아까시꽃, 송지 및 토판염을 이용한 구강세정제의 항산화 및 항균 활성

윤석희,한정순,김애정 한국피부과학연구원 2017 아시안뷰티화장품학술지 Vol.15 No.2

목적: 본 연구는 동결건조 아까시꽃 열수추출물을 송지 및 토판염과 혼합하여 제조한 구강세정제의 항산화 활성과 항균 활성을 측정하여 천연 구강세정제로서의 가능성을 탐색하고자 수행되었다. 방법: 아까시꽃을 동결건조, 덖음건조, 열풍건조 방법으로 항산화 활성을 1,1-diphenyl-2-picrylhydrazyl (DPPH) 라디칼 소거능과 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) 라디칼 소거능으로 측정하였다. 구강세정제의 항산화 활성은 DPPH 라디칼 소거능으로 측정하였다. 항균 활성은 확산한천법에 의해 평가하였다. 결과: 아까시꽃은 동결건조하였을 때 가장 항산화 활성이 우수하였다. 동결건조 아까시꽃 열수추출물의 첨가 함유량이 증가할수록 항산화 활성이 증가하였다. 항균 활성도 동결건조 아까시꽃 열수추출물의 첨가량이 증가할수록 높게 나타났다. 결론: 이상의 결과 동결건조 아까시꽃 열수추출물, 송지 및 토판염을 이용하여 제조한 구강세정제는 항산화 및 항균 효과를 나타내어 구강위생관리를 위한 천연 구강세정제로서의 가능성을 기대할 수 있을 것으로 사료된다. Purpose: This study investigated the anti-oxidant and anti-bacterial activities of mouthwash prepared using freeze dried acacia flower hot water extracts (FDAFWEx), including songji and topan solar salt, and created a natural mouthwash. Methods: The anti-oxidant activity of acacia flowers was measured by the radical scavenging activities of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) using freeze drying, roasting, and hot air drying. The anti-oxidant activity was estimated using the radical scavenging activity of the DPPH in the mouthwash. The anti-bacterial activity was evaluated using the paper disk diffusion method. Results: The greatest anti-oxidant activity of acacia flowers was observed in the FDAFWEx. The anti-oxidant activity of mouthwash was increased by adding FDAFWEx in a dose-dependent manner. The anti-bacterial activity was increased by including the FDAFWEx content. Conclusion: FDAFWEx was determined to have anti-oxidant and anti-bacterial activities, and it is anticipated that mouthwash containing FDAFWEx could be a natural mouthwash.

대복피 추출물의 분획별 항균 및 항산화 활성

송선영,이현화 한국피부과학연구원 2016 아시안뷰티화장품학술지 Vol.14 No.4

Purpose: This present study was performed to investigate anti-oxidant and anti-bacterial activities of Arecae pericarpium MeOH extracts and its solvent fractions. Methods: Arecae pericarpium was extracted by 80% MeOH and fractionated by n-hexane, ethyl acetate (EtOAc), n-butanol, and H2O. Free radical scavenging activities on 80% MeOH Arecae pericarpium extracts and its fractions were evaluated by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. Anti-oxidant effects were confirmed by catalase (CAT) and superoxide dismutase (SOD) activities. Anti-bacterial activity was evaluated by the paper disk diffusion method. Results: Extraction yields of 80% MeOH Arecae pericarpium extracts and its fractions such as n-hexane, EtOAc, n-butanol, and H2O, were 19.04, 14.33, 12.07, 22.53, and 50.97%, respectively. The 80% MeOH extracts got the highest value (16.6 mg/g) in total polyphenol contents. In the case of DPPH free radical scavenging activity, the n-hexane fraction was 102.9 mg/mL. It was similar to the value of positive control, vitamin C and butylated hydroxytoluene (BHT). The highest values of SOD and CAT activities were confirmed at the EtOAc fraction (38.73 U mg protein-1) and the n-butanol fraction (2.75 U mg protein-1 min-1), respectively. In the results of anti-bacterial activity, 80% MeOH Arecae pericarpium extracts had anti-bacterial effects on all eight microorganisms. The n-hexane fraction had the anti-bacterial effects on only Listeria monocytogenes. There are no anti-bacterial effects on the other fractions such as EtOAc, n-butanol, and H2O. Conclusion: It is considered that these results imply the 80% MeOH Arecae pericarpium extracts can be used as not only natural antioxidants but also natural antibacterials. 목적: 본 연구는 대복피 메탄올(methanol, MeOH) 추출물 및 용매 분획물의 항산화 및 항균활성을 확인하고자 수행되었다. 방법: 대복피를 80% MeOH로 추출하고, n-hexane, ethyl acetate (EtOAc), n-butanol, H2O로 분획하였다. 추출물과 분획물의추출 수율, 총 폴리페놀 함량을 측정하였다. 대복피의 80% MeOH 추출물과 용매 분획물의 free radical 소거능은 1,1-diphenyl- 2-picrylhydrazyl (DPPH) 방법에 의해 평가하였다. 항산화 활성은 superoxide dismutase (SOD) 및 catalase (CAT) 활성으로 평가하였다. 항균활성은 디스크 확산법에 의해 평가하였다. 결과: 대복피 80% MeOH 추출물 및 n-hexane, EtOAc, n-butanol, H2O 용매 분획물의 추출 수율의 결과, 각각 19.04, 14.33, 12.07, 22.53, 50.97%로 측정되었다. 총 폴리페놀 함량은 80% MeOH 추출물에서 16.6 mg/g으로 가장 높게 측정되었다. DPPH free radical 소거능은 n-hexane 분획물에서 102.9 mg/mL로 양성대조군인 vitamin C 및 butylated hydroxytoluene (BHT)와 비슷한 우수한 항산화 활성을 나타내었다. SOD 활성은 EtOAc 분획물에서가장 높은 값(38.73 U mg protein-1)을 나타내었다. CAT 활성은 n-butanol 분획물에서 가장 높은 값(2.75 U mg protein-1 min-1) 을 나타내었다. 항균활성의 결과에서, 대복피 80% MeOH 추출물은 8종의 균주에 대해 항균활성을 나타냈다.- nHexane 분획물은오직 Listeria monocytogenes에 대해서만 항균활성을 나타냈다. EtOAc, n-butanol, H2O 분획물에서는 8종 균주에 대하여 항균활성을 나타내지 않았다. 결론: 이상의 결과에서 대복피 80% MeOH 추출물은 천연 항산화 및 항균 소재로서의 활용이 가능할 것으로 기대된다.

당근 지상부 추출물 유래 항염 및 항균 활성 성분

김정은 ( Jung Eun Kim ),조연정 ( Yeon Jeong Jo ),이남호 ( Nam Ho Lee ) 대한화장품학회 2018 대한화장품학회지 Vol.44 No.4

본 연구에서는 당근 지상부 추출물 및 용매 분획물의 항염, 항균 활성을 확인하고 유효성분을 분리하여 화학구조를 동정하였다. 당근 지상부 에탄올 추출물 및 용매 분획물의 항염 활성을 측정하기 위해 LPS로 자극된 RAW 264.7 세포를 이용하여 NO 생성 억제 활성을 확인하였다. 그 중 에틸아세테이트 분획물이 NO의 생성을 농도 의존적으로 감소시키는 효과가 나타났고, 추가적인 항염 기전 연구를 위해 에틸아세테이트 분획물에 대해 PGE₂, 전염증성 사이토카인의 생성량 및 iNOS, COX-2 단백질의 발현량을 측정하였다. 그 결과, 에틸아세테이트 분획물이 PGE₂, IL-1β, IL-6의 생성을 감소시키고, iNOS, COX-2 단백질의 발현도 억제 시키는 효과가 있음을 확인하였다. 또한 표피포도상구균과 여드름균을 이용한 활성 실험 결과, 헥산 및 에틸아세테이트 분획물에서 항균 활성이 나타났다. 이와 같은 결과를 바탕으로 에틸아세테이트 분획물에 대해 컬럼 크로마토그래피를 수행하여 3개의 화합물을 분리하였으며, ¹H 및 ¹³C NMR 데이터 분석과 문헌을 통하여 화학구조를 동정하였다; diosmetin (1), disomin (2), cynaroside (3). 분리된 화합물 1-3에 대해 항염 및 항균 활성을 측정하였으며, 그 결과 화합물 1-3 모두 NO의 생성을 저해시키는 효과가 있음을 확인하였다. 또한 화합물 3은 여드름균에 대해 항균 활성이 우수하였다. 이상의 연구 결과를 바탕으로 당근 지상부 추출물을 이용한 항염 및 항균 효과를 갖는 천연 화장품 소재로의 개발이 가능할 것이라 사료된다. In this study, we investigated anti-inflammatory and anti-bacterial constituents from Daucus carota var. sativa (carrot) areal parts. For the extract and solvent fractions, the anti-inflammatory activities were examined by measuring the nitric oxide (NO) production using LPS-stimulated RAW 264.7 cells. Among them, the ethyl acetate (EtOAc) fraction decreased the NO level in a dose-dependent manner. To elucidate further anti-inflammatory mechanisms, EtOAc fraction was evaluated by estimating their effects on the production of prostaglandin E2 and pro-inflammatory cytokines as well as on the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). As a result, the EtOAc fraction was determined to inhibit the production of PGE2, IL-1β, IL-6 and reduce the iNOS, COX-2 protein expression. Upon the anti-bacterial tests using Staphylococcus epidermidis and Propionibacterium acnes, n-hexane (Hex) and EtOAc fractions showed the most potent activities. Three phytochemicals were isolated form the EtOAc fraction; diosmetin (1), diosmin (2), cynaroside (3). The chemical structures of the isolated compounds were elucidated based on the spectroscopic data including 1H and 13C NMR spectra, as well as comparison of the data to the literatures. Anti-inflammatory and anti-bacterial effects were studied for the isolates. All of the compounds (1 - 3) decreased the NO production, effectively. Also, compound 3 showed anti-bacterial activity on P. acnes. Based on these results, D. carota var. sativa extract could be potentially applicable as anti-inflammatory and anti-bacterial ingredients in cosmetic formulations.

수확시기별 단풍나무 추출물의 항산화 활성과 항균 활성

성영환,장민주,최용근,송학진,심석영,윤재윤,김광진,김형주 한국피부과학연구원 2019 아시안뷰티화장품학술지 Vol.17 No.4

목적: 본 연구는 수확시기별 단풍나무 추출물의 항산화 활성과 항균 활성을 비교하여, 생리활성이 높은 적정 수확시기 및 이용시기를 도출하고자 하였다. 방법: 단풍나무 잎을 수확 시기별로 추출한 후, 총 폴리페놀 함량, 총 플라보노이드 함량, DPPH radical 소거활성, 세포독성, 그리고 Staphylococcus aureus, Bacillus subtilis 그리고 Pseudomonas aeruginosa에 대해 항균 활성을 측정하였다. 결과: 단풍나무 추출물의 항산화 함량과 항산화 활성은 7월과 11월에 가장 높게 나타났다. RAW 264.7 세포에 대해서 7월과 11월의 시료 5-100 µg/mL 농도에서 높은 세포생존율을 나타냈다. 3종의 균주에 대한 항균 실험 결과, 시기별로 항균 활성에 차이를 나타냈다. 결론: 단풍나무 추출물은 항산화 함량, 항산화 활성 및 항균 활성에서 시기별 차이를 보였으며, 항산화능이 높은 7월과 11 월의 100 µg/mL 농도에서 높은 세포생존율을 보였다. 따라서 단풍나무 추출물을 천연 항산화제로서 활용할 경우 수확시기는 중요한 요소로 작용하는 것을 확인할 수 있었으며 7월과 11월이 최적의 수확 시기임을 확인하였다. Purpose: This study aimed to investigate the optimal collection time of Acer palmatum extract (APE) through anti-oxidant and anti-bacterial activities. Methods: We confirmed the anti-oxidant activity of APE by estimating the total polyphenol content, total flavonoid content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging effect, and cell viability. In addition, we determined anti-bacterial activities against Staphylococcus aureus, Bacillus subtilis, and Pseudomonas aeruginosa during growth. Results: Anti-oxidant activities of APE peaked in July and November. RAW 264.7 displayed high cell viability in APE concentrations of 5–100 µg/mL in July and November. As a result of conducting anti-bacterial activities, seasonal variation of APE against three bacteria was exhibited. Conclusion: The anti-oxidant and anti-bacterial activities of APE showed seasonal variation, and high cell viability in July and November; therefore, consideration of the optimal seasonal collection time for Acer palmatum could be an important factor attributing to its natural antioxidant properties and the optimal collection time was confirmed especially to be in July and November.

등수국 잎 추출물 유래 항염 및 항균 활성 성분

조성미 ( Seong Mi Jo ),김정은 ( Jung Eun Kim ),이남호 ( Nam Ho Lee ) 대한화장품학회 2020 대한화장품학회지 Vol.46 No.3

본 연구에서는 등수국 잎 추출물의 항염 및 항균 활성을 확인하고 유효성분을 분리하여 화학구조를 동정하였다. RAW 264.7 세포를 이용한 항염 활성 실험 결과, n-hexane (Hex) 및 ethyl acetate (EtOAc) 분획물이 세포 독성 없이 nitric oxide (NO)의 생성 및 iNOS 단백질의 발현을 농도의존적으로 억제시키는 것을 확인하였다. 추가적인 항염 기전 연구 결과, n-Hex 및 EtOAc 분획물이 전염증성 cytokine (TNF-α, IL-1β, IL-6)의 생성을 억제하는 것을 확인하였다. 또한 표피포도상구균과 여드름균을 이용한 활성 실험결과, 추출물 및 n-Hex, EtOAc, n-butanol (BuOH) 분획물에서 항균 활성이 나타났다. n-Hex 및 EtOAc 분획물의 활성 성분을 규명하기 위해 컬럼 크로마토그래피를 수행하여 4 개의 화합물을 분리하였다; phytol (1), corosolic acid (2), asiatic acid (3) 및 1-O-p-coumaroyl-β-D-glucopyranoside (4). 이들 화합물은 모두 등수국에서 처음으로 분리된 물질이다. 또한 HPLC 분석을 통해 등수국 잎 추출물에서 분리된 화합물의 함량을 측정한 결과 phytol (1) 이 27.8 mg/g 함유되어 있는 것으로 확인되었다. 이상의 연구 결과를 바탕으로 등수국 잎 추출물을 이용한 항염 및 항균 효과를 갖는 천연 화장품 소재로의 개발이 가능할 것이라 사료된다. In this study, the anti-inflammatory and anti-bacterial activities of the extracts from the leaves of the Hydrangea petiolaris were identified, and the chemical structure was identified by separating the active ingredient. As the result of the anti-inflammatory activity experiment using RAW 264.7 cells, it was confirmed that the n-hexane (Hex) and ethyl acetate (EtOAc) fractions inhibited the production of nitric oxide (NO) and the expression of iNOS protein in a concentration-dependent manner without cytotoxicity. In addition, the n-Hex and EtOAc fractions reduced the production of pro-inflammatory cytokines (TNF-α, IL-1β and IL-6). Upon the anti-bacterial tests using Staphylococcus epidermidis and Cutibacterium acnes, the extract, n-Hex, EtOAc and n-butanol (BuOH) fractions showed potent activities. In order to isolate the active constituents, the n-Hex and EtOAc fractions were further purified to afford four phytochemicals; phytol (1), corosolic acid (2), asiatic acid (3) and 1-O-p-coumaroyl-β-D-glucopyranoside (4). All of the compounds 1 - 4 were isolated for the first time from this plant. In addition, the contents of isolated compounds were determined by HPLC and the quantity of phytol (1) was 27.8 mg/g for the 70% EtOH extract. Based on the above research results, it is believed that it will be possible to develop a natural cosmetic material that has anti-inflammatory and anti-bacterial effects using the extract of H. petiolaris leaves.

In-Vitro, Anti-Bacterial Activities of Aqueous Extracts of Acacia catechu (L.F.)Willd, Castanea sativa, Ephedra sinica stapf and shilajita mumiyo Against Gram Positive and Gram Negative Bacteria

Dashtdar, Mehrab,Dashtdar, Mohammad Reza,Dashtdar, Babak,shirazi, Mohammad khabaz,Khan, Saeed Ahmad KOREAN PHARMACOPUNCTURE INSTITUTE 2013 Journal of pharmacopuncture Vol.16 No.2

Objective: Evaluations of the in-vitro anti-bacterial activities of aqueous extracts of Acacia catechu (L.F.)Willd, Castanea sativa, Ephedra sinica stapf and Shilajita mumiyo against gram-positive bacteria (Staphylococcus aureus, Streptococcus pneumonia) and gram-negative bacteria (Escherichia coli, klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa) are reasonable since these ethnomedicinal plants have been used in Persian folk medicine for treating skin diseases, venereal diseases, respiratory problems and nervous disorders for ages. Methods: The well diffusion method (KB testing) with a concentration of $250{\mu}g/disc$ was used for evaluating the minimal inhibitory concentrations (MIC). Maximum synergistic effects of different combinations of components were also observed. Results: A particular combination of Acacia catechu (L.F.) Willd, Castanea sativa, Ephedra sinica stapf and shilajita mumiyo extracts possesses an outstanding anti-bacterial activity. It's inhibiting effect on microorganisms is significant when compared to the control group (P<0.05). Staphylococcus aureus was the most sensitive microorganism. The highest anti-bacterial activity against gram-positive bacteria (Staphylococcus aureus, Streptococcus pneumonia) or gram-negative bacteria (Escherichia coli, Klebsiella pneumonia, Proteus mirabilis, and Pseudomonas aeruginosa) was exerted by formula number 2 (table 1). Conclusion: The results reveal the presence of anti-bacterial activities of Acacia catechu, Castanea sativa husk, Ephedra sp. and Mumiyo against gram-positive and gram-negative bacteria. Synergistic effects in a combined formula, especially in formula number 2 (ASLAN$^{(R)}$) can lead to potential sources of new antiseptic agents for treatment of acute or chronic skin ulcers. These results considering the significant anti-bacterial effect of the present formulation, support ethnopharmacological uses against diarrheal and venereal diseases and demonstrate use of these plants to treat infectious diseases.

참꽃나무 잎 추출물 유래 항산화 및 항균 활성 성분

김정은 ( Jung Eun Kim ),조성미 ( Seong Mi Jo ),이남호 ( Nam Ho Lee ) 대한화장품학회 2019 대한화장품학회지 Vol.45 No.4

본 연구에서는 참꽃나무 잎 추출물의 항산화 및 항균 활성을 확인하고 유효성분을 분리하여 화학구조를 동정하였다. 참꽃나무 잎 에탄올 추출물 및 용매 분획물의 DPPH, ABTS⁺ 라디칼 소거 활성을 측정한 결과 추출물 및 ethyl acetate (EtOAc), n-butanol (BuOH) 분획물의 우수한 라디칼 소거 활성을 확인하였다. 또한 표피포도상구균과 여드름균을 이용한 활성 실험 결과, 추출물 및 n-hexane (Hex), EtOAc 분획물에서 항균 활성이 나타났다. EtOAc 분획물의 활성 성분을 규명하기 위해 컬럼 크로마토그래피를 수행하여 5개의 화합물을 분리하였다; ursolic acid (1), corosolic acid (2), asiatic acid (3), astragalin (4), isoquercetin (5). 분리된 화합물 1-5는 모두 참꽃나무에서 처음으로 분리되었다. 분리된 화합물에 대한 항산화 활성 측정 결과 화합물 4, 5의 라디칼 소거 활성이 우수하였으며, 화합물 3은 항균 효과가 있음을 확인하였다. 또한 UPLC 분석을 통해 참꽃나무 잎에서 분리된 astragalin의 함량을 측정한 결과 추출물에서 8.1 mg/g, EtOAc 분획물에서 34.8 mg/g이 함유되어 있는 것으로 확인되었다. 이상의 연구 결과를 바탕으로 참꽃나무 잎 추출물을 이용한 항산화 및 항균 효과를 갖는 천연 화장품 소재로의 개발이 가능할 것이라 사료된다. In this study, we investigated anti-oxidative and anti-bacterial constituents from Rhododendron weyrichii leaves. DPPH and ABTS⁺ radical scavenging activities were screened for the ethanol extract and solvent fractions. Potent scavenging activities were appeared from the extract, ethyl acetate (EtOAc) and n-butanol (BuOH) fractions. Upon the anti-bacterial tests using Staphylococcus epidermidis and Propionibacterium acnes, extract, n-hexane (Hex) and EtOAc fractions showed strong activities. To isolate the active constituents, the EtOAc fraction was further purified to afford five phytochemicals; ursolic acid (1), corosolic acid (2), asiatic acid (3), astragalin (4) and isoquercetin (5). All of the compounds 1-5 were isolated for the first time from this plant. Among the isolates, the compound 4 and 5 showed strong DPPH and ABTS⁺ radical scavenging activities. Also, compound 3 exhibited the most potent anti-bacterial activity. In addition, the content of astragalin isolated from this plant was determined by UPLC and the quantity was about 8.1 mg/g for the 70% ethanol extract and 34.8 mg/g for the EtOAc fraction. Based on these results, it is concluded that R. weyrichii extract could be potentially applicable as anti-oxidative and anti-bacterial ingredients in cosmetic formulations.

In-Vitro, Anti-Bacterial Activities of Aqueous Extracts of Acacia catechu (L.F.)Willd, Castanea sativa, Ephedra sinica stapf and shilajita mumiyo Against Gram Positive and Gram Negative Bacteria

Mehrab Dashtdar,Mohammad Reza Dashtdar,Babak Dashtdar,Mohammad khabaz shirazi,Saeed Ahmad Khan 대한약침학회 2013 Journal of pharmacopuncture Vol.16 No.2

Objective: Evaluations of the in-vitro anti-bacterial activities of aqueous extracts of Acacia catechu (L.F.)Willd, Castanea sativa, Ephedra sinica stapf and Shilajita mumiyo against gram-positive bacteria (Staphylococcus aureus, Streptococcus pneumonia) and gram-negative bacteria (Escherichia coli, klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa) are reasonable since these ethnomedicinal plants have been used in Persian folk medicine for treating skin diseases, venereal diseases, respiratory problems and nervous disorders for ages. Methods: The well diffusion method (KB testing) with a concentration of 250 μg/disc was used for evaluating the minimal inhibitory concentrations (MIC). Maximum synergistic effects of different combinations of components were also observed. Results: A particular combination of Acacia catechu (L.F.) Willd, Castanea sativa, Ephedra sinica stapf and shilajita mumiyo extracts possesses an outstanding anti-bacterial activity. It's inhibiting effect on microorganisms is significant when compared to the control group (P < 0.05). Staphylococcus aureus was the most sensitive microorganism. The highest antibacterial activity against gram-positive bacteria (Staphylococcus aureus, Streptococcus pneumonia) or gram-negative bacteria (Escherichia coli, Klebsiella pneumonia, Proteus mirabilis, and Pseudomonas aeruginosa) was exerted by formula number 2 (table 1). Conclusion: The results reveal the presence of antibacterial activities of Acacia catechu, Castanea sativa husk, Ephedra sp. and Mumiyo against gram-positive and gram-negative bacteria. Synergistic effects in a combined formula, especially in formula number 2(ASLANⓇ) can lead to potential sources of new antiseptic agents for treatment of acute or chronic skin ulcers. These results considering the significant antibacterial effect of the present formulation, support ethnopharmacological uses against diarrheal and venereal diseases and demonstrate use of these plants to treat infectious diseases.

Anti-bacterial Activities of Essential Oil Obtained from Hydrodistillation of Artemisia princeps var. orientalis

최윤경,하병조 대한미용학회 2014 대한미용학회지 Vol.10 No.2

This experimental study reported the antibacterial activities of the essential oil obtained from Artemisia princeps var. orientalis from Kimpo, Gyunggi-do province (Korea). The yield of essential oil, collected by hydrodistillation, was0.02% w/w. The 1% solution of essential oil showed anti-bacterial activities about Staphylococcus aures, Escherichiacoli, and Streptococuus mutans. Diameter of zone of inhibition using paper disk diffusion method showed 12 mm and 16mm, respectively. However, it did not show anti-bacterial activity about Escherichia coli. By comparison, 0.3% solutionof imidazolidinyl urea as known synthetic preservative also showed antibacterial activities about Staphylococcus aures,Escherichia coli, and Streptococuus mutans. Diameter of zone of inhibition showed 39 mm, 18 mm, and 16 mm,respectively. Water fraction of Artemisia princeps var. orientalis did not show the antibacterial activities aboutStaphylococcus aures, Streptococuus mutans.

Anti-bacterial Effect of Oenothera lamarckiana Aerial Part Extract

Ji Yeong Yang,Pyoengjae Lee,Sa-Hyun Kim 대한의생명과학회 2020 Biomedical Science Letters Vol.26 No.4

Ingestion of food contaminated with microorganism, if not always, could lead to severe health problem. Preservatives has been added to food to prevent food from being contaminated with microorganism. But, these have potential to threaten the health. Therefore, much effort has been taken to find the safe materials showing the anti-microbial activity. In this study, we investigated the anti-bacterial activity of Oenothera lamarckiana aerial part extract against eight bacteria strain. In paper disc assay, extract inhibited the growth of Staphylococcus aureus, Methicillin-resistant S. aureus, Bacillus cereus and Shigella dysenteriae at 200 μg/disc, but not against Escherichia coli, E. coli O157:H7, Salmonella Typhi and S. enteritidis. Minimum inhibitory concentration (MIC) against Staphylococcus aureus, Methicillin-resistant S. aureus, Bacillus cereus and Shigella dysenteriae is 250, 250, 500 and 500 μg/mL, respectively. Compared with reported MIC of other plant resources, O. lamarckiana aerial part extract showed the relatively high anti-bacterial activity. O. lamarckiana aerial part could be suitable for the preservative development. But, it still remains to be studied to evaluate safety and so on.