Comparison of Motility, Acrosome, Viability and ATP of Boar Sperm with or without Cold Shock Resistance in Liquid Semen at 17℃ and 4℃, and Frozen-thawed Semen

다국어 초록 (Multilingual Abstract)

This study was designed to analyze boar sperm to compare motility, acrosome morphology, viability and ATP by various preservation methods between Duroc boar A with cold shock resistance sperm and Duroc boar B with cold shock sensitivity sperm. Semen volume, sperm concentration, motility and normal acrosome between Duroc boar A and B did not show any differences within 2 h after collection. There were no differences on sperm motility and normal acrosome between boar A and B at 1 day of preservation at 17℃ and 4℃, respectively. But sperm motility and normal acrosome from 2 day of preservation at 17℃ and 4℃, respectively, were higher on boar A than boar B. The frozen-thawed sperm motility and normal acrosome were higher on boar A than on boar B. The sperm viability and ATP concentration according to storage period of liquid semen at 17℃ and 4℃ were higher on boar A than on boar B. Also, the sperm viability and ATP concentration of frozen-thawed semen were higher on boar A than on boar B. In conclusion, we found out that the original quality of boar semen with cold shock resistance sperm played an important role.