대황과 감초 병용의 항산화 및 간보호효과

이은혜,백수연,김광연,이슬기,김상찬,이형식,김영우,Lee, Eun Hye,Baek, Su Youn,Kim, Kwang-Youn,Lee, Seul-Gi,Kim, Sang Chan,Lee, Hyeong Sik,Kim, Young Woo 대한한의학방제학회 2016 大韓韓醫學方劑學會誌 Vol.24 No.3

Objectives : Rheum undulatum Linne and Glycyrriza uralensis Fischer are widely used herbal medicine. In this study, anti-oxidant and liver protective effects of R. undunlatum extract (RUE) and G. uralensis extract (GUE) were investigated in HepG2 cells, respectively. Oxidative stress and liver fibrosis were induced by arachidonic acid (AA) and iron, and CCl₄.Methods : MTT assay was assessed for cell viability, and immunoblotting analysis was performed to detect expression of apoptosis related proteins. In addition, reactive oxygen species (ROS) and mitochondrial dysfunction were measured. In vivo, BALB/c mouse were orally administrated with the aqueous extract of 10 mg/kg RUE and 100 mg/kg GUE for 3 days and then, injected with CCl₄ 0.5 ml/kg body weight to induce acute liver damage. Serum ALT level was measured, and histological change was observed in Harris's hematoxylin and eosin stainResults : RUE and GUE pre-treatment increased relative cell viability in concentration dependent manner and altered the expression levels of apoptosis-related proteins such as procaspase 3, PARP and Bcl-xL. RUE and GUE also inhibited the mitochondrial dysfunction and excessive reactive oxygen species (ROS) production induced by AA and iron. In addition, RUE and GUE activated liver kinase B1 (LKB1), by increasing phosphorylation. Moreover, RUE and GUE treatment decreased liver injuries induced by CCl₄, as evidenced by decreases in histological liver damage as well as serum alanine amino transferase (ALT) level.Conclusions : These data suggest that RUE and GUE has anti-oxidant and liver protective effects against AA and iron-induced oxidative stress and CCl₄-induced liver injury.

주거공간 내 IT기술 적용 에너지 저감 창호형 환기시스템 연구

이은혜,김용성,지충구,Lee, Eun-Hye,Kim, Yong-Seong,Ji, Chung-Gu 한국주거학회 2013 한국주거학회 논문집 Vol.24 No.2

This study has the purpose to adapt IT technology on Window Type Ventilation System for the energy saving and providing of user-centered comfortable environment. This is Derived a look at the case of the window type ventilation system and researched its IT technology for reducing energy applied to the Green Home. This indicates a solution for the established Window Type Ventilation System which can not be satisfied with user's requirement by proposing Window Type Ventilation System applied to IT technology that makes it control the intelligent, combined indoor environment system and providing information. Also, it shows energy saving efficiency of Window Type Ventilation System applied to IT technology based on the model study, analysing the performance of air-conditioning and ventilation energy saving through the experiment to compare with the established Window Type Ventilation System. The result of this study has the significance that it suggests an alternative for energy saving of housing space.

방사성의약품 검사 시 진단(CT)영상에 미치는 영향

이은혜,이예슬,김가중,최준구,Lee, Eun-Hye,Lee, Ye-Seul,Kim, Gha-Jung,Choi, Jun-Gu 대한디지털의료영상학회 2010 대한디지털의료영상학회논문지 Vol.12 No.2

This research attempts to qualitatively evaluate the intensity change by radiopharmaceuticals and obtain computed tomography using phantom injected with various nuclide. Cylindrical phantom is used for comparing and analysing the effect on diagnosis image during radiopharmaceuticals inspection. Inside of the phantom, water is injected and computed tomography image is scanned. During nuclear medicine invitro, frequently used radiopharmaceuticals, $^{99m}TcO_4$ 20 mCi and $^{18}F$ 14 mCi, is diluted in the water phantom and scanned in the same method. Traverse image obtained by CT scan is divided into six traverse image in the same slice of each scanned image. CT-number(HU) value of 10 measuring point is measured in 2 cm interval based on the center of the phantom. Measured HU value, based on the water phantom, is compared with the image after injecting $^{99m}TcO_4$ and $^{18}F$. Average scale of water is 2.8~1.6 HU, $^{99m}TcO_4$ is 3.0~1.6 HU and $^{18}F$ is 1.2~0 HU. Average of water is $2.3{\pm}0.17$ HU, $^{99m}TcO_4$ is $2.2{\pm}0.85$ HU and F-18 is $0.7{\pm}0.95$ HU. Based on water, reduced value of about 0.1 HU and about 0.5 HU is acquired from $^{99m}TcO_4$ and F-18. Radionuclide used in nuclear medicine inspection utilizes 100~200 KeV energy and obtains image through scintillation camera and PET-CT utilizes 511 KeV positron annihilation energy to obtain image. What we learned from this research is that gamma rays from these energies used in CT scan for diagnosis purpose or radioactive therapy plan can change the intensity of the image. The nuclear medicine inspection for reducing the effect of emitted gamma ray diagnosis image should be obtained after a period of time considering half-life which would be reduced distortion or changed in image.

소아 만성 B형 간염 환아에서 라미부딘의 치료 효과

이은혜,장주영,김경모,Lee, Eun-Hye,Jang, Joo-Young,Kim, Kyung-Mo 대한소아소화기영양학회 2008 Pediatric gastroenterology, hepatology & nutrition Vol.11 No.2

목 적: 소아 만성 B형 간염 환아들에서 라미부딘 치료의 효과와 지속성에 대해 알아보고자 하였다. 방 법: 1998년 1월부터 2008년 5월까지 서울아산병원 소아과에서 만성 B형 간염으로 진단받고 라미부딘치료를 받은 44명의 환아들의 의무기록을 후향적으로 분석하였다. 12개월 이상 추적 관찰했던 환아들을 대상으로 발병시 성별, 연령, 치료 전후의 ALT, 혈청 HBVDNA, HBeAg, anti HBe, HBsAg, anti HBs의 변화를 조사하였고, 치료의 지속성과 내성 발현에 대해 조사하였다. 결 과: 라미부딘 치료 후 3년 이내에 총 44명의 환아중 21명(48%)에서 혈청 전환이 이루어졌고, 34명(77%)에서 HBV DNA가 음전되었으며, 41명(93%)에서 혈청ALT가 정상화되었다. Kaplan-Meier법에 의한 HBeAg 누적 혈청 전환율은 3년째에 60%로 나타났다. 치료를 마친 환아 25명 중 혈청 전환된 18명의 환아 모두 약물중단 이후 최대 3년 추적 관찰시 재발 없이 지내고 있어 라미부딘 치료 반응의 지속성을 보여 주었다. 치료 중 12명(27%)에서 돌파 현상이 나타났고, 11명(25%)에서 YMDD 돌연변이가 발견되었다. 치료 전 혈청 ALT 수치는 혈청 전환을 이룬 군에서 더 높았지만, 통계적 유의성은 없었다(338${\pm}$542 IU/L vs. 144338${\pm}$163 IU/L, p> 0.05). 결 론: 만성 B형 간염 환아에서 라미부딘 치료시 절반 정도에서 치료 효과를 기대할 수 있었다. 라미부딘의 치료 반응은 장기적으로 지속되었으며, 향후 약제 내성 돌연변이의 증가에 따른 이차 약제에 대한 추가적인 연구가 필요하다고 생각된다. Purpose: Lamivudine is known to be effective for the treatment of chronic hepatitis B in adults. However, data on lamivudine therapy in pediatrics is limited. The aim of this study was to evaluate the efficacy and durability of lamivudine therapy for chronic hepatitis B in Korean children. Methods: A total of 44 children (27 males and 17 females, ages 6 months to 14.8 years, mean age 6.7 years) with chronic hepatitis B who received lamivudine (3 mg/kg/day, max 100 mg) for at least 12 months were enrolled. We evaluated the serum AST, ALT and serological HBV markers (HBsAg and anti-HBs, HBeAg and anti HBe, and HBV DNA) periodically. Predictive three year cumulative seroconversion rates were obtained using the Kaplan-Meier method. Results: Twenty one (48%) of 44 children achieved seroconversion of HBeAg by three years, while 23 (42%) children did not. HBV DNA was cleared in 34 (77%) children and the serum ALT levels were normalized in 41 children (93%). The three year cumulative seroconversion rates were 60% for HBeAg, and the clearance rates were 76% for HBV DNA. Eighteen children who discontinued lamivudine after HBeAg seroconversion maintained the therapeutic response for three years (treatment duration 13~58 months mean 24 months). Viral breakthrough developed in 12 children (27%) during the therapy and the YMDD mutation was documented in 11 children (25%). The mean duration for the development of a mutation was 22.7 months. Loss of HBsAg occurred in 6 children (14%). The pretreatment ALT levels were higher in responders; however, the differences were not statistically significant (p>0.05). Conclusion: The results of this study showed that lamivudine treatment had a favorable effect and durable therapeutic response in children with chronic hepatitis B. Long term follow-up and alternative therapy are warranted for those patients who do not respond to this treatment.

AlAs_xSb_1-x 단계 성분 변화 완충층을 이용한 Si (100) 기판 상 Al_0.3Ga_0.7As/GaAs 다중 양자 우물 형성

이은혜,송진동,연규혁,배민환,오현지,한일기,최원준,장수경,Lee, Eun Hye,Song, Jin Dong,Yoen, Kyu Hyoek,Bae, Min Hwan,Oh, Hyun Ji,Han, Il Ki,Choi, Won Jun,Chang, Soo Kyung 한국진공학회 2013 Applied Science and Convergence Technology Vol.22 No.6

실리콘(Silicon, Si) 기판과 $Al_{0.3}Ga_{0.7}As$/GaAs 다중 양자 우물(multiple quantum wells, MQWs) 간의 격자 부정합 해소를 위해 $AlAs_xSb_{1-x}$ 층이 단계 성분 변화 완충층(step-graded buffer, SGB)으로 이용되었다. $AlAs_xSb_{1-x}$ 층 상에 형성된 GaAs 층의 RMS 표면 거칠기(root-mean-square surface roughness)는 $10{\times}10{\mu}m$ 원자 힘 현미경(atomic force microscope, AFM) 이미지 상에서 약 1.7 nm로 측정되었다. $AlAs_xSb_{1-x}$/Si 기판 상에 AlAs/GaAs 단주기 초격자(short period superlattice, SPS)를 이용한 $Al_{0.3}Ga_{0.7}As$/GaAs MQWs이 형성되었다. $Al_{0.3}Ga_{0.7}As$/GaAs MQW 구조는 약 10 켈빈(Kalvin, K)에서 813 nm 부근의 매우 약한 포토루미네선스(photoluminescence, PL) 피크를 보였고, $Al_{0.3}Ga_{0.7}As$/GaAs MQW 구조의 RMS 표면 거칠기는 약 42.9 nm로 측정되었다. 전자 투과 현미경(transmission electron microscope, TEM) 단면 이미지 상에서 AlAs/GaAs SPS 로부터 $Al_{0.3}Ga_{0.7}As$/GaAs MQWs까지 격자 결함들(defects)이 관찰되었고, 이는 격자 결함들이 $Al_{0.3}Ga_{0.7}As$/GaAs MQW 구조의 표면 거칠기와 광 특성에 영향을 주었음을 보여준다. The $AlAs_xSb_{1-x}$ step-graded buffer (SGB) layer was grown on the Silicon (Si) substrate to overcome lattice mismatch between Si substrate and $Al_{0.3}Ga_{0.7}As$/GaAs multiple quantum wells (MQWs). The value of root-mean-square (RMS) surface roughness for 5 nm-thick GaAs grown on $AlAs_xSb_{1-x}$ step-graded buffer layer was ~1.7 nm. $Al_{0.3}Ga_{0.7}As$/GaAs MQWs with AlAs/GaAs short period superlattice (SPS) were formed on the $AlAs_xSb_{1-x}$/Si substrate. Photoluminescence (PL) peak at 10 K for the $Al_{0.3}Ga_{0.7}As$/GaAs MQW structure showed relatively low intensity at ~813 nm. The RMS surface roughness of the $Al_{0.3}Ga_{0.7}As$/GaAs MQW structure was ~42.9 nm. The crystal defects were observed on the cross-sectional transmission electron microscope (TEM) images of the $Al_{0.3}Ga_{0.7}As$/GaAs MQW structure. The decrease of PL intensity and increase of RMS surface roughness would be due to the formation of the crystal defects.

수종의 섬유 강화 레진 포스트의 방사선 불투과도와 식별도 평가

이은혜,최항문,박세희,김진우,조경모,Lee, Eun-Hye,Choi, Hang-Moon,Park, Se-Hee,Kim, Jin-Woo,Cho, Kyung-Mo 대한치과보존학회 2010 Restorative Dentistry & Endodontics Vol.35 No.3

본 연구의 목적은 수종의 섬유 강화 레진 포스트의 방사선 불투과도와 식별도를 비교하고자 하는 것이다. 이번 실험을 위해 1) FRC Postec Plus (Ivoclar Vivadent AG, Schaan, Liechtenstein) 2) Snowlight (Carbotech, Lewis center, OH, USA) 3) Dentin Post (Komet Brasseler, Lamgo, Germany) 4) Rely-X Fiber Post (3M ESPE, St.paul, MN, USA) 5) D.T.-Light Post (BISCO, Schaumburg, IL,USA ) 6) Luxapost (DMG, Hamburg, Germany) 등 6종의 섬유 강화 레진 포스트를 사용하였다. 각 포스트를 참고 기준으로 사용한 1 mm, 2 mm 간격의 알루미늄 step-wedge와 함께 디지털센서를 사용하여 방사선 촬영하였다. 방사선 불투과도는 각 포스트의 다섯 지점에서 $10{\times}10$ 화소의 평균 회색조 값으로 계산하였고 동일 두께의 알루미늄 당량으로 비교하였다. 치아 시편으로 비슷한 방사선 불투과도를 보이는 상악 전치 6개에 포스트를 식립 후 건조 하악골의 설측에 위치시켜 방사선 사진을 촬영하였다. 포스트만을 촬영한 사진과 치아 시편의 방사선 사진들을 구강악안면방사선과 전공자 3명, 치과보존과 전공자 3명, 일반 치과 의사 3명에게 보여주고 설문지를 작성하도록 요청하였다. 설문지는 포스트와 포스트가 식립된 치아 시편 사진에서 방사선 불투과도가 가장 높은 포스트와 낮은 포스트를 고르는 문항, 치근단과 치관부 끝에서 인접 상아질과 가장 식별이 잘되는 포스트를 고르는 문항을 포함하였다. 실험의 결과는 다음과 같았다. 1. 각 FRC-Post는 다양한 방사선 불투과도를 보였다. 2. 가장 높은 방사선 불투과도와 가장 낮은 방사선 불투과도를 가지는 포스트를 고르는 질문에서 포스트만 나열한 경우 실험군 모두 100%의 정답률을 보였으나 포스트를 치아에 넣고 치조골을 중첩시킨 경우 정답률의 변화를 보였다. 3. 포스트 주변 상아질과 구분되는 정도를 묻는 질문에 대해 시편의 조합 순서를 바꾸자 일관된 식별 결과를 보이지 않았다. 이에 본 연구에 사용한 포스트는 다양한 방사선 불투과도를 보이나 식별 능력에 영향을 미칠 정도의 방사선 불투과도 차이를 보이지 않는 것으로 사료된다. The purpose of this study was to compare radiopacity and radiographic discriminability of various FRC-Posts. Six FRC-Posts were investigated ; 1) FRC Postec Plus (Ivoclar Vivadent AG, Schaan, Liechtenstein), 2) Snowlight (Carbotech, Lewis center, OH, USA), 3) Dentin Post (Komet Brasseler, Lamgo, Germany), 4) Rely-X Fiber Post (3M ESPE, St.paul, MN, USA), 5) D.T.-Light Post (BISCO, Schaumburg, IL,USA), 6) Luxapost (DMG, Hamburg, Germany) The radiographs of each post with a reference 1 mm / 2 mm aluminum step-wedge was taken using digital sensor. The optical density were calculated by gray value of $10{\times}10$ pixel and compared in mm Al equivalent at five points. Six maxillary incisors of similar radiopacity were used. Radiographs of posts in Mx. incisors of lingual side of dry mandible were taken. We showed radiographs and asked the questionnaire to 3 radiologists, 3 endodontists, 3 general practitioners. The questionnaire was comprised of choices of the highest, lowest radiopaque individual post and the choices of best discriminable post at apical, coronal area. The following results were obtained. 1. Each post system showed various radiopacity. 2. There was change of discriminability between each post and simulated specimens regardless of examiner. Although each post showed various radiopacity, the difference of radiopacity did not affect on discriminability.

Alg₃ 두께 변화에 따른 유기 발광 소자의 내장 전압

이은혜,윤희명,김태완,Lee, Eun-Hye,Yoon, Hee-Myoung,Kim, Tae-Wan 한국전기전자재료학회 2008 전기전자재료학회논문지 Vol.21 No.3

Built-in voltage in ITO/$Alq_3$/ Al organic light-emitting diodes was studied by varying a thickness of $Alq_3$ layer using modulated photocurrent technique at ambient condition. A thickness of the $Alq_3$ layer was varied from 100 to 250 nm. From the bias voltage-dependent photocurrent, built-in voltage of the device was able to be determined. The obtained built-in voltage is about 0.8 V irrespective of the $Alq_3$ layer thickness in the device. This value of built-in voltage confirms that the built-in voltage is generated due to a difference of work function of the anode and cathode. The $Alq_3$ layer thickness independent built-in voltage indicates that the built-in electric field in the device is uniform across the organic layer.

고추장 묘사분석을 위한 프로토콜 개발: 입가심물질 중심으로

이은혜,정서진,유선미,한귀정,Lee, Eun-Hye,Chung, Seo-Jin,Yu, Seon-Mi,Han, Kui-Jeong 한국식품조리과학회 2013 한국식품조리과학회지 Vol.29 No.5

This study was conducted to establish an effective palate cleanser when conducting descriptive analysis for gochujang products. In addition, descriptive analysis procedure for gochujang products was optimized. A generic descriptive analysis was performed on 4 types of gochujang samples varying in hot and spicy levels. The sensory attributes developed were 9 odors, 13 flavors, 4 texture and mouth feel attributes, and 4 appearance attributes. In order to select an effective palate cleanser for gochujang, 5 types of cleansers were tested (water, water+bread, water+cucumber, water+milk, water+cracker). Correct answering rate, significant numbers of product effect on sensory attributes, and the mean hot and spicy intensity values were considered to select the optimal palate cleanser. Results showed that as the hot & spicy level increased, red pepper odor and flavor significantly increased whereas umami taste intensity decreased. When comparing the efficiencies of various palate cleanser, the correct answer rates were the highest when warm water was used with either cucumber or cracker. Additionally, the attribute intensities were better differentiated between gochujang samples when cracker, white wheat bread, or cucumber were used. Overall, warm water with cucumber or cracker were shown to be the most effective cleansers.

한국인 메틸말로닌산뇨증 및 프로피온산뇨증의 유전자형과 임상 양상

이은혜,고정민,김재민,유한욱,Lee, Eun Hye,Ko, Jung Min,Kim, Jae-Min,Yoo, Han-Wook 대한소아청소년과학회 2008 Clinical and Experimental Pediatrics (CEP) Vol.51 No.9

목 적 : 메틸말로닌산뇨증과 프로피오닌산뇨증은 상염색체 열성으로 유전되는 아미노산 대사 이상 질환으로, methylmalonyl-CoA mutase와 propionyl-CoA carboxylse의 결함에 의해 발생하며 최근 유전자형에 대한 연구가 활발히 이루어지고 있다. 저자들은 단일기관에서 경험한 이 질환군의 임상 양상과 유전자형에 대해 조사하고자 하였다. 방 법 : 1993년부터 2007년까지 서울아산병원 소아과에서 유기산뇨증으로 진단된 20례를 대상으로 질병의 종류, 진단시 연령과 임상 양상, 유전자형, 검사 소견, 치료와 예후 등을 후향적으로 분석하였다. 혈장 암모니아, 소변 유기산 분석과 혈장 아미노산을 조사하였고, 유전자분석은 메틸말로닌산뇨증에서는 MUT, MMAA, MMAB 와 MMACHC 유전자를, 프로피오닌산뇨증에서는 PCCA와 PCCB 유전자를 분석하였다. 결 과 : 유기산뇨증으로 진단된 환아는 모두 20명이었으며, 그 중 메틸말로닌산뇨증이 12명(남아 8명, 여아 4명), 프로피오닌산뇨증이 8명(남아 6명, 여아 2명)이었다. 신생아 대사이상 검사로 진단된 환아가 5명이었으며, 6명은 신생아기에 급성 증상으로 발현하였고, 9명은 1개월 이후 발현한 지발형이었다. 신생아기 발현형에서는 6명 중 5명이 구토와 기면을 주증상으로 내원하였으며, 지발형에서는 구토와 기면 이외에도 발달 지연, 보행 장애, 혈소판 감소증 등 다양한 임상 양상을 보였다. 예후로는 메틸말로닌산뇨증 환아 중 2명(17%)이 2세경에 고암모니아혈증과 대사성 산증으로 사망하였으며, 7명(58%)이 정상발달을 보였다. 프로피오닌산뇨증 환아는 1명이 사망하였고, 4명(50%)이 정상 발달을 보이고 있다. 증상의 발현시기에 따라서는 신생아기 발현형에서 6명중에서 2명은 사망, 2명은 정상 발달, 2명은 발달지연을 보이고 있는 것에 비해, 증상 없이 신생아 대사이상 검사로 진단된 환아 중에는 2명(40%)이 정상발달을 보이고 있고, 지발형에서는 7명(63%)이 정상 발달을 보이고 있다. 유전자형은 메틸말로닌산뇨증 전례에서 규명되었으며 10명은 MUT 유전자에서 11종의 서로 다른 돌연변이가 발견되었는데 대부분 nonsense 돌연변이였다. 비타민 B12 반응형 환아 2명에서는 MMACHC 유전자에서 3종의 서로 다른 돌연변이가 발견되었으며 프로피오닌산뇨증에서는 16개 대립유전자 중 14개에서 PCCA와 PCCB 유전자의 돌연변이가 규명되었다. 결 론 : 유기산뇨증은 진단이 지연되면 매우 치명적이나, 조기에 의심하여 진단하고 적절히 치료하면 좋은 예후를 기대할 수 있는 질환이다. 유기산뇨증의 유전자형의 분석은 정확한 진단을 가능하게 할 뿐 아니라 유전상담, 산전 진단 및 표현형의 예측에도 도움이 된다. Purpose : Methylmalonic aciduria (MMA) and propionic aciduria (PA) are inborn errors in the catabolism of branched-chain amino acids. The study was undertaken to investigate the genotypes and clinical features of Korean patients with MMA and PA. Methods : This study examined 12 patients with MMA and eight with PA. We analyzed various clinical features, laboratory findings, treatments, and neuro-developmental outcomes. Diagnoses were based on the presence of characteristic compounds detected by amino acid analysis in serum and organic acid analysis in urine. Mutation analysis was performed in the genes of MUT, MMAA, MMAB, and MMACHC for MMA and PCCA and PCCB for PA. Results : Among the 20 patients, six patients were diagnosed before one month of age and nine patients were diagnosed after the newborn period. Five patients were diagnosed via a neonatal screening test. Patients with early-onset forms had more severe illness at presentation and generally poor outcomes. A favorable outcome was obtained in 55% patients; most of them were of a late-onset type or diagnosed by neonatal mass screening test without symptoms. Genotypes were confirmed in all patients with MMA. We detected 11 different mutations by MUT gene analysis in 10 patients, and three different mutations in MMACHC genes in two patients. PCCA and PCCB gene mutations were identified in 14 of the 16 alleles, in eight patients with PA. Conclusion : Organic aciduria is a fatal disease; however, better outcomes are expected whenever early diagnosis and prompt management are made possible. Mutation analysis is useful for confirming diagnoses and planning management strategies.

소아에서 내시경 검사의 출혈 위험 예측 인자로서 혈액 응고 검사 및 혈소판 검사의 의의

이은혜,양혜란,고재성,서정기,Lee, Eun-Hye,Yang, Hye-Ran,Ko, Jae-Sung,Seo, Jeong-Kee 대한소아소화기영양학회 2010 Pediatric gastroenterology, hepatology & nutrition Vol.13 No.1

목 적: 본 연구에서는 소아의 내시경 시술 전 출혈의 위험성을 예측하는 검사로 혈소판과 혈액 응고 검사의 임상적 의의를 평가하고자 하였다. 방 법: 2004년 3월부터 2009년 12월까지 분당서울대학교병원 소아청소년과 외래 및 입원 환자 중에서 위장관 내시경 검사를 시행 받은 소아청소년 환자의 의무기록과 혈소판 수치 및 혈액 응고 검사 결과들을 후향적으로 분석하였다. 결 과: 총 1,476건의 검사 결과, 혈소판 수치가 정상보다 낮은 경우는 1명이었고, PT INR만이 비정상으로 연장된 경우가 25건(1.7%)이었고, aPTT만 연장된 경우가 132건(9%)이었고, 둘 다 연장된 경우가 5건(0.3%)이었다. aPTT 결과가 비정상이었던 경우에 혈액 응고 인자 검사를 시행한 경우가 14건이었고, 이 중 혈액 응고인자 XII 결핍증으로 진단된 경우가 7명, 폰 빌레블란드 병으로 진단된 경우가 1명, 폰 빌레블란드 병과 혈액 응고 인자 XII 결핍증이 동시에 나타난 경우가 1명, 경증의 A형 혈우병이 의심된 경우가 1명이었으며, 나머지 4명은 정상이었다. 혈액 응고 질환으로 진단된 환자를 포함하여 내시경 시술을 받은 모든 소아 환자에서 의미 있는 출혈 합병증이 나타난 경우는 없었다. 결 론: 소아 내시경 사전 검사로 시행하는 혈액 응고검사에서 일부 이상 소견이 확인되더라도 실제 혈액 응고 질환으로 진단되는 경우는 드물고, 혈액 응고 질환으로 진단된 환자에서조차 내시경 시술에 의한 출혈 합병증이 나타나지 않았다. 따라서 내시경 시술 전에 모든 환자를 대상으로 혈액 응고 검사 및 혈소판 검사를 반드시 시행할 필요는 없을 것으로 여겨지며, 출혈 경향을 나타내는 과거력과 가족력이 있는 소아와 이전에 혈액 응고 검사를 한 번도 시행 받지 않았던 영유아 환자에서 내시경 시술 전에 혈액 응고 검사를 시행하는 것이 바람직할 것이다. Purpose: The aim of this study was to assess the benefits of routine pre-endoscopy coagulation screening tests and platelet counts in Korean children. Methods: Between March 2004 and December 2009, children who underwent gastrointestinal endoscopy for the evaluation of various gastrointestinal symptoms were included. All of the subjects included in the study also underwent routine coagulation screening and platelet count determinations prior to endoscopy and biopsy. The clinical records and laboratory tests were retrospectively reviewed in all patients. Results: One hundred sixty-two of 1,476 (11%) patients who underwent endoscopy had abnormal results on pre-screening coagulation tests. Fourteen patients underwent coagulation factor assays due to abnormal clotting results in consecutive tests or due to clinical evidence of a bleeding tendency. Seven patients were diagnosed with factor XII deficiency, one patient was diagnosed with von Willebrand disease, one patient had von Willebrand disease and factor XII deficiency, and one patient was presumed to have mild hemophilia. The remaining 4 patients had normal results with the factor assays. The results of platelet counts were normal with the exception of 1 patient. No patient had significant bleeding during the endoscopic procedures, despite abnormal pre-endoscopic coagulation tests. Conclusion: Routine coagulation screening tests and platelet counts revealed abnormal results in some patients. Most of the patients with abnormal clotting were shown to have a factor XII deficiency, which had no significant associated bleeding tendencies; the other patients were diagnosed with hemophilia or von Willebrand disease. Therefore, although abnormal pre-endoscopic coagulation is not always related to significant bleeding complications, pre-endoscopic coagulation screening may be useful in some children in predicting the risk of bleeding tendency during endoscopic procedures.