Phthalic Anhydride에 의한 직업성 천식

최승원 ( Seung Won Choi ),안종준 ( Jong Joon Ahn ),정현철 ( Hyun Chul Jung ),홍은석 ( Eun Seog Hong ),이지호 ( Ji Ho Lee ),김양호 ( Yang Ho Kim ) 대한천식알레르기학회 2005 천식 및 알레르기 Vol.25 No.4

Acid anhydrides, such as phthalic anhydride (PA) or trimellitic anhydride, are low molecular weight reactive chemicals that has been widely used in the manufacture of plastics and paints. They have been reported to cause rhinitis, dermatitis, asthma, and hemorrhagic alveolitis. These adverse effects are a result of direct irritation to the mucosa or its ability to cause immune reaction. We report a 32-year-old man with PA induced asthma, confirmed by specific inhalation challenge. He complained of recurrent chest tightness, sneezing, and dyspnea while at work. These symptoms develop 3 years after exposure to this chemical. Inhalation challenge with PA fume reproduced his symptoms and demonstrated an early asthmatic response. Specific IgE antibody against to PA was elevated. (Korean J Asthma Allergy Clin Immunol 2005;25:320-323)

Estimation of kinetic parameters for the reactor model of the phthalic anhydride production by the design of experiments

Pinthep Sethapokin,Siripoln Kunatippapong,Anchaleeporn Waritswat Lothongkum 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.24 No.-

Heterogeneous reactor model was developed to predict product, by-product and hot spot temperature in an industrial fixed bed reactor of phthalic anhydride production. This work modified the Calderbank’s kinetic model by including the reaction from o-xylene to by-product maleic anhydride. The kinetic parameters appropriating to the catalyst in this study were estimated by the design of experiments. The reactor model was validated by comparing the simulated results with the production data in terms of the outlet molar flow rates of phthalic anhydride and maleic anhydride, and the hot spot temperature. The results were less than 1.8% deviated

2-Aminobenzamide로부터 Quinazoline 4-one계 유도체의 합성(Ⅲ) : Acid anhydride와의 반응 Reaction with Acid Anhydrides

서명은 梨花女子大學校 藥學硏究所 1991 藥學硏究論文集 Vol.- No.1

The reaction of 2-aminobenzamide with phthalic acid anhydride In dioxane produced a bicyclic product 2,8-dioxoisoindole(1.2.a) quinazoline (1) in addition to hydrolysis product 2(2-Carboxyphenyl)-1.2-2H-quinazoline-4-one(Ⅱ). The yields were 64% and 30% respectively. On the other hand, the same reaction in DMF afforded compound(Ⅰ) and 2(2N-dimethyl carbamyl phenyl)-1.4-2H-quinazoline-4-one(Ⅲ) in 30% and 60% yield respectively. The compound Ⅲ was also obtained by the reaction of compoundⅡ with dimethylamine. However the reaction of 2-aminobenzamide with neat succinic acid anhydride gave only bicycle product 2.8-oxopyrrolidine (2.1.a)-1.4-2H-quinazoline (Ⅳ) in 93%.

2-Aminobenzamide로부터 Quinazoline 4-one계 유도체의 합성(III) Acid anhydride와의 반응

서명은(Myung Eun Suh) 大韓藥學會 1990 약학회지 Vol.34 No.2

The reaction of 2-aminobenzamide with phthalic acid anhydride in dioxane produced a bicyclic product 2,8-dioxoisoindole(1,2,a) quinazoline (I) in addition to hydrolysis product 2(2-Carboxyphenyl)-1,2-2H-quinazoline-4-one (II). The yields were 64% and 30% respectively. On the other hand, the same reaction in DMF afforded compound(I)and 2(2N-dimethyl carbamyl phenyl)-1,4-2H-quinazoline-4-one(III) in 30% and 60% yield respectively. The compound III was also obtained by the reaction of compound II with dimethylamine. However the reaction of 2-aminobenzamide with neat succinic acid anhydride give only bicyclic product 2,8-oxopyrrolidine (2,1,a)-1,4-2H-quinazoline (IV) in 93%.

Effect of Persimmon Leaf Extract on Phthalic Anhydride-induced Allergic Response in Mice

Mok, Ji-Ye,Jeon, In-Hwa,Cho, Jung-Keun,Park, Ji-Min,Kim, Hyeon-Soo,Kang, Hyun-Ju,Kim, Hyung-Soon,Jang, Seon-Il The Korean Society of Food Science and Nutrition 2012 Preventive Nutrition and Food Science Vol.17 No.1

The purpose of this study was to investigate the anti-allergy activities of persimmon leaf extract (PLE) on a phthalic anhydride (PA)-induced allergic mouse model. A human leukemic mast cell line (HMC-1) was used to examine the inhibitory activity of PLE on the histamine release by human leukemic mast cells. PLE inhibited histamine release from HMC-1 cells in response to cross-linkage of high-affinity IgE receptor-${\alpha}$ ($Fc{\varepsilon}RI{\alpha}$). Additionally, a PA-induced allergic mouse model was used to investigate the effects of PLE in vivo. Mice were orally administrated with or without PLE of single dose (250 mg/kg/day) for 31 days. Oral intake of PLE significantly inhibited passive cutaneous reactions. Oral administration of PLE to PA-induced allergic mice also led to a striking suppression of the development of contact dermatitis, ear swelling and lymph node weight. In addition, PA-specific IL-4 production of draining lymph node cells was markedly diminished by PLE oral administration, but not IFN-${\gamma}$. Furthermore, PLE treatment suppressed PA-induced thymus and activation-regulated chemokine (CCL17) and cutaneous T cell-attracting chemokine (CCL27) expressions in ear tissues. Based on these results, we suggest that PLE may have therapeutic potential as an effective material for management of irritant contact dermatitis or related inflammatory diseases.

Anti-inflammatory effect of methanol extract of Capparis ecuadorica in phthalic anhydride-induced atopic dermatitis of IL-4/Luc/CNS-1 transgenic mice

Su Jin Lee,Bo Ram Song,Ji Eun Kim,Hyeon Jun Choi,Su Ji Bae,Yun Ju Choi,Jeong Eun Gong,Dae Youn Hwang 한국실험동물학회 2022 한국실험동물학회 학술발표대회 논문집 Vol.2022 No.1

Effect of Persimmon Leaf Extract on Phthalic Anhydride-induced Allergic Response in Mice

Ji Ye Mok,In Hwa Jeon,Jung-Keun Cho,Ji Min Park,Hyeon Soo Kim,Hyun Ju Kang,Hyung Soon Kim,Seon Il Jang 한국식품영양과학회 2012 Preventive Nutrition and Food Science Vol.17 No.1

The purpose of this study was to investigate the anti-allergy activities of persimmon leaf extract (PLE) on a phthalic anhydride (PA)-induced allergic mouse model. A human leukemic mast cell line (HMC-1) was used to examine the inhibitory activity of PLE on the histamine release by human leukemic mast cells. PLE inhibited histamine release from HMC-1 cells in response to cross-linkage of high-affinity IgE receptor-α (FcεRIα). Additionally, a PA-induced allergic mouse model was used to investigate the effects of PLE in vivo. Mice were orally administrated with or without PLE of single dose (250 ㎎/㎏/day) for 31 days. Oral intake of PLE significantly inhibited passive cutaneous reactions. Oral administration of PLE to PA-induced allergic mice also led to a striking suppression of the development of contact dermatitis, ear swelling and lymph node weight. In addition, PA-specific IL-4 production of draining lymph node cells was markedly diminished by PLE oral administration, but not IFN-γ. Furthermore, PLE treatment suppressed PA-induced thymus and activation-regulated chemokine (CCL17) and cutaneous T cell-attracting chemokine (CCL27) expressions in ear tissues. Based on these results, we suggest that PLE may have therapeutic potential as an effective material for management of irritant contact dermatitis or related inflammatory diseases.

Capparis ecuadorica extract as an anti-inflammatory agent in phthalic anhydride-induced atopic dermatitis of IL-4/Luc/CNS-1 transgenic mice

Su Jin Lee,Bo Ram Song,Ji Eun Kim,Hyeon Jun Choi,Su Ji Bae,Yun Ju Choi,Jeong Eun Gong,Dae Youn Hwang 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.1

고본(藁本)내 정유성분의 생리활성 탐색

김민회,김영길,이진하,홍거표,홍정기,공영준,이현용 한국산업미생물학회 2000 한국미생물·생명공학회지 Vol.28 No.2

고본에서 정유성분을 추출하여 표준물질과 함께 생리활성 실험을 하였으며, 전체적으로 고본에서 추출한 정유성분이 순수한 표준물질인 phthalic anhydride보다 생리활성능이 좋았다. 항암효과 및 돌연변이 유발 억제실험에서는 고본이 표준물질보다 약 60% 높은 억제 효과를 나타내었으며, 암세포 성장저해에서 고본의 정유성분은 1.0 ㎎/㎖의 농도에서 50%이상의 억제율을 보였으나, 표준물질은 40%이하의 낮은 억제율을 나타내었고, 정상세포에 대한 세포독성을 측정한 결과 고본의 정유와 표준물질이 1.0 ㎎/㎖의 농도에서 각각 34.4%, 26.4%의 낮은 세포독성을 나타내었다. 또한 항돌연변이원성 실험에 rec-assay와 CHO V79 cell에서 고본의 정유가 표준물질 보다 더 효과적이었다. 그리고 면역기능 효과 실험에서 고본의 정유성분은 표준물질보다 1.0 ㎎/㎖의 농도에서 1.21배의 증강을 보였다. 또한 고본 정유성분의 혈당강하, 혈압조절(ACE활성 저해), GST활성, 혈전용해능 등도 활성율이 뛰어났으며, 각각 최고의 농도에서(㎎/㎖) 59.2%, 80.1%, 301.2%, 42.9%의 우수한 생리 활성능을 보였고, 표준물질인 경우 57.7%, 65.3%, 234.7%, 28.6%로 높지만, 고본의 정유성분 보다 상대적으로 낮은 활성능을 보였다. 그리고 신경세포의 생육 및 신경돌기 생성 촉진 실험에서 신경돌기 길이는 무첨가 보다 약 50 ㎛ 촉진하였으며, 신경돌기를 지니는 세포수는 2배 더 증가하였고, 생세포수는 약 56.4% 증가함을 나타냈다. 따라서 고본의 정유성분의 생리 활성능과 신경세포에 대한 우수한 활성능은 식물기원의 신경 활성물질의 탐색이 유효할 것으로 사료되어 진다. The biological activities of the crude essential oils from Ligusticum tenuissimum and the control(phthalic anhydride) were compared. About 60% of the growth of MCF7, A549, and Hep3B cells were inhibited by adding 1.0 ㎎/㎖ of the crude essential oils and below 40% was observed by the control. Cytotoxicity on human normal lung cell(IMR90) was scored as 34.4% for the crude oil and 26.4% for control, respectively. It was found that the crude essential oils were more effective than the control in anti mutagenecity tested by both Rec-assay and CHO V79 cells. The growth of human T-cell(Jurkat) was enhanced up to 1.21 times by adding the crude essential oil compared with the control. 50% of α-glucosidase activity was inhibited by both the crude essential oil and the control. ACE activities were inhibited 80.1% and 65.3% by adding 1.0 ㎎/㎖ of the crude oil and the control, respectively. The higher enhancement of glutathione-S-transferase activity was observed in the crude oil than those in the control : 301% v.s 234% at 1.0 ㎎/㎖ of the treatment. Thrombolytic activity was measured as 42.9% and 28.6% for the crude oil and the standard, respectively. The effect of the oil on the nerve cells PC12, was observed as follows: the neurite of PC12 cells was lengthened up to 255 ㎛ longer than 205 ㎛ of control. The number of neurite-bearing cells were about two times higher than control. The survival ratio of the crude essential oil was also increased up to 56.4% which was about two fold higher than in control.

Anti-Inflammatory Effects of Bee Venom on Phthalic Anhydride-Induced Atopic Dermatitis

Oh, Myung Jin,Song, Ho-Sueb Korean AcupunctureMoxibustion Medicine Society 2020 대한침구의학회지 Vol.19 No.4

Background: Atopic dermatitis (AD) is a chronic inflammatory condition which can be studied using phthalic anhydride (PA) to induce AD. Anti-inflammatory properties of bee venom (BV) wereinvestigated to determine whether it may be a useful treatment for AD. Methods: AD was induced by applying to pical PA to 8-week-old HR-1 mice (N = 50), then treating with (0.1, 0.25, and 0.5 ㎍) or without topical BV. Body weight, ear thickness histology, enzyme-linked immune sorbent assay (serum IgE concentrations), Western blot analysis [inducible nitric oxide synthase, cyclooxygenase-2, IκB-α, phospho-IκB-α, c-Jun N-terminal kinase (JNK), phospho-JNK, p38, phospho-p38, extra cellular signal-regulated kinase (ERK), and phospho-ERK], and the pull down assay for immunoblotting (p50), were used to measure inflammatory mediators. Results: PA + BV (0.1, 0.25, and 0.5 ㎍) significantly decreased ear thickness without altering body weight. IgE concentrations decreased in the PA + BV (0.5 ㎍)-treated groups compared with PAtreatment. Tumor necrosis factor-α, interleukin-1β, inducible nitric oxide synthase, cyclooxygenase-2, phospho-IκB-α, phospho-JNK, p38, phospho-p38, and phospho-ERK, all decreased following treatment with PA + BV compared with the PA-treatment alone. p50 was upregulated in the PA + BV-treated groups compared with the PA-treated group. Furthermore, the number of mast cells decreased in the PA + BV-treated groups compared with the PA-treated group. Epidermal thickness was significantly lower in the PA + BV-treated group compared with PA treatment alone. Conclusion: BV maybe a useful anti-inflammatory treatment for AD.