Genetic identification of Sinomenium acutum based on chloroplast gene ndhF sequences

( Jin Ah Ryuk ),( Hye Won Lee ),( Byoung Seob Ko ) 대한본초학회 2013 大韓本草學會誌 Vol.28 No.5

Objectives : This study was conducted to identify the original Sinomini Caulis et Rhizoma plant among Stephania tetrandra, Cocculus trilobus, and Aristolochiae fangchi to develop the genetic marker for Sinomini Caulis et Rhizoma. Methods : Sinomenium acutum was identified by the classification and identification committee of the National Center for Standardization of Herbal Medicines. The chloroplast ndhF gene was amplified. We performed sequences alignment analysis of Sinomenium acutum, Stephania tetrandra, C. trilobus, and A. fangchi using BioEdit program. The SFR markers designed were consisted of SF01, SR04, and SR05 primers. Results : Many variations of Sinomeni Caulis et Rhizoma are currently commercialized as herbal medicine. We compared the base sequences of the ndhF intergenic space of chloroplast DNA with Sinomenium acutum, Stephania tetrandra, C. trilobus, and A. fangchi. According to the results, it showed that the nucleotide variations were seen in 30 genes of four species. Phylogenetic analysis revealed that 4 species were classified into five groups based on an inter-group divergence in nucleotide sequence of 9%. We developed SFR marker nucleotides enough to authenticate respective species and confirmed its application on the band size at 419 base pair. These sequence differences at corresponding positions were available genetic markers to identity the Sinomeni Caulis et Rhizoma. Conclusions : Base on these results, the ndhF region was effective in distinguishing Sinomini Caulis et Rhizoma The SFR genetic marker was useful for identifying Sinomini Caulis et Rhizoma with other species.

Sinomenine, an Alkaloid Derived from Sinomenium acutum Potentiates Pentobarbital-Induced Sleep Behaviors and Non-Rapid Eye Movement (NREM) Sleep in Rodents

( Jae Hyeon Yoo ),( Tae-woo Ha ),( Jin Tae Hong ),( Ki-wan Oh ) 한국응용약물학회 2017 Biomolecules & Therapeutics(구 응용약물학회지) Vol.25 No.6

Sinomenium acutum has been long used in the preparations of traditional medicine in Japan, China and Korea for the treatment of various disorders including rheumatism, fever, pulmonary diseases and mood disorders. Recently, it was reported that Sinomenium acutum, has sedative and anxiolytic effects mediated by GABA-ergic systems. These experiments were performed to investigate whether sinomenine (SIN), an alkaloid derived from Sinomenium acutum enhances pentobarbital-induced sleep via γ-aminobutyric acid (GABA)-ergic systems, and modulates sleep architecture in mice. Oral administration of SIN (40 mg/kg) markedly reduced spontaneous locomotor activity, similar to diazepam (a benzodiazepine agonist) in mice. SIN shortened sleep latency, and increased total sleep time in a dose-dependent manner when co-administrated with pentobarbital (42 mg/kg, i.p.). SIN also increased the number of sleeping mice and total sleep time by concomitant administration with the sub-hypnotic dosage of pentobarbital (28 mg/kg, i.p.). SIN reduced the number of sleep-wake cycles, and increased total sleep time and non-rapid eye movement (NREM) sleep. In addition, SIN also increased chloride influx in the primary cultured hypothalamic neuronal cells. Furthermore, protein overexpression of glutamic acid decarboxylase (GAD_65/67) and GABA_A receptor subunits by western blot were found, being activated by SIN. In conclusion, SIN augments pentobarbital-induced sleeping behaviors through GABA_A-ergic systems, and increased NREM sleep. It could be a candidate for the treatment of insomnia.

Genetic identification of Sinomenium acutum based on chloroplast gene ndhF sequences

Ryuk, Jin Ah,Lee, Hye Won,Ko, Byoung Seob The Korea Association of Herbology 2013 大韓本草學會誌 Vol.28 No.5

Objectives : This study was conducted to identify the original Sinomini Caulis et Rhizoma plant among Stephania tetrandra, Cocculus trilobus, and Aristolochiae fangchi to develop the genetic marker for Sinomini Caulis et Rhizoma. Methods : Sinomenium acutum was identified by the classification and identification committee of the National Center for Standardization of Herbal Medicines. The chloroplast ndhF gene was amplified. We performed sequences alignment analysis of Sinomenium acutum, Stephania tetrandra, C. trilobus, and A. fangchi using BioEdit program. The SFR markers designed were consisted of SF01, SR04, and SR05 primers. Results : Many variations of Sinomeni Caulis et Rhizoma are currently commercialized as herbal medicine. We compared the base sequences of the ndhF intergenic space of chloroplast DNA with Sinomenium acutum, Stephania tetrandra, C. trilobus, and A. fangchi. According to the results, it showed that the nucleotide variations were seen in 30 genes of four species. Phylogenetic analysis revealed that 4 species were classified into five groups based on an inter-group divergence in nucleotide sequence of 9%. We developed SFR marker nucleotides enough to authenticate respective species and confirmed its application on the band size at 419 base pair. These sequence differences at corresponding positions were available genetic markers to identity the Sinomeni Caulis et Rhizoma. Conclusions : Base on these results, the ndhF region was effective in distinguishing Sinomini Caulis et Rhizoma The SFR genetic marker was useful for identifying Sinomini Caulis et Rhizoma with other species.

Genetic identification of Sinomenium acutum based on chloroplast gene ndhF sequences

육진아,이혜원,고병섭 대한본초학회 2013 大韓本草學會誌 Vol.28 No.5

Objectives : This study was conducted to identify the original Sinomini Caulis et Rhizoma plant among Stephania tetrandra, Cocculus trilobus, and Aristolochiae fangchi to develop the genetic marker for Sinomini Caulis et Rhizoma. Methods : Sinomenium acutum was identified by the classification and identification committee of the National Center for Standardization of Herbal Medicines. The chloroplast ndhF gene was amplified. We performed sequences alignment analysis of Sinomenium acutum, Stephania tetrandra, C. trilobus, and A. fangchi using BioEdit program. The SFR markers designed were consisted of SF01, SR04, and SR05 primers. Results : Many variations of Sinomeni Caulis et Rhizoma are currently commercialized as herbal medicine. We compared the base sequences of the ndhF intergenic space of chloroplast DNA with Sinomenium acutum, Stephania tetrandra, C. trilobus, and A. fangchi. According to the results, it showed that the nucleotide variations were seen in 30 genes of four species. Phylogenetic analysis revealed that 4 species were classified into five groups based on an inter-group divergence in nucleotide sequence of 9%. We developed SFR marker nucleotides enough to authenticate respective species and confirmed its application on the band size at 419 base pair. These sequence differences at corresponding positions were available genetic markers to identity the Sinomeni Caulis et Rhizoma. Conclusions : Base on these results, the ndhF region was effective in distinguishing Sinomini Caulis et Rhizoma The SFR genetic marker was useful for identifying Sinomini Caulis et Rhizoma with other species.

Sinomenine, an Alkaloid Derived from Sinomenium acutum Potentiates Pentobarbital-Induced Sleep Behaviors and Non-Rapid Eye Movement (NREM) Sleep in Rodents

유재현,하태우,홍진태,오기완 한국응용약물학회 2017 Biomolecules & Therapeutics(구 응용약물학회지) Vol.25 No.6

Sinomenium acutum has been long used in the preparations of traditional medicine in Japan, China and Korea for the treatment of various disorders including rheumatism, fever, pulmonary diseases and mood disorders. Recently, it was reported that Sinomenium acutum, has sedative and anxiolytic effects mediated by GABA-ergic systems. These experiments were performed to investigate whether sinomenine (SIN), an alkaloid derived from Sinomenium acutum enhances pentobarbital-induced sleep via γ-aminobutyric acid (GABA)-ergic systems, and modulates sleep architecture in mice. Oral administration of SIN (40 mg/kg) markedly reduced spontaneous locomotor activity, similar to diazepam (a benzodiazepine agonist) in mice. SIN shortened sleep latency, and increased total sleep time in a dose-dependent manner when co-administrated with pentobarbital (42 mg/kg, i.p.). SIN also increased the number of sleeping mice and total sleep time by concomitant administration with the sub-hypnotic dosage of pentobarbital (28 mg/kg, i.p.). SIN reduced the number of sleep-wake cycles, and increased total sleep time and non-rapid eye movement (NREM) sleep. In addition, SIN also increased chloride influx in the primary cultured hypothalamic neuronal cells. Furthermore, protein overexpression of glutamic acid decarboxylase (GAD65/67) and GABAA receptor subunits by western blot were found, being activated by SIN. In conclusion, SIN augments pentobarbital-induced sleeping behaviors through GABAA-ergic systems, and increased NREM sleep. It could be a candidate for the treatment of insomnia.

Sinomenine, an Alkaloid Derived from Sinomenium acutum Potentiates Pentobarbital-Induced Sleep Behaviors and Non-Rapid Eye Movement (NREM) Sleep in Rodents

Yoo, Jae Hyeon,Ha, Tae-Woo,Hong, Jin Tae,Oh, Ki-Wan The Korean Society of Applied Pharmacology 2017 Biomolecules & Therapeutics(구 응용약물학회지) Vol.25 No.6

Sinomenium acutum has been long used in the preparations of traditional medicine in Japan, China and Korea for the treatment of various disorders including rheumatism, fever, pulmonary diseases and mood disorders. Recently, it was reported that Sinomenium acutum, has sedative and anxiolytic effects mediated by GABA-ergic systems. These experiments were performed to investigate whether sinomenine (SIN), an alkaloid derived from Sinomenium acutum enhances pentobarbital-induced sleep via ${\gamma}$-aminobutyric acid (GABA)-ergic systems, and modulates sleep architecture in mice. Oral administration of SIN (40 mg/kg) markedly reduced spontaneous locomotor activity, similar to diazepam (a benzodiazepine agonist) in mice. SIN shortened sleep latency, and increased total sleep time in a dose-dependent manner when co-administrated with pentobarbital (42 mg/kg, i.p.). SIN also increased the number of sleeping mice and total sleep time by concomitant administration with the sub-hypnotic dosage of pentobarbital (28 mg/kg, i.p.). SIN reduced the number of sleep-wake cycles, and increased total sleep time and non-rapid eye movement (NREM) sleep. In addition, SIN also increased chloride influx in the primary cultured hypothalamic neuronal cells. Furthermore, protein overexpression of glutamic acid decarboxylase ($GAD_{65/67}$) and $GABA_A$ receptor subunits by western blot were found, being activated by SIN. In conclusion, SIN augments pentobarbital-induced sleeping behaviors through $GABA_A$-ergic systems, and increased NREM sleep. It could be a candidate for the treatment of insomnia.

방기(Sinomenium acutum)의 물추출물이 주요 장내 미생물의 생육에 미치는 영향

신옥호,유시승,이완규,신현경 한국산업미생물학회 1992 한국미생물·생명공학회지 Vol.20 No.5

한약재로부터 장내의 대표적 유해균인 Clostridium perfringes의 생육을 억제시키는 소재를 탐색한 결과 방기 물추출물에서 강력한 항균활성을 발견하였다. 방기 추출물의 항균활성은 pH 1부터 pH 13까지의 범위에서 안정하였으며 121℃에서 15분간의 열처리에서도 활성이 유지되었다. 방기 추출물은 Cl. Perfringens, Cl. Paraputrificum, Cl. ramosum 및 Cl. butyricum 등 실험에 사용한 모든 Clostridium속균의 생육을 강력하게 억제하였다. 그리고 Clostridium 이외의 주요 장내균에 대한 영향을 조사한 결과 Bif. bifidum, Bacteroides fragilis 및 Eubacterium limosum에 대해서는 역시 그 생육을 억제하였으나 Bif. adolescentis, Bif. infantis, Bif. longum, E. coli, Enterococcus faecalis 그리고 Staphylococcus aureus에 대해서는 생육억제 효과가 크게 나타나지 않았다. 반면 Lactobacillus acidophilus와 Bif. animalis에 대해서는 생육촉진효과를 보여주었다. The water extract of Sinomeniiacuti Radix (Sinomenium acutum) was found out to have a strong inhibition activity on the growth of Clostridium perfringens. The anti-bacterial activity was stable at the range of pH 1 to pH 13 and kept in a thermal treatment at 121℃ for 15 minutes. The minmal inhibition concentration of the Sinomeniiacuti Radix extract on the growth of Cl. perfringens was 1000 ppm. The Sinomeniiacuti Radix extract also suppressed the growth of Cl. ramosum, Cl. paraputrificum, Cl. butyricum, Bifidobacterium bificum, Bacteriodes fragilis, Eubacterium limosum. The extract, however, did not inhibit the growth of Bif. adolescentis, Bif. infantis. Bif. longum, E. coli, Enterococcus faecalis and Staphylococcus aureus. On the other hand, the extract showed a promoting effect for the growth of Bif. animalis and Lactobacillus acidophilus.

방기의 품질 평가를 위한 Sinomenine, Magnoflorine, Syringaresinol의 함량 분석

이지우,원진배,윤보라,엄민례,마충제 대한약학회 2013 약학회지 Vol.57 No.3

Sinomenium acutum has been used for the treatment of rheumatoid arthritis, arrhythmia, and pain. We eval-uated the quality of S. acutum extract by quantitatively analyzing its components such as sinomenine, magnoflorine and syringaresinol with the simultaneous determination method using HPLC-DAD. A total of 53 samples collected from dif- ferent localities were evaluated with this quality evaluation method. Sinomenine, magnoflorine and syringaresinol from tested samples ranged from 0.0649~9.1050%, 0.7460~10.7590% and 0.0010~0.2441%, respectively. In the current study, we were able to exhibit the diverse quality of S. acutum samples collected from various locations using the simultaneous determination method.

Anti-neuroinflammatory constituents from Sinomenium acutum rhizomes

Lee, S.Y.,Suh, W.S.,Cha, J.M.,Moon, E.,Ha, S.K.,Kim, S.Y.,Lee, K.R. Elsevier 2016 PHYTOCHEMISTRY LETTERS Vol.17 No.-

In our continuing search for bioactive constituents from Korean medicinal sources, an investigation of the Sinomenium acutum Rehder et Wilson rhizome afforded three new lignan derivatives (1@?3) together with nine known compounds (4@?12). The identification and structural elucidation of these new compounds were based on 1D and 2D-NMR (COSY, HMQC, HMBC and NOESY) and MS data. The absolute configurations were established based on the CD data. Nitric oxide production was evaluated in the BV-2 lipopolysaccharide-activated microglia cell line to investigate the anti-neuroinflammatory effects of the isolated compounds (1@?12).

Anti-osteoclastogenic effects of isoquinoline alkaloids from the rhizome extract of Sinomenium acutum

Ji Young Lee,Kwang-Jin Kim,Jinhee Kim,Sang Un Choi,Seong Hwan Kim,Shi Yong Ryu 대한약학회 2016 Archives of Pharmacal Research Vol.39 No.5

A phytochemical investigation for the rhizomeextract from Sinomenium acutum (Menispermaceae) resultedin the isolation of several active principles responsiblefor the anti-osteoclastogenic property of the extract, togetherwith related isoquinoline alkaloids (1–13) including twonew compounds, 1 and 2. Among isolated compounds,salutaridine (7), dauricumine (10), cheilanthifoline (12), anddauriporphine (13) were observed to give significant inhibitionson receptor activator of nuclear factor-jB ligandinduceddifferentiation of mouse bone marrow-derivedmacrophages into multinucleated osteoclasts, respectively. The chemical structures of two newly isolated compounds, 1and 2 were established as 8-demethoxycephatonine (1) and7(R)-7,8-dihydrosinomenine (2), by spectroscopic analysesincluding 2D NMR experiments.