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RISS 인기검색어
- 검색키워드
- 주제어 : Zoysia japonica Steud (검색결과 5 건)
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송인자(In-Ja Song),선현진(Hyeon-Jin Sun),정옥철(Ok-Cheol Jeong),양대화(Dae-Hwa Yang),진일두(Il-Doo Jin),강홍규(Hong-Gyu Kang),고석민(Suk-Min Ko),권용익(Yong-Ik Kwon),배태웅(Tae-Woong Bae),송필순(Pill-Soon Song),이효연(Hyo-Yeon Lee) 한국육종학회 2017 한국육종학회지 Vol.49 No.1
This study was carried out to develop new zoysiagrass (Zoysia japonica Steud.) cultivar ‘Halla Green 2’ (Grant number: No. 118). To develop a zoysiagrass cultivar with dwarfism by using the mutation breeding method, the wild type control Gosan plants were irradiated using a 30 Gy gamma ray source in 2010. Dwarf mutants were selected from the mutated grasses in successive generations. Dwarf mutant lines were identified and a new zoysiagrass variety Halla Green 2 was developed. The plant height of Halla Green 2 was 3.4 and 1.8 times lower than that of Gosan and Zenith, respectively. This cultivar has dwarf characteristics such as shorter sheath, shorter leaf blade, shorter flag leaf, and shorter third internode of stolon compared to those of Gosan and Zenith. Additionally, the sheaths and leaf blades color of Gosan, Zenith and Halla Green 2 were all light green, whereas their stolons were purple, yellow-green and yellow green, respectively. Trichomes(hairs) were visible on both adaxial and abaxial surfaces of the Gosan leaves, whereas only on the adaxial side of the Zenith and Halla Green 2 leaves. The Halla Green 2 grass showed distinguishable morphological traits compared to those of wild type Gosan and Zenith.
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Production of Herbicide-tolerant Zoysiagrass by Agrobacterium-mediated Transformation
Koichi Toyama,배창휴,강정구,임용표,Taiji Adachi,Key-Zung Rui,송필순,이효연 한국분자세포생물학회 2003 Molecules and cells Vol.16 No.1
Herbicide-resistant zoysiagrass (Zoysia japonica Steud.) has been developed by Agrobacterium-mediated transformation. A callus-type transformation system was established by optimizing several factors that affect the rate of transformation, including co-cultivation period and concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D), CaCl2 and acetosyringone. Maximal GUS expression was observed when a Type 3 callus was cocultivated on 2,4-D-free co-cultivation medium for 9 d. In addition, removal of calcium and addition of 50−100 mg/L acetosyringone during co-cultivation enhanced GUS expression. When this optimized protocol was applied to the transformation of the bialaphos resistance gene (bar), four plants per 700 mg of infected calluses survived on the selective medium. DNA gel-blot analysis showed that two copies of the transgene had been integrated. After application of 2 g/L bialaphos for a week the transgenic plants survived herbicide spraying, while untransformed zoysiagrasses and invading weeds died. The herbicide-tolerant zoysiagrass will permit more efficient weed control in this widely cultivated turf grass.
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한국잔디에 발생하는 봄마름병(Rhizoctonia cerealis AG-D)의 가을철 발생보고
이정한,김정호,전창욱,심규열,곽연식 한국잔디학회 2019 Weed & Turfgrass Science Vol.8 No.4
In October 2018, symptoms of the spring dead spot (Rhizoctonia cerealis AG-D) disease in Kwang-Ju Country Club at Gokseong, Jeonnam province, South Korea were observed on zoysiagrass (Zoysia japonica Steud.). Spring dead spot symptom commonly appears in spring, however our research showed that the spring dead spot symptom on zoysiagrass could be shown in autumn. The initial disease symptoms were observed on reddish brown leaves and a decaying, irregular-shape patch of the leaf (30-50 cm), developed by the end of October, as the disease progressive. Aerial hyphae, like spider web on the leaves, were observed in the early morning. The causal agent was isolated and identified as Rhizoctonia cerealis AG-D by morphological and molecular characteristics. Optimal temperature and pH for mycelial growth of Rhizoctonia cerealis AG-D were 20℃ and pH 6.0, respectively. Bud formation from the infected tissue in fall season was considerably reduced compared to healthy lawn in spring. The spring dead spot infection site was a leaf auricle while the big patch was stemmed around the crown tissue. Thus sheath infected large patch was plucked easily than lawn spring dead spot pathogen. This is the first report of an outbreak of the spring dead spot disease on zoysiagrass in the fall season. 2018년 10월 중순 전남 곡성에 소재한 광주골프장에서 봄마름병 병징이 한국잔디에 발생하였다. 일반적으로 봄마름병의 감염시기는 늦은 가을이며 병징은 봄에 나타나지만 본 연구에서 조사한 바에 따르면 가을에 병징이 나타나는 것으로 조사되었다. 초기 병징은 잔디의 잎이 붉은 갈색으로 황화되기 시작하며 엽이와 줄기가 썩고 10월 말경에는30-50 cm의 불규칙한 패취 형태로 병반이 나타났다. 이른 아침 거미줄 모양의 기중균사가 형성되었다. 병원균의 형태학적 특징과 rDNA의 ITS 염기서열을 분석한 결과 Rhizoctonia cerealis AG-D로 동정되었다. 병원균의 최적 생장온도와pH조건은 20℃에서 pH 6으로 각각 나타났다. 가을에 감염된 잔디의 맹아 출현은 건전 잔디에 비하여 현저히 떨어지는것으로 나타났다. 봄마름병의 감염부위는 엽이이고 반면에 라이족토니아마름병은 관부 주위이기 때문에 봄마름병에 감염된 잔디는 라이족토니아마름병에 감염된 잔디보다 잘 뽑히지 않는 특징이 있다. 가을철 발생하는 한국잔디의 봄마름병을 처음으로 보고하고자 한다.
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들잔디 재배 시 적정 인산비료 시비량 구명을 위한 생육 및 토양 평가
배은지,정재경,윤준혁,진언주,전권석,김영선 한국잔디학회 2022 Weed & Turfgrass Science Vol.11 No.4
잔디 재배지의 경우 연작과 과도한 시비로 인한 토양 산성화로 인해 토양 내 인산 불용태화 될 우려가 있어 적정한 시비 기준이 필요한 실정이다. 본 연구는 양질의 들잔디 재배를 위한 적정 인산비료 시비량을 구명하기 위하여 인산비료농도별 처리에 따른 생육과 토양 화학성의 변화를 알아보고자 와그너 포트시험과 잔디 재배지 포장시험을 수행하였다. 와그너 포트시험에서는 지상부와 포복경 건물중이 인산 시비량이 증가함에 따라 증가하였고, 인산비료 시비량 18와 24 kg N 10a-1 시비량간의 유의한 차이를 보이지 않았다. 들잔디 재배지에서는 인산비료 시비량이 증가할수록 초장, 생체중과 건물중이 증가하였고, 인산비료 12와 18 kg 10a-1 시비량은 24 kg 10a-1 시비량과 비슷한 생육결과를 나타내었다. 지상부 개체수와 포복경 길이는 12, 18과 24 kg 10a-1 시비량간 유의한 차이가 없었다. 식물체내 인산 함량은 인산비료 24 kg 10a-1 시비량이 가장 높게 나타났지만, 칼륨, 마그네슘과 칼슘 함량은 인산비료 12, 18과 24 kg 10a-1 시비량과 비슷하였다. 그러나 토양을 조사한 결과 인산비료 24 kg 10a-1 시비량에서 전질소, 치환성 Ca2+과 Mg2+과 같은 양분이 감소하는 결과를 보여 과도한 인산비료 시비는 토양의 화학성이 악화되는 경향을 보였다. 잔디의 생육과 토양환경을 고려한 결과 들잔디 재배 시 적정 인산비료 시비량은 12 kg 10a-1로 판단되었다. Optimized application of phosphate fertilizer is needed for appropriate growth of zoysiagrass in the soil conditions such as its insolubility and less availability because of soil acidification by repeated cultivation and excessive fertilization. This study was conducted to find out the optimum phosphate fertilization for Zoysia japonica with good quality depending on the growth and chemical changes of the soil in pot and field experiments treated with different levels of phosphate fertilizer. In the pot experiment, the dry weight of shoots and stolons increased as phosphate fertilizer levels increased, and showed no significant difference between 18 and 24 kg N 10a-1. In the field experiment, plant height, fresh and dry weight of shoots, roots, and stolons increased with increasing phosphate fertilizer application, and 12 and 18 kg 10a-1 treated with phosphate fertilizer showed similar growth to 24 kg 10a-1. There was no significant difference in the number of shoots and total of stolons length between phosphate fertilizers 12, 18, and 24 kg 10a-1. The phosphoric acid content in the inorganic nutrient of the zoysiagrass was highest at phosphoric acid 24 kg 10a-1, but potassium, magnesium, and calcium contents were similar at phosphate fertilizer 12, 18, and 24 kg 10a-1. However, as a result of soil investigation, nutrients such as total nitrogen, exchangeable Ca2+ and Mg2+ decreased in phosphate fertilizer 24 kg 10a-1, and excessive phosphate fertilization displayed a tendency to aggravate the soil chemical properties. As a result of considering the growth of the zoysiagrass and the soil environment, the optimum amount of phosphate fertilizer was determined to be 12 kg 10a-1 in Z. japonica cultivation.
ScienceON
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