Rhizopus nigricans 를 이용한 고농도의 Progesterone으로부터 11α- hydroxyprogesterone의 생산

최용복,최상기,김학성,박영훈 한국산업미생물학회 1990 한국미생물·생명공학회지 Vol.18 No.1

부신피질호르몬 제조의 중요한 전구체인 11α-hydroxyprogesterone을 Rhizopus nigricans를 이용하여 고농도의 progesterone으로부터 고수율로 생산하기 위한 연구를 수행하였다. 생물전환수율을 최대로 하는 배지조성은 포도당과 casamino acid의 비율이 2:1인 것으로 관찰되었다. Progesterone은 Tween 80 용액에 분산시켜서 종균배양후 12시간 정도 지나서 첨가하였을 때 전환수율이 높았다. Progesterone의 농도가 증가함에 따라 생산성이 증가하였고 progesterone이 20g/l일 경우 전환수율은 약 70% 정도였다. A study on 11 α-hydroxylation of progesterone by using Rhizopus nigricans was carried out to produce efficiently 11 α-hydroxyprogesterone which is an essential intermediate of corticosteroids synthesis. Firstly, medium was optimized in view of bioconversion yield and cell growth. Glucose and casamino acid were selected as carbon and nitrogen source and the ratio of carbon to nitrogen which maximize bioconversion yield was determined to be 2:1. Secondly, the addition time of progesterone and dispersion method were studied. When progesterone dispersed with 0.01%(v/v) Tween 80 was added at 12-14 hr of cultivation, higher bioconversion yield was obtained. When 20g/l of progesterone was added, the yield reached upto 70% under optimized conditions.

濟州國際自由都市의 生態的 特性을 고려한 生態觀光 活性化 方案 硏究

최용복 국토연구원 2003 국토연구 Vol.36 No.-

Korean government has initiated the special law for Jeju International Free City in order to build Jeju-Do as an international city focusing on tourism and leisure. Even though Jeju has been recognized as the best favorite tourism site possessing the preserved natural resources in the nation, it faces a risk due to the lack of tourism programs and efforts to develop tourism resources. Indeed, since the trends of tourism development have been oriented in a large scale development that caused to a serious destruction of natural environment, social conflicts between developmentalists and environmentalists have been aroused. In these circumstances, ecotourism has been emerged as an alternative tourism that minimizes its impacts upon environment and emphases on ecological sustainability. In this sense, the purpose of this paper is to propose the development alternatives for tourism development under the assumption that ecotourism is the best suitable tourism type for Jeju island. In order to fulfill this purpose, following three subjects have been investigated and analyzed : 1) status of ecotourism with a wide examination of ecological characteristics of Jeju, 2) analysis on recognition of tourists and citizens regarding ecotourism development, and 3) the possible development alternatives extracted from a comprehensive understanding and examination of 1) and 2).

Increased lysine N-methylation of a 23-kDa protein during hepatic regeneration

최용복,고명현,신창호,김경숙,홍경만,백문기,박동은 생화학분자생물학회 2005 Experimental and molecular medicine Vol.37 No.3

The methylation of a 23-kDa nuclear protein increased after partial hepatectomy and methylation returned to basal levels after the initial stage of regeneration. The methylating enzyme was partially purified from rat liver by ammonium sulfate precipitation, DEAE-anion exchange chromatography and Butyl-Sepharose chromatography. The 23-kDa protein was purified from a nuclear fraction of liver tissue with SP-Sepharose. When the 23-kDa protein was methylated with the partially purified methyltransferase and analyzed on C18 high performance liquid chromatography (HPLC), the methylated acceptor amino acid was monomethyl lysine (MML). Previously, only arginine N-methylation of specific substrate proteins has been reported during liver regeneration. However, in this report, we found that lysine N-methylation increased during early hepatic regeneration, suggesting that lysine N-methylation of the 23-kDa nuclear protein may play a functional role in hepatic regeneration. The methyltransferase did not methylate other proteins such as histones, hnRNPA1, or cytochrome C, suggesting the enzyme is a 23-kDa nuclear proteinspecific lysine N-methyltransferase.

Progesterone의 연속첨가에 의한 11$\alpha$-hydroxyprogesterone으로의 생물전환수율의 증대

최용복,김학성,박영훈 한국미생물 · 생명공학회 1990 한국미생물·생명공학회지 Vol.18 No.3

Biotransformation of progesterone to 11 $\alpha$ -hydroxyprogesterone by growing cells of Rhizopus nigricans was investigated. As the concentration of progesterone increased, the specific growth rate of R. nigricans decreased linearly, and consequently the conversion yield lowered. The hyphae of the microorganism were observed to become thicker, shorter, and more densely branched at high concentrations of progesterone. In order to improve the process productivity, biotransformation was conducted with continuous addition of progesterone. When the substrate was added continuously at a rate of 0.86 g/hr for 30 hrs, overall conversion yield reached upto 56% while a single addition of the same amount of progesterone yielded about 40% eonversion. When additional feeding of glucose was carried out upon its depletion, an improved br'oconversion yield upto 68% was obtained.

Rhizopus nigricans를 이용한 고농도의 Progesterone으로부터 11$\alpha$-hydroxyprogesterone의 생산

최용복,최상기,박영훈 한국미생물 · 생명공학회 1990 한국미생물·생명공학회지 Vol.18 No.1

부피질호르몬의 제조의 중요한 전구체인 11alpha-hydroxyprogesterone을 Rhizopus nigricans를 이용하여 고농도의 progesterone으로부터 고수율로 생산하기 위한 연구를 수행하였다. 생물전환수율을 최대로 하는 배지조성은 포도당과 casamino acid의 비율이 2:1인 것으로 관찰되었다. Progesterone은 Tween 80 용액에 분산시켜서 종균배양 후 12 시간 정도 지나서 첨가하였을 때 전환수율이 높았다. Progesterone의 농도가 증가함에 따라 생산성이 증가하였고 Progesterone이 20g/$\ell$일 경우 전환수율은 약 70정도 였다. A study on 11 $\alpha$-hydroxylation of progesterone by using Rhizopus nigricans was carried out to produce efficiently 11 $\alpha$-hydroxyprogesterone which is an essential intermediate of corticosteroids synthesis. Firstly, medium was optimized in view of bioconversion yield and cell growth. Glucose and casamino acid were selected as carbon and nitrogen source and the ratio of carbon to nitrogen which maximize bioconversion yield was determined to be 2:1. Secondly, the addition time of progesterone and dispersion method were studied. When progesterone dispersed with 0.01% (v/v) Tween 80 was added at 12-14 hr of cultivation, higher bioconversion yield was obtained. When 20g/$\ell$ of progesterone was added, the yield reached 70% under optimized conditions.

특집 / 제 17 회 지방행정연수대회 : 우수논문요약소개 / 주제 : 반상회운영강화방안 - 반상회를 통한 새마을금고 육성방안 -

최용복 대한지방행정공제회 1976 地方行政 Vol.25 No.278

국가 도서의 효율적 관리 및 활용을 위한 도서정보 실험시스템 개발

최용복,오승,Choi, Yong-Bok,Oh, Seung 한국공간정보학회 2012 한국공간정보학회지 Vol.20 No.3

전 세계적으로 활용 가치가 나날이 증대되고 있는 도서(섬)에 대한 관심이 날로 높아지고 있으며 영토에 대한 배타성을 갖기 위한 노력이 각 국가별로 활발하게 진행되고 있다. 더욱이 삼면이 바다로 둘러싸인 우리나라의 경우 영토주권 확립 및 국토 환경에 대한 보존, 관리, 관광자원 개발을 위한 도서 관리 체계 구축을 위해서는 도서지역에 대한 명확한 공간정보의 취득과 데이터베이스 구축이 필요한 시점이다. 본 연구에서는 전국 단위의 도서 정보체계를 구축하기 위한 전단계로서, 제주도 인근의 63개 유 무인도를 중심으로 인문지리정보에 관한 각종 현황을 조사하고, 이를 체계적인 관리가 가능한 GIS 기반의 관리 시스템을 개발하였으며, 나아가 국토 도서자원의 다양한 관광 서비스 정보로 제공할 수 있도록 하기 위한 서비스 제공 시스템을 개발하였다. 또한 향후 전국적인 국가 도서정보 시스템으로 발전해 나가기 위해 필요한 방안을 제시하였다. Many countries have interested in islands with their values in nature such as future sustainable resource and new expansion of marine territory. As territorial and cultural assets, these islands have been the source of our national competitiveness and, then, Korean government has initiated island management policy that provides a basis for the development and protection of islands. Consequently, spatial data and database for resource management have been required. In this sense, the purpose of this paper is to investigate the condition and status of various natural resources in Jeju islands. Jeju has 63 islands including 55 uninhabited islands. Then, an Island Information System(IIS) based on geographical information system(GIS) has been proposed. This ISS will provide not only a measure for effective resource management but also systematic services for releasing useful resource information.

지방행정소개 : 전주 『 난장 』 - 반세기만에 재현된 우리민속축제 -

최용복 대한지방행정공제회 1984 地方行政 Vol.33 No.368

`87 내무행정 역점시책 지방재정역점시책

최용복 대한지방행정공제회 1987 地方行政 Vol.36 No.401

N/A

88 내무행정 역점시책 지방행정 역점시책

최용복 대한지방행정공제회 1988 地方行政 Vol.37 No.413

N/A