화학 및 과학 교과서에 기술된 읽기자료 분석 및 활용도 조사

임미경 ( Mi Kyung Lim ),유미현 ( Mi Hyun Yoo ),남석현 ( Seok Hyun Nam ) 경북대학교 과학교육연구소 2012 科學敎育硏究誌 Vol.36 No.1

본 연구의 목적은 과학 교과서에 제시된 읽기자료의 문제점을 파악하기 위해 고등학교 화학Ⅰ 교과서의 읽기자료를 분석하고, 과학 교사의 과학 교과서 읽기자료에 대한 인식을 조사하는 것이다. 이를 위하여제7차 교육과정 및 2009개정 교육과정 고등학교 화학Ⅰ 교과서 각 4종을 택하여 읽기자료의 수, 내용에 따른 제시 유형과 학생의 활동에 따라 비교 분석하였다. 또한 중등 과학교사의 과학 교과서 읽기자료 활용에 대한 인식 조사를 하였다. 연구 결과는 다음과 같다. 첫째, 화학Ⅰ 교과서의 읽기자료를 유형별로 분석한 결과 제7차 화학I 교과서에서는 읽기자료의 비율이 7.9%∼17.1%로 나타났으며, 2009개정 화학I 교과서는 20.6 28.2%비율로 나타났다. 2009개정 교과서의 읽기자료 비율이 증가하였음을 의미한다. 읽기자료 내용별로 분석한 결과 제7차 교육과정에서는``생활 속 과학``이 34.3%로 가장 큰 비율을 차지하였으나, 2009개정에서는``지식의 심화보충``이 23.7%로 가장 높게 나타났다. 학생 활동 유형별로 분석한 결과제7차 교육과정은 읽기자료 13%만이 탐구형 자료인것으로 나타났으나 2009개정에서는 35%가 탐구형자료로 나타나 학생의 탐구활동 향상을 위한 교육과정 목표가 반영되었음을 시사한다. 둘째, 과학 교과서활용 실태에 대한 교사의 인식 조사 결과 67%에 해당하는 교사가 현재 과학 교과서 읽기자료를 수업에 사용하고 있으나 거의 활용하지 않는 교사도 33%나 되었다. 많은 교사들이 실생활과 관련된 통합적 교육을 위해 읽기자료가 필요하다고 응답하였으며``생활 속과학``읽기자료가 교과서에 좀 더 많이 포함되어야 한다고 인식하고 있었다. The purpose of this study was to analyze the reading materials in 7th curriculum and 2009 revised high school chemistryⅠtextbook for identifying the problems of reading material presented in science textbooks and to investigate science teachers` recognition about utilization reading materials in science textbook. For this purpose, each four 7th curriculum and 2009 revised high school chemistryⅠtextbook were analyzed according to the number of reading materials, the type of contents and the type of students` activities. In addition, the secondary school science teachers` recognition about utilization reading materials in science textbook was investigated. The results were as follows: First, anylizing reading materials in chemistryⅠtextbooks showed that and the rate of reading materials were presented from 7.9 to 17.1% in 7th curriculum and from 20.6 to 28.2% in 2009 revised curriculum textbook. It implies that the rate of reading materials in 2009 revised textbooks increases more than those in 7th curriculum textbook. The result of analyzing the type of contents, ``life sciences`` was the largest proportion with 34.3 % in the 7th curriculum chemistry I, but ``enrichment and supplement of knowledge`` was the largest proportion with 23.7% in 2009 revised curriculum. Analyzing the type of student activities, only 13% of the reading materials in 7th National Curriculum textbook was found to be inquiry type, but 35% of the reading materials in the 2009 revised curriculum. appears to be inquiry type. It suggested that the curriculum objectives was reflected in the textbook. Second, investigating recognition of teachers` perceptions of utilization science textbooks, 67% teachers responded that they used the reading materials in their science class, but teachers who didn`t use the reading materials was almost 33%. A large number of teachers responded that the reading materials associated with the real-life needed for integrated education and thought that the reading materials about ``life and science`` should be included in the science textbooks.

7세기 신라 동남해권 성곽의 성격

김현철(Kim, Hyeon-Cheol) 효원사학회 2018 역사와 세계 Vol.- No.53

본고는 7세기를 중심으로 동남해권(거제에서 경주까지의 해안지역)에 분포하는 신라 성곽을 연구대상으로 삼았다. 기존 신라성곽의 연구에서 동남해권 성곽은 주목받지 못하였다. 이와 관련된 기존 연구는 성곽의 성격을 지리적 측면에서 왜구방비와 관련지어 규정하였지만 분명한 근거나 시대적 배경의 제시가 부족하였다. 따라서 정밀조사 및 문헌 자료를 통해 7세기 동남해권 신라성곽의 성격에 대해 살펴보았다. 1장에서는 고고학 자료를 활용하여, 군현방어 및 해로의 감시를 위해 군현 배후산지에 중형급성곽(700m~1200m), 해안 및 島嶼에 소형급성곽(600m이하)이 분포함을 제시하였다. 성곽의 중심 시기는 7세기이며, 7세기 전반까지는 중형급성곽을 축조하여 해안과 인접한 군현으로 방어체계를 확장하였고, 7세기 후반에는 소형급성곽으로 감시체계를 보완한 변화양상을 검토하였다. 2장에서는 동남해권 성곽운용의 시대적 배경에 대해 7세기 전반에는 왜의 신라침공의도와 대왜적대감의 발생으로 제시하고, 7세기 후반에는 왜의 신라2성 공격과 전후 당과 왜의 접촉으로 인해 대왜적대감이 지속되었지만, 신라의 재정상태 악화와 축성의 부정적 인식으로 소형급성곽이 축조되었다고 보았다. 따라서 7세기 신라 동남해권 성곽의 성격은 대왜적대감을 바탕으로 국내외사정을 고려하여, 7세기부터 체계적으로 대왜방비만을 위해 마련된‘ 대왜맞춤형성곽’으로 제시하였다. This study focuses fortresses of Silla(新羅) distributed over region of East-South sea(the coastal area from Geoje to Gyeongju) around the 7<SUP>th</SUP> century. They have not noticed in study for fortress of Silla. Even if previous studies have defined characteristic of fortress relate to defense against Japanese raiders geographically, there are lacks of apparent reasons or arguments about historical backdrop. Therefore, this study analyzed the characteristic of the fortresses distributed over region of East-South sea around the 7th century based on the results of archaeological survey and literature materials. In the first chapter, it was suggested that the midium-fortress (700m~1200m) was spread in mountain area from the behind Gunhyeon (郡縣) and the small-fortress(less than 600m) was in the coastal and insular areas for watching seaway and defending Gunhyeon. Their aspect of transition is considered that Silla had extended the defense system focusing on Gunhyeon near coast through constructing the former until early 7<SUP>t</SUP>h century and complemented it with the latter. Second chapter explains the historical backdrop about operating of fortresses over region of East-South sea. In early 7<SUP>th</SUP> century, there are Japanese intention the invasion of Silla and hospitality between them. In the late of 7<SUP>th</SUP> century, despite the lasting hospitality toward Japanese after Japanese attack to second castle of Silla and her contact with Tang, Silla constructed the small-fortress because Silla have the poor finance and negative awareness on castellation. In conclusion, Considering domestic and foreign affairs of Silla on the basis of her hospitality toward Japanese, the characteristic of the fortresses distributed over region of East-South sea around the 7<SUP>th</SUP> century seems ‘defensive fortress against japan(倭)’, which were created in order to defend only against Japan systematically from the 7<SUP>th</SUP> century.

Let-7c Inhibits NSCLC Cell Proliferation by Targeting HOXA1

Zhan, Min,Qu, Qiang,Wang, Guo,Liu, Ying-Zi,Tan, Sheng-Lan,Lou, Xiao-Ya,Yu, Jing,Zhou, Hong-Hao Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1

Objective: The aim of the present study was to explore mechanisms by which let-7c suppresses NSCLC cell proliferation. Methods: The expression level of let-7c was quantified by qRT-PCR. A549 and H1299 cells were transfected with let-7c mimics to restore the expression of let-7c. The effects of let-7c were then assessed by cell proliferation, colony formation and cell cycle assay. Mouse experiments were used to confirm the effect of let-7c on tumorigenicity in vivo. Luciferase reporter assays and Western blotting were performed to identify target genes for let-7c. Results: HOXA1 was identified as a novel target of let-7c. MTS, colony formation and flow cytometry assays demonstrated that forced expression of let-7c inhibited NSCLC cell proliferation by inducing G1 arrest in vitro, consistent with inhibitory effects induced by knockdown of HOXA1. Mouse experiments demonstrated that let-7c expression suppressed tumorigenesis. Furthermore, we found that let-7c could regulate the expression of HOXA1 downstream effectors CCND1, CDC25A and CDK2. Conclusions: Collectively, these results demonstrate let-7c inhibits NSCLC cell proliferation and tumorigenesis by partial direct targeting of the HOXA1 pathway, which suggests that restoration of let-7c expression may thus offer a potential therapeutic intervention strategy for NSCLC.

소년의료보호시설(소년법 제7호처분)제도에 대한 독창적 개선방안

천정환 한국교정복지학회 2016 교정복지연구 Vol.- No.40

소년의료보호시설 등이란 보호처분의 일종인 7호처분이 부과된 보호소년에 대한 품행교정과 환경조정, 의료라는 의료보호공공재의 생산을 하는 의료보호기관 등을 말한다. 소년범죄에 대한 보호처분의 일종인 7호처분제도는 소년보호의 가외성, 특수교육이념, 특별예방이념, 사회복지, 가족치료 등을 이념으로 한다. 이러한 7호처분의 집행은 법무부의 지휘와 감독을 받는 관료적인 대전의료소년원과 가정법원의 지휘와 감독을 받는 비관료적인 일반병원(위탁병원 등)으로 구분되어 시행이 된다. 그런데 7호처분의 집행은 단순히 형사정책적 접근으로 되어서는 안되는 바 그 이유는 7호처분의 특성상 아동발달이론, 심리학, 발달론, 교육학, 인지과학, 교정급식학, 교정학 등으로 구성되어 있기 때문이다. 따라서 본 논문에서는 7호처분 관련 법령과 운영실태를 소개하고 내용과 선행연구들을 독창적 관점에서 비판하였다. Juvenile offender medical protection agency which is introduced in the new revised juvenile act(2007) is medical rehabilitation agency which is based on special criminology policy which produces medical protection public service such on the adjustment of environment of juvenile offender, medical treatment and correction treatment, The ideology of 7treatment in the article 32 of current juvenile Law by juvenile court is composed of the redundancy of youth medical education in the youth medical detention center which confines juvenile offenders who is sentenced 7treatment of current juvenile Law who suffer from physical disease, physical disease, drug abuse, mental retardation, mental disease and specific education ideology and specific education ideology and specific prevention ideology and social welfare, family therapy. 7 treatment execution agency is invided into Dae Jun youth medical detention center which is directed by Justice Department and genenal hospital and so on which is directed by juvenile courts. The study of the 7treatment should not be approached in the simple criminology policy due to the its interdisciplinary character. The study of the 7treatment in the article 32 of current juvenile Law should be approached in the interdisciplinary view such on psychology, development theory, science education cognitive science, meals science correction and medical. Therefore, in this article, I would like to introduce the system of 7treatment in the article 32 of current juvenile Law and to assert improvement device of the youth medical protection agency in the current juvenile Law which the existing studies have ignored about its problems throughout the interdisciplinary view and my original view.

7세기 신라 동남해권 성곽의 성격

김현철 효원사학회 2018 역사와 세계 Vol.- No.53

This study focuses fortresses of Silla(新羅) distributed over region of East-South sea(the coastal area from Geoje to Gyeongju) around the 7th century. They have not noticed in study for fortress of Silla. Even if previous studies have defined characteristic of fortress relate to defense against Japanese raiders geographically, there are lacks of apparent reasons or arguments about historical backdrop. Therefore, this study analyzed the characteristic of the fortresses distributed over region of East-South sea around the 7th century based on the results of archaeological survey and literature materials. In the first chapter, it was suggested that the midium-fortress (700m~1200m) was spread in mountain area from the behind Gunhyeon(郡縣) and the small-fortress(less than 600m) was in the coastal and insular areas for watching seaway and defending Gunhyeon. Their aspect of transition is considered that Silla had extended the defense system focusing on Gunhyeon near coast through constructing the former until early 7th century and complemented it with the latter. Second chapter explains the historical backdrop about operating of fortresses over region of East-South sea. In early 7th century, there are Japanese intention the invasion of Silla and hospitality between them. In the late of 7th century, despite the lasting hospitality toward Japanese after Japanese attack to second castle of Silla and her contact with Tang, Silla constructed the small-fortress because Silla have the poor finance and negative awareness on castellation. In conclusion, Considering domestic and foreign affairs of Silla on the basis of her hospitality toward Japanese, the characteristic of the fortresses distributed over region of East-South sea around the 7th century seems ‘defensive fortress against japan(倭)’, which were created in order to defend only against Japan systematically from the 7th century. 본고는 7세기를 중심으로 동남해권(거제에서 경주까지의 해안지역)에 분포하는 신라 성곽을 연구대상으로 삼았다. 기존 신라성곽의 연구에서 동남해권 성곽은 주목받지 못하였다. 이와 관련된 기존 연구는 성곽의 성격을 지리적 측면에서 왜구방비와 관련지어 규정하였지만 분명한 근거나 시대적 배경의 제시가 부족하였다. 따라서 정밀조사 및 문헌 자료를 통해 7세기 동남해권 신라성곽의 성격에 대해 살펴보았다. 1장에서는 고고학 자료를 활용하여, 군현방어 및 해로의 감시를 위해 군현배후산지에 중형급성곽(700m~1200m), 해안 및 島嶼에 소형급성곽(600m이하)이 분포함을 제시하였다. 성곽의 중심 시기는 7세기이며, 7세기 전반까지는 중형급성곽을 축조하여 해안과 인접한 군현으로 방어체계를 확장하였고, 7세기 후반에는 소형급성곽으로 감시체계를 보완한 변화양상을 검토하였다. 2장에서는 동남해권 성곽운용의 시대적 배경에 대해 7세기 전반에는 왜의 신라침공의도와 대왜적대감의 발생으로 제시하고, 7세기 후반에는 왜의 신라2성 공격과 전후 당과 왜의 접촉으로 인해 대왜적대감이 지속되었지만, 신라의 재정상태 악화와 축성의 부정적 인식으로 소형급성곽이 축조되었다고 보았다. 따라서 7세기 신라 동남해권 성곽의 성격은 대왜적대감을 바탕으로 국내외 사정을 고려하여, 7세기부터 체계적으로 대왜방비만을 위해 마련된 ‘대왜맞춤형성곽’으로 제시하였다.

7.7선언체제의 평가와 대안체제의 모색

박찬봉(Chanbong Park) 한국정치학회 2008 한국정치학회보 Vol.42 No.4

1988.7.7 노태우대통령이 출범시킨 7.7선언체제는 남북간의 화해 협력을 통해 신뢰를 쌓음으로써 제도적인 통일을 이루고자 하였다. 이론적으로는 유럽통합 이론인 기능주의가 중심이 되었다. 그러나 지난 20년간의 남북관계는 기능주의의 핵심주장인 파급효과가 제대로 작동되지 않았음을 보여 주었다. 특히 막대한 재원을 투입하여 추진된 햇볕정책도 이러한 흐름을 바꾸지 못하였다. 따라서 새로운 패러다임 전환이 요구된다. 통일을‘국민국가형성의 완성’이라는 목표로 정의하고 북한의 개혁과 개방을 핵심 관건이라고 볼 때 그 이론체계는 이를 가장 잘 설명할 수 있는 제도주의에 의해 재정립 되어야 한다. 당면하여서는 통일의 과도적 단계와 북한의 개혁 과정을 관리하기 위한 제도로서 남북연합을 모색할 필요가 있다. 이명박정부의 비핵.개방.3000구상(상생 공영 정책)은 제도주의와 남북연합의 틀 속에서 더 잘 설명될 수 있다. The ‘July 7th Declaration Paradigm’ launched in 1988 by the President Roh Tae Woo aimed at achieving Korean unification by building confidence through inter Korean reconciliation and cooperation. However, after 20 years, it is evident that the paradigm’s key element of spill over effects, which derives from neofunctionalism, has not operated properly. The Sunshine policy of the past ten years further vindicated the failure of the functionalism. This calls for a paradigm shift. Considering that Korean unification constitutes the Korean nation building and that North Korean reform and opening is indispensable in the process, I propose that this new approach can be better explained by institutionalism. In that context, I also claim that inter Korean confederation needs to be considered as an interim institutional stage for Korean unification. President Lee Myong Bak’s policy of denuclearization, opening, and 3000, aka Vision 3000, can be better explained in relation with institutionalism and inter Korean confederation.

7a-Hydroxycholesterol Elicits TLR6-Mediated Expression of IL-23 in Monocytic Cells

( Hyun Chul Seo ),( Sun Mi Kim ),( Seong Kug Eo ),( Byung Yong Rhim ),( Koanhoi Kim ) 한국응용약물학회 2015 Biomolecules & Therapeutics(구 응용약물학회지) Vol.23 No.1

We investigated the question of whether 7-oxygenated cholesterol derivatives could affect inflammatory and/or immune responses in atherosclerosis by examining their effects on expression of IL-23 in monocytic cells. 7α-Hydroxycholesterol (7αOHChol) induced transcription of the TLR6 gene and elevated the level of cell surface TLR6 protein in THP-1 monocytic cells. Addition of an agonist of TLR6, FSL-1, to TLR6-expressing cells by treatment with 7αOHChol resulted in enhanced production of IL-23 and transcription of genes encoding the IL-23 subunit α (p19) and the IL-12 subunit β (p40). However, treatment with 7-ketocholesterol (7K) and 7β-hydroxycholesterol (7βOHChol) did not affect TLR6 expression, and addition of FSL-1 to cells treated with either 7K or 7βOHChol did not influence transcription of the genes. Pharmacological inhibition of ERK, Akt, or PI3K resulted in attenuated transcription of TLR6 induced by 7αOHChol as well as secretion of IL-23 enhanced by 7αOHChol plus FSL-1. Inhibition of p38 MAPK or JNK resulted in attenuated secretion of IL-23. These results indicate that a certain type of 7-oxygenated cholesterol like 7αOHChol can elicit TLR6-mediated expression of IL-23 by monocytic cells via PI3K/Akt and MAPKs pathways.

FBXW7-mediated stability regulation of signal transducer and activator of transcription 2 in melanoma formation

Lee, Cheol-Jung,An, Hyun-Jung,Kim, Seung-Min,Yoo, Sun-Mi,Park, Juhee,Lee, Ga-Eun,Kim, Woo-Young,Kim, Dae Joon,Kang, Han Chang,Lee, Joo Young,Lee, Hye Suk,Cho, Sung-Jun,Cho, Yong-Yeon National Academy of Sciences 2020 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.117 No.1

<P><B>Significance</B></P><P>The physiological relevance of STAT2 (a member of STAT family) in melanoma formation is clearly shown using a human skin tissue array. Moreover, FBXW7-mediated STAT2 protein stability regulation via ubiquitination is shown to play an essential role in melanoma cell proliferation in monolayer and anchorage-independent 3D culture systems. The molecular mechanisms that regulate STAT2 protein stability by FBXW7 include the interaction between CCD and DBD domains of STAT2 and the WD40 domain of FBXW7. STAT2 phosphorylation at the putative degron motifs that contain Ser381, Thr385, and Ser393 might be mediated by GSK3β. These serve as critical amino acids that form hydrogen bonds with the WD40 domain of FBXW7. Thus, the FBXW7–STAT2 signaling axis is an important target for melanoma treatment.</P><P>In this study, we provide critical evidence that STAT2 stability regulation plays an essential role in melanoma cell proliferation and colony growth. We found that the interaction of FBXW7 and STAT2 induced STAT2 destabilization via a ubiquitination-mediated proteasomal degradation pathway. Notably, GSK3β-mediated STAT2 phosphorylation facilitated STAT2–FBXW7 interactions via the DNA binding domain of STAT2 and domains 1, 2, 6, and 7 of FBXW7 WD40. Importantly, the inverse correlation between protein levels of STAT2 and FBXW7 were observed not only in human melanoma cells but also in a human skin cancer tissue array. The relationship between protein levels of STAT2 and FBXW7, cell proliferation, and colony growth were similarly observed in the melanoma cell lines SK-MEL-2, -5, and -28. Moreover, STAT2 knockdown in melanoma cells suppressed melanoma cell proliferation and colony formation. These data demonstrated that FBXW7-mediated STAT2 stability regulation plays an essential role in melanoma cell proliferation and cancer growth.</P>

7호 처분 집행의 법적 근거 명확화에 관한 연구

박찬걸 ( Park Chan-keol ) 한국소년정책학회 2014 少年保護硏究 Vol.25 No.-

According to the subparagraph 7, paragraph 1, article 32 of the current Juvenile Act, the judge of Juvenile Court can rule a disposition of 'entrusting to juvenile medical care facilities according to the law on treatment of hospital, sanitarium or protected juvenile' among the types of protective dispositions based on the hearing result. In this context, an issue is raised on whether ‘juvenile medical care facilities’ being referred to in subparagraph 7 disposition among the protective disposition of the Juvenile Act can be identified with medical & rehabilitation education juvenile hall' being referred to in the protective disposition ordinances. If it can be identified, an issue is raised on whether the disposition of subparagraph 7 also conform to 'the disposition of sending to juvenile hall'. It is because it could cause the result of subparagraph 7 also conforming to the disposition of sending to juvenile hall if juvenile medical care facilities are medical & rehabilitation education juvenile hall since in the existing discussion, the disposition of sending to juvenile hall commonly refers only to subparagraph 8 or 10 disposition among protective dispositions. In terms of the opposite case, if 'juvenile medical care facilities' being referred to in subparagraph 7 among protective dispositions in the Juvenile Act cannot be identified with 'medical & rehabilitation education juvenile hall' being referred to in protective disposition ordinances, it can be said that there is a gap in legislation only with existing ordinances due to lack of grounds to enforce subparagraph 7 at medical juvenile hall among the protective dispositions of paragraph 1, article 32 of the Juvenile Act. For such reason, it would be necessary to newly establish regulations related to the concept of 'juvenile medical care facilities' specified in the Juvenile Act, grounds in ordinances and grounds & principles of medical treatment. Hereinafter, the significance and contents of subparagraph 7 will be reviewed focusing on the necessity of subparagraph 7 disposition and process of change of organization in charge, period of subparagraph 7 disposition and status of recent dispositions, the cost burden issue of 'entrusted disposition such as hospital', status of transferring the subjects of subparagraph 9 & 10 dispositions to the subparagraph 7 disposition organization. In addition, it will examine existing discussions on re-establishment of the practical meaning of the entrusted disposition to juvenile medical care facilities and the concept of protected juvenile, as well as examine the status of legislative improvement. It will conclude the discussion by analyzing future assignments to supplement the task of clarifying legal grounds to enforce subparagraph 7 disposition.

박테리오파아지 T7 의 기능에 관한 연구;복제단백질간의 단백질 상호작용

김학준,김영태 한국생명과학회 1996 생명과학회지 Vol.6 No.3

박테리오파지 T7 gene 2.5 단백질은 single-stranded DNA 결합 단백질로 박태리오파지 T7의 DNA복제, 재조합, 및 수선에 필수적으로 요구된다. Gene 2.5 protein은 T7의 DNA 합성과 성장에 필수적인 단백질이다. Gene 2.5 Protein이 중요시 되는 이유는 이 단백질이 T7의 다른 복제 필수단백질인 T7의 다른 복제 필수단백질인 T7 DNA polymerase 와 gene 4 protein(helicase/primase)와 서로 상호작용할 것으로 제안되었기 때문이다. (Kim and Richardson, J. Biol. Chem., 1992;1994). 이 단백질의 단백질 상호작용을 가능하게 하는 domain은 carboxyl-terminal domain일 것으로 여러 실험에서 대두되었기에, 이 domain의 특성을 파악하기 위해 야생형과 변이체 gene 2.5 단백질들을 각각 GST에 융합한후 fusion 단백질을 정제하였다. 정제된 이 융합 단백질들의 carboxyl-terminal domain이 T7 복제 단백질들과 상호작용을 조사하는지를 조사하기 위해 affinity chromatography로 이용하였다. 실험 결과, 아생형 GST-gene 2.5 융합단잭질(GST-2.5 (WT))는 T7 DNA polymerase 와 상호작용을 하였지만. 변이형 융합단백질(GST-2.5$\Delta$21C)는 interaction을 하지 못했다. 이 결과는 carbohyl-terminal domain이 단백질-단백질 상호작용을 하는데 직접적으로 관여하는 것을 증명하였다. 또한,GST2.5(WT)는 gene 4 protein(helicase/primase)와 직접 상호작용을 하나. GST2.5$\Delta$21C는 상호작용을 하지 못하는 것으로 나타났다. 따라서 gene 4 proteins와의 상호작용에도 gene 2.5 protein의 carboxyl-terminal domain이 직접 관여 한다는 것이 증명되었다. 이상의 결과에서 gene 2.5 protein은 박테리오파지 T7 의 유전자 목제 시 단백질-단백질 상호작용에 관혀아며, 특히 gene 2.5 protein의 carboxyl-terminal domain이 이러한 상호작용에 직접적으로 관여하는 domain이라는 것을 알 수가 있었다. Bacteriophage T7 gene 2.5 protein, a single-stranded DNA binding protein, is required for T7 DNA replication, recombination, and repair. T7 gene 2.5 protein has two distinctive domains, DNA binding and C-terminal domain, directly involved in protein-protein interaction. Gene 2.5 protein participates in the DNA replication of Bacteriophage T7, which makes this protein essential for the T7 growth and DNA replication. What gene 2.5 protein makes important at T7 growth and DNA replication is its binding affinity to single-stranded DNA and the protein-protein important at T7 DNA replication proteins which are essential for the T7 DNA synthesis. We have constructed pGST2.5(WT) encoding the wild-type gene 2.5 protein and pGST2.5$\Delta $21C lacking C-terminal 21 amino acid residues. The purified GST-fusion proteins, GST2.5(WT) and GST2.5(WT)$\Delta$21C, were used for whether the carboxyl-terminal domain participates in the protein-protein interactions or not. GST2.5(WT) and GST2.5$\Delta$21C showed the difference in the protein-protein interaction. GST2.5(WT) interacted with T7 DNA polymerase and gene 4 protein, but GST2.5$\Delta$21C did not interact with either protein. Secondly, GST2.5(WT) interacts with gene 4 proteins (helicase/primase) but not GST2.5$\Delta$21C. these results proved the involvement of the carboxyl-terminal domain of gene 2.5 protein in the protein-protein interaction. We clearly conclude that carboxy-terminal domain of gene 2.5 protein is firmly involved in protein-protein interactions in T7 replication proteins.