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버어리종담배 건조엽의 부패세균 Erwinia carotovora subsp. carotovora의 동정 및 부패환경에 관한 연구
강여규,김정화,김요태,Kang, Yeo-Gyu,Kim, Jeong-Hwa,Kim, Yo-Tae 한국연초학회 1985 한국연초학회지 Vol.7 No.2
The incitant of soft rot on burley tobacco leaves in the curing vinylhouse was identified as Erwinia carotovra subsp. carotovora on the basis of its physiological characters. The bacterium yew best at $25^{\circ}C$ and $30^{\circ}C$, but no growth was detectable at $40^{\circ}C$ in the nutrient broth for 24 hours period. Burley tobacco leaves inoculated with the bacteria (Ecc) produced typical soft rot lesions when the incubation temperature was 25 to $30^{\circ}C$ and the relative humidity was more than 8075, however, the lesion development was suppressed when the temperature was $40^{\circ}C$ and the relative humidity was below 80%. Significant negative correlation was found between hanging space in the curing vinylhouse and the incidence of soft rot on the tobacco leaves harvested in a rainy day regardless of streptomycin treatment.
담배줄기 속썩음병균 Erwinia carotovora subsp. carotovora의 토양중에서의 월동
강여규,박은경,추홍구,Gang, Yeo-Gyu,Park, E.K.,Chu, H.G. 한국연초학회 1989 한국연초학회지 Vol.11 No.1
The significance of soil and/or rhisosphere populations of Erwinia carotovora sobsp. carotovora (Ecc) as a source of primary inoculum for tobacco hollow stalk disease has been demonstrated conclusively. The survival of Ecc in field soils fter overwintering was estimated by using the enrichment technique. The population number of pectolytic erwinia (PE) in field soils relatively decreased at the rate of 102-104 colony forming unit(CFU) per g of soil after overwintering. Higher level of PE population overwintered in the rhlzosphere foils of tobacco stubbles and detected more frequently in rhizosphere soils of weed plants than in those of bare fields. All of the tobacco stubbles collected from fields where tobacco had been grown the previous year contained Ecc. The more survived population number of PE at the 30cm depth of artifitiany infested soils than at the upper of those by introducing with diseased tobacco plant tissue after overwintering. Ecc overwintered effectively in rhizosphere soils of tobacco stubbles, overwintered weeds and tobacco debris in field soils.
Metalaxy1 저헝성 담배역병균(Phytophthora nicotianae) 방제를 위한 약제 선발
강여규,정윤화 한국식물병리학회 2003 식물병연구 Vol.9 No.4
Five commercial chemicals, Dimethomorph, Foseyl-Al, Oxadixyl +Mancozeb, Propamocarb hydrochloride, and Metalaxyl, were tested for control of tobacco black shank (Phytophthora nicotianae) and/or delaying buildup of the resistant population to Metalaxyl which has been used for control of the disease in Korean tobacco farms. Propamocarb hydrochloride seemed to have a cross-resistance to the Metalaxyl resistant isolates showing similar response with Metalaxyl in vitro. Meanwhile, Dimethomorph+Mancozeb and Oxadixyl+ Mancozeb were selected to be the promising chemicals which are able to be alternative to Metalaxyl for the black shank control in accordance with in vitro and in field trial.
버어리종담배 산지의 Streptomycin 耐性 담배줄기속썩음병균 Erwinia carotovora subsp. carotovora의 발생
강여규 한국연초학회 1996 한국연초학회지 Vol.18 No.1
Erwinia carotovora subsp. Carotovora (Ecc), a pathogen of tobacco hollow stalk disease, was isolated for testing susceptibility to streptomycin from diseased plants in burley tobacco growing area. Of 157 isolates tested, 17 isolates (108%) were resistant to the antibiotic at the antibiotic from field soils, which streptomycin had been used continuously for three years for control of the disease was three times higher than those of non-used. There was no difference in virulence and generation time between streptomycin-sensitive and resistant strains.
버어리종 담배 포장에서의 가칭 담배 묘썩음병(Pythim spp.) 발생 및 방제에 관한 연구
강여규 한국연초학회 1991 한국연초학회지 Vol.13 No.1
Seedling rot occurred on burley tobacco at the early growing stage after transplanting was surveyed in the Chonbuk province, the main area for burley tobacco production, during the 3 crop years from 1986. The incidence of disease was about 0.6-0.7% every year and major causal organisms were Pythium spp. More diseased plants were observed in the fields in which tobacco planted with improved mulching system than in that with any other mulching system. The temperature and relative humidity in the planting hole covered with polyethylene film for 11 days after transplanting were recorded by 27-45$^{\circ}C$ and 75~95%, respectively. The punched polyethylene film mulching field showed less diseased plants than that with improved mucking. In improved mulching system the longer period of mulching caused higher incidence of seedling rot(r=0.74**). Soil drench treatment of 200m1 of aqueous Metalaxyl Solution(125ppm) was effective to control the disease.
Streptomycin 내성 담배줄기속썩음병균 방제약제 선발
강여규 한국연초학회 1996 한국연초학회지 Vol.18 No.1
Chemicals including antibiotics and bactericides were screened for suppression of streptomycin-resistant Erwinia cmutouom subsp. cmutovom (Ecc) strains in laboratory and field conditions. Oxytetracycline, ethoquinolac and dichlorophen suppressed the growth of streptomycin-resistant Ecc strains in vitro. Fractional inhibitory concentration (FIC) indices of oxytetracycline and ethoquinolac mixed with streptomycin against the Ecc strains were equal to and less than one, respectively. Consequently the efficacy of those chemicals in mixture with sorptomycin were non-antagonistic But that of dichlorophen mixed with streptomycin was more than one, therefore the efficacy of the mixture was antagonistic. Spray of oxyteoucycline, ethoquinolac and agrimycin-100 on the topped burley tobacco plants was efficacious in reducing tobacco hollow stalk at the same level of sorptomycin treatment in three-year field trials, which suggests that those are promising chemicals to be alternative to streptomycin for control of tobacco hollow stalk.
강여규,유연현,정연화 한국식물병리학회 2004 Plant Pathology Journal Vol.20 No.4
The population of Ralstonia solanacearum (Rs) in soil is very important as a primary inoculum source of bacterial wilt in tobacco fields. To investigate the population of Rs, physical properties and chemical components during the tobacco growing season, soil samples were taken from the fifteen fields which were located in the flue-cured tobacco growing area, Ansung, Kyunggi province and Wonju, Kangwon province. Two fields of the fifteen were bacterial wilt free. Six fields had less than 10% plants being diseased and seven over 10%. The Rs population level determined by using SMSA medium generally showed an up-and-down pattern being low in May, high in Jun and July and low in August. The soil population in May and June showed a positive correlation with the incidence of bacterial wilt (r=0.571*, r=0.688**), but P2O5 content of soil was negatively correlated with the disease incidence (r= -0.539*). These results suggest that Rs population in soil examined in May or in June, and the P2O5 content in soil should be key factors to determine the bacterial wilt potential of tobacco fields.
수원과 전주 지방에서 blacklight trap에 유인된 우단풍뎅이아과, 검정풍뎅이아과 및 줄풍뎅이아과 풍뎅이의 종류와 발생 소장
김기황,강여규 한국응용곤충학회 1993 한국응용곤충학회지 Vol.32 No.4
Species and seasonal occurrences of Sencinae, Melolonthinae and Rutelinae chafers captured b by blacklight traps were investigated from 1989 to 1991 in Suwon and Chonju. Numbers of s species captured in Suwon and Chonju were 6 and 6 for Sericinae, 8 and 7 for Melolothinae, 7 a and 8 for Rutelinae, 21 and 21 in total, respectively. Species with more captures from both districts in each subfamily were Maladera orientalis of Sericinae, Holotrichia morosa and H. diomphalia of Melolonthinae, and Anomala corpulenta, and A. rufocuprea of Rutelinae. Adults of t the above five species were captured 1O~20 days earlier in Chonju than in Suwon. H. d diomphalia adults occurred mamly In odd years in Chonju, which is the same pattern as in S Suwon. 1989~1991년에 수원과 전주지방에 blacklight trap에 유인된 우단풍뎅이아과, 검정풍뎅이아과 및 줄풍뎅이아과 풍뎅이의 종류와 발생 소장을 조사하였다. 수원과 전주 지방에서 유인된 종수는 각각 우단풍뎅이아과 6종과 6종, 검정풍뎅이아과 8종과 7종, 줄풍뎅이아과 7종과 8종, 합계 21종과 21종이었다. 두 지방에서 각 아과 중 보다 많이 유인된 종은 우단풍뎅이아과의 애우단풍뎅이, 검정풍뎅이아과의 큰검정풍뎅이와 참검정풍뎅이, 줄풍뎅이아과의 다색줄풍뎅이와 애풍뎅이였다. 위 5종의 성충은 수원 지방보다 전주 지방에서 10~20일정도 빨리 유인되었다. 참검정풍뎅이 성충은 전주 지방에서 주로 홀수해에 발생하여 수원 지방에서와 같은 양상을 보였다.