보문 : 톱밥재배에 의한 목이의 자실체 생산

조우식 ( Woo Sik Jo ),황억금 ( Eok Keum Hwang ),강민진 ( Min Jin Kang ),최성용 ( Seong Yong Choi ) 한국균학회 2010 韓國菌學會誌 Vol.38 No.1

목이버섯 추출물로부터 항혈전물질의 정제와 항혈전효과

박영서 ( Young Seo Park ),최혁준 ( Hyuk Joon Choi ),최태현 ( Tae Hyun Choi ) 한국산업식품공학회 2009 산업 식품공학 Vol.13 No.4

목이버섯으로부터 항혈전활성과 항혈소판응집활성을 지니는 물질을 추출하여 추출물의 혈행개선활성을 조사하였다. 건조 목이버섯을 0.1 N NaOH, methanol, ethanol 등의 용매를 사용하여 추출하여 각 추출물의 항혈전활성을 activated partial thromboplastin time, thrombin time과 prothrombin time 값으로 측정한 결과 methanol 용해성 분획이 각각 100, 124, 54 s로 조사한 분획 중에서 가장 높은 활성을 나타내었다. 활성 분획을 DEAE-Sepharose CL6B ion exchange column chromatography와 Sephacryl 400-HR gel permeation column chromatography로 정제하였으며 활성물질은 분자량이 150 kDa 이상인 다당류이며 mannose가 주요 구성당으로 되어 있는 xyloglucomannan의 복합다당체인 것으로 확인되었다. 정제된 다당류의 항혈전활성은 thrombin 활성을 저해하기 때문인 것으로 해석되었다. Blood coagulation and aggregation of platelet are crucial events in the pathogenesis of various ischemic diseases. The substance which can prevent blood coagulation and platelet aggregation was extracted from wood ear mushroom (Auricularia auricular-judae) and its anticoagulation activity was investigated. The dried A. auricular-judae was extracted with 0.1 N NaOH and its supernatant was further extracted with methanol and ethanol followed by H2O. The resulting methanol soluble fraction showed significant antithrombotic activity in activated partial thromboplastin time, thrombin time, and prothrombin time assays with values of 100, 124, and 54 sec, respectively. This active substance was purified with DEAE-Sepharose CL6B and Sephacryl 400-HR and was found to be polysaccharide with the average molecular weight of over 150 kDa. This polysaccharide was xyloglucomannan of which the main component was mannose, and its anticoagulant activity was mostly mediated by inhibition of thrombin activity.

목이버섯 추출물로부터 항혈전물질의 정제와 항혈전효과

박영서,최혁준,최태현 한국산업식품공학회 2009 산업 식품공학 Vol.13 No.4

목이버섯으로부터 항혈전활성과 항혈소판응집활성을 지니는 물질을 추출하여 추출물의 혈행개선활성을 조사하였다. 건조 목이버섯을 0.1 N NaOH, methanol, ethanol 등의 용매를 사용하여 추출하여 각 추출물의 항혈전활성을 activated partial thromboplastin time, thrombin time과 prothrombin time 값으로 측정한 결과 methanol 용해성 분획이 각각 100, 124, 54 s로 조사한 분획 중에서 가장 높은 활성을 나타내었다. 활성 분획을 DEAE-Sepharose CL6B ion exchange column chromatography와 Sephacryl 400-HR gel permeation column chromatography로 정제하였으며 활성물질은 분자량이 150 kDa 이상인 다당류이며 mannose가 주요 구성당으로 되어 있는 xyloglucomannan의 복합다당체인 것으로 확인되었다. 정제된 다당류의 항혈전활성은 thrombin 활성을 저해하기 때문인 것으로 해석되었다. Blood coagulation and aggregation of platelet are crucial events in the pathogenesis of various ischemic diseases. The substance which can prevent blood coagulation and platelet aggregation was extracted from wood ear mushroom (Auricularia auricular-judae) and its anticoagulation activity was investigated. The dried A. auricular-judae was extracted with 0.1 N NaOH and its supernatant was further extracted with methanol and ethanol followed by H2O. The resulting methanol soluble fraction showed significant antithrombotic activity in activated partial thromboplastin time, thrombin time, and prothrombin time assays with values of 100, 124, and 54 sec, respectively. This active substance was purified with DEAE-Sepharose CL6B and Sephacryl 400-HR and was found to be polysaccharide with the average molecular weight of over 150 kDa. This polysaccharide was xyloglucomannan of which the main component was mannose, and its anticoagulant activity was mostly mediated by inhibition of thrombin activity.

목이버섯으로부터 추출한 항혈전물질의 제품화와 동물실험을 통한 항혈전활성 검증

박영서 ( Young Seo Park ),최혁준 ( Hyuk Joon Choi ) 한국산업식품공학회 2010 산업 식품공학 Vol.14 No.4

Large-scale preparation steps of antithrombotic materials from wood ear mushroom (Auricularia auricular-judae) were established as follows. Grounded dry wood ear mushroom was extracted with 75% ethanol and its precipitate was extracted with 76℃ water for 2 hr followed by filter pressing. The filtrate was then concentrated by vacuum and extracted with 80% ethanol, and the resulting precipitate was then freeze-dried. The formula of the product was determined using consumer susceptibility tests as follows; mushroom extract 90.5%, high fructose corn syrup 2.0%, β-cyclodextrin 1.5%, fructo-oligosaccharide 2.0%, pear puree 4.0%. When the packed products were stored at 25, 37, or 45℃ for 8 weeks, there were no noticeable changes in water activity, moisture content, pH, and acidity. The viable cell number of total bacteria was slightly increased during the storage period at 25 and 37℃, The total bacteria were not detected in the product when stored at 45℃. When the product was injected intravenously into rat at the level of 1,000 mg/kg, antithrombotic activities such as activated partial thromboplastin time, thrombin time, prothrombin time, and FIB were increased when compared with the control group. When the product was administrated orally into rat at the level of 500 mg/kg, it showed the same antiplatelet activity to aspirin.

Research Notes : Development of Detection Methods for Cellulolytic Activity of Auricularia auricular-judae

( Woo Sik Jo ),( Soon Hwa Bae ),( Seung Yong Choi ),( So Deuk Park ),( Young Bok Yoo ),( Seung Chun Park ) 한국균학회 2010 Mycobiology Vol.38 No.1

목이버섯으로부터 추출한 항혈전물질의 제품화와 동물실험을 통한 항혈전활성 검증

박영서,최혁준 한국산업식품공학회 2010 산업 식품공학 Vol.14 No.4

목이버섯으로부터 항혈전물질을 추출하여 제품화하기 위한 대량생산공정을 다음과 같이 확립하였다. 즉, 건조된 목이버섯을 분쇄하여 75% ethanol로 추출하여 원심분리한 침전물에 증류수에 첨가한 후 76oC에서 2시간 추출한 후 압착여과하여 여과액을 얻는다. 이를 감압농축한 후 여기에 최종 농도가 80%가 되도록 ethanol을 첨가하여 추출한 침전물을 동결건조한다. 제품의 소비지 기호도 조사를 통해 부재료의 종류와 첨가량을 결정하여 배합비를 목이버섯 추출물 90.5%, 고과당 2.0%, β-cyclodextrin 1.5%, 올리고당 2.0%, 배 농축액 4.0%로 결정하였다. 제품을 포장하여 25, 37, 45oC에서 저장하였을 경우 저장 8주에서도 수분활성도, 수분함량, pH, 산도에 큰 변화가 없었다. 미생물 생균수는 25oC와 37oC에서 저장하였을 경우 저장기간이 지남에 따라 약간 증가한 반면, 45oC에서 저장하였을 경우에는 저장 2개월 후에는 대부분 사멸하였다. 제품을 쥐에 정맥투여하였을 경우 1,000 mg/kg 투여 시 APTT 활성, TT 활성, PT 활성, FIB 활성이 대조구와 비교하여 유의적으로 높았으며 500 mg/kg을 경구투여하였을 경우 항혈소판활성이 아스피린과 동일한 수준을 나타내었다. Large-scale preparation steps of antithrombotic materials from wood ear mushroom (Auricularia auricular-judae) were established as follows. Grounded dry wood ear mushroom was extracted with 75% ethanol and its precipitate was extracted with 76oC water for 2 hr followed by filter pressing. The filtrate was then concentrated by vacuum and extracted with 80% ethanol, and the resulting precipitate was then freeze-dried. The formula of the product was determined using consumer susceptibility tests as follows; mushroom extract 90.5%, high fructose corn syrup 2.0%, β-cyclodextrin 1.5%, fructo-oligosaccharide 2.0%, pear puree 4.0%. When the packed products were stored at 25, 37, or 45oC for 8 weeks, there were no noticeable changes in water activity, moisture content, pH, and acidity. The viable cell number of total bacteria was slightly increased during the storage period at 25 and 37oC, The total bacteria were not detected in the product when stored at 45oC. When the product was injected intravenously into rat at the level of 1,000 mg/kg, antithrombotic activities such as activated partial thromboplastin time, thrombin time, prothrombin time, and FIB were increased when compared with the control group. When the product was administrated orally into rat at the level of 500 mg/kg, it showed the same antiplatelet activity to aspirin.

목이버섯 분말 첨가 생면의 품질 특성

유현희 한국생활과학회 2019 한국생활과학회지 Vol.28 No.6

In this study, we assessed the quality characteristics and storage of wet noodlees containing various concentrations (0, 1, 3 and 5%, respectively) of Auricularia auricula-judae powder (AAP). Wet noodles of crude protein, crude ash, and moisture contents increased and crude lipid contents decreased according to the content of AAP. The weight, volume, and water absorption of cooked noodles increased, but the turbidity of the soup decreased with the addition of AAP. For Hunter’s color values of wet and cooked noodles, L ( lightness) and b (yellowness) values decreased with increasing concentration of AAP, whereas a (redness) value increased. Texture measurement indicated that springiness, chewiness, gumminess and cohesiveness values of the cooked noodles were the highest in the control group, and these parameters showed a tendency to decrease with increasing amounts of AAP, but adhesiveness increased. One percent of AAP had greater hardness value than the other samples. The pH of AAP noodle group was lower than that of the control group during storage. The wet noodles with AAP reduced total microbial counts compared with the control group during storage at 5oC for 21 days. From the sensory evaluation test, wet noodles with 1% and 3% of AAP were the best among all the samples studied. The color of 3% AAP was shown to be more favored than the other treatments. Thus, the results of this study suggest that AAP is a good ingredient for increasing the consumer acceptability and storage of wet noodles. 본 연구에서는 목이버섯 분말을 1%, 3%, 5%의 비율로 첨가하여 생면을 제조한 후 품질특성과 저장성, 관능특성을 측정하였다. 목이버섯 분말 첨가 생면은 조단백질, 조회분, 수분 함량이 대조군보다 유의적으로 높았고, 조지방은 낮게 나타났다. 생면 조리 후 중량, 부피수분흡수율은 목이버섯 분말 첨가량이 증가할수록 유의적으로 증가하였으며, 국물의 탁도는 유의적으로 감소하였다. 생면과 조리면 모두 대조군에 비해 목이버섯 분말의 첨가량이 증가함에 따라 명도와 황색도는 유의적으로 감소하였으며, 적색도는 유의적으로 증가하였다. 조직감 측정 결과 생면의 경도는 대조군이 가장 낮았고 목이버섯 분말 첨가량이 증가함에 따라 증가하였으며, 탄력성, 씹힙성, 검성, 응집성은 대조군이 가장 높았고 목이버섯 분말 첨가량이 증가함에 따라 감소하였으며, 부착성은 1% 첨가군이 대조군과 3%와 5% 첨가군보다 높았다. 조리면 경도는 1% 첨가군이 대조군과 3%와 5% 첨가군보다 높았으며, 부착성은 대조군이 가장 낮고 목이버섯 분말 첨가량이 증가할수록 증가하였으며, 탄력성, 씹힙성, 검성, 응집성은 대조군이 가장 높고 목이버섯 분말 첨가량이 증가할수록 감소하였다. 저장기간 동안 목이버섯 분말 첨가량이 많을수록 pH의 감소와 총균수 증가를 지연하였다. 조리면의 색, 향미, 전체적 기호도는 대조군보다 1%와 3% 첨가군이 모두 유의적으로 높았으며, 조직감은 1% 첨가군이, 색, 향미, 맛, 외관, 전반적인 기호도는 3% 첨가군이 가장 높았다. 특히 색의 기호도가 3% 첨가군이 다른 관능검사 항목보다 높은 값을 보였다. 이로보다 목이버섯 분말을 첨가하여 생면을 제조 하면 저장성이 향상될 것으로 보이며 기호도 면에서 3%정도 첨가하는 것이 바람직할 것으로 보인다.

목이버섯 배지 오염 곰팡이균의 분리, 동정 및 생물학적 방제제 선발

김서연,조미주,안선민,박지윤,박지원,홍성국,김지우,차주훈,노유진,김다솜,전미진,지원재,박숙영,Seoyeon Kim,Miju Jo,Sunmin An,Jiyoon Park,Jiwon Park,Sungkook Hong,Jiwoo Kim,Juhoon Cha,Yujin Roh,Da Som Kim,Mi jin Jeon,Won-Jae Chi,Sook-Young Park 한국식물병리학회 2024 식물병연구 Vol.30 No.1

Black wood ear mushroom (Auricularia auricula-judae) is one of the most economically important mushrooms in China, Japan, and Korea. The cultivation of wood ear mushrooms on artificial substrates is more efficient in terms of time and cost compared with their natural growth on trees. However, if the substrate cultivation is infected by fast-growing fungi, the relatively slow-growing ear mushroom will be outcompeted, leading to economic losses. In this study, we investigated the competitive fungal isolates from substrates infected with fast-growing fungi for the cultivation of ear mushrooms in Jangheung and Sunchon, Korea. We collected 54 isolates and identified them by sequencing their internal transcribed spacer region with morphological identification. Among the isolates, the dominant isolates were Trichoderma spp. (92.6%), Penicillium spp. (5.6%), and Talaromyces sp. (1.8%). To find an appropriate eco-friendly biocontrol agent, we used five Streptomyces spp. and Benomyl, as controls against Trichoderma spp. and Penicillium spp. Among the six Streptomyces spp., Streptomyces sp. JC203-3 effectively controlled the fungi Trichoderma spp. and Penicillium spp., which pose a significant problem for the substrates of black wood ear mushrooms. This result indicated that this Streptomyces sp. JC203-3 can be used as biocontrol agents to protect against Trichoderma and Penicillium spp.