In Vivo Chemosenitivity Assay on the Subrenal Capsular Xenografts

Han, Ku Taek,Kim, Seung Jo,Namkoong, Sung Eun,Lee, Hun Young CATHOLIC MEDICAL CENTER 1988 Bulletin of the Clinical Research Institute Vol.16/17 No.1

The subrenal capsular assay is an in vivo method which permits chemosensitivity assessment in the human tumor fragments implanted into the sburenal capsular (SRC) space of mice. Previous studies have used transplantation-established human tumor xenograft systems implanted into athymic nude as well as normal immunocompetent mice. More recently, the subrenal capsular assay has been used to test of effectiveness of anticancer drugs against tumor xenograft implanted into normal immunocompetent mice using the 4 or 6 day time frame assay methods. On day 4, mice were sacrificed and the mean tumor diameter was measured. The tumor bearing kidney was fixed in 10% formalin solution and stained with hematoxylin-eosin. Histological analysis was performed by calculating their histologic scores which include the changes of amount and the extents of preservation of cancer cells, numbers of mitoses, and extents of tumor cell necroses or degenerations. We compared the changes of tumor size with histologic scores in the cervical cancer, ovarian cancer, and choriocarcinoma xenografts after the administration of the anticancer drugs and concluded as follows: 1. There were no statistically significant differences of the tumor size when compared that of the floated group with that of xenografts of the control group. 2. There were statistically significant differences of the histological scores when compared those of the treated group with those of xenografts of the control group. These results suggest that the analysis by calculating histological score of the xenografted tumor is more reliable than the simple measurement of tumor size change for the evaluation of the chemosensitivity of anticancer drugs in the 4-day in vivo chemosensitivity test.

-10℃ 조건에서의 열선 및 단열보온 조합양생에 따른 슬래브콘크리트의 온도이력

한상윤(Han, Sang Yoon),정상현(Cheong, Sang Hyun),안상구(Ahn, Sang Ku),고경택(Koh, Kyung Taek),한민철(Han, Min Cheol),한천구(Han, Cheon Goo) 한국콘크리트학회 2011 한국콘크리트학회 학술대회 논문집 Vol.2011 No.11

자궁경부 종양에서 p53, Proliferating Cell Nuclear Antigen(PCNA), c-myc 및 Epidermal Growth Factor Receptor(EGFR)의 발현

한구택 ( Ku Taek Han ),이형근 ( Hyung Kun Lee ),유순원 ( Sun Won Yoo ),송영훈 ( Young Hun Song ),최원영 ( Won Young Choi ),유기성 ( Ki Sung Ryu ),나종구 ( Jong Gu Rha ),송승규 ( Seung Kyu Song ),이헌영 ( Hun Young Lee ),김수평 ( S 대한산부인과학회 1997 Obstetrics & Gynecology Science Vol.40 No.12

Expression of four biologic markers known as p53, proliferating cell nuclear antigen(PCNA), c-myc, and epidermal growth factor receptor(EGFR), was studied immunohistochemically in 121 cases of cervical tissues, which included 15 cases of normal cervical tissues, 50 cases of squamous intraepithelial lesions(SIL), and 56 cases of invasive carcinomas. This study was designed to elucidate the roles of these factors in the genesis and progression of cervical neoplasia and to identify their association with clinical parameters such as cell type, tumor size, lymph node involvement, squamous cell carcinoma(SCC) antigen level, and prognosis. In additon, this study evaluates the differences of coexpression rates in normal cervical tissues, SILs, and invasive carcinomas. (1) The intensities of p53, c-myc, and EGFR expression and the rates of PCNA, c-myc, and EGFR expression in cervical SILs and invasive carcinomas were significantly higher than those in normal tissues (p<0.05 and p<0.01). But there were no significant differences between SILs and invasive carcinomas in both of intensities and rates of p53, PCNA, c-myc, and EGFR expression. (2) The intensities and rates of p53, PCNA, and c-myc expression in the invasive carcinomas did not correlate with clinical parameters including cell types, tumor sizes, lymph node involvements, see antigen, and prognosis. But only the intensity and rate of EGFR expression were significantly higher in cases with tumor size larger than 3 cm when it was compared with cases with tumor sizes smaller than 3 cm(p<0.01), in cases with lymph node involvement when it was compared with cases without lymph node involvement(p<0.05), and in cases with SCC antigen levels more than 2.5 ng/ml when it was compared with cases with SCC antigen levels less than 2.5 ng/ml(p<0.05). (3) C-myc immunoreactivity was significantly correlated with EGFR overexpression(p<0.01). (4) The rates of simultaneous expression of four factors were decreased significantly in normal tissues and significantly increased in invasive carcinomas(p<0.01). The coexpression rate of two factors or more was 6.7% in normal tissues, 62.7% in SILs, and 70.0% in invasive carcinomas and the simultaneous expression rate of three factors or more was none in normal tissues, 34.0% in SILs, and 47.5 % in invasive carcinomas. These results show that the immunohistochemical detection of p53, PCNA, c-myc, and EGFR expression will be useful in differentiating the normal tissues and cervical neoplasia. The results of simultaneous immunohistochemical detection of these four factor expression also suggest to contribute a better understanding of the genesis of cervical carcinoma and that coexpression of more factors indicate more aggressiveness in cervical neoplasia, and show that simultaneous detection of these factors can be used in the early detection of cervical neoplasia and to predict malignant transformation of cervical lesions.

Incidence of Virus Diseases in Major Cultivated Areas of Watermelon and Melon in Chungbuk Province

Han Jong-Woo,Park Young-Uk,Youn Cheol-Ku,Lee Seok-Ho,Jeong Taek-Goo,Choi Hong-Soo,김미경 한국식물병리학회 2023 식물병연구 Vol.29 No.1

To investigate the incidence status of viruses in major cultivated areas of watermelon and melon in Chungbuk Province, samples were collected from 2020 to 2021 in vinyl greenhouse of Jincheon and Eumseong and examined for virus infection using reverse transcription polymerase chain reaction. Of the six viruses on watermelon that was analyzed in this study, watermelons were infected with cucumber mosaic virus (CMV), watermelon mosaic virus (WMV), cucumber green mottle mosaic virus (CGMMV), and cucurbit aphid-borne yellows virus (CABYV). The incidence rate of CMV was 20.9–35.0%, WMV 0.4–15.8%, CGMMV 1.6–38.5%, and CABYV was 3.5–3.7% from 2020 to 2021. But strangely, there were no incidence of zucchini yellow mosaic virus and cucurbit chlorotic yellows virus (CCYV) during investigation. From this result, we knew the major virus was CGMMV on watermelon in Chungbuk Province. Molecular diagnosis assays of the two melon viruses, showed that melons were infected with CABYV and CCYV from 2020 to 2021. The incidence rate of CABYV was 53.9–92.2% and CCYV was 2.7–20.8%. The incidence of CABYV was high in melon cultivation of Jincheon and Eumseong, Chungbuk. Afterwards, it is necessary to establish a control management strategy for reduce the incidence of CABYV. Furthermore, we must pay attention that of CCYV even if the incidence was low.

운동유발전위를 이용한 뇌내출혈 환자에서 운동기능 회복의 예측

한문구,이광훈,한현정,정중택,이상수,한설희 대한신경과학회 1996 대한신경과학회지 Vol.14 No.1

난소암에서 자가 골수이식과 말초혈액 지지에 의한 고용량 항암 화학요법

한구택 대한조혈모세포이식학회 1996 대한조혈모세포이식학회지 Vol.1 No.1

고형 종양 특허 난소암에서 고용량의 항암 화학요법의 시도는 전 세계적으로도 매우 적으나, 진행성 난소암에서 사용되어온 표준 항암 화학요법제의 약제반응과 장기간의 무병 생존율은 20% 정도이지만 내성을 일으켰거나 진행된 난소암 환자에서 현재 까지 시도된 자가 골수 이식 지지나 말초 혈액 선조세포 지지에 의한 고용량의 항암 화학요법의 결과들은 약 70-80%의 매우 높은 반응률을 보였다. 이러한 결과들은 고용량의 항암제와 골수 또는 말초 혈액 원조 세포 지지 요법에 대한 약속된 결과를 암시하는 것이다. 그러나 비록 종양의 용적의 감소는 나타났지만 9개월 정도로 보고 된 짧은 평균 반응 기간은 실제적으로는 잔존 종양이 남아있음을 암시한다. 따라서 질환의 경과 중에서 종양의 내성이 덜 나타나는 더 조기에 고용량의 화학요법을 적용한다면 좀 더 지속되는 항종양 효과를 얻을 수 있을 것이다. 그러나 자기골수이식 지지와 말초혈액 선조세포 지지에 의한 고요량 항암 화학요법은 이환율과 사망율의 관점에서 많은 비용을 필요로 하고 여러 전문분야(multidisciplinary effort)의 노력 이 필요하므로 일률적으로 치명적인 질환에서 평균 무재발 생존률 또는 전체 생존률의 연장의 시위보다는 더 나은 목표를 설정해야 하며 백혈병이나 림프종에서와 같이 많은 환자들에서 장기간 지속되는 무병생존(완처)의 성취가 궁국적인 목표이어야 할 것으로 생각되며 본 산부인과학 교실에서도 내과학 교실과 골수이식 센터의 도움으로 고요량 하암도법의 도입이 하루 빨리 이루어지기를 기대한다.

사과酒 製造過程中 Fusel Oil 含量 變化에 關한 硏究-第2報

韓榮求,洪鍾旭,鄭基澤,姜昌錫 慶北大學校 1987 農業科學技術硏究所報 Vol.4 No.-

사과주 製造過程中 fusel oil 含量 變化를 調査한 結果를 要約하면 다음과 같다. 醱酵過程中 n-PrOH 含量 變化는 品種間의 差異만 顯著하게 나타났고, 동일 品種에 있어서 溫度, 菌株間에는 거의 變化가 없었다. 醱酵過程中 n-BuOH 含量 變化는 品種, 溫度, 菌株間에 있어 變化가 거의 없었다. 醱酵過程中 iso-BuOH 含量 變化는 菌株217에 있어서 醱酵初期 66時間까지는 急激한 增加가 있었고, OC-2에 있어서는 漸進的인 增加가 있었다. 그 以後부터는 두 菌株에 있어서 거의 變化가 없었으며, 菌株間에 있어서는 217이 OC-2보다 2倍程度 많은 量이 生成되었다. 醱酵過程中 iso-AmOH 含量 變化는 菌株 217은 66時間, OC-2는 90時間까지 急激한 增加를 하였고, 그以後부터는 變化가 거의 없었으며, 菌株간에 있어서 217은 OC-2보다 2倍 程度 많은 量이 生成되었다. Apple wine 製造에 있어서 fusel oil 生成은 醱酵溫度, 品種보다는 使用한 菌株에 依해서 많은 影響을 받았다. This study was carried out to investigate the changes of fusel oil contents during apple wine making. The results were as follow: n-BuOH was not changed during fermentation but n-PrOH was remarkably different by varieties of apple and was somewhat increased to 18 hrs fermentation. iso-BuOH was rapidly increased to 66 hrs fermentation by S. cerevisiae 217 and gradually increased by S. cerevisiae OC-2. After those times both S. cerevisiae 217 and OC-2 were gradually increased. The amounts of iso-BuOH produced by S. cerevisiae 217 were twice than those of OC-2. iso-AmOH was rapidly increased to 66 hrs fermentation by S. cerevisiae 217 and 90 hrs by OC-2. After those times iso-AmOH was gradually increased. The amounts of iso-AmOH produced by S. cerevisiae 217 were twice than those of OC-2. During apple wine making the production of fusel oil was more effected by the yeast stains than by the temperatures of fermentation and by the varieties of apple.

Effects of Morphology on the Electrical and Mechanical Properties of the Polycarbonate/Multi-Walled Carbon Nanotube Composites

Chong Ku Kum,Yu Taek Sung,Mi Sun Han,Woo Nyon Kim,Heon Sang Lee,Sun Jeong Lee,Jin Soo Joo 한국고분자학회 2006 Macromolecular Research Vol.14 No.4

Effect of Ridge-up Bed Cultivation and Stem Training Method on the Plant Growth and Yield of Cynanachum willfordii

Cheol Ku Youn(윤철구),Ki Hyun Kim(김기현),Dong Ho Han(한동호),In Jae Kim(김인재),Hee Do Lee(이희두),Seong Taek Hong(홍성택),Eui Yon Hong(홍의연),Young Guk Kim(김영국) 한국약용작물학회 2016 한국약용작물학회 학술대회논문집 Vol.2016 No.1

고휴재배 및 유인방법이 백수오 수량 및 생육에 미치는 영향

Cheol Ku Youn,Ki Hyun Kim,Dong Ho Han,In Jae Kim,Hee Do Lee,Seong Taek Hong,Eui Yon Hong,Young Guk Kim 한국약용작물학회 2016 한국약용작물학술대회 발표집 Vol.2016 No.05

Background : The effect of ridge-up bed cultivation and stem training method on the plant growth and yield of Cynanachum willfordii was investigated. Methods and Results : Domestic variety of Cynanachum willfordii was tested at Chungbuk ARES under the following conditions. Nonsupporting (custom) and I-form supporting was used for stem training method, and ridge height was set as 30 (custom) and 60 cm (high ridge). Planting spacing was adjusted to 30 (interrow spacing) × 20 cm (intrarow spacing), and other major management was followed the method of standard cultivation for medicinal crop. Investigated result from leaf characteristics, leaf length was longer in high ridge cultivation (HRC) as 11.1 cm than custom cultivation (CC) as 10.6 cm. Leaf width is proved to be 12.8 (HRC) > 11.2 cm (CC). Leaf number is proved to be 294 (HRC) > 254/plant (CC), with higher number of 44/plant at HRC and weight/10 leaves were more heavy at HRC (4.9 g) than that of CC (2.6 g). It was more fruit setting at HRC over 15/plant. According to the stem training method and ridge height from nonsupporting cultivation, main root was 4.0 (CC), higher than that of HRC over 0.5/plant. However, root length was more longer in HRC (28.6) than that of CC (25.0 cm). Main root diameter was also showed more thicker pattern in HRC. From staking cultivation, root number of HRC was 7/plant, it was recorded more 3/plant than that of CC, and it was also same pattern in main root length and root diameter. By the standard of commercial root, yield of living roots in nonsupporting cultivation were 59.0g/plant (HRC), it was more heavy over 10.4g/plant than that of CC. In staking cultivation, HRC were recorded as 74.2g/plant, more heavy 6.9g/plant than that of CC. Yield from I-form support stem training and ridge-up bed cultivation of HRC was higher approxmiately 52.7% than that of CC. Conclusion : Overall growth by high ridge cultivation of I-form support was good and yield of HRC was also increased over 52.7% than that of CC.