The Effect of Irradiated Allogenic Cancellous Bone Combined with Tetracycline on Local Bone Formation in a Rat Calvarial Model

윤영주,강나라,김명래,김선종 한국조직공학과 재생의학회 2009 조직공학과 재생의학 Vol.6 No.13

The purpose of this study was to evaluate whether Irradiated Allogenic Cancellous combined with tetracycline could be effective on new bone formation in a rat calvarial defect model. Twenty-seven male Harland Sprague- Dawley rats were assigned to one of three treatment groups; Irradiated Allogenic Cancellous Bone, Irradiated Allogenic Cancellous Bone/Tetra-cycline and non-graft control. This material was placed into 8mm calvarial critical sized defects (CSDs). They were measured histologically and radiographically following a 2, 4 and 8 weeks healing interval. One way ANOVA test was used to compare the difference between the control and graft group. In the histomorphometric analysis, new bone area of the Irradiated Allogenic Cancellous Bone group was greater than that in the Irradiated Allogenic Cancellous Bone/Tetracycline group at 4 and 8 weeks, but difference was not statistically significant (p>0.05). Within the limitation of the results of this study, it is suggested that irradiated Allogenic Cancellous Bone is effective graft material on bone formation in the rat calvarial defect model and tetracycline does not enhance bone regeneration when it is combined with Irradiated Allogenic Cancellous. The purpose of this study was to evaluate whether Irradiated Allogenic Cancellous combined with tetracycline could be effective on new bone formation in a rat calvarial defect model. Twenty-seven male Harland Sprague- Dawley rats were assigned to one of three treatment groups; Irradiated Allogenic Cancellous Bone, Irradiated Allogenic Cancellous Bone/Tetra-cycline and non-graft control. This material was placed into 8mm calvarial critical sized defects (CSDs). They were measured histologically and radiographically following a 2, 4 and 8 weeks healing interval. One way ANOVA test was used to compare the difference between the control and graft group. In the histomorphometric analysis, new bone area of the Irradiated Allogenic Cancellous Bone group was greater than that in the Irradiated Allogenic Cancellous Bone/Tetracycline group at 4 and 8 weeks, but difference was not statistically significant (p>0.05). Within the limitation of the results of this study, it is suggested that irradiated Allogenic Cancellous Bone is effective graft material on bone formation in the rat calvarial defect model and tetracycline does not enhance bone regeneration when it is combined with Irradiated Allogenic Cancellous.

경골근위부 자가망상골 이식술

김철환(Chul Hwan Kim),이미향(Mi Hyang Lee),김미숙(Mi Suk Kim),김훈(Hoon Kim) 대한구강악안면외과학회 1997 대한구강악안면외과학회지 Vol.23 No.1

Many graft materials are available for surgical treatment of maxillofacial bony defect and deformity. Despite concerted efforts to gain comparable outcomes with allogenic bone, xenogenic bone, bone substitutes, and alloplasts, the results related to function, from and adaptibility remain superior with autogenous bone. The cancellous bone is relatively more osteogenic than cortical bone. The superior osteogenic properties of cancellous bone has been widely used an oral and maxillofacial surgery. Well-recognized donor sites of cancellous bone have included ilium, rib and calvarial bone, But the procurement of cancellous bone from any site should be no considered biologic insult to the patients. The proximal tibia as an alternative donor site has the potential to yield viable cancellous bone with a minimum of morbidity. We experienced several jaw defect treated with autogenous proximal tibial cancellous bone grafting. The harvested cancellous bone was soft and easily molded to fill defects and the recipient site showed a favorable result. Moreover, with uneventful healing process, the donor site showed the overall lack of morbidity. This report presents clinical applications of the autogenous cancellous bone graft from the proximal tibial metaphysis and satisfactory results to repair of the jaw defect.

A Two-Phase Medium Model for Predicting the Dependence of the Phase Velocity on the Porosity in Cancellous Bone

Kang Il Lee 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.52 No.2

Advances in modeling propagation of ultrasound in cancellous bone underpin the ability of ultrasound to diagnose bone health. The complexity has been increasing as such models seek to reflect to a greater degree the complexity in the human bone through which ultrasound propagates. This paper outlines an extremely simple model for ultrasonic propagation in cancellous bone, which is a two-phase medium in which the cancellous bone is modeled as a mixture of two fluid phases. This model requires four basic inputs, and it is shown here that these are the same four inputs that have been common to all models of ultrasonic propagation in cancellous bone to date. The application of this model to predict the phase velocity across the entire range of porosities (from 0 to 100 \%) indicates inherent constraints that may influence the prediction of complete models for a narrow range of porosities of typical human cancellous bone. This paper does not propose that the simple two-phase medium model replace other complex models; nevertheless, its use in identifying the main drivers, the constraints, and the commonalities among the suite of more comprehensive models is enlightening. Advances in modeling propagation of ultrasound in cancellous bone underpin the ability of ultrasound to diagnose bone health. The complexity has been increasing as such models seek to reflect to a greater degree the complexity in the human bone through which ultrasound propagates. This paper outlines an extremely simple model for ultrasonic propagation in cancellous bone, which is a two-phase medium in which the cancellous bone is modeled as a mixture of two fluid phases. This model requires four basic inputs, and it is shown here that these are the same four inputs that have been common to all models of ultrasonic propagation in cancellous bone to date. The application of this model to predict the phase velocity across the entire range of porosities (from 0 to 100 \%) indicates inherent constraints that may influence the prediction of complete models for a narrow range of porosities of typical human cancellous bone. This paper does not propose that the simple two-phase medium model replace other complex models; nevertheless, its use in identifying the main drivers, the constraints, and the commonalities among the suite of more comprehensive models is enlightening.

치조열 교정을 위한 장골 능선 해면골 재이식술

김석화,김병준,최태현 대한성형외과학회 2011 Archives of Plastic Surgery Vol.38 No.1

Purpose: The anterior iliac crest is a common source for autologous cancellous bone graft. For patients who have previously received cancellous bone grafts from bilateral anterior iliac crests, there may be concerns of whether a sufficient quantity of autologous cancellous bone remains for additional grafts without harvesting it from other sites, such as the posterior iliac crest. Methods: We experienced 3 cases of reharvesting in 2 patients. The diagnosis of the first patient was bilateral facial cleft number3. This patient received bilateral side cleft alveoloplasty with corticocancellous bone graft from the both anterior iliac crest respectively by a previous surgeon. This patient then needed to reharvest the anterior iliac crest cancellous bone to correct an ongoing skeletal problem for the bilateral cleft. The other patient had bilateral incomplete cleft of the primary palate. This patient received left side cleft alveoloplasty with cancellous bone graft from the right anterior iliac crest. Before the patient could receive the alveoloplasty on the other side, a radial head osteotomy and cancellous bone graft was performed by orthopedic surgeons who then used the remaining left iliac crest in order to treat a pulled elbow. For the completion of the right side cleft alveoplasty, the anterior iliac crest cancellous bone needed to be reharvested. Prior to the reharvesting, a preoperative computed tomography scan of the pelvis was obtained to assess the maturity of the donor site regeneration. The grafts were then taken from sites where a greater amount of regeneration was evident. Results: Long term follow ups showed that the grafts were successfully taken. This sufficient volume was obtainable 14 months after the first harvest. Conclusion: Satisfactory results were achieved after the reharvesting of iliac cancellous bone. Thus, it appears that the reharvesting of the iliac bone is a possible alternative to multiple site grafting, use of allograft or bone substitute materials.

The piezoelectricity of trabecular bone in cancellous bone wave propagation

Young June Yoon,Jae Pil Chung 한국정보전자통신기술학회 2021 한국정보전자통신기술학회논문지 Vol.14 No.1

The orientation of trabeculae and porosity determine the wave propagation in cancellous bone. Wave propagation, as well as charge density and piezoelectricity, stimulate bone remodeling. Also, Charged ions in the fluid affect wave propagation in cancellous bone. But the trabecular struts’ piezoelectricity does not change the waveform of cancellous bone. However, the underlying mechanism is unknown yet why trabecula struts" piezoelectricity does not change wave propagation through cancellous bone. Thus, we derived the governing equation indicating that trabecular struts" piezoelectric properties show that those do not affect wave propagation in cancellous bone.

Prediction of the Dependence of Phase Velocity on Porosity in Cancellous Bone

Lee, Kang-Il,Choi, Min-Joo The Acoustical Society of Korea 2008 韓國音響學會誌 Vol.27 No.e2

In recent years, quantitative ultrasound (QUS) technologies have played a growing role in the diagnosis of osteoporosis. Most of the commercial bone somometers measure speed of sound (SOS) and/or broadband ultrasonic attenuation (EUA) at peripheral skeletal sites. However, the QUS parameters are purely empirical measures that have not yet been firmly linked to physical parameters such as bone strength or porosity. In the present study, the theoretical models for wave propagation in cancellous bone, such as the Biot model, the stratified model, and the modified Biot-Attenborough (MBA) model, were applied to predict the dependence of phase velocity on porosity in cancellous bone. The optimum values for the input parameters of the three models in cancellous bone were determined by comparing the predictions with the previously published measurements in human cancellous bone in vitro. This modeling effort is relevant to the use of QUS in the diagnosis of osteoporosis because SOS is negatively correlated to the fracture risk of bone, and also advances our understanding of the relationship between phase velocity and porosity in cancellous bone.

방사선 조사 망상골이 외방형 골형성에 미치는 영향

정미현,허익,권영혁,박준봉,정종혁,Chung, Mi-Hyun,Herr, Yeek,Kwon, Young-Hyuk,Park, Joon-Bong,Chung, Jong-Hyuk 대한치주과학회 2007 Journal of Periodontal & Implant Science Vol.37 No.4

The purpose of this study was to evaluate exophytically vertical bone formation in residual ridge of the beagle dog by the concept of guided bone regeneration with a titanium reinforced e-PTFE membrane combined with irradiated cancellous human bone. Twelve male beagle dogs(mean age 1.5 years and mean weight 12kg) were used for this study. The alveolar ridges after extraction of all mandibular premolars were surgically and horizontally removed. At 8 weeks after extractions, full-thickness flap was reflected and cortical bone was removed with round bur and copious irrigation. Rectangular parallelepiped(10mm in length, 5mm in width, and 4mm in height) bended with titanium-reinforced e-PTFE(TR e-PTFE) membrane was placed on the decorticated alveolar ridge, fixed with metal pins and covered with full-thickness flap and assigned as a control group. Test groups ere treated with TR e-PTFE membrane filled with irradiated cancellous human bone. Of twelve beagle dogs, four control dogs and four test dogs without membrane exposure to oral cavity were sacrificed at 8 and 16 weeks respectively. The surgical sites were dissected out, fixed in 4% buffered formaldehyde, dyed using a Villanueva staining technique, and processed for embedding in plastic resin. The cutting and grinding methods were routinely processed for histologic and histomophometric analyis of exophytic bone formation as well as statistical analysis. The results of this study were as follows: 1. Exophytic bone formation in the both of experimental groups was increased respectively after surgery from 23.40% at 8 weeks to 46.26% at 16 weeks in the control groups, from 40.23% at 8 weeks to 47.11% at 16 weeks in the test groups(p<0.05). 2. At 8 weeks after surgery, exophytic bone formation was made 40.23% in the test groups and 33.40% in the control groups. Exophytic bone formation was significantly made in the test group more than in the control group. At 16 weeks after surgery, exophytic bone formation was made 44.11% in the test groups and 46.26% in the control groups. Exophytic bone formation was made in the test groups more than in the control groups, but there was no statistically significant differences. 3. The membrane was fixed with metal pins to closely contact it to the bone surface. So, collapse and deviation of the membrane could be prevented and in growth of connective tissue also could be blocked from the periphery of the membrane. On the basis of these findings, wee suggest that intraoral experimental model for exophytic bone formation may be effective to evaluate the effect of bone graft material. And it indicates that combined use of membrane and ICB graft material is more effective than use of membrane only for exophytic bone formation.

표면처리가 다른 5종 임플랜트의 안정성에 관한 연구

김선종,신상완,정성민,류재준,Kim Sun-Jong,Shin Sang-Wan,Jung Sung-Min,Ryu Jae-Jun 대한치과보철학회 2005 대한치과보철학회지 Vol.43 No.1

Purpose. The purpose of this study was to compare the effects of various surface treatments by measuring resonance frequency and histomorphometric analyses. Material and methods. In 5 adult dogs, the mandibular premolar were extracted. Six months later, 30 screw titanium implants (Dentium Co., Seoul, Korea) 6mm in length and 3.4mm in diameter, were placed in the mandibles of 5 dogs. Implants were divided into five groups following to surface treatment methods ; Group 1 is machined controls, Group 2 is sandblasted with large grit and acid-etched (SLA), Group 3 is anodized (Autoelectric Co., Korea, 660Hz, Duty10), Group 4 is hydroxyapatite(HA) coated by ion beam assisted deposition(E-beam), Group 5 is hydroxyapatite(HA) coated with Sol-gel coating process. Resonance frequency was measured implant placement immediately, and 3, 6 weeks and 10 weeks of healing perods. With the animal subject's sacrifice 10 weeks after implantation, implants were removed on bloc and histologic and computer-based histomorphometric analyses were performed. Histomorphometric analysis involved quantification of the entire bone to metal contact around the implants. Statistical analyses were performed using the SPSS for Windows (ver. 9.0 SPSS Inc.) Statistical differences were considered significant at P<0.05. Results. The results were as follows : 1) In five groups, mean value of resonance frequency analysis(RFA) were highest in group 5 (Sol-gel implant) at implantation and those of group 4 (E-beam)was highest at 10 weeks . but there was no correlation between surface treatments and RFA. 2) In all surface treatment groups, the RFA values of implants decreased until 3 weeks and increased to 10 weeks. 3) The percentage of direct bone-to-implant contact (BIC) had statistical significance between five groups in cancellous bone, (P<0.05) the percentage of bone density inside the thread had no statistical significance between five groups. (P>0.05) 4) There was a significant difference between cortical bone and cancellous bone in BIC. (P<0.05) and bone density. (P<0.05) 5) There was a correlation between the RFA value of implants at 10 weeks and BIC in cancellous bone, and between the RFA value of implants at 10 weeks and bone density in cortical bone. (P<0.05). Conclusions. These results indicate that surface treatment does not affect the implant stability in case of good bone quality.

성견 경골에서 자가장골이식이 임플란트 골유착에 미치는 영향

강남연,김명래,김선종 이화여자대학교 의과대학 2009 EMJ (Ewha medical journal) Vol.32 No.2

Objectives:This study was performed to evaluate the effect of autogenous iliac particulated marrow cancellous bone graft on osseointegration of implants in the dog tibia which has a loose bone marrow. Methods:Three mongrel dogs, weighing about15kg or more, and RBM(resorbable blasted media) AVANAⓡ implants(Osstem Inc., Korea) were used in this study. In the control groups, 2 implants sites were prepared conventionally with drills in the left metaphysic of tibia and 2 implants were placed in each dog under general anesthesia. In the experimental groups, 2 implant sites were prepared with drills. and the particulated marrow cancellous bone, which was obtained from the right ilium,was packed into both prepared sites with osteotomes in the right metaphysic of tib-ia in each dog. Two implants were placed. A total of 12 implants were placed in 3 dogs. The animals were sacrificed at 8 weeks after implant placement. The specimens were taken and embedded into the resin. H & E stain was done. The undecalcified specimens were prepared for histological examination and histomorphometric analysis of bone-implant contact ratios. The Mann-Whitney test was used to compare both groups.Values of p<0.01were considered statistically significant. Results:In the cross-sectional radiographs of the control group no trabecular bone was observed in the marrow space and the implant was surrounded with radiolucent marrow space. In the experimental group Some radioaque trabecular bone was observed in the marrow space of tibia and some of the trabecula was contacted with implant surface. Histologically,in the control group few trabecular bone was observed in marrow space and there was poor osseointegration in the marrow. In the experimental group most marrow space was filled with the trabecular boneand the implant surface was contacted with trabecular bone. In histomorphomeric analysis the bone-implant contact ratio of the experimental group(75.6%) was significantly higher than that of the control group(27.1%,p<0.01). Conclusion:The autogeneous bone graft into the loose bone marrow can be an option to improve the bone density and to get better osseointegration of dental implants.

Relationships of Bone Density with Quantitative Ultrasound Parameter in Bovine Cancellous Bone

이강일 한국물리학회 2009 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.54 No.1

The present study aims to investigate the relationships of bone density with quantitative ultrasound (QUS) parameters measured in transmission and backscatter modes in bovine cancellous bone. The speed of sound (SOS), the normalized broadband ultrasound attenuation (nBUA), the broadband ultrasound backscatter (BUB) and the integrated reflection coefficient (IRe) were measured for 35 specimens obtained from proximal ends of seven bovine tibiae. The highest correlation coefficient was observed for SOS (r = 0.75, p < 0.001). Similarly, nBUA was significantly correlated with density (r = 0.71, p < 0.001). In contrast, BUB and IRe were less correlated with density than transmission QUS parameters, but still significantly (r = 0.63 and 0.65, respectively, all p < 0.01). A multiple regression analysis was used to find optimal models for the prediction of apparent bone density by using linear combinations of SOS, nBUA, BUB and IRe, with density as an independent variable and 2,3 and 4 QUS parameters as dependent variables (r = 0.77 - 0.88, all p < 0.001). The results suggest that the analysis of multiple QUS parameters can contribute to improving the efficacy of QUS procedures in the diagnosis of osteoporosis beyond the established value of SOS and nBUA.