응고제가 우유두부의 물성 및 관능적 특성에 미치는 영향

우나리야,이민선,박수진,강명화 동아시아식생활학회 2004 동아시아식생활학회지 Vol.14 No.5

Different types of coagulant and characteristics of its concentration added in the process of making milk curd were compared in this study. The pH of whey decreased when the amount of coagulant increased. The turbidity of whey was decreased when 5% of acetic acid and lemon juice were put into as coagulants. The texture of milk curd made with cow's milk and skim milk was measured the highest when acetic acid and lemon juice were added at 3, 5% of their concentration. The fracturability of milk curd made only with cow's milk decreased as the concentration of each coagulant increased. The cohesiveness was decreased as concentration of coagulant increased. The springiness was slightly changed depending on its coagulant but didn't show much of difference. The gummminess of milk curd made with cow's milk was increased when 3, 5% of coagulant was added. The result of sensory evaluation of milk curd showed that preference of milk curd wasn't depending on types of milk nor its coagulant. Also, flavor preference showed better when lemon juice was added. Preference in texture of milk curd was the highest made with cow's milk and skim milk. Preference in taste was high when 10% of lemon juice was added to skim milk and preference in its appearance showed higher when the alum was added as a coagulant to both cow's milk and skim milk than other coagulants.

Cone-beam CT를 이용한 골격성 III급 부정교합자의 하악골 후퇴술 후 상기도 변화에 관한 연구

김나리,김용일,박수병,황대석 대한치과교정학회 2010 대한치과교정학회지 Vol.40 No.3

악교정 수술은 안면골격형태 뿐만 아니라 상기도 공간에도 영향을 준다. 본 연구는 골격성 III급 부정교합자 중 하악골 후퇴술을 시행 받은 환자를 대상으로 상기도 공간의 부피변화를 관찰하기 위하여 시행되었다. 기존의 연구들이 측모두부방사선사진을 중심으로 시행하였으나 본 연구에서는 3차원 cone-beam computed tomography (CBCT)를 이용하여 영상을 재구성한 뒤 분석하였다. 연구 대상은 하악골 후퇴술을 시행 받은 20명(남성 12, 여성 8)이었으며, 술 전 평균 1.8주(Baseline), 술 후 평균 2.3개월(T1) 그리고 술 후 평균 1년(T2) 시기에 CBCT를 촬영하였다. 상기도 공간은 기준평면에 따라 비인두, 구인두, 하인두로 나누어 계측하고 Baseline, T1, T2를 각각 비교하였다. 결과로 술후 2.3개월(T1)시기에 상기도 공간은 상당히 감소하였으며 (p < 0.001), 술 후 1년 후(T2)에도 감소된 양은 증가하지 않았다. 구인두는 상기도 공간 중 가장 많은 감소폭을 보였다. 이러한 결과로 하악골후퇴술은 상기도 공간을 장·단기간동안 감소하는 것으로 나타났다. Objective: Lateral cephalometric radiographs have been the main form of resource for assessing two dimensional anteroposterior airway changes. The purpose of this study was to evaluate the three dimensional volumetric change in the upper airway space in Class III malocclusion patients who underwent mandibular setback surgery. Methods: Three dimensional cone-beam computed tomographs (CBCT) and their three dimensional reconstruction images were analyzed. The samples consisted of 20 adult patients (12 males and 8 females) who were diagnosed as skeletal Class III and underwent mandibular setback surgery. CBCTs were taken at 3 stages - Baseline (1.8 weeks before surgery), T1 (2.3 months after surgery), and T2 (1 year after surgery). Pharyngeal airway was separated according to the reference planes and reconstructed into the nasopharynx, the oropharynx and the hypopharynx. Measurements at Baseline, T1, and T2 were compared between groups. Results: The result showed the volume of the pharyngeal airway decreased significantly 2.3 months after surgery (p < 0.001) and the diminished airway did not recover after 1 year post-surgery. The oropharynx was the most decreased area. Conclusions: These findings suggest that mandibular setback surgery causes both short-term and long-term decrease in the upper airway space.

Morphological and molecular finding of Longicollum pagrosomi (Acanthocephala: Pomphorhynchidae) in cultured red sea bream from Korea

( Na-ri Ha ),( Eui-ju Hong ),( Si-yun Ryu ),( Cheolho Sim ),( Joon-seok Chae ),( Hyeon-cheol Kim ),( Jinho Park ),( Kyoung-seong Choi ),( Do-hyeon Yu ),( Bae-keun Park ) 한국가축위생학회 2017 韓國家畜衛生學會誌 Vol.40 No.3

Acanthocephalan worms were harvested from the posterior intestines of the cultured marine fishes from January to July 2016 in a fish market located in Daejeon metropolitan city, Republic of Korea. Totally 450 cultured fishes (rock fish 100, olive flounder 250, red sea bream 100) were surveyed. Of the 100 red sea bream fish, 37 fishes (37%) were diagnosed as positive for Longicollum pagrosomi by light, electron microscopic and molecular examination. The number of worm was 25∼78 (mean 51±13). However, we can`t found any worm from the cultured rock fish and olive flounder. After sequencing, none of Pomphorhynchidae family are not identical based on 18S rRNA gene, and this data were identified with the first report for 18S rRNA gene sequence of L. pagrosomi. Furthermore, we confirmed that L. pagrosomi of the cultured red sea bream in Republic of Korea is very common parasite.

MiR-23b-3p Suppresses Migration, Invasion and EMT by Downregulating CD44 in Hepatocellular Carcinoma

( Na Ri Park ),( Jung Hoon Cha ),( Sung Woo Cho ),( Pil Soo Sung ),( Jeong Won Jang ),( Jong Young Choi ),( Seung Kew Yoon ),( Si Hyun Bae ) 대한간학회 2020 춘·추계 학술대회 (KASL) Vol.2020 No.1

Aims: Cancer stem cells (CSC) play a key role in cancer invasion and metastasis. Among CSCs, CD44 has known as important modulators of epithelial-mesenchymal transition (EMT) together with transforming growth factor beta1 (TGF-ß1). EMT is epithelial cells lose their polarity and acquire mesenchymal cell migratory characteristics. miRNA could lead to either EMT through the regulation of various transcription factors. This study aimed to investigate the role of miR-23b-3p regulating the EMT, migration and invasion as well as CD44 expression in HCC cell lines. Methods: We induced EMT by TGF-ß1 treatment or inhibited EMT by TGF-ß1 inhibitors. Also, miR-23b-3p mimic and inhibitor were transfected into HCC cell lines. The expression of EMT-related mRNA and protein were detected by quantitative real-time PCR and western blot. Also, EMT characteristics analyzed with cell migration and invasion. Results: FACS analysis showed high expression of CD44 in two HCC cell lines with different levels of TGF-ß1 expression. TGF-ß1-negative SNU-354 cells were treated with TGF-ß1 to induce the EMT and TGF-ß1-positive SNU-368 cells were treated with TGF-ß1 inhibitor to induce the MET. The expression of miR-23b-3p was down-regulated during the EMT and up-regulated during the MET. The Inhibition of miR-23b-3p in SNU-354 cells promoted EMT, cell migration and invasion. In contrast, overexpression of miR-23b-3p in SNU-368 cells suppressed EMT, cell migration and invasion. In addition, TGF-ß1 stimulation after miR-23b-3p overexpression induced neither the mesenchymal phenotype nor cell migration. Also, CD44 is a target of miR-23b-3p. CD44 expression was increased in miR- 23b-3p inhibitor cells, whereas miR-23b-3p overexpression cells reduced expression of the CD44 in HCC cells. Conclusions: Overexpression of miR-23b-3p suppressed EMT, cell migration and invasion by targeting CD44. The results suggest that miR-23b-3p may serve as specific biomarkers and therapeutic targets for HCC.

Synergistic Effect of CD44 and TGF-β1 during Epithelial- mesenchymal Transition through AKT/GSK3β/β-catenin Signaling

( Na Ri Park ),( Jung Hoon Cha ),( Jeong Won Jang ),( Jong Young Choi ),( Seung Kew Yoon ),( Si Hyun Bae ) 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1

Aims: The character of metastatic cells is strongly correlated to epithelial- mesenchymal transition (EMT) and cell adhesion molecules such as cadherin and CD44. CD44 is a receptor for hyaluronic acid, plays a role in migration, metastasis, and invasion. Moreover, transforming growth factor beta (TGF-β) signaling acts as the main factor in EMT. We investigate the correlation between high CD44 and TGF-β1 during EMT in HCC cell lines. Methods: We determined the expression of CD44 by FACS and expression of TGF-β1 from the cell supernatant by ELISA. To investigate synergy effect of CD44 and TGF-β1, we induced EMT by TGF-β1 treatment. Also, we inhibited EMT by shCD44 and TGF-β1 inhibitors. Morphological changes were evaluated using microscopy and expression of EMT-related proteins detected by western blot. Also, EMT characteristics analyzed with sphere formation and migration assay. Results: At the FACS analysis, the CD44 was highly expressed in SNU-354 and SNU-368 cell lines. TGF-β1 was only expressed in SNU-368 but not in SNU-354. SNU-368 CD44+ cells show EMT through up-regulation of AKT/GSK3β/β-catenin pathway. By comparison, SNU-354 CD44+ cells increased expression of N-cadherin but did not decrease the expression of E-cadherin, and then AKT/GSK3β pathway showed down-regulation. But, TGF-β1-treated SNU-354 cells exhibited morphological changes and accompanied by loss of E-cadherin and gain of N-cadherin with increased AKT/GSK3β/β-catenin. Also, TGF-β1-treated SNU-354 cells enhanced sphere formation and migration. On the other hand, TGF-β1-inhibited SNU-368 cells showed reduced N-cadherin and AKT/GSK3β/β -catenin. Also, TGF-β1 inhibition decreased sphere formation and migration. Moreover, the treatment with both shCD44 and TGF-β1-inhibitors reduced N-cadherin and AKT/β-catenin pathway and decreased migration in SNU-368 cells. Conclusions: TGF-β1 increased the expression of EMT-related proteins with CD44 in SNU-354 cells. TGF-β inhibitors showed reversed EMT in SNU-368. In addition, co-expression of TGF-β1 and CD44 were needed for tumor metastasis because it significantly increased sphere formation and migration.

New Records of Host Plant for Radopholus similis Isolated from Agathis dammara

Na-Ri Lee,Dong Woo Kim,Bo-ram Kim,Bok-ri Park,Jae yong Chun 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.04

The aim of the study is to report Radopholus similis detected from Agathis dammara in Thailand as new host plant. Existing host of R. similis was known as coffee, pepper, sugarcane and banana etc. This nematode in this study was observed morphologic character using Carl Zeiss Axioimager M2 and Axiovision Rel. 4.8 program. The result shows that a and b of the female was distorted toward maximum value of original description of species, and b′, c and stylet length was distorted toward minimum value of original description of species. This nematode was morphologically distinguished from originally detected R. similis, but measured value was similar in range of original description of species. For more information, molecular assay was also confirmed the R. similis with 98% homology with the sequence of the internal transcribed spacer region (ITS) of ribosomal DNA. We provided PCR-amplified ITS nucleotide sequence.

Epigenetic Regulation in the Brain after Spinal Cord Injury : A Comparative Study

Park, Bit-Na-Ri,Kim, Seok Won,Cho, Sung-Rae,Lee, Ji Yong,Lee, Young-Hee,Kim, Sung-Hoon The Korean Neurosurgical Society 2013 Journal of Korean neurosurgical society Vol.53 No.6

Objective : After spinal cord injury (SCI), functional and structural reorganization occurs at multiple levels of brain including motor cortex. However, the underlying mechanism still remains unclear. The current study was performed to investigate the alterations in the expression of the main regulators of neuronal development, survival and death, in the brain following thoracic contusive SCI in a mouse model. Methods : Eight-week-old female imprinting control region mice (n=60; 30-35 g) were used in this study. We analyzed the expression levels of regulators such as brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), nerve growth factor (NGF) and histone deacetylase (HDAC) 1 in the brain following thoracic contusive SCI. Results : The expression of BDNF levels were elevated significantly compared with control group at 2 weeks after injury (p<0.05). The expression of NGF levels were elevated at 2, 4 weeks compared with control group, but these difference were not significant (p>0.05). The GDNF levels were elevated at 2 week compared with control group, but these differences were not significant (p>0.05). The difference of HDAC1 levels were not significant at 2, 4 and 8 weeks compared with control group (p>0.05). Conclusion : These results demonstrate that the upregulation of BDNF may play on important role in brain reorganization after SCI.

24-hour Report on the 5th Collaborative Materials Exercise (CMX-5) for Nuclear Forensic Analysis

Na-Ri Lee,Jinkyu Park,Ranhee Park,Sun-Ho Han,Sang Ho Lim,Chi-Gyu Lee 한국방사성폐기물학회 2019 한국방사성폐기물학회 학술논문요약집 Vol.2019 No.10

황기 싹 및 잎 추출물의 랫드를 이용한 90일 반복경구투여 독성시험

최리나(Ri Na Choi),박해일(Hae il park),심훈섭(Hun Seob Shim),임정대(Jung Dae Lim),유창연(Chang Yeon Yu),최대성(Dae-Sung Cheoi),최유순(Yu-Soon Cheoi),정일민(Ill-Min Chung),박영철(Young Cheol Park) 한국약용작물학회 2013 한국약용작물학회 학술대회논문집 Vol.2013 No.1

하수처리시설에서 인 회수공정의 도입 가능성에 대한 실증적 검토

박나리(Na-ri Park),장향연(Hyang-Youn Chang),임현만(Hyun-Man Lim),안광호(Kwang-Ho Ahn),김원재(Weon-Jae Kim) 大韓環境工學會 2017 대한환경공학회지 Vol.39 No.1

본 연구에서는 경기도 고양시 소재 I 수질복원센터의 공정별 유량과 총인(TP) 농도를 활용하여 2015년도 기준 TP 물질수지도를 작성하고, 이를 분석하여 하수처리시설에서 인(P)을 회수할 수 있는 최적의 단위공정을 실증적으로 선정하고자 하였다. 인 회수공정의 도입 가능성을 평가하기 위하여 유량, TP농도, TP부하량 및 SS에 대한 정량적 지표를 제안하는 한편, 이를 I 수질복원센터에 적용하였다. 또한 I 수질복원센터의 탈수여액을 대상으로 인 회수공정의 도입 가능성에 대한 기초실험을 실시하였다. TP 물질수지를 분석한 결과, 하수처리장 유입수에 비해 유량은 1/16로 적지만 TP농도는 약 78배, TP부하량은 4.8배에 달하는 반류수 계통을 대상으로 인을 회수하는 것이 하수처리시설 전체의 TP부하를 저감하는데 유효한 것으로 나타났다. 반류수 계통의 단위공정들을 대상으로 본 연구에서 제안한 정량적 지표를 활용하여 인 회수공정의 도입 가능성을 상세히 평가한 결과, (잉여농축조 상징액+잉여저류조 월류수) 또는 탈수여액이 적합한 것으로 나타났다. 한편, TP농도가 141.5 mg/L에 달하는 탈수여액을 대상으로 결정화에 의한 인 회수 기초실험을 실시하였다. 탈수여액의 pH를 10으로 조정한 후 칼슘이온(Ca<SUP>2+</SUP>)을 각각 250, 500, 1000 mg/L씩 주입하여 완속교반을 실시하였다. 기초실험 결과 탈수여액 중의 PO₄-P 및 TP를 95% 이상 제거할 수 있는 것으로 나타나 인 회수공정의 도입 가능성이 확인되었다. In this study, we have made the annual total phosphorus (TP) mass balance diagrams for I wastewater treatment plant by utilizing the data of flow rate and TP of each process and tried to choose the optimum unit process empirically for phosphorus recovery. For the applicability evaluation, we have suggested several quantitative indices of flow rate, TP concentration, TP load and SS. Based on the analyses of TP mass balance, it became clear for reducing TP load of the wastewater treatment plant that it is efficient to recover phosphorus from the side stream in which the amount of flow rate is just 1/16, but TP concentration and load are 78 and 4.8 times larger than those of the influent of the plant. After the detailed applicability evaluation for the side stream, it could be concluded that the unit process of waste activated sludge thickener supernatant or dehydration filtrate are appropriate. Meanwhile, we did fundamental experiments utilizing the dewatering filtrate with TP concentration of 141.5 mg/L. After adjusting pH 10 and Ca<SUP>2+</SUP> concentration 250, 500, 1000 mg/L, it was stirred slowly. As a result, the PO₄-P and TP removal efficiencies were above 95 percent; the results of the experiment imply the applicability of phosphorus recovery process in a wastewater treatment plant strongly.