Increased Serine Phosphorylation and Activation of STAT1 by Oncogenic Ras Transfection

Ji-Hyun Song,Eui-Young So,Choong-Eun Lee 한국분자세포생물학회 2002 Molecules and cells Vol.13 No.2

Interferon (IFN)-γ induces the activation of a signal transducer and activator of transcription 1 (STAT1), and regulates the growth response of diverse cell types. Although STAT1 activation is primarily induced upon tyrosine phosphorylation by Jak1 and Jak2 (the IFN-γ receptor-associated tyrosine kinases), the full activation of STAT1 is thought to involve serine phosphorylation by unidentified protein kinases. As a part of our on-going investigation on the deregulation of STATs in oncogene-transformed cells, we found that STAT1 activation was efficiently induced by IFN-γ in oncogenic Ras-transformed fibroblasts, compared to the parental fibroblasts. This was shown by target DNA binding activity, nuclear translocation, and tyrosine phosphorylation of STAT1. Using a transient transfection system, we directly demonstrated that Ras-transfection up-regulated the IFN-γ-induced STAT1 activation with a concomitant increase in Erk MAPK activity. Notably, the enhanced serine phosphorylation of STAT1 was observed upon Ras-transfection, which was specifically associated with the induction of MAPK, but not Akt activity in these cells. The data suggest that Ras/ MAPK module components may positively regulate STAT1 activity by inducing the serine-phosphorylation of STAT1. This would contribute to the enhanced tyrosine phosphorylation, nuclear translocation, and DNA-binding of STAT1 upon exposure of the cells to IFN-γ in the Ras-transformed cells.

자성나노입자 주입에 의한 혈청의 인터페론-감마 변화 특성

이한승,전찬호,HASAN MAHBUB,최종구,AKTERHAFEZA,배예빈,이상석 한국물리학회 2022 새물리 Vol.72 No.9

Magnetic nanoparticles are widely used for treatment and diagnosis in the medical field. Given that their position is easily manipulated using a magnetic field, they can be developed as targeted immunotherapeutic agents. This study aims to determine whether dextran coat on 40 nm Fe3O4 magnetic nanoparticles is medically toxic. The interferon-gamma (IFN-γ) concentration is measured using a sandwich ELISA method. IFN-γ is a potent inflammatory cytokine that increases with toxic immune responses. If magnetic nanoparticles are toxic, then an inflammatory reaction occurs when they are injected into the body through the tail vein of a mouse, resulting in a change in IFN-γ concentration in blood serum. However, when dextran-coated magnetic nanoparticles are injected in vivo, the IFN-γ concentration in the mouse serum does not increase, so an inflammatory reaction does not occur. Therefore, the dextran-coated magnetic nanoparticles are biocompatible and suitable for antibody-conjugated targeted immunotherapeutic drug development. 치료와 진단의 목적으로 의료분야에 많이 사용되고 있는 자성나노입자는 자기장에 의해 위치조작이 가능한 장점이 있어 표적 면역 치료제로 개발될 수 있다. 40 nm 크기의 자철석 Fe3O4 에 덱스트란이 코팅된 자성나노입자를 의학적으로 독성 여부를 확인하기 위해 샌드위치 ELISA 방법을 이용하여 인터페론-감마 (IFN-γ)의 농도를 측정하게 된다. 자성나노입자가 독성이 있다면 마우스 꼬리 정맥을 통해 체내 주입되었을 때 염증 반응이 일어나 혈액의 혈청 내 IFN-γ 농도의 변화를 나타내게 된다. 면역 반응이 일어나면 사이토카인이 분비되기 때문에 IFN-γ가 증가하게 된다. 덱스트란-자성나노입자를 생체 내에 주입하였을 때 마우스의 혈청 내의 IFN-γ가 증가하지 않았기에 염증 반응이 일어나지 않아서 독성이 없는 생체적합성 물질로 면역치료에 적합한 단일클론항체를 결합한 자성나노입자를 의약학적으로 사용이 가능할 수 있다.

Application of genetically engineered Salmonella typhimurium for interferon-gamma-induced therapy against melanoma

Yoon, W.,Park, Y.C.,Kim, J.,Chae, Y.S.,Byeon, J.H.,Min, S.H.,Park, S.,Yoo, Y.,Park, Y.K.,Kim, B.M. Pergamon Press 2017 European journal of cancer Vol.70 No.-

Salmonella have been experimentally used as anti-cancer agents, because they show selective growth in tumours. In this study, we genetically modified attenuated Salmonella typhimurium to express and secrete interferon-gamma (IFN-γ) as a tumouricidal agent to enhance the therapeutic efficacy of Salmonella. IFN-γ was fused to the N-terminal region (residues 1-160) of SipB (SipB160) for secretion from bacterial cells. Attenuated S. typhimurium expressing recombinant IFN-γ (S. typhimurium (IFN-γ)) invaded the melanoma cells and induced cytotoxicity. Subcutaneous administration of S. typhimurium (IFN-γ) also efficiently inhibited tumour growth and prolonged the survival of C57BL/6 mice bearing B16F10 melanoma compared with administration of phosphate-buffered saline (PBS), unmodified S. typhimurium or S. typhimurium expressing empty vector (S. typhimurium [Vec]) in a natural killer (NK) cell-dependent manner. Moreover, genetically modified Salmonella, including S. typhimurium (IFN-γ), showed little toxicity to normal tissues with no observable adverse effects. However, S. typhimurium (IFN-γ)-mediated tumour suppression was attributed to direct killing of tumour cells rather than to stable anti-tumour immunity. Collectively, these results suggest that tumour-targeted therapy using S. typhimurium (IFN-γ) has potential for melanoma treatment.

Liquid crystal-based aptasensor for the detection of interferon-γ and its application in the diagnosis of tuberculosis using human blood

Kim, Hyeong Jin,Jang, Chang-Hyun Elsevier 2019 Sensors and actuators. B Chemical Vol.282 No.-

<P><B>Abstract</B></P> <P>We report the development of a liquid crystal (LC)-based aptasensor for the detection of interferon-γ (IFN-γ) in human blood as well as the diagnosis of tuberculosis (TB). In this system, the binding of IFN-γ to an aptamer immobilized on a surface disrupts the orientation of LCs, inducing a transition from a homeotropic orientation to a random one. This change in the orientation of the LCs can easily be converted and observed as a shift from a dark optical LC image to a bright one under a polarized light microscope. Through this sensing mechanism, IFN-γ levels as low as 1 pM (17 pg/ml) could be detected. This LC-based aptasensor was employed for the diagnosis of TB using blood samples from patients with latent TB. With this LC-based approach, not only could IFN-γ readily be detected, but also latent TB could be diagnosed using human blood simply and effectively without any intricate processes or instrumentation. Therefore, our present research provides a promising IFN-γ sensor with applications in clinical diagnosis of various infectious diseases, and in immunological research.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Detection of IFN-γ using LC-based aptasensor. </LI> <LI> The binding of IFN-γ to an aptamer induced an orientational transition of LCs. </LI> <LI> This LC-based aptasensor can be employed for diagnosis of latent TB in human blood. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Cytokine Pattern is Affected by Training Intensity in Women Futsal Players

Abdossaleh Zar,Fatemeh Ahmadi,Maryamosadat Miri,Hassan Ali Abedi,Mohsen Salesi 대한면역학회 2016 Immune Network Vol.16 No.2

To find the relation between exercise and cytokines, we examined the effect of the training intensity on the levels of cytokines, including interferon-gamma (IFN-γ), interlukine- 4 (IL-4) and interlukine-4/interferon-gamma ratio (IL-4/IFN-γ ratio) in female Futsal players. Twelve well-trained female college Futsal players aged 19∼22 participated in this study. The athletes completed 30-min of running at 60∼65% maximal heart rate [moderate-intensity exercise], and 30-min of running at 75∼80% maximal heart rate [high-intensity exercise]. peripheral blood samples were collected 24 h before and 24 h and 48 h after each of the exercise bouts. finding showed that The 30-min bout of moderate-intensity exercise induced a significant increase in IFN-γ (p=0.01) and significant decreases in IL-4 (p=0.001) and IL-4/IFN-γ ratio (p=0.003). And also, 30-min of running at 75∼80% maximal heart rate induced increase in IFN-γ (p=0.07) and decreased in IL-4 (p=0.01) and IL-4/IFN-γ ratio (p=0.06) that these changes not significantly. In summary, exercise intensity can effect on the magnitude of changes in cytokines. It seems that moderate intensity exercise enhances cytokine pattern in female college Futsal players.

Increased Serine Phosphorylation and Activation of STAT1 by Oncogenic Ras Teansfextion

Song, Ji-Hyun,So, Eui-Young,Lee, Choong-Eun Korean Society of Molecular Biology 2002 Molecules and cells Vol.13 No.2

Recombinant interferon-γ activates immune responses against Edwardsiella tarda infection in the olive flounder, Paralichthys olivaceus

Jung, C.Y.,Hikima, J.i.,Ohtani, M.,Jang, H.B.,del Castillo, C.S.,Nho, S.W.,Cha, I.S.,Park, S.B.,Aoki, T.,Jung, T.S. Academic Press 2012 FISH AND SHELLFISH IMMUNOLOGY Vol.33 No.2

Interferon gamma (IFN-γ) is a cytokine that plays a very important role in defining Th1 immune response in all vertebrates. In this study, recombinant IFN-γ (rIFN-γ) from the olive flounder (Paralichthys olivaceus) was produced in an Escherichia coli system using a pET expression vector. Stimulation of whole kidney leukocytes (immune-related cells) in vitro with the resulting rIFN-γ significantly induced the gene expression of interleukin-1β (IL-1β), signal transducer and activator of transcription 1 (STAT1), CXCL13-like chemokine (CXCL13), and IFN-γ. rIFN-γ also weakly induced the expression of IL-1β, tumor necrosis factor-α (TNF-α), CXCL13, and IFN-γ in olive flounder-derived HINAE (non-immune) cells. The effects of rIFN-γ against Edwardsiella tarda infection in vivo were assessed by intraperitoneally injecting a mixture of rIFN-γ (100 ng) and E. tarda (1 x 10<SUP>5</SUP> CFU/ml) into the olive flounder. The survival rate in the rIFN-γ-injected group was 60% compared to 0% in the group treated with E. tarda only, demonstrating that olive flounder IFN-γ is effective in reinforcing immune responses and preventing against edwardsiellosis.

EFFECT OF THE METHANOL EXTRACT OF KOREAN TRADITIONAL MEDICINE HERB OLDENLANDIAE DIFFUSAE HERBA ON PROLIFERATION AND DIFFERTATION OF CANCER CELL

Jeon,Byung-Hun,Moon,Gu,Woo,Won-Hong 대한동의병리학회 1998 동의생리병리학회지 Vol.12 No.2

백화사설초는 여러 가지 암세포의 성장을 억제할 수 있는 항암효과가 뛰어난 약재로, 근래에 많이 암환자에 활용되어지고 있으며, 실제 실험적으로도 그 효과가 입증되어지고 있다. 본 연구에서는 백화사설초가 사람 암세포의 증식과 분화에 어떠한 효과를 미치는지를 알아보기 위하여 monocytic leukemic cell line인 U937을 백화사설초 추출물만을 처리하고, 또한 백화사설초(10 ㎍/ml)의 농도에서 활성화된 단핵세포(ODH-MNC-CM)를 사용하였다. 그 결과 백화사설초 단독으로 사용할 경우 암세포의 분화를 유도하는 작용은 가지고 있지 않았지만 백화사설초가 Leukemic U937 세포에 대한 증식억제와 분화를 유도하는 IFN-γ, TNF-α와 같은 cytokine의 생산을 야기할 수 있다는 것을 알았으며, 백화사설초 추출물의 항종양효과와 암세포분화효과는 이러한 cytokines의 생성과 관련된 것으로 생각된다. Oldenlandiae diffusae Herba is herb medicine with antitumor activity capable of suppressing the growth of some cancer cell lines. In the present study, we have isolated a methanol extraction of Oldenlandiae diffusae Herba(ODH) and investigated its effect on the proliferation and differentiation of human cancer cell line using an in vitro culture system. Our results showed that the conditioned medium from ODH(10 ㎍/ml)-stimulated blood mononuclear cells (ODH-MNC-CM) treatment induced about 50% of the cells differentiating into mature monocytes/macrophages of CD 11b, CD 14, and CD68. Yet, the differentiated U937 cells also had functions of phagocytosis and superoxide production. However, ODH alone or normal MNC-CM had no such effects. The levels of interferon (IFN)-γ, tumor necrosis factor (TNF)-α, and interleukin (IL)-1 were very low in normal MNC-CM, and they were greatly increased in MNC-CM prepared with ODH stimulation. Antibody neutralization studies further revealed that the tumoricidal and differentiating effects of ODH-MNC-CM were mainly derived from the elevated cytokines, especially IFN-γ and TNF-α. These two cytokines acted synergistically on inhibiting cell growth and inducing differentiation of the target U937 cells.

12-o-Tetradecanoyl Phorbol 13-Acetate 및 Phytohemagglutinin으로 자극한 사람 말초혈액 단핵세포에 의한 인터류킨-2 및 인터류킨-6의 생성

이수영,임주원,강정희 大韓血液學會 1996 大韓血液學會誌 Vol.31 No.3

Gr-1^intCD11b⁺ myeloid-derived suppressor cells accumulate in corneal allograft and improve corneal allograft survival

Choi, Wungrak,Ji, Yong Woo,Ham, Hwa-Yong,Yeo, Areum,Noh, Hyemi,Jin, Su-Eon,Song, Jong Suk,Kim, Hyeon Chang,Kim, Eung Kwon,Lee, Hyung Keun Federation of American Societies for Experimental 2016 Journal of Leukocyte Biology Vol.100 No.6

<P>We identified the characteristics of myeloid-derived suppressor cells (MDSCs) and investigated their mechanism of induction and their functional role in allograft rejection using a murine corneal allograft model. In mice, MDSCs coexpress CD11b and myeloid differentiation antigen Gr-1.Gr-1(+)CD11b(+) cells infiltrated allografted corneas between 4 d and 4 wk after surgery; however, the frequencies of Gr-1(+)CD11b(+) cells were not different between accepted and rejected allografts or in peripheral blood or BM. Of interest, Gr-1(int)CD11b(+) cells, but not Gr-1(hi)CD11b(+) cells, infiltrated the accepted graft early after surgery and expressed high levels of immunosuppressive cytokines, including IL-10, TGF-beta, and TNF-related apoptosis-inducing ligand. This population remained until 4 wk after surgery. In vitro, only high dose (>100 ng/ml) of IFN-gamma plus GM-CSF could induce immunosuppressive cytokine expression in Gr-1(int)CD11b(+) cells. Furthermore, adoptive transfer of Gr-1(int)CD11b(+) cells reduced T cell infiltration, which improved graft survival. In conclusion, high-dose IFN-gamma in allograft areas is essential for development of Gr-1(int)CD11b(+) MDSCs in corneal allografts, and subtle environmental changes in the early period of the allograft can result in a large difference in graft survival.</P>