Effects of astaxanthin on antioxidant capacity of golden pompano (Trachinotus ovatus) in vivo and in vitro

( Jia-jun Xie ),( Xu Chen ),( Jin Niu ),( Jun Wang ),( Yun Wang ),( Qiang-qiang Liu ) 한국수산과학회 2017 Fisheries and Aquatic Sciences Vol.20 No.2

The objective of this research was to study the effect of astaxanthin (AST) on growth performance and antioxidant capacity in golden pompano (Trachinotus ovatus) both in vivo and in vitro. In the in vivo study, two diets were formulated with or without astaxanthin supplementation (D1 and D2; 0 and 200 mg/kg) to feed fish for 6 weeks. In the in vitro study, cells from hepatopancreas of golden pompano were isolated and four treatments with or without astaxanthin and H₂O₂ supplementation were applied (control group: without both astaxanthin and H₂O₂ treated; H₂O₂ group: just with H₂O₂ treated; H₂O₂ + AST group: with both astaxanthin and H₂O₂ treated; AST group: just with AST treated). Results of the in vivo study showed that weight gain (WG) and special growth rate (SGR) significantly increased with astaxanthin supplemented (P < 0.05). Feed conversion ratio (FCR) of fish fed D2 diet was significantly lower than that of fish fed D1 diet (P < 0.05). Hepatic total antioxidant capacity (T-AOC) and the reduced glutathione (GSH) of golden pompano fed D2 diet were significant higher than those of fish fed D1 diet (P < 0.05). Superoxide dismutase (SOD) was significantly declined as astaxanthin was supplemented (P < 0.05). Results of the in vitro study showed that the cell viability of H₂O₂ group was 52.37% compared to the control group, and it was significantly elevated to 84.18% by astaxanthin supplementation (H₂O₂ + AST group) (P < 0.05). The total antioxidant capacity (T-AOC) and the reduced glutathione (GSH) of cell were significant decreased by oxidative stress from H₂O₂ (P < 0.05), but it could be raised by astaxanthin supplementation (H₂O₂ vs H₂O₂ + AST), and the malondialdehyde (MDA) was significant higher in H₂O₂ group (P < 0.05) and astaxanthin supplementation could alleviate the cells from lipid peroxidation injury. In conclusion, dietary astaxanthin supplementation can improve the growth performance of golden pompano. Moreover, astaxanthin can improve the golden pompano hepatic antioxidant capacity both in vivo and in vitro study by eliminating the reactive oxygen species.

Effects of astaxanthin on antioxidant capacity of golden pompano (Trachinotus ovatus) in vivo and in vitro

Xie, Jia-jun,Chen, Xu,Niu, Jin,Wang, Jun,Wang, Yun,Liu, Qiang-qiang The Korean Society of Fisheries and Aquatic Scienc 2017 Fisheries and Aquatic Sciences Vol.20 No.4

The objective of this research was to study the effect of astaxanthin (AST) on growth performance and antioxidant capacity in golden pompano (Trachinotus ovatus) both in vivo and in vitro. In the in vivo study, two diets were formulated with or without astaxanthin supplementation (D1 and D2; 0 and 200 mg/kg) to feed fish for 6 weeks. In the in vitro study, cells from hepatopancreas of golden pompano were isolated and four treatments with or without astaxanthin and $H_2O_2$ supplementation were applied (control group: without both astaxanthin and $H_2O_2$ treated; $H_2O_2$ group: just with $H_2O_2$ treated; $H_2O_2$ + AST group: with both astaxanthin and $H_2O_2$treated; AST group: just with AST treated). Results of the in vivo study showed that weight gain (WG) and special growth rate (SGR) significantly increased with astaxanthin supplemented (P < 0.05). Feed conversion ratio (FCR) of fish fed D2 diet was significantly lower than that of fish fed D1 diet (P < 0.05). Hepatic total antioxidant capacity (T-AOC) and the reduced glutathione (GSH) of golden pompano fed D2 diet were significant higher than those of fish fed D1 diet (P < 0.05). Superoxide dismutase (SOD) was significantly declined as astaxanthin was supplemented (P < 0.05). Results of the in vitro study showed that the cell viability of $H_2O_2$ group was 52.37% compared to the control group, and it was significantly elevated to 84.18% by astaxanthin supplementation ($H_2O_2$ + AST group) (P < 0.05). The total antioxidant capacity (T-AOC) and the reduced glutathione (GSH) of cell were significant decreased by oxidative stress from $H_2O_2$ (P < 0.05), but it could be raised by astaxanthin supplementation ($H_2O_2$ vs $H_2O_2$ + AST), and the malondialdehyde (MDA) was significant higher in $H_2O_2$ group (P < 0.05) and astaxanthin supplementation could alleviate the cells from lipid peroxidation injury. In conclusion, dietary astaxanthin supplementation can improve the growth performance of golden pompano. Moreover, astaxanthin can improve the golden pompano hepatic antioxidant capacity both in vivo and in vitro study by eliminating the reactive oxygen species.

Optimum Design of Transverse Flux Machine for High Contribution of Permanent Magnet to Torque Using Response Surface Methodology

Jia Xie,Do-Hyun Kang,Byung-Chul Woo,Ji-Young Lee,Zheng-Hui Sha,Sheng-Dun Zhao 대한전기학회 2012 Journal of Electrical Engineering & Technology Vol.7 No.5

Transverse flux machine (TFM) has been proved to be very suitable for high-torque, lowspeed, and direct-drive situation in industry. But the complex structures and costly permanent magnets (PMs) are two key limitations of its wide range of applications. This paper proposes a new claw pole TFM (ACPTFM) which features an assembled claw pole stator and using the lamination steels material to overcome the complex structures. By combining response surface methodology (RSM) with design of experiment, an optimum design method is put forward to improve the PM’s contribution to the torque in order to save the PM’s amount. The optimum design results demonstrate the validity of the proposed optimum design method and the optimized model. Eventually, the finiteelement analysis (FEA) calculation method, which is used in the optimization process, is verified by the experiments in a prototype.

Efficient Certificateless Signature Scheme on NTRU Lattice

( Jia Xie ),( Yupu Hu ),( Juntao Gao ),( Wen Gao ),( Mingming Jiang ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.10

Because of the advantages of certificateless and no escrow feature over the regular signature and identity-based signature, certificateless signature has been widely applied in e-business, e-government and software security since it was proposed in 2003. Although a number of certificateless signature schemes have been proposed, there is only one lattice-based certificateless signature scheme which is still secure in the quantum era. But its efficiency is not very satisfactory. In this paper, the first certificateless signature scheme on NTRU lattice is proposed, which is proven to be secure in random oracle model. Moreover, the efficiency of the new scheme is higher than that of the only one lattice-based certificateless signature.

Optimum Design of Transverse Flux Machine for High Contribution of Permanent Magnet to Torque Using Response Surface Methodology

Xie, Jia,Kang, Do-Hyun,Woo, Byung-Chul,Lee, Ji-Young,Sha, Zheng-Hui,Zhao, Sheng-Dun The Korean Institute of Electrical Engineers 2012 Journal of Electrical Engineering & Technology Vol.7 No.5

Transverse flux machine (TFM) has been proved to be very suitable for high-torque, low-speed, and direct-drive situation in industry. But the complex structures and costly permanent magnets (PMs) are two key limitations of its wide range of applications. This paper proposes a new claw pole TFM (ACPTFM) which features an assembled claw pole stator and using the lamination steels material to overcome the complex structures. By combining response surface methodology (RSM) with design of experiment, an optimum design method is put forward to improve the PM's contribution to the torque in order to save the PM's amount. The optimum design results demonstrate the validity of the proposed optimum design method and the optimized model. Eventually, the finite-element analysis (FEA) calculation method, which is used in the optimization process, is verified by the experiments in a prototype.

Emodin Inhibits Breast Cancer Cell Proliferation through the ERα-MAPK/Akt-Cyclin D1/Bcl-2 Signaling Pathway

Sui, Jia-Qi,Xie, Kun-Peng,Zou, Wei,Xie, Ming-Jie Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15

Background: The aim of the present study was to investigate the involvement of emodin on the growth of human breast cancer MCF-7 and MDA-MB-231 cells and the estrogen (E2) signal pathway in vitro. Materials and Methods: MTT assays were used to detect the effects of emodin on E2 induced proliferation of MCF-7 and MDA-MB-231 cells. Flow cytometry (FCM) was applied to determine the effect of emodin on E2-induced apoptosis of MCF-7 cells. Western blotting allowed detection of the effects of emodin on the expression of estrogen receptor ${\alpha}$, cyclin D1 and B-cell lymphoma-2 (Bcl-2), mitogen-activated protein kinases (MAPK) and phosphatidylinostiol 3-kinases (PI3K). Luciferase assays were emplyed to assess transcriptional activity of $ER{\alpha}$. Results: Emodin could inhibit E2-induced MCF-7 cell proliferation and anti-apoptosis effects, and arrest the cell cycle in G0/G1 phase, further blocking the effect of E2 on expression and transcriptional activity of $ER{\alpha}$. Moreover, Emodin influenced the ER ${\alpha}$ genomic pathway via downregulation of cyclin D1 and Bcl-2 protein expression, and influenced the non-genomic pathway via decreased PI3K/Akt protein expression. Conclusions: These findings indicate that emodin exerts inhibitory effects on MCF-7 cell proliferation via inhibiting both non-genomic and genomic pathways.

Colorectal Cancer Mortality Characteristics and Predictions in China, 1991-2011

Fang, Jia-Ying,Dong, Hong-Li,Sang, Xue-Jin,Xie, Bin,Wu, Ku-Sheng,Du, Pei-Ling,Xu, Zhen-Xi,Jia, Xiao-Yue,Lin, Kun Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.17

Background: To identify the epidemiological characteristics of colorectal cancer mortality in China during the period of 1991-2011, and forecast the future five-year trend. Materials and Methods: Mortality data for colorectal cancer in China from 1991 to 2011 was used to describe epidemiological characteristics in terms of age group, gender, and rural/urban residence. Trend surface analysis was performed to analyze the geographical distribution of colorectal cancer. Four models including curve estimation, time series modeling, gray modeling and joinpoint regression were applied to forecast the trends for the future five years. Results: Since 1991 the colorectal cancer mortality rate increased yearly, and our results showed that the trend would continue to increase in the ensuing 5 years. The mortality rate in males was higher than that of females and the rate in urban areas was higher than in rural areas. The mortality rate was relatively low for individuals less than 60 years of age, but increased dramatically afterwards. People living in the northeastern China provinces or in eastern China had a higher mortality rate for colorectal cancer than those living in middle or western China provinces. Conclusions: The steadily increasing mortality of colorectal cancer in China will become a substantial public health burden in the foreseeable future. For this increasing trend to be controlled, further efforts should concentrate on educating the general public to increase prevention and early detection by screening. More effective prevention and management strategies are needed in higher mortality areas (Eastern parts of China) and high-risk populations (60+ years old).

Tensile behavior of stainless steel S30408 at the Arctic low temperature

Jia-Bao Yan,Zhicheng Lin,Peng Xie,Yun-Biao Luo,Yanbo Li 국제구조공학회 2021 Steel and Composite Structures, An International J Vol.40 No.5

This paper investigated tensile behaviours of S30408 at the Arctic low temperatures (T) of -80~20℃. A cooling chamber equipped with liquid nitrogen gas was adopted to rebuild the Arctic low-temperature environment. Standard tension tests were performed on 48 coupons with different thickness at different T values of -80, -60, -30, and 20℃. Test results revealed the ductile failure mode of stainless steel S30408 even at -80℃. The tensile stress-strain (σ-ε) curves of S30408 at ambient temperatures exhibited a long strain hardening plateau. Meanwhile, the low-temperature tensile σ-ε curves of S30408 were different from those at ambient temperatures, which exhibited a second hardening stage with a larger slope than the first hardening. The decreasing T generally increased the elastic modulus, yield and ultimate strength, but reduced the ductility of S30408. Decreasing the T from 20 to -80℃ reduced the ductility of S30408 by about 28%, but improved the elastic modulus, yield and ultimate strength by about 20%, 22%, and 75%, respectively. This paper also adopted the best subset regression analysis method to develop empirical formulae on estimating influences of T on mechanical properties of S30408. Validations of the predictions by these empirical formulae against the test results in the literature proved their accuracies.

Comparison of Liver Transplantation and Liver Resection for Hepatocellular Carcinoma Patients with Portal Vein Tumor Thrombus Type I and Type II

Jia-Yu Lv,Ning-Ning Zhang,Ya-Wei Du,Ying Wu,Tian-Qiang Song,Ya-Min Zhang,Yan Qu,Yu-Xin Liu,Jie Gu,Ze-Yu Wang,Yi-Bo Qiu,Bing Yang,Da-Zhi Tian,Qing-Jun Guo,Li Zhang,Ji-San Sun,Yan Xie,Zheng-Lu Wang,Xin 연세대학교의과대학 2021 Yonsei medical journal Vol.62 No.1

Purpose: The aim of this study was to compare the efficacy of liver transplantation (LT) and liver resection (LR) for hepatocellularcarcinoma (HCC) patients with portal vein tumor thrombus (PVTT) and to investigate risk factors affecting prognosis. Materials and Methods: A total of 94 HCC patients with PVTT type I (segmental PVTT) and PVTT type II (lobar PVTT) were involvedand divided into LR (n=47) and LT groups (n=47). Recurrence-free survival (RFS) and overall survival (OS) were comparedbefore and after inverse probability of treatment weighting (IPTW). Prognostic factors for RFS and OS were explored. Results: Two treatment groups were well-balanced using IPTW. In the entire cohort, LT provided a better prognosis than LR. Among patients with PVTT type I, RFS was better with LT (p=0.039); OS was not different significantly between LT and LR(p=0.093). In subgroup analysis of PVTT type I patients with α-fetoprotein (AFP) levels >200 ng/mL, LT elicited significantly longermedian RFS (18.0 months vs. 2.1 months, p=0.022) and relatively longer median OS time (23.6 months vs. 9.8 months, p=0.065). Among patients with PVTT type II, no significant differences in RFS and OS were found between LT and LR (p=0.115 and 0.335,respectively). Multivariate analyses showed treatment allocation (LR), tumor size (>5 cm), AFP and aspartate aminotransferase(AST) levels to be risk factors of RFS and treatment allocation (LR), AFP and AST as risk factors for OS. Conclusion: LT appeared to afford a better prognosis for HCC with PVTT type I than LR, especially in patients with AFP levels>200 ng/mL.

Research on the Power Sharing Control and Stability of VSGs

Xie, Dong,Zang, Da-Jin,Gao, Peng,Wang, Jun-Jia The Korean Institute of Power Electronics 2017 JOURNAL OF POWER ELECTRONICS Vol.17 No.2

Aiming at the deficiencies of power sharing control performances when a traditional droop control is adopted for microgrid inverters, this paper proposes a microgrid inverter power sharing control strategy based on a virtual synchronous generator. This control method simulates the electromechanical transient characteristics of a synchronous generator in a power system by an ontology algorithm and the control laws of a synchronous generator by control over the speed governor and excitation regulator. As a result, that the microgrid system is able to effectively retain the stability of the voltage and frequency, and the power sharing precision of the microgrid inverter is improved. Based on an analysis of stability of a microgrid system controlled by a virtual synchronous generator, design thoughts are provided for further improvement of the power sharing precision of inverters. The simulation results shows that when the virtual synchronous generator based control strategy was adopted, the power sharing performances of microgrid inverters are improved more obviously than those using the droop control strategy.