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액상정액을 이용한 인공수정시 품종, 계절, 인공수정 횟수 및 정자농도가 번식성적에 미치는 영향
김인철,박창식,이규승,서길웅,한성욱 충남대학교 농업과학연구소 2001 농업과학연구 Vol.28 No.2
돼지에서 액상정액을 이용한 인공수정이 번식성적에 미치는 영향을 구명하기 위하여 축산기술연구소 종축개량부 (충남, 성환) 돼지 인공수정센터에서 사육중인 인공수정용 종모돈을 이용하여 1995년부터 2000년까지 액상정액을 이용한 인공수정시 종모돈의 품종, 인공수정횟수, 계절 및 정자농도가 번식성적에 미치는 영향을 조사하여 돼지 인공수정시 번식성적 향상과 실용화에 기여하고자 본 연구를 실시하였다. 액상정액 인공수정시 정액을 생산한 종모돈의 품종 (Landrace, Yorkshire, Duroc)간 번식성적은 유의차가 인정되지 않았다. 발정당 2회 또는 3회 인공수정 시에도 번식성적에 차이가 없었다. 인공수정을 실시한 계절별로 번식성적을 조사하였으나 계절간 차이점은 발견할 수 없었다. 액상정액 인공수정시 1회 주입정자의 최저농도를 구명코자 활력 70% 이상인 정자수를 기준으로 80 ml 병당 정자농도를 3.0, 2.5, 2.0 및 1.5×10^(9)로 조절하여 인공수정한 결과 수태율, 분만율, 총산자수 및 생존산자수에서 통계적인 유의차가 인정되지 않아 1회 주입정자농도는 운동성 정자 기준으로 1.5×10^(9)/80 ml 이상으로 조절하면 인공수정에 실용적으로 사용 할 수 있음을 보였다. This study was carried out to investigate the effects of liquid boar semen on reproductive performance in swine artificial insemination Many factors, which were breeds, time of insemination, breeding season, sperm per dose etc. have been tried to improve reproductive efficiency. Boars were raised at Swine Artificial Insemination Center in National Livestock Research Institute. Sunghwan, Chungnam, Korea. This experiment was carried out from 1995 to 2000. There were no differences in the fertility results compared with 3 breeds (Landrace, Yorkshire and Duroc), frequencies of artificial insemination (double and triple) per estrus cycle and different seasons by using liquid boar semen. There were no significant differences in conception rate, farrowing rate and litter size using 4 trials of 3.0, 2.5, 20 and 1.5×10^(9)/80㎖ in liquid boar semen with 70% of motile sperm cells. We confirmed that the sperm number per dose of 1.5×10^(9)/80㎖ could be used for commercial artificial insemination.
칡소 동결 정액 생산을 위한 스티로폼상자와 액체질소 이용 방법
김성우(Sung Woo Kim),고응규(Yeoung-Gyu Ko),이재영(Jae-Yeong Lee),김찬란(Chan-Lan Kim),황인설(In-Sul Hwang) 한국산학기술학회 2020 한국산학기술학회논문지 Vol.21 No.4
가축유전자원으로서 동결정액을 생산하는 가장 쉬운 방법은 스티로폼박스를 이용한 간이동결법으로 알려져 있다. 본 연구에서는 스티로폼 동결박스를 제작하여 가축의 동결정액 생산에 활용하는 방법을 검토하였으며 칡소 동결정액 생산을 최적화 할 수 있는 방법을 제시하고자 박스의 크기, 액체질소와 노출된 정액 스트로와 거리, 노출시간 및 생산량을 검토하였다. 2가지 동결박스를 비교하여 자료를 확보하였으며 내부 크기는 세로×가로×높이가 23.5×30.5×22.5cm와 25.5×46.5×26.5 cm로 측정되었다. 액체질소를 5cm 높이로 채우고 액체질소 위 2, 5 및 8cm 높이에서 동결하여 융해 후 생존성을 조사하였다. 칡소 정액을 동결할 경우, 액체질소와의 노출시간은 모두 10분이 적절하였으며 25.5×46.5×26.5 cm 크기의 상자가 높은 생존율을 보여주었다(60.4±5.3% 대 67.2±3.1%). 동결 상자의 최적화 공간은 정자 동결에 가장 중요한 요소로 판단되며 1회 동결 시 최대 생산 가능한 칡소 동결정액은 60개 이상으로 증가시킬 수 있었다. 이러한 정보를 활용하면, 축종에 따라 동결 정액 생산량 결정하고 목적에 맞는 용기를 활용하여 효율적인 동결정액 생산이 가능할 것으로 판단된다. A styrofoam box is used as a simple and easy freezing method to preserve animal semen as a livestock genetic source. This study optimized the methods of freezing chikso brindled cattle semen. To test the freezing box, the motility of spermatozoa was compared between two box sizes (length×width×heigh) with the dimensions of 23.5×30.5×22.5 cm and 25.5×46.5×26.5 cm. The motility of thawed sperm from brindled Korean bulls was used to confirm the efficiency of the freezing boxes. The box having a larger inner space with larger horizontal and height measurements supported better motility after thawing (60.4±5.3% vs 67.2±3.1%) with 10 min of exposure time in liquid nitrogen vapor. The optimized freezing space is estimated to be an essential element for successful freezing results and the larger box could be used for production of more than 60 frozen semen straws. These properties are also helpful to optimize the cryopreservation techniques that would control the quality and quantity of semen straws according to different animal species.
Yi, Y.J.,Li, Z.H.,Kim, E.S.,Song, E.S.,Kim, H.B.,Cong, P.Q.,Lee, J.M.,Park, Chang-Sik Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.2
This study was designed to analyze boar sperm to compare motility, acrosome morphology, viability and ATP by various preservation methods between Duroc boar A with cold shock resistance sperm and Duroc boar B with cold shock sensitivity sperm. Semen volume, sperm concentration, motility and normal acrosome between Duroc boar A and B did not show any differences within 2 h after collection. There were no differences in sperm motility and normal acrosome between boar A and B at 1 day of preservation at $17^{\circ}C$ and $4^{\circ}C$, respectively. However, sperm motility and normal acrosome from 2 day of preservation at $17^{\circ}C$ and $4^{\circ}C$, respectively, were higher for boar A than boar B. The frozen-thawed sperm motility and normal acrosome were higher for boar A than boar B. The sperm viability and ATP concentration according to storage period of liquid semen at $17^{\circ}C$ and $4^{\circ}C$ were higher for boar A than boar B. Also, the sperm viability and ATP concentration of frozen-thawed semen were higher for boar A than boar B. In conclusion, we found out that the original quality of boar semen with cold shock resistance sperm played an important role.
Park, Chang-Sik,Yi,Young-Joo,Li, Zhao-Hua,Kim, Eui-Sook,Song, Eun-Sook,Kim, Hong-Bin,Cong, Pei-Qing,Lee, Jang-Mi 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10
This study was designed to analyze boar sperm to compare motility, acrosome morphology, viability and ATP by various preservation methods between Duroc boar A with cold shock resistance sperm and Duroc boar B with cold shock sensitivity sperm. Semen volume, sperm concentration, motility and normal acrosome between Duroc boar A and B did not show any differences within 2 h after collection. There were no differences on sperm motility and normal acrosome between boar A and B at 1 day of preservation at 17℃ and 4℃, respectively. But sperm motility and normal acrosome from 2 day of preservation at 17℃ and 4℃, respectively, were higher on boar A than boar B. The frozen-thawed sperm motility and normal acrosome were higher on boar A than on boar B. The sperm viability and ATP concentration according to storage period of liquid semen at 17℃ and 4℃ were higher on boar A than on boar B. Also, the sperm viability and ATP concentration of frozen-thawed semen were higher on boar A than on boar B. In conclusion, we found out that the original quality of boar semen with cold shock resistance sperm played an important role.
Yi, Y.J.,Li, Z.H.,Kim, E.S.,Song, E.S.,Cong, P.Q.,Zhang, Y.H.,Lee, S.H.,Lee, J.W.,Park, Chang-Sik Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.8
This study was designed to develop a method of liquid storage of boar sperm at $4^{\circ}C$ by using the modified Beltsville F5 (BF5) diluent with bovine serum albumin (BSA) and N-acetyl-D-glucosamine. Boar sperm were stored in lactose-egg yolk and N-acetyl-D-glucosamine (LEN), BF5 and Golden-Pig liquid 4 (GPL4) diluents at $4^{\circ}C$ for 5 days and were examined for sperm viability, adenosine triphosphate (ATP) and in vitro fertilization (IVF). The percentage of sperm viability in GPL4 diluent was higher than in LEN and BF5 diluent from 1 to 5 days of storage at $4^{\circ}C$. The percentage of sperm viability steadily declined from 1 to 5 days of storage in the three different diluents. Sperm ATP in GPL4 diluent was higher than in LEN and BF5 diluents from 1 to 5 days of storage. Sperm ATP rapidly declined after 5 days of storage in the three different diluents. Porcine oocytes matured in vitro were inseminated with different sperm concentrations of liquid semen stored for 3 days in GPL4 diluent. The percentage of monospermic oocytes did not show any differences from 2.5 to $20{\times}10^5$ sperm/ml. However, the percentage of polyspermic oocytes in the sperm concentration of $2.5{\times}10^5$ sperm/ml was lower than in concentrations of 5, 10 and $20{\times}10^5$5 sperm/ml. The percentage of blastocysts from the cleaved oocytes at $2.5{\times}10^5/ml$ sperm concentration was significantly lower than at 5, 10 and $20{\times}10^5sperm/ml$ concentrations. In conclusion, GPL4 diluent can be stored at $4^{\circ}C$ for 5 days and showed higher sperm viability and sperm ATP concentration compared with LEN and BF5 diluents. Also, we found that GPL4 diluent can be used for IVF of porcine oocytes.
사수진,우제석,홍준기,김기현,김두완,김용민,박현식,김시주,정기화,조은석 한국수정란이식학회 2015 한국동물생명공학회지 Vol.30 No.3
The aim of this study is to examine current status of swine AI and boar stud in South Korea using survey and data analysis. This survey included 48 boar studs registered as ‘semen processing business’. The survey data were collected by direct visitation, FAX and/or telephone conversation for 7 months from June through December in 2013. 48 boar studs owned a total of 3,537 boars and the Duroc breed accounted for the highest rate (75.3%) of all boar breeds. In case of ownership, agricultural management corporations was the highest (50.0%) and followed by individual ownership (33.3%). Large-scale boar studs in terms of own over 151 boar were surveyed as 4.2% and most boar studs owned less than 100 boars (77.1%). The amount of liquid semen provided by 48 boar studs were 1,889,000 doses and each boar stud provided average of 39,000 does, which is represented for 90% consumption by sows in South Korea.