Optimization of bioethanol production from nigerian sugarcane juice using factorial design

Suleiman, Bilyaminu,Abdulkareem, Saka A.,Afolabi, Emmanuel A.,Musa, Umaru,Mohammed, Ibrahim A.,Eyikanmi, Tope A. Techno-Press 2016 Advances in energy research Vol.4 No.1

The quest to reduce the level of overdependence on fossil fuel product and to provide all required information on proven existing alternatives for renewable energy has resulted into rapid growth of research globally to identify efficient alternative renewable energy sources and the process technologies that are sustainable and environmentally friendly. The present study is aimed at production and characterization of bioethanol produced from sugarcane juice using a $2^4$ factorial design investigating the effect of four parameters (reaction temperature, time, concentration of bacteria used and amount of substrate). The optimum bioethanol yield of 19.3% was achieved at a reaction temperature of $30^{\circ}C$, time of 72 hours, yeast concentration of 2 g and 300 g concentration of substrate (sugarcane juice). The result of statistical analysis of variance shows that the concentration of yeast had the highest effect of 7.325 and % contribution of 82.72% while the substrate concentration had the lowest effect and % contribution of -0.25 and 0.096% respectively. The bioethanol produced was then characterized for some fuel properties such as flash point, specific gravity, cloud point, pour point, sulphur content, acidity, density and kinematic viscosity. The results of bioethanol characterization conform to American society for testing and materials (ASTM) standard. Hence, sugarcane juice is a good and sustainable feedstock for bioethanol production in Nigeria owing relative abundance, cheap source of supply and available land for large scale production.

Enhanced Biofuel Production from High-Concentration Bioethanol Wastewater by a Newly Isolated Heterotrophic Microalga, Chlorella vulgaris LAM-Q

( Tong Hui Xie ),( Jing Liu ),( Kai Feng Du ),( Bin Liang ),( Yong Kui Zhang ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.10

Microalgal biofuel production from wastewater has economic and environmental advantages. This article investigates the lipid production from high chemical oxygen demand (COD) bioethanol wastewater without dilution or additional nutrients, using a newly isolated heterotrophic microalga, Chlorella vulgaris LAM-Q. To enhance lipid accumulation, the combined effects of important operational parameters were studied via response surface methodology. The optimal conditions were found to be temperature of 22.8oC, initial pH of 6.7, and inoculum density of 1.2 × 108 cells/ml. Under these conditions, the lipid productivity reached 195.96 mg/l/d, which was markedly higher than previously reported values in similar systems. According to the fatty acid composition, the obtained lipids were suitable feedstock for biodiesel production. Meanwhile, 61.40% of COD, 51.24% of total nitrogen, and 58.76% of total phosphorus were removed from the bioethanol wastewater during microalgal growth. In addition, 19.17% of the energy contained in the wastewater was transferred to the microalgal biomass in the fermentation process. These findings suggest that C. vulgaris LAMQ can efficiently produce lipids from high-concentration bioethanol wastewater, and simultaneously performs wastewater treatment.

A Mild Thermal Pre-treatment of the Organic Fraction of Municipal Wastes Allows High Ethanol Production by Direct Solid-state Fermentation

R. Estrada-Martínez,E. Favela-Torres,N. O. Soto-Cruz,H. B. Escalona-Buendía,G. Saucedo-Castañeda 한국생물공학회 2019 Biotechnology and Bioprocess Engineering Vol.24 No.2

A solid standard mixture (SSM) representing the annual composition of fresh fruits and vegetables residues generated at the Supply Center in Mexico City was used for bioethanol production. This type of residues allows bioethanol production with a single thermal pretreatment instead of hard thermochemical or enzymatic treatments. The release of fermentable carbohydrates from the SSM by a mild thermal pretreatment was firstly optimized. After that, mixed and single cultures of Saccharomyces cerevisiae, Scheffersomyces stipitis, and Schwanniomyces occidentalis were evaluated for bioethanol production. The maximum ethanol production, 282.61 ± 13.09 L ethanol per ton of dry matter (DM), was reached using a severity factor (SF) of 2.35 and a mixed culture composed of Saccharomyces cerevisiae, Scheffersomyces stipitis, and Schwanniomyces occidentalis. The improved lab scale conditions were evaluated in a pilot scale (18 Kg) stirred bioreactor with an SF of 2.35 and the mixed culture, obtaining 245.72 ± 17.76 L ethanol per ton DM. The obtained results demonstrate for the first time the use of fresh fruits and vegetables residues for bioethanol production under solid-state culture conditions without any thermochemical or enzymatic pre-treatment.

Cellulosic bioethanol production from Jerusalem artichoke (<i>Helianthus tuberosus</i> L.) using hydrogen peroxide-acetic acid (HPAC) pretreatment

Song, Younho,Wi, Seung Gon,Kim, Ho Myeong,Bae, Hyeun-Jong Elsevier 2016 Bioresource technology Vol.214 No.-

<P><B>Abstract</B></P> <P>Jerusalem artichoke (JA) is recognized as a suitable candidate biomass crop for bioethanol production because it has a rapid growth rate and high biomass productivity. In this study, hydrogen peroxide-acetic acid (HPAC) pretreatment was used to enhance the enzymatic hydrolysis and to effectively remove the lignin of JA. With optimized enzyme doses, synergy was observed from the combination of three different enzymes (RUT-C30, pectinase, and xylanase) which provided a conversion rate was approximately 30% higher than the rate with from treatment with RUT-C30 alone. Fermentation of the JA hydrolyzates by <I>Saccharomyces cerevisiae</I> produced a fermentation yield of approximately 84%. Therefore, Jerusalem artichoke has potential as a bioenergy crop for bioethanol production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Jerusalem artichoke is a suitable candidate biomass crop for bioethanol production. </LI> <LI> Hydrogen peroxide-acetic acid (HPAC) pretreatment can effectively remove lignin. </LI> <LI> Optimized enzyme cocktails enhanced enzymatic hydrolysis of Jerusalem artichoke. </LI> <LI> Fermentation of the hydrolyzates with <I>S</I>. <I>cerevisiae</I> gave a fermentation yield of 84%. </LI> </UL> </P>

셀룰로오스계 원료작물로서 수수-수단그래스 교잡종의 바이오에탄올 생산량 평가

차영록,문윤호,구본철,안종웅,윤영미,남상식,김중곤,안기홍,박광근 韓國作物學會 2013 한국작물학회지 Vol.58 No.1

본 연구는 바이오에탄올 생산용 작물 선발을 위해 국내재배 가능한 일년생 작물중에서 바이오매스 생산량이 우수한 수수-수단그래스 교잡종에 대한 바이오에탄올 생산성을조사하였으며 그 결과는 다음과 같다.1. 총 11 품종의 수수-수단그래스 교잡종 대한 화학적 특성검증 결과 셀룰로오스 함량은 Green Star 품종이 가장높았으며 발효율 검정을 위해 Green Star, Revolution,KF429 그리고 SS504 4품종을 선발하였다.2. 선발된 4품종으로부터 발효 당을 추출하기 위해 시료와1 M NaOH 용매를 1:14의 비율로 혼합하고 150℃에서30분간 전처리하였을 때 시료내 셀룰로오스 함량은 55%이상 이였으며, 발효 저해 작용을 하는 리그닌 및 회분함량은 65%이상 제거 되었다.3. 전처리물의 당화율 검정을 위해 celli CTEC II 효소 30FPU/g-cellulose를 사용하였으며 4품종의 당화율은 평균86%이었다.4. 수수-수단그래스 교잡종의 발효율 검정은 동시당화발효(SSF)방법으로 수행하였으며 발효균주로는 Saccharomysiscerevisiae CHY1011를 사용하였고, 결과적으로 GreenStar의 발효율이 92.4%로 가장 높았으며 에탄올 생산량은 6,206 L/ha임을 확인하였다. The world demand of renewable bioenergy as an alternative transportation fuel is greatly increasing. Research for bioethanol production is currently being progressed intensively throughout the world. Therefore it will be necessary to develop bioethanol production with cellulosic materials. In this study, the yield of ethanol production was evaluated by simultaneous saccharification and fermentation (SSF) using sodium hydroxide pretreated sorghum x sudangrass hybrids. Composition analysis of 11 varieties of sorghum x sudangrass hybrids was performed for selection of excellent variety to efficiently produce bioethanol. The content of cellulose, hemicellulose, lignin and ash of these varieties were 32~39%, 19~24%, 17~22% and 6~11%, respectively. Among these varieties, 4 varieties of sorghum x sudangrass hybrids were selected for the evaluation of ethanol yield and those were pretreated with 1 M NaOH solution at 15 0℃ for 30 min using high temperature explosion system. After pretreatment, samples were neutralized with tap water. It contained 52~57% of cellulose. Simultaneous saccharification and fermentation (SSF) was carried out for 48 h at 33℃ by Saccharomyces cerevisiae CHY1011 using Green star variety. The yield of ethanol was 92.4% and the amount of ethanol production was estimated at 6206 L/ha.

모자반 가수분해물을 이용한 바이오 에탄올 생산

연지현(Ji-Hyeon Yeon),서현범(Hyeon-Beom Seo),오성호(Sung-Ho Oh),최원석(Won-Seok Choi),강도형(Do Hyung Kang),이현용(Hyeon-Yong Lee),정경환(Kyung-Hwan Jung) 한국생물공학회 2010 KSBB Journal Vol.25 No.3

We investigated the feasibility of bioethanol production from hydrolysate of brown seaweed Sargassum sagamianum. Prior to bioethanol production using yeasts, six yeast strains were compared and the best ones in terms of the ethanol production levels were selected. Pichiastipitis ATCC 7126, Pichia stipitis ATCC 58784, and Pichia stipitis ATCC 58376 were superior to others in terms of ethanol production. These yeast strains were used for producing bioethanol by the shaking bottle culture and the fermentor culture. Out of approximately 30 g/L reducing sugar, about 3~6 g/L and 4~7 g/L bioethanol were produced in the bottle culture and the fermentor one, respectively. Furthermore, it was observed that around 12~28 g-bioethanol was produced from 1 kilogram of Sargassum sagamianum. Compared with those previously published, these data were almost three to eight times higher in value.

Issues with increasing bioethanol productivity: A model directed study

Doraiswami Ramkrishna,Hyun-Seob Song 한국화학공학회 2010 Korean Journal of Chemical Engineering Vol.27 No.2

We explore a way to improve the efficiency of fermentation of lignocellulosic sugars (i.e., glucose and xylose) to bioethanol in a bioreactor. For this purpose, we employ the hybrid cybernetic model developed by Song et al. (Biotechnol and Bioeng, 103: 984-1000, 2009), which provides an accurate description on metabolism of recombinant S. cerevisiae due to its unique feature of accounting for cellular regulation. A comprehensive analysis of the model reveals many interesting features of the process whose balance is critical for increasing the productivity of bioethanol. In particular, the addition of extra xylose to the medium may increase ethanol productivity (a somewhat counterintuitive result as xylose metabolism is slower!), but one that must be orchestrated with control of other important variables. Effects of xylose addition are shown to be different for different reactor environments. In a batch culture, xylose addition substantially improves ethanol productivity at low sugar concentration (e.g., about 45% up by increasing initial xylose concentration from 10 to 30 g/L with glucose concentration of 20 g/L), but worsens it at high sugar concentration (e.g., about 10% drop by increasing xylose concentration from 40 to 160 g/L with glucose concentration of 80 g/L). On the other hand, the productivity of chemostats is constantly improved by increasing the ratio of xylose to glucose level in the feed. It is found that multiple local maxima can exist in chemostats and, consequently, optimal composition for mixed sugars is different depending on the allowable range of xylose addition. Batch operation, however, is found to be superior when mixed sugars are consumed slowly, while continuous operation becomes attractive for rapidly metabolized sugars such as pure glucose. Optimal reactor configurations for given lignocellulosic sugars are shown to depend on calculated operating curves. Reasonably close comparison of model simulations with existing batch fermentation data provides support in part to the value of the current effort. The lesson that emerges is the importance of modeling in improving the efficiency of bioprocesses.

Efficient Extraction of Bioethanol from Freshwater Cyanobacteria Using Supercritical Fluid Pretreatment

표동진,Taemin Kim,유지수 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.2

For the production of ethanol from freshwater cyanobacteria, a new pretreatment method using supercritical fluid was introduced. In this study, it was found that the supercritical fluid could penetrate inside the cell wall and help to liberate starch from cyanobacterial cells which resulted in the increase of the efficiency of ethanol production. For Microcystis aeruginosa, supercritical fluid pretreatment increased the amount of ethanol produced from cyanobacteria from 1.53 g/L to 2.66 g/L. For Anabaena variabilis, the amount of ethanol was increased from 1.25 g/L to 2.28 g/L. With use of supercritical fluid pretreatment, the efficiency of the process to obtain higher ethanol yields from freshwater cyanobacteria was improved upto 80%. The optimum temperature and pressure conditions for supercritical fluid pretreatment were determined as the temperature of 40 oC and the pressure of 120 atm. This study demonstrates the feasibility of using supercritical fluid pretreatment for ethanol production using freshwater cyanobacteria.

Efficient Extraction of Bioethanol from Freshwater Cyanobacteria Using Supercritical Fluid Pretreatment

Pyo, Dongjin,Kim, Taemin,Yoo, Jisun Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.2

For the production of ethanol from freshwater cyanobacteria, a new pretreatment method using supercritical fluid was introduced. In this study, it was found that the supercritical fluid could penetrate inside the cell wall and help to liberate starch from cyanobacterial cells which resulted in the increase of the efficiency of ethanol production. For Microcystis aeruginosa, supercritical fluid pretreatment increased the amount of ethanol produced from cyanobacteria from 1.53 g/L to 2.66 g/L. For Anabaena variabilis, the amount of ethanol was increased from 1.25 g/L to 2.28 g/L. With use of supercritical fluid pretreatment, the efficiency of the process to obtain higher ethanol yields from freshwater cyanobacteria was improved upto 80%. The optimum temperature and pressure conditions for supercritical fluid pretreatment were determined as the temperature of $40^{\circ}C$ and the pressure of 120 atm. This study demonstrates the feasibility of using supercritical fluid pretreatment for ethanol production using freshwater cyanobacteria.

Feasibility of Bioethanol Production from Cider Waste

( Lisandro G. Seluy ),( Raul N. Comelli ),( Maria T. Benzzo ),( Miguel A. Isla ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.9

Wastewater from cider factories (losses during transfers, products discarded due to quality policies, and products returned from the market) exhibits a Chemical Oxygen Demand greater than 170,000 mg O₂/l, mainly due to the ethanol content and carbohydrates that are added to obtain the finished product. These effluents can represent up to 10% of the volume of cider produced, and they must be treated to meet environmental regulations. In this work, a process was developed, based on alcoholic fermentation of the available carbohydrates present in ciders. The impact of inhibitors at different pH, size and reuse of inoculums and different nutrient supplementation on the ethanol yield were evaluated. The use of a 0.5 g/l yeast inoculum and corn steep water as the nutrient source allowed for depletion of the sugars in less than 48 h, which increased the content of ethanol to more than 70 g/l.