국가 기술개발 전략 실증분석을 통한 R&D 효율성 제고방안 연구

김은정(Eun Jeong Kim),전은진(Eun Jin Jun) 한국기술혁신학회 2012 기술혁신학회지 Vol.15 No.2

농림수산식품 산업은 기존의 생산 중심의 1차 산업을 탈피하여 미래 신성장동력 산업으로의 도약을 목표로 농업 R&D 패러다임의 전면적인 전환을 도모하고 있으며, 특히 농생명자원을 활용한 생명산업등의 육성을 주요 과제로 선정하고 범부처?청 차원에서 R&D 지원을 강화하고 있다. 본 연구에서는 생명산업의 핵심자원인 농생명자원의 확보?관리 및 활용 등 전 단계에 걸친 국가 R&D 정책, 기술개발 전략, 사업 추진현황, 성과 등에 대한 실증분석을 통해 문제점을 도출하고 실용화 조기 달성을 위한 개선방안을 제안하고자 한다. 궁극적으로 제안된 개선방안으로부터 농생명자원 R&D 분야의 정부/민간, 부처(청) 내 역할분담 및 협력방안을 제시함으로써, 정부 R&D 투자의 전략성?효율성을 강화하고 적극적인 민간기업의 참여를 통한 생명산업의 산업화 성공사례 조기 달성에 기여할 것으로 기대된다. Once a production focused on primary industry, Food, agriculture, forestry and fisheries industry is now trying to shed away and plans to totally transform the paradigm of agricultural R&D with a leaping goal towards being a new growth engine. Promoting the bioindustry through utilizing agricultural bioresources was selected as the primary task and also made all efforts to support by all government department and ministry. In this study, we are raised problems and planned the improvements by the empirical analysis of national R&D policy, investment strategy, the recent progress of R&D program, and the performance of R&D investment to achieve early commercialization in fields of agricultural bioresources utilizing. We also propose the R&D role sharing and cooperations of public/ private sector to commercialize the agricultural bioresources from the comprehensive empirical analysis results. It will eventually strengthen the national R&D investment strategy and efficiency and then will contribute towards a successful early commercialization performance in bioindustry through utilizing agricultural bioresources.

남한에서 기후변화에 따른 난아열대 목본식물, Myrica rubra와 Syzygium buxifolium의 잠재분포 변화 예측

임은영,원현규,원종서,김다나,조형진 응용생태공학회 2022 Ecology and resilient infrastructure Vol.9 No.4

Analyzing the impact of climate change on the Korean Peninsula on the forest ecosystem is important for the management of subtropical forest bioresources. In this study, we collected location data and bioclimatic variables of the warm-temperate woody plant species, Myrica rubra and Cyzygium buxifolium, and applied the MaxEnt model based on the collected data to estimate the potential distribution area. Precipitation and temperature seasonality in the warmest quarter were the main environmental factors that determined the distribution of M. rubra, and the main environmental factors for S. buxifolium were precipitation in the warmest quarter and precipitation in the wettest quarter. The results of the MaxEnt model by administrative district, the M. rubra showed an area increase rate of 4.6 - 17.7% in the SSP2-4.5 climate change scenario and 13.8 - 30.5% in the SSP5-8.5 climate change scenario. S. buxifolium showed area increase rates of 4.8 - 32.2% in the SSP2-4.5 climate change scenario and 12.9 - 48.6% in the SSP5-8.5 climate change scenario. This study is meaningful in establishing a database and identifying future potential distribution areas of warm and subtropical plants by applying climate change scenarios. 한반도의 기후변화가 산림생태계에 미치는 영향을 분석하는 것은 난아열대 산림생명자원 관리에 중요하다. 본 연구에서는난아열대 목본식물인 소귀나무와 Syzygium buxifolium의 위치자료와 생물기후변수를 수집하고, 수집된 자료를 바탕으로 MaxEnt 모형에 적용하여 잠재분포 영역을 추정하였다. 소귀나무의 분포를 결정하는 주요 환경인자는 가장 따뜻한 분기의 강수와 온도계절성이고, Syzygium buxifolium의 주요 환경인자는 가장 따듯한 분기의 강수와 가장 습한 분기의 강수로 나타났다. MaxEnt 모형의 행정구역별 결과, 소귀나무는 SSP2-4.5 기후변화 시나리오에서 4.6 - 17.7%의 면적 증가율을 보였고, SSP5-8.5 기후변화시나리오에서 13.8 - 30.5%의 면적 증가율을 보였다. Syzygium buxifolium는 SSP2-4.5 기후변화 시나리오에서 4.8 - 32.2%, SSP5-8.5 기후변화 시나리오에서 12.9 - 48.6%의 면적 증가율을 보였다. 본 연구는 기후변화 시나리오를 적용하여 난대아열대식물의 미래 잠재분포 영역을 확인하고 데이터베이스를 구축하는데 의의가 있다.

Establishment and application of information resource of mutant mice in RIKEN BioResource Research Center

Masuya Hiroshi,Usuda Daiki,Nakata Hatsumi,Yuhara Naomi,Kurihara Keiko,Namiki Yuri,Iwase Shigeru,Takada Toyoyuki,Tanaka Nobuhiko,Suzuki Kenta,Yamagata Yuki,Kobayashi Norio,Yoshiki Atsushi,Kushida Tatsu 한국실험동물학회 2021 Laboratory Animal Research Vol.37 No.1

Online databases are crucial infrastructures to facilitate the wide effective and efficient use of mouse mutant resources in life sciences. The number and types of mouse resources have been rapidly growing due to the development of genetic modification technology with associated information of genomic sequence and phenotypes. Therefore, data integration technologies to improve the findability, accessibility, interoperability, and reusability of mouse strain data becomes essential for mouse strain repositories. In 2020, the RIKEN BioResource Research Center released an integrated database of bioresources including, experimental mouse strains, Arabidopsis thaliana as a laboratory plant, cell lines, microorganisms, and genetic materials using Resource Description Framework-related technologies. The integrated database shows multiple advanced features for the dissemination of bioresource information. The current version of our online catalog of mouse strains which functions as a part of the integrated database of bioresources is available from search bars on the page of the Center ( https://brc.riken.jp ) and the Experimental Animal Division ( https://mus.brc.riken.jp/ ) websites. The BioResource Research Center also released a genomic variation database of mouse strains established in Japan and Western Europe, MoG + ( https://molossinus.brc.riken.jp/mogplus/ ), and a database for phenotype-phenotype associations across the mouse phenome using data from the International Mouse Phenotyping Platform. In this review, we describe features of current version of databases related to mouse strain resources in RIKEN BioResource Research Center and discuss future views.

Dissemination of Advanced Mouse Resources and Technologies at RIKEN BioResource Center

Yoshiki, Atsushi Korean Society for Bioinformatics 2010 Interdisciplinary Bio Central (IBC) Vol.2 No.4

RIKEN BioResource Center (BRC) has collected, preserved, conducted quality control of, and distributed mouse resources since 2002 as the core facility of the National BioResource Project by the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. Our mouse resources include over 5,000 strains such as humanized disease models, fluorescent reporters, and knockout mice. We have developed novel mouse strains such as tissue-specific Cre-drivers and optogenetic strains that are in high demand by the research community. We have removed all our specified pathogens from the deposited mice and used our quality control tests to examine their genetic modifications and backgrounds. RIKEN BRC is a founding member of the Federation of International Mouse Resources and the Asian Mouse Mutagenesis and Resource Association, and provides mouse resources to the one-stop International Mouse Strain Resource database. RIKEN BRC also participates in the International Gene Trap Consortium, having registered 713 gene-trap clones and their sequences in a public library, and is an advisory member of the CREATE (Coordination of resources for conditional expression of mutated mouse alleles) consortium which represents major European and international mouse database holders for the integration and dissemination of Cre-driver strains. RIKEN BRC provides training courses in the use of advanced technologies for the quality control and cryopreservation of mouse strains to promote the effective use of mouse resources worldwide.

해양생물자원의 골이식재 적용

장유리아,김보선,임원봉 한국키틴키토산학회 2023 한국키틴키토산학회지 Vol.28 No.2

In this study, we tried to examine the application of marine bioresource for bone graft materials. Bone is the skeletal structure that plays a physiologically pivotal role in the body. Various types of bone graft materials are being used to reconstruct the bone defect during dental and orthopedic surgery, and more optimized materials are being explored continuously. Bone graft materials derived from bioceramics are highly utilized in tissue engineering for bone regeneration, and generally provide hierarchical porosity, bioactivity and osteoconductive properties, and mechanical structures capable of 3D cell culture. These bone graft materials are generally based on porous calcium phosphate particles, which must maintain porosity once at the site of bone loss. However, despite routine use in traumatology and dental applications, biotransplantation requirements such as osteoinductivity and control of dissolution rates have not yet been met. Accordingly, bioceramic studies of marine-derived products are highly likely to become a new source of bone graft considering various marine materials that have not yet been fully identified. In this study, we tried to explore the application potential of major marine bioresources for bone tissue regeneration as bioceramics. In the present study, commercialized products and candidates in the preclinical research stage were reviewed, and major clinical requirements for the application of various available marine resources and the advantages and limitations of currently available bioresource were presented

국내 바이오산업의 산림생명자원 소재 이용 현황 분석

민경택 ( Kyungtaek Min ) 한국산림경제학회 2022 산림경제연구 Vol.29 No.1

이 연구의 목적은 바이오산업의 산림생명자원 이용 현황과 장애요인을 조사하고 해당 산업의 발전방안을 모색하는 데 있다. 이를 위해 바이오기업체 150개를 대상으로 설문조사를 실시하였다. 이 가운데 산림생명자원 소재를 이용하는 업체는 전체에서 30%로 나타났다. 이 업체의 매출액에서 산림생명자원 소재 구입의 지출 비중은 17%로 조사되었다. 이를 바탕으로 바이오산업에서 산림생명자원 소재 시장의 규모는 약 5,108억 원으로 추정하였다. 산림생명자원 소재를 국내에서 확보하는 것은 천연소재 73.0%, 가공소재 67.3%로 나타났다. 바이오사업체가 산림생명자원 소재를 확보하는 데 겪는 애로사항은 가격과 품질로 나타났다. 또, 바이오사업체가 관심갖는 산림생명자원 소재는 바이오의약산업은 산약초류와 약용수목류, 바이오식품산업은 산약초류와 임산버섯, 바이오에너지와 바이오환경산업은 목재자원인 것으로 나타났다. The purposes of the research are to investigate the current status and hurdles of using forest bioresources in bioindustry, and to make policy suggestions to foster both bioindustry and forestry. To achieve the research purposes, the survey was conducted to 150 biobusiness companies. The 30 percents of these companies use forest bioresources as raw material. And the expenditure to procure the forest bioresources is 17 percents of the products sales. On the basis of the result, the market size of forest bioresource in bioindustry is estimated about 510.8 billion KRW. On the procuring method, the share from domestic is 73.0 percents in natural material, 67.3 percents in processed material. The hurdles to procure the forest bioresources from domestic are price and quality. The promising forest bioresources are medicinal plants in biopharmaceutical industry, medicinal herbs and mushirooms in biofood industry, wood resources in bioenergy and bioenvironmental industry.

Cereal Resources in National BioResource Project of Japan

Sato, Kazuhiro,Endo, Takashi R.,Kurata, Nori Korean Society for Bioinformatics 2010 Interdisciplinary Bio Central (IBC) Vol.2 No.4

The National BioResource Project of Japan is a governmental project to promote domestic/international research activities using biological resources. The project has 27 biological resources including three cereal resources. The core center and sub-center which historically collected the cereal resources were selected for each cereal program. These resources are categorized into several different types in the project; germplasm, genetic stocks, genome resources and database information. Contents of rice resources are wild species, local varieties in East and Southwest Asia & wild relatives, MNU-induced chemical mutant lines, marker tester lines, chromosome substitution lines and other experimental lines. Contents of wheat resources are wild strains, cultivated strains, experimental lines, rye wild and cultivated strains; EST clones and full-length cDNA clones. Contents of barley resources are cultivar and experimental lines, core collection, EST/cDNA clones, BAC clones, their filters and superpool DNA. Each resource is accessible from the online database to see the contents and information about the resources. Links to the genome information and genomic tools are also important function of each database. The major contents and some examples are presented here.

Systematic Development of Tomato BioResources in Japan

Ariizumi, Tohru,Aoki, Koh,Ezura, Hiroshi Korean Society for Bioinformatics 2011 Interdisciplinary Bio Central (IBC) Vol.3 No.1

Recently, with the progress of genome sequencing, materials and information for research on tomato (Solanum lycopersicum) have been systematically organized. Tomato genomics tools including mutant collections, genome sequence information, full-length cDNA and metabolomic datasets have become available to the research community. In Japan, the National BioResource Project Tomato (NBRP Tomato) was launched in 2007, with aims to collect, propagate, maintain and distribute tomato bioresources to promote functional genomics studies in tomato. To this end, the dwarf variety Micro-Tom was chosen as a core genetic background, due to its many advantages as a model organism. In this project, a total of 12,000 mutagenized lines, consisting of 6000 EMS-mutagenized and 6000 gamma-ray irradiated M2 seeds, were produced, and the M3 offspring seeds derived from 2236 EMS-mutagenized M2 lines and 2700 gamma-ray irradiated M2 lines have been produced. Micro-Tom mutagenized lines in the M3 generation and monogenic Micro-Tom mutants are provided from NBRP tomato. Moreover, tomato cultivated varieties and its wild relatives, both of these are widely used for experimental study, are available. In addition to these bioresources, NBRP Tomato also provides 13,227 clones of full-length cDNA which represent individual transcripts non-redundantly. In this paper, we report the current status of NBRP Tomato and its future prospects.

Analysis of Human Tissue Management Models for Medical Research: Preparation for Implementation of the 2012 Revision of the Bioethics and Safety Act of Korea

류영준,김한겸,장세진,구영모 대한의학회 2013 Journal of Korean medical science Vol.28 No.6

Efficient management of human tissue samples is a critical issue; the supply of samples is unable to satisfy the current demands for research. Lack of informed consent is also an ethical problem. One of the goals of the 2012 revision of Korea’s Bioethics and Safety Act was to implement regulations that govern the management of human tissue samples. To remain competitive, medical institutions must prepare for these future changes. In this report, we review two tissue management models that are currently in use; model 1 is the most common system utilized by hospitals in Korea and model 2 is implemented by some of the larger institutions. We also propose three alternative models that offer advantages over the systems currently in use. Model 3 is a multi-bank model that protects the independence of physicians and pathologists. Model 4 utilizes a comprehensive single bioresource bank; although in this case, the pathologists gain control of the samples,which may make it difficult to implement. Model 5, which employs a bioresource utilization steering committee (BUSC), is viable to implement and still maintains the advantages of Model 4. To comply with the upcoming law, we suggest that physicians and pathologists in an institution should collaborate to choose one of the improved models of tissue management system that best fits for their situation.

Depth profiles of methane oxidation potentials and methanotrophic community in a lab-scale biocover

Lee, E.H.,Moon, K.E.,Kim, T.G.,Cho, K.S. Elsevier Science Publishers 2014 Journal of biotechnology Vol.184 No.-

The depth profiles of the CH<SUB>4</SUB> oxidation potentials and the methanotrophic community were characterized in a lab-scale soil mixture biocover. The soil mixture samples were collected from the top (0-10cm), middle (10-40cm), and bottom (40-50cm) layers of the biocover where most of methane was oxidized at the top layer due to consumption of O<SUB>2</SUB>. Batch tests using serum bottles showed that the middle and bottom samples displayed CH<SUB>4</SUB> oxidation activity under aerobic conditions, and their CH<SUB>4</SUB> oxidation rates were 85 and 71% of the rate of top sample (8.40μmolgdry sample<SUP>-1</SUP>h<SUP>-1</SUP>), respectively. The numbers of methanotrophs in the middle and bottom were not significantly different from those in the top sample. There was no statistical difference in the community stability indices (diversity and evenness) among the methanotrophic communities of the three layer samples, even though the community structures were distinguished from each other. Based on microarray analysis, type I and type II methanotrophs were equally present in the top sample, while type I was more dominant than type II in the middle and bottom samples. We suggested that the qualitative difference in the community structures was probably caused by the difference in the depth profiles of the CH<SUB>4</SUB> and O<SUB>2</SUB> concentrations. The results for the CH<SUB>4</SUB> oxidation potential, methanotrophic biomass, and community stability indices in the middle and bottom layer samples indicated that the deeper layer in the methanotrophic biocover serves as a bioresource reservoir for sustainable CH<SUB>4</SUB> mitigation.