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Ling‑Hong Miao,Wen‑Jing Pan,Yan Lin,Bo Liu,Ming‑Chun Ren,Qun‑Lan Zhou,Xian‑Ping Ge 한국유전학회 2017 Genes & Genomics Vol.39 No.12
The objective of this study was to analyze the target genes and regulatory function of miR-34a in Megalobrama amblycephala using second-generation highthroughput sequencing and bioinformatic tools. Functional enrichment analysis was performed by gene ontology. MiR- 34a and target gene expression levels were measured in M. amblycephala fed normal and high-carbohydrate diets. The results revealed that miR-34a was highly conserved in several species, and miR-34a of M. amblycephala has a close evolutionary relationship to that of zebrafish and common carp. miRanda, TargetScan, RNAhybrid predicted 5,185, 6,282 and 2,168 target genes, respectively, and 645 target genes were in common. According to annotation information, the target genes were enriched in phosphate metabolism, glycerophospholipid metabolism, Golgi vesicle transport, cell division, and other biological processes (P < 0.05). Pathway enrichment analysis revealed that these target genes were mainly enriched in alpha-linolenic acid and linoleic acid metabolism, ether lipid metabolism, VEGF signaling pathway, Fc epsilon RI signaling pathway, GnRH signaling pathway, and MAPK signaling pathway (P < 0.05). The regulatory role of miR-34a was more significant in the liver than in the brain of M. amblycephala. MiR-34a regulates glucose lipid homeostasis induced by high glucose diets by upregulating hepatic PI3K/Akt, FOXO, and TOR signaling pathways.
Wen-jie Shi,Jian-ling Wang,Dong Yang 연세대학교의과대학 2016 Yonsei medical journal Vol.57 No.2
Purpose: Tumor-associated microRNAs have been detected in cancer, though whether plasma microRNA-155 (miR-155) could be a potential biomarker for laryngeal squamous cell carcinoma (LSCC) prognosis is unclear. We aimed to determine how miR-155 can be used to predict the clinical characteristics of patients with LSCC and correctly diagnose them. Materials and Methods: We collected tissue samples and peripheral blood samples before and after treatment from 280 LSCC cases and 560 controls. Real-time quantitative reverse transcription PCR was employed in this study to compare the relative expressionof miR-155. Results: A total of 280 LSCC patients and 560 age- and sex-matched controls were included in the study. The miR-155 level was more up-regulated in LSCC tissue than in the non-tumor tissues (13.6±2.4 vs. 3.1±0.80, p<0.001). Additionally, a significantly higher miR-155 level in plasma samples from LSCC patients than in those of the controls (8.9±1.25 vs. 1.8±0.8, p<0.001) was reported. Tissue miR-155 showed an area under the curve (AUC) of 0.933, with a sensitivity of 82.6% and a specificity of 89.2%. The AUC for plasma miR-155 was 0.757, with a sensitivity of 58.4% and a specificity of 69.5%. When early LSCC in TNM I stage was considered, tissue miR-155 showed an area under the curve of 0.804, with a sensitivity of 85.2% and a specificity of 87.3%. Conclusion: The expression of tissue and plasma miR-155 were significantly up-regulated in patients with LSCC. Our work will serve as a basis for further investigation, preferably large-scale validation in clinical trials.
Ginsenoside Rg3 ameliorates allergic airway infl ammation and oxidative stress in mice
Wen-Chung Huang,Tse-Hung Huang,Kuo-Wei Yeh,Ya-Ling Chen,Szu-Chuan Shen,Chian-Jiun Liou 고려인삼학회 2021 Journal of Ginseng Research Vol.45 No.6
Background: Ginsenoside Rg3, isolated from Panax ginseng, has anti-inflammatory and anti-tumor activities. It is known to reduce inflammation in acute lung injury in mice, and to reduce the expression ofinflammatory cytokines and COX-2 in human asthmatic airway epithelium. In this study, we attemptedto determine whether ginsenoside Rg3 inhibits airway inflammation, oxidative stress, and airwayhyperresponsiveness (AHR) in the lungs of asthmatic mice. We also investigated its effects on oxidativestress and the inflammatory response in tracheal epithelial cells. Methods: Asthma symptoms were induced in female BALB/c mice sensitized with ovalbumin (OVA). Micewere divided into five groups: normal controls, OVA-induced asthmatic controls, and asthmatic micetreated with ginsenoside Rg3 or prednisolone by intraperitoneal injection. Inflammatory BEAS-2B cells(human tracheal epithelial cells) treated with ginsenoside Rg3 to investigate its effects on inflammatorycytokines and oxidative responses. Results: Ginsenoside Rg3 treatment significantly reduced eosinophil infiltration, oxidative responses,airway inflammation, and AHR in the lungs of asthmatic mice. Ginsenoside Rg3 reduced Th2 cytokineand chemokine levels in bronchoalveolar lavage fluids and lung. Inflammatory BEAS-2B cells treated withginsenoside Rg3 reduced the eotaxin and pro-inflammatory cytokine expressions, and monocyteadherence to BEAS-2B cells was significantly reduced as a result of decreased ICAM-1 expression. Furthermore, ginsenoside Rg3 reduced the expression of reactive oxygen species in inflammatory BEAS-2B cells. Conclusion: Ginsenoside Rg3 is a potential immunomodulator that can ameliorate pathological featuresof asthma by decreasing oxidative stress and inflammation
Wen, Feng,Li, Qiang,Li, Rui,Liu, Ling,Wang, Tao,Liu, Li,Wu, Tao,Li, Yuxiao The Korean Institute of Power Electronics 2020 JOURNAL OF POWER ELECTRONICS Vol.20 No.1
This paper presents a new type of a transmitter coil for wireless power transfer (WPT) to assist in the achievement of a constant output power and in the reduction of magnetic field leakage under misaligned electric vehicle (EV) wireless-charging conditions. The proposed DDP coil is an improvement upon the conventional DD coil and can be adaptively reconfigured depending on the misalignment of different sizes or types of receiver coils. The reconfigurable resonant circuit is designed to compensate various coil structures and to maintain a high-energy efficiency. The reconfiguration strategy is presented by studying the characteristics of the magnetic field and mutual inductance of the coils. A precise human anatomic model and a full-scale Tesla EV model are built to evaluate the magnetic field leakage and human exposure to the WPT system. Simulation results show that the power transfer efficiency (PTE) reaches 99.16% and that the maximum electric field induced in humans is -1.11 dBV/m, while the transfer power is 6.6 kW with a 12 cm lateral offset on both the x-axis and y-axis. The effectiveness of the proposed type of transmitter coil is verified through simulation and experimental results.
( Wen Bing Wang ),( Ying Sun ),( Li Na Song ),( Yan Wu ),( Hui Ling Wu ) 한국잠사학회 2008 International Journal of Industrial Entomology Vol.17 No.1
The caspase family proteins play an important role in programmed cell death (apoptosis). To date, the expression profiles of the caspase family genes in Bombyx mori (Bm) are poorly known. In this study, we examined the expression profiles of two novel Bm caspase family genes (ice-2 and ice-5), the potential change of the mitochondrial membrane and the morphology in Bm cells after stimulation of ultraviolet (UV) irradiation. The results showed the potential change of the mitochondrial membrane occurred at 5 hours after UV irradiation treatment. Analysis of fluorescent quantitative RT-PCR demonstrated that both the ice-2 and ice-5 might be involved in UV induced apoptosis in Bm cells. Notably, after UV irradiating, expression pattern of ice-2 and ice-5 were remarkably different. The ice-2 gene was highly expressed at two time points, 0.5 and 5 hours after UV stimulating, while the expression level of ice-5 only peaked at 5 hours after UV stimulating. It indicated that apoptosis induced by UV irradiation was involved in the mitochondrial pathway and the two isoforms of Bm ice may act but play different role during the apoptosis of Bm cells.
A case study of damage detection in four-bays steel structures using the HHT approach
Wen-Ko Hsu,Cheng-Wu Chen,Dung-Jiang Chiou,Ming-Yi Liu,Wei-Ling Chiang,Pei-Chiung Huang 국제구조공학회 2014 Smart Structures and Systems, An International Jou Vol.14 No.4
This study aims to investigate the relationship between structural damage and sensitivity indices using the Hilbert-Huang transform (HHT) method. Two damage detection indices are proposed: the ratio of bandwidth (RB), and the ratio of effective stiffness (RES). The nonlinear four bays multiple degree of freedom models with various predominant frequencies are constructed using the SAP2000 program. Adjusted PGA earthquake data (Japan 311, Chi-Chi 921) are used as the excitations. Next, the damage detection indices obtained using the HHT and the fast Fourier transform (FFT) methods are evaluated based on the acceleration responses of the structures to earthquakes. Simulation results indicate that, the column of the 1st floor is the first yielding position and the RB value is changed when the RES<90% in all cases. Moreover, the RB value of the 1st floor changes more sensitive than those from the top floor. In addition, when the structural response is nonlinear (i.e., RES<100%), the RB and the RES curves indicate the incremental change in the HHT spectra. However, the same phenomenon can be found from FFT spectra only when the stiffness reduction is large enough. Therefore, the RB estimated from the smoothed HHT spectra is an effective and sensitive index for detecting structural damage.
Wen Ling Zhang,최형진 한국물리학회 2012 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.61 No.9
A suspension of semiconducting graphene oxide/polyaniline (GO/PANI) composite dispersed in silicone oil is considered as one of potential candidates for electrorheological (ER) fluids. The GO/PANI composite was synthesized by chemical oxidation polymerization of aniline in the presence of GO dispersion, in which the GO was prepared by a modified Hummers method. The characteristics of the composite were examined by SEM image and FT-IR spectra, indicating the coexistence of GO and PANI in the composite. The GO/PANI composite based ER fluid was prepared by dispersing the fabricated particles in silicone oil, and its dynamic viscoelastic properties were measured using a rotational rheometer under different electric field, showing that the ER fluid exhibited typical solid-like ER behaviors.