을 받지 않았던 1,508명의 여성을 대상으로 후향적 고찰을 통해 골밀도 검사결과와 임상양상을 조사, 분석하였다. 이들의 골밀도는 이중에너지 방사선흡수계측법(DXA, Lunar Co.)을 사용하여 측정하였다. 총 대상자의 평균 연령은 53.7±5.7세였고 평균 골밀도는 요추 부위가 0.996±0.173 g/㎠, 대퇴경부가 0.821±0.133 g/㎠ 였으며 이 중 44.1%(665/1,508)가 골감소증에 해당하였고 14.7%(222/1,508)가 이미 골다공증에 이환 되어 있었다. 골다공증의 유병율은 40대에 5.2%이었고 50대에는 15.6%, 60대 32.5% 그리고 70대에 45.5%로 연령이 증가함에 따라 유의하게 증가하였다. 폐경 원인에 따라 골밀도 수치와 골다공증의 유병율을 비교하였을 때 자연 폐경 군(n=950)과 수술적 폐경 군(n=113) 간에 유의한 차이가 있었으나 연령을 계층화하여 보정하였을 때 양군간에 통계적으로 유의한 차이를 발견할 수 없었다. 수술적 폐경 군의 평균 폐경 연령은 47.6±3.6세로 정상 폐경 군의 49.2±3.5세에 비해 유의하게 낮았다. 연령, 폐경기간, 체중, 신장, 출산수가 골밀도와 유의한 상관관계를 보였으나 폐경기간, 체중 및 신장이 다른 인자들의 영향을 고려한 후에도 골다공증에 유의한 영향을 주는 위험인자임을 알 수 있었다. 폐경 후 연간 골소실율은 자연 폐경 군에서 요추와 대퇴경부가 각각 1.2%와 1.1%였고, 수술적 폐경 군의 경우 각각 1.5%와 1.2%였다. 이상에서 본원 폐경기 클리닉을 방문한 폐경 여성의 골밀도 양상을 알아보았다. 수술적 폐경 군과 자연 폐경 군의 경우 연령을 보정하였을 때 골밀도나 골다공증의 유병율에 있어서 유의한 차이가 없었다. 그러나 수술적 폐경은 폐경연령을 앞당김으로써 골다공증의 위험인자가 될 수 있겠다. 이 외에도 폐경기간과 체중 및 신장은 골다공증에 대한 위험인자임을 알 수 있었는데 호르몬 치료를 받기 전 폐경 여성의 14.7%가 이미 골다공증에 이환 되어 있는 것을 고려한다면 일반 폐경 여성은 물론이고 특히 고위험군에서는 유의한 골소실이 진행되기에 앞서 골소실과 골절예방을 위한 보다 적극적인 중재가 필요하리라 생각된다. Objective : To investigate the bone mineral density(BMD) in Korean postmenopausal women. Methods : We performed the retrospective study for 1,508 postmenopausal women who had menopause after 40 years of age and didn't take any hormonal agent before measuring bone mineral density. Spinal and femur neck BMD were measured using dual energy X-ray absorptiometry(DXA, Lunar Co.). Results : The mean age of the study subjects was 53.7±5.7 years and the average BMD of the lumbar spine(L2-L4; L24) and femur neck(FN) were 0.996±0.173 g/㎠ and 0.821±0.133 g/㎠, respectively. Of all subjects, 44.1%(665/1,508) had osteopenia and 14.7%(222/1,508) were already osteoporotic. The prevalence of osteoporosis increased with age; 5.2% in 41-50 years of age, 15.6% in fifties, 32.5% in sixties, and 45.5% in over 70 years of age. There was no significant difference in the age-adjusted BMD between naturally menopausal women(n=828) and surgically menopausal women(n=78). The mean age at menopause of surgically menopausal women was significantly younger than that of naturally menopausal women(47.6±3.6 vs 49.2±3.5). Duration of menopause, body weight, and height were revealed to be the significant risk factors for osteoporosis. The annual bone loss rates in lumbar spine and femur neck were 1.2% and 1.1% respectively in naturally menopausal women, and 1.5% and 1.2% in surgically menopausal women. Conclusion : This study established the BMD curve and regression equation in Korean postmenopausal women according to the causes of menopause. There was no significant difference in the mean BMD and the prevalence of osteoporosis between naturally and surgically menopausal women. Duration of menopause, body weight, and height were found to be the risk factors which influence the development of the osteoporosis. So the postmenopausal women who are at increased risks of developing osteoporosis need more active interventions for preventing the osteoporotic fracture.