Impact of Irradiation Time on the Hydrolysis of Waste Activated Sludge by the Dielectric Heating of Microwave

Tae Joo Park,Im Gyu Byun,Jae Ho Lee,Ji Sung Lim,Jeong Min Lee 대한환경공학회 2014 Environmental Engineering Research Vol.19 No.1

The effects of initial solid concentration and microwave irradiation (MWI) time on the hydrolysis of waste activated sludge (WAS)were investigated. MWI time strongly influenced WAS hydrolysis for all initial solid concentrations of 8.20, 31.51, and 52.88 g VSS/L. For all WAS, the volatile suspended solids (VSS) solubilization degree ranged from 35.6% to 38.4% during a total MWI time of 10 min. Solublechemical oxygen demand (SCOD) concentration increased at a rate proportional to the decrease of VSS during the MWI. However, the clearly different VSS solubilization patterns that were observed during the MWI were explained by the 2-step hydrolysis of WAS, consisting of the initial disintegration of the easily degradable part of the sludge, followed by the subsequent disintegration of the hardlydegradable part of the sludge. WAS hydrolysis rates for 3 to 6 min of MWI were significantly lower than those for less than 3 min, or more than 6 min. From these results, 3 min MWI time and WAS of 31.51 g VSS/L (centrifugal thickener WAS) showed the most efficienthydrolysis of WAS at 36.0%. The profiles of total nitrogen (T-N) concentrations corresponded well to the SCOD increases in terms of the empirical formula of bacterial cell mass (C5H7O₂N). The negligible T-N increase and pH decrease during WAS hydrolysis by MWI will allowthe application of this process to subsequent biological processes, such as anaerobic digestion.

Highly mesoporous activated carbon fibers using high density polyethylene precursor with a multi-step stabilization technique for automobile carbon canister

Jeong-Rae Ahn,Hye-Min Lee,Byung-Joo Kim 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.128 No.-

In this study, activated carbon fibers (ACFs) for automobile carbon canisters were produced usinghigh-density polyethylene (HDPE) fibers treated with a multistep stabilization process as the precursor. The stabilization of the HDPE fibers involved the following sequential steps: (1) electron beamirradiation, (2) mild sulfonation, and (3) dry oxidation. The stabilized fibers were carbonized at 900℃in a nitrogen atmosphere. The carbonized fibers were activated at 900℃ in a water–vapor atmospherefrom 30-60 min. The textural properties of the activated carbon fibers were determined using N2/77 Kadsorption–desorption isotherms. The specific surface area ranged from 1090 to 1980 m2/g, and the totalpore volume ranged from 0.56 to 1.11 cm3/g. The specific surface area, micropore volume, and mesoporevolume increased with increasing activation time. The fractions of micropores and mesopores were similaracross all conditions, whereas the yield decreased gradually until 50 min, and then exhibited a sharpdecline at 60 min. The butane working capacity increased as much as 56.24% as the increase in activationtime. This can be attributed to the increase in butane activity owing to the increase in micropore volume,along with a decrease in butane resistivity caused by the increase in mesopore volume at a specificdiameter.

A Rapid and Sensitive Two-Site Sandwich Enzyme-Linked Immunosorbent Assay for Detection of ${\alpha}$-Fetoprotein in Human Serum

Jang, Jeong-Su,Kim, Jeong-Min,Chung, Gi-Hyun,Paik, Bo-Hyun,Kim, Hack-Joo Korean Society for Biochemistry and Molecular Biol 1996 Journal of biochemistry and molecular biology Vol.29 No.3

A rapid and sensitive method has been developed to detect a-fetoprotein (AFP) in human serum by a two-site sandwich enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies (MAbs) for human AFP within 1 h. To obtain the most sensitive and reliable MAbs. 12 kinds of MAbs (HPJ1 to HPJ12) as a capture antibody and 4 kinds of horseradish peroxidase (HRP) conjugated MAbs as a tracer antibody were investigated. Among these, only HPJ 10-HRP conjugated HPJ 1 (HPJ 10-HPJ $1^*$) and HPJ 11-HRP conjugated HPJ 10 (HPJ 11-HPJ $10^*$) were chosen as candidates based on the linearity of the standard curve and the sensitivity of the assay. To further characterize these two pairs. MAbs against human AFP were purified from hybridoma cells. conjugated with HRP. and then characterized to optimize the two-site sandwich ELISA The HPJ 10-HPJ $1^*$ pair showed a sensitivity of 1 ng/ml and a better reproducibility than the HPJ 11-HPJ $10^*$ pair when the human sera were incubated at $37^{\circ}C$ for 30 min. The results obtained for 480 randomly selected human sera showed 0~20 ng/ml of AFP values for the normal human sera. To test the utility of our kit, AFP concentrations were determined for 951 human sera (including 85 normal sera, 480 random blood sera, 213 HBsAg-positives. 50 anti-HCV antibody positives. and 47 malignant diseases) and compared with other commercially available AFP detecting kits. These results show that the present two-site sandwich ELISA method is a rapid, sensitive, and reliable procedure for detecting AFP in human serum.

하조대(강원도 양양군)의 거미상

김주필(Joo Pil Kim),예용택(Yong Taek Ye),신정애(Jeong Ae Sin),예성훈(Seong Hun Ye),성민규(Min Kyu Seong),이정준(Jeong Jun Lee),채준호(Jun Ho Chae) 한국거미연구소 2016 한국거미 Vol.32 No.1

2014년 10월 2일부터 4일까지 강원도 양양군 하조대 일대를 한국거미연구회 회원(김주필, 예용택, 신정애, 예성훈, 성민규, 이정준, 채준호)들이 조사 연구한 결과 11과 18속 20종이 채집되었기에 이에 보고하는 바이다. The authors investigated the spiders of the Hajodae beach (Gangwon-Do, Yangyang-Gun). In the present paper 20 species of 18 genera belong to 11 families were identified. These specimens are deposited at the Joo-Pil Spider Museum, Namyangju-Si, Gyeonggi-Do.

육백산(강원도, 삼척시)의 거미상

김주필(Joo Pil Kim),예용택(Yong Taek Ye),오종화(Jong Hwa Oh),예성훈(Seong Hun Ye),성민규(Min Gyu Seong),이정준(Jeong Jun Lee),김태우(Tae Woo Kim),김대희(Dae Hee Kim),지승환(Seung Hwan Ji),이형민(Hyeong Min Lee) 한국거미연구소 2016 한국거미 Vol.32 No.1

2015년 8월 3일부터 5일까지 한국거미연구회 회원들은 강원도 삼척시 육백산을 채집 조사한 결과 22과 49속 63종이 채집 조사되어 이에 보고하는 바이다. The authors investigated the spiders of the Mt. Yukbaek from August 3th to August 5th, 2015. In the present paper 63 species of 49 genera belong to 22 families were identified. These specimens are deposited at the Joo-Pil Spider Museum, Namyangju-Si, Gyeonggi-Do.

백서 척수후근절로부터 슈반세포의 효과적인 체외 배양법

김성민,이종호,김남열,안강민,최원재,최시호,차미주,이주영,황순정,장정원,명훈,최진영,서병무,정필훈,김명진 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.4

Schwann cells(SCs), an important component of the peripheral nervous system, intract with nerous to mutually support growth and replication for the peripheral nerve regentation. Recently, ading SCs to the lumen of guidance channel is widely tried to improve regeneration or to make regeneration possible over otherwise irreparable gaps. however, it is not easy to isolate and multiplicate SCs as much as enough to help the axonal regeneration. For the allogeneic SCs source for tubular nerve guidance, we developed a little bit improved technique of harvesting and multiplicating SCs. by culturing dispersed dorsal root ganglia in specially designed medium with growth factors and serial processing, we repeatedlly generate relatively homogenous SC cultures. Our technique was compared with other methods of literature using immunostaining methods such as GFAP, S100, BDNF and the total SC count assessment at different time interval after primary culture.

A Rapid and Sensitive Two - Site Sandwich Enzyme - Linked Immunosorbent Assay for Detection of α - Fetoprotein in Human Serum

Kim, Hack Joo,Kim, Jeong Min,Jang, Jeong Su,Paik, Bo Hyun,Chung, Gi Hyun 생화학분자생물학회 1981 BMB Reports Vol.29 No.3

A rapid and sensitive method has been developed to detect α-fetoprotein (AFP) in human serum by a two-site sandwich enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies (MAbs) for human AFP within 1 h. To obtain the most sensitive and reliable MAbs, 12 kinds of MAbs (HPJ1 to HPJ12) as a capture antibody and 4 kinds of horseradish peroxidase (HRP) conjugated MAbs as a tracer antibody were investigated. Among these, only HPJ 10-HRP conjugated HPJ 1 (HPJ 10-HPJ 1*) and HPJ 11-HRP conjugated HPJ 10 (HPJ 11-HPJ 10*) were chosen as candidates based on the linearity of the standard curve and the sensitivity of the assay. To further characterize these two pairs, MAbs against human AFP were purified from hybridoma cells, conjugated with HRP, and then characterized to optimize the two-site sandwich ELISA. The HPJ 10-HPJ 1 * pair showed a sensitivity of 1 ng/㎖ and a better reproducibility than the HPJ 11-HPJ 10* pair when the human sera were incubated at 37℃ for 30 min. The results obtained for 480 randomly selected human sera showed 0∼20 ng/㎖ of AFP values for the normal human sera. To test the utility of our kit, AFP concentrations were determined for 951 human sera (including 85 normal sera, 480 random blood sera, 213 HBsAg-positives, 50 anti-HCV antibody positives, and 47 malignant diseases) and compared with other commercially available AFP detecting kits. These results show that the present two-site sandwich ELISA method is a rapid, sensitive, and reliable procedure for detecting AFP in human serum.

Evaluation of 99mTc-MAG3-2-nitroimidazole for hypoxic tumor imaging

Yun Sang Lee,Young Joo Kim,Jae Min Jeong 대한방사성의약품학회 2019 Journal of radiopharmaceuticals and molecular prob Vol.5 No.1

2-Nitroimidazole derivatives have been reported to accumulate in hypoxic tissue. We prepared a novel 99mTc-MAG3-2-nitroimidazole and evaluated the feasibility for hypoxia imaging agent. Bz-MAG3-2-nitroimidazole wassynthesized by direct coupling of Bz-MAG3 and 2-nitroimidazole using dicyclohexylcarbodiimide. Bz-MAG3-2-nitroimidazole was labeled with 99mTc in the presence of tartaric acid and SnCl2-2H2O at 100°C for 30 min. And the reaction mixture was purified by C18 Sep-pak cartridge. The labeling efficiency and the radiochemicalpurity were checked by ITLC-SG/acetonitrile. The tumor was grown in balb/c mice for 8~13 days after thesubcutaneous injection of tumor cells, CT-26 (murine colon adenocarcinoma cell). Biodistribution study andtumor autoradiography were performed in the xenografted mice after i.v injection of 74 kBq/0.1 mL and 19MBq/0.1 mL of 99mTc-MAG3-2-nitroimidazole, respectively. In vivo images of 99mTc-MAG3-2-nitroimidazolein tumor bearing mice were obtained 1.5 hr post injection. The labeling efficiency was 45±20% and theradiochemical purity after purification was over 95%. Paper electrophoresis confirmed negative charge of99mTc-MAG3-2-nitroimidazole. 99mTc-MAG3-2-nitroimidazole was very stable at room temperature and its proteinbinding was 53%. The 99mTc-MAG3-2-nitroimidazole exhibited high uptake in the liver, stomach and intestine. In biodistribution study using tumor bearing mice, the uptakes (% ID/g) of the tumor were 0.5±0.1, 0.4±0.0,0.2±0.1 and 0.1±0.1 at 5, 15, 30 min and 4 hrs. Tumor/muscle ratio were 1.4±0.1, 2.2±0.83, 3.0±0.9, and 3.7(n=2) for 5, 15, 30 min and 4 hrs. The uptake in hypoxic area was found higher than in non-hypoxic area oftumor tissue by autoradiography. In vivo images showed the relatively faint uptake to the hypoxic tumor region. 99mTc-MAG3-2-nitroimidazole was successfully synthesized and found feasible for imaging hypoxia

Synthesis and biodistribution of 18F-labeled α-, β- and ω-fluorohexadecanoic acid

Yun-Sang Lee,Young Joo Kim,Gi Jeong Cheon,Jae Min Jeong 대한방사성의약품학회 2018 Journal of radiopharmaceuticals and molecular prob Vol.4 No.2

ω-[18F]-Fluorohexadecanoic acid (FHA) has been used for imaging of fatty acid metabolism of myocardium. To increase retention of radiolabeled fatty acid by blocking β-oxidation, methyl branched analogues have been used. In this experiment, we tried to synthesize 18F-labeled α-, β- and ω-FHA for imaging of the myocardial fatty acid metabolism. We synthesized α-, β- and ω-mesylated methyl hexadecanoates and labeled with 18F by reacting with [18F]TBAF in acetonitrile at 80ºC for 10 min. Methyl ester group was removed by 1 M NaOH at 80ºC for 5 min. The yields of α-[18F] and ω-[18F]FHA were 25.5 and 45.5%, respectively [EOS]. However, β-[18F] FHA was not labeled at all due to a fast elimination reaction. The biodistribution study in ICR-mice showed that ω-[18F]FHA has higher myocardial uptake and lower liver uptake than α-[18F]FHA. The electron-withdrawing effect of fluorine at α- position is believed to be the major factor affecting the biodistribution

허혈 유사 조건이 심방근의 활동전위 특성에 미치는 영향

김재하,주정민,조용범,신동호,안병희,주신배,김상형 대한순환기학회 1999 Korean Circulation Journal Vol.29 No.11