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Thi Khoa My Nguyen,기미란,손려강,김경희,홍정화,백승필 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.101 No.-
Silica-forming peptides (SFPs) and the SFP-modified structural proteins can be used as templates for thesynthesis of biosilica nanoparticles (NPs). However, such biomolecule-mediated synthesis showed limitationsfor the generation of NPs with sizes sub-50 nm. In this study, the SFP sequences(KPSHHHHHTGAN and KPTHHHHHHDG for Kps and Kpt, respectively) were fused to the C-terminus ofthe human ferritin heavy chain (Fn), resulting in the SFP moieties in the inner space of Fn (termed FncKpsand Fn-cKpt). Using Fn-cSFP templates for silicification in two-phase system, the Fn-cKps@SiO2and Fn-cKpt@SiO2 NPs were generated in mean diameters of 26 and 28 nm, respectively. Also, weemployed biosilica NPs sub-50 nm for a doxorubicin (Dox) delivery system (application model). FncKpt@SiO2 NPs exhibited a high loading efficiency compared to Fn-cKpt only (1.7-fold) and prolongedrelease patterns with Dox. Most importantly, the uptake of Fn-cKpt@SiO2 into cancer cells was increasedso that the efficient delivery of Dox to the cell inside was observed. The uniform generation of biosilicaNPs sub-50 nm obtained here is a new achievement. Together with the ideal pH-dependent drug release,the size-controlled design of biosilica will be a useful strategy for efficient delivery of chemical drugs totarget cells.
Thi Khoa My NGUYEN,Mi Ran KI,Ryeo Gang SON,Kyung Hee KIM,Jin Woo SHIN,Seung Pil PACK 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Silica nanoparticles (NPs) can be fabricated by silica-forming peptides (SFPs). In our previous reports, SFP was fused to N-terminus of ferritin (Fn) and SFP-displayed Fn cage was used as a template for the fabirication of biosilica NPs. Production of size- and shape-tunable silica NPs based on chimeric Fn has some limitations. Here, heavy chain of human Fn was genetically modified to fuse an SFP sequence (KPSHHHHHTGAN and KPTHHHHHHDG for Kps and Kpt, respectively) to C-terminus of Fn, resulting in presenting SFP moieties inner surface of Fn (termed Fn-cKps and Fn-cKpt). Using of Fn-cSFP templates in silicification, we obtained Fn-cKps@SiO₂ and Fn-cKpt@SiO₂ below 50 ㎚. In addition, we investigated that the loading efficiency of the Fn-cKpt@SiO₂ NPs was remarkably higher than that of Fn-cKpt only (1.7- fold). Dox release from the Dox-loadedFn-cKpt@SiO₂ NPs was prolonged and dependent on the pH conditions. The Fn-cKpt@SiO₂ NPs contained drug at neutral pH and released a large amount at low pH that may be an ideal property of drug carriers.
Thi Khoa My Nguyen,기미란,이창수,백승필 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.73 No.-
Biosilica materials can be generated by biomolecules under physiological or mild/ambient conditions. However, nanosized and tunable design method of biosilica particles has not yet been set up. Here, Kps, asilica forming peptide (KPSHHHHHTGAN) was introduced to N-terminus of ferritin via protein fusionmethod to generate new Kps-modified ferritin (Kps-Fn) for reliable formation of biosilica particles. Then,novel chimeric Kps-Fn was designed for controllable generation of biosilica nanoparticles (NPs). Bychanging the ratios of Kps-Fn and Fn subunits in the chimeric Kps-Fn templates, desired size of biosilicaNPs (100–500 nm) can be achieved. The low surface density of Kps on the chimeric template could lead tothe small-sized biosilica NPs with the increased surface area/amount. The biosilica NPs developed hereshowed better DNA adsorption/elution performance than the commercially available silica NPs. Thiswork is a new way to generate desirable biosilica NPs, which can be applied for the design of variousbiohybrid nanomaterials.
Biosilica-enveloped ferritin cage for more efficient drug deliveries
Ki, Mi-Ran,Nguyen, Thi Khoa My,Jun, Hyun Sik,Pack, Seung Pil Elsevier 2018 PROCESS BIOCHEMISTRY Vol.68 No.-
<P><B>Abstract</B></P> <P>Ferritin (FT), a cage-protein composed of 24 subunits, may carry small molecules inside the core of cage. To enhance the potential of FT as a more functional drug carrier, FT genetically-fused with silaffin R5 peptide at <I>N</I>-terminus (R5FT) and silica-enveloped cage protein (SiO<SUB>2</SUB>/R5FT) were developed. Doxorubicin (Dox) can be loaded into cage interior by re-assembly of R5FT. The release of Dox loaded in internal cages was retarded by the silica-coated matrix and showed the pH-controlled behavior, in which the release amount is larger in acidic environments than neutral. R5FT provides a biocompatible and eco-friendly manufacturing process for the drug-laden silica nanoparticles over conventional production methods.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Biosilica-enveloped R5-modified ferritin (SiO<SUB>2</SUB>/R5FT) was designed as novel drug carriers. </LI> <LI> Dox was loaded into interior cage by re-assembly of R5FT and enveloped by R5-mediated biosilicification. </LI> <LI> Dox loaded in SiO<SUB>2</SUB>/R5FT displayed sustained release, the patterns of which can be controlled by pH (7–5). </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>