[PB-0094] Genome-Wide Association Study of Leaf Chlorophyll Content Using High-Density 50K SNP Array in Peanut Accessions

Kunyan Zou(Kunyan Zou),Nayoung Kwon(Nayoung Kwon),Tae-Hwan Jun(Tae-Hwan Jun) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

[PB-0095] Genome-Wide Association Study and Transcriptome Analysis of Salinity Tolerance in Peanuts(Arachis hypogaea L.)

Kunyan Zou(Kunyan Zou),Seung-Ah Han(Seung-Ah Han),Min-jae Choi(Min-jae Choi),Min-jeong Lee(Min-jeong Lee),Da-Hye Jeon(Da-Hye Jeon),Tae-Hwan Jun(Tae-Hwan Jun) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

Dynamic response characteristics of an innovative turretless low motion FPSO hull in central GoM ultra-deep waters

Zou, Jun Techno-Press 2022 Ocean systems engineering Vol.12 No.2

In oil and gas industry, FPSO concept is the most popular hull form and ship shaped hull form dominants the FPSO market. Only a non-ship-shaped hull in operations with minor market shares is the cylindrical FPSO hull with medium to small storage capability. To add contracting options and competitions to reduce field development costs, an innovative turretless low motion hull, eco-FPSO, with 1MM bbls oil storage capacity and suitable for installing topsides modulars and equipping with regular SCRs, was first introduced in Zou (2020a). Dynamic characteristic responses of the eco-FPSO compared to the traditional SS-FPSO hull and DD-Semi platform are presented and discussed in this paper, suitability and feasibility of the proposed hull have been demonstrated and validated through extensive analyses in 10-yrp, 100-yrp and 1,000-yrp hurricanes in ultra-deepwater central GoM.

Substituting oat hay or maize silage for portion of alfalfa hay affects growth performance, ruminal fermentation, and nutrient digestibility of weaned calves

Yang Zou,XinPing Zou,XiZhi Li,Gang Guo,Peng Ji,Yan Wang,ShengLi Li,YaJing Wang,ZhiJun Cao 아세아·태평양축산학회 2018 Animal Bioscience Vol.31 No.3

Objective: The impact of forage feeding strategy on growth performance, ruminal fermentation and nutrient digestibility in post-weaning calves was investigated. Methods: Forty-five female Holstein calves (body weight [BW] = 79.79±0.38 kg) were enrolled in the 35-d study at one week after weaning and randomly assigned to one of three dietary treatments. All diets were fed as total mixed ration containing 60% (dry matter [DM] basis) of basal starter feed and 40% (DM basis) of forage, but varied in composition of forage source including i) alfalfa (40% DM, AH); ii) alfalfa hay (26.7% DM)+oat hay (13.3% DM; OH); iii) alfalfa hay (26.7% DM)+corn silage (13.3% DM; WS). Results: Dry matter intake was not different among treatment groups (p>0.05). However, BW (p<0.05) and average daily gain (p<0.05) of calves fed AH and OH were greater than WS-fed calves, whereas heart girth was greater in OH-fed calves than those fed AH and WS (p<0.05). Ruminal fermentation parameters including proportion of butyric acid, acetated-to-propionate ratio, concentration of total volatile fatty acid, protozoal protein, bacterial protein, and microbial protein in rumen were the highest in OH (p<0.05) and the lowest in WS. Compared with the AH and WS, feeding oat hay to postweaning calves increased crude protein digestibility (p<0.05), and decreased duration of diarrhea (p<0.05) and fecal index (p<0.05). Conclusion: Our results suggested that partially replacing alfalfa hay with oat hay improved ruminal fermentation, nitrogen utilization, and reduced incidence of diarrhea in post-weaning dairy calves.

자기 공명 영상을 이용한 안면비대칭환자의 측두하악관절 원반의 형태와 위치에 관한 연구

Bingshuang Zou,김태우,최순철 대한치과교정학회 2005 대한치과교정학회지 Vol.35 No.5

본 연구는 안면비대칭이 있는 환자의 측두하악관절원반 형태와 위치를 평가하고 악관절내장증과 안명비대칭 간의 관계를 규명하기 위하여 시행되었다. 남자 10명 및 여자 27명으로 구성된 안명비대칭 환자 37명의 74개의 TMJ MRI를 사용하였으며, 대상 집단의 연령은 12세에서 44세까지의 범위로 평균 21.4세였다. 시상 및 두정 TMJ MRI를 최대감합위 및 최대개구위에서 채득한 후 부분전방변위, 전방변위, 회전 또는 측방 원반변위 등 원반변위의 종류를 기록하였으며, 원반의 모양, 위치, 그리고 원반의 변위 및 회전을 MRI tracing 상에서 판독하였다. 악관절내장증의 증상은 정상, 정복성 전방원반변위 (ADDR). 그리고 비정복성 전방원반변위(ADDNR)집단으로 분류하였으며, 환자는 양측성 정상. 편측 혹은 양측 내장증 집단으로 분류하였다. 약 70%의 환자에서 편측성 또는 양측성 내장증을 보였으며, 통계분석 결과 ADD. 특히 회전성 ADD가 변위측에서 더 높은 빈도로 나타났으나 반대측에서는 원반의 위치가 정상인 경우가 많았다 (p<0.01).변위측의 원반은 모양의 변형 및 하전방 변위를 유의하게 더 나타냈다. 그러나 반대쪽은 개구시 원반이 수직방향으로 과운동성이 관찰되었다. 이 연구는 안면비대칭 환자에서 ADD의 종류가 원반의 모양,경사,변위 각도,변위의 수직거리 및 회전각도와 연관이 있다는 것을 제시한다. The present study was conducted to examine the morphometrics and function of the disk on both sides among patients with facial asymmetry (FA) and to elucidate plausible correlations between internal derangement (ID) and FA. The sample was composed of 10 males and 27 females with FA. The disk status of all subjects was evaluated by biateral high resolution magnetic resonance scans in the sagittal (closed and open) and coronal (closed) planes. Five types of disk displacement were identified accordingly. The disk function was diagnosed as normal disk function, disk displacement with reduction, and disk displacement without reduction. The disk shape on sagittal MRI in closed position was classified as bi-concave, biplanar, funnel/hemiconvex, and deformed. The disk position, translation and rotation were also measured. The difference between the shifted side and non-shifted side was analyzed by statistical analysis. Approximately 70% of the patients in the present study showed unilateral or bilateral ID. It was found that anterior disk displacements (ADD), especially rotational ADD, occurred more frequently in the shifted side, while normal disk position was observed mainly in the non-shifted side(p<0.01). The disk of the shifted side showed significantly deformed configuration and inferior-anterior disk position. However, the disk of the non-deviated side showed hyper-mobility during jaw opening movement. These results demonstrate that in FA patients, the disks status of the shifted side is different from that of the non-shifted side, a phenomenon that could be correlated to facial asymmetry.

Analysis of the toroidal HVDC grounding systems in horizontal multilayer soils

Zou, J.,Liu, Y.Q.,Yuan, J.S.,He, J.L.,Cao, L.,Lee, J.,Chang, S. IEEE 2006 IEEE transactions on magnetics Vol.42 No.4

<P>A Fredholm integral equation of the first kind has been developed for analyzing the performance of the toroidal high-voltage direct current (HVDC) grounding system buried in the horizontal multilayer soil. The method of moment is used to solve the equations. The formulation of potential in the earth due to a circular current is proposed in closed form by means of conception of the complex resistivity and depth, which leads to the saving of the computational time greatly. The numerical results show the good agreements with those in other papers and the measured data</P>

Application of Three-phase Hollow Fiber LPME using an Ionic Liquid as Supported Phase for Preconcentration of Malachite Green from Water Samples with HPLC Detection

Zou, Yanmin,Zhang, Zhen,Shao, Xiaoling,Chen, Yao,Wu, Xiangyang,Yang, Liuqing,Zhu, Jingjing,Zhang, Dongmei Korean Chemical Society 2014 Bulletin of the Korean Chemical Society Vol.35 No.2

A novel three-phase hollow fiber liquid phase microextraction was developed for the determination of malachite green (MG) in environmental waters, which selected [BMIM][$PF_6$] mixed with 1% trioctylphosphine oxide (TOPO) as supported phase. Several parameters (accepter phase pH, sample pH, supported phase membrane, volume of accepter phase, salinity, extraction time) that could affect extraction performance were investigated. Under the optimal extraction conditions, the established approach showed excellent characters as: high enrichment factor (212), wide linear range ($0.20-100{\mu}gL^{-1}$), low detection limit ($0.01{\mu}gL^{-1}$), good reproducibility (RSD, 8.9%, n=5) and satisfactory recovery (84.0-106.2%). The method was applied to detect MG at Yangtze River and pond waters in Zhenjiang, Jiangsu province, and 4 sites among 15 sampling sites were found MG with the concentration of $1.73-11.06{\mu}gL^{-1}$, which confirmed that the proposed environmentally friendly method was simple and effective for monitoring MG in aquatic system.

SIRT3-Mediated Dimerization of IDH2 Directs Cancer Cell Metabolism and Tumor Growth

Zou, Xianghui,Zhu, Yueming,Park, Seong-Hoon,Liu, Guoxiang,O'Brien, Joseph,Jiang, Haiyan,Gius, David American Association for Cancer Research 2017 Cancer research Vol.77 No.15

<P>These findings identify SIRT3 as a potential tumor suppressor, mediating its effects by limiting the ability of IDH2 to drive cancer cell metabolism and malignant progression.</P><P>The isocitrate dehydrogenase IDH2 produces α-ketoglutarate by oxidizing isocitrate, linking glucose metabolism to oxidative phosphorylation. In this study, we report that loss of SIRT3 increases acetylation of IDH2 at lysine 413 (IDH2-K413-Ac), thereby decreasing its enzymatic activity by reducing IDH2 dimer formation. Expressing a genetic acetylation mimetic IDH2 mutant (IDH2<SUP>K413Q</SUP>) in cancer cells decreased IDH2 dimerization and enzymatic activity and increased cellular reactive oxygen species and glycolysis, suggesting a shift in mitochondrial metabolism. Concurrently, overexpression of IDH2<SUP>K413Q</SUP> promoted cell transformation and tumorigenesis in nude mice, resulting in a tumor-permissive phenotype. IHC staining showed that IDH2 acetylation was elevated in high-risk luminal B patients relative to low-risk luminal A patients. Overall, these results suggest a potential relationship between SIRT3 enzymatic activity, IDH2-K413 acetylation-determined dimerization, and a cancer-permissive phenotype. <I>Cancer Res; 77(15); 3990–9. ©2017 AACR</I>.</P>

Responses to Starch Infusion on Milk Synthesis in Low Yield Lactating Dairy Cows

Zou, Yang,Yang, Zhanshan,Guo, Yongqing,Li, Shengli,Cao, Zhijun Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.9

The effect of starch infusion on production, metabolic parameters and relative mRNA abundance was investigated in low yield lactating cows from 86 days in milk. Six Holstein cows fitted with permanent ruminal cannulas were arranged into one of two complete $3{\times}3$ Latin squares and infused with a starch solution containing 800 grams starch for 16 days. The three treatments were: i) ruminal and abomasal infusion with water (Control); ii) ruminal infusion with cornstarch solution and abomasal infusion with water (Rumen); iii) ruminal infusion with water and abomasal infusion with cornstarch solution (Abomasum). There were no significant differences (p>0.05) among the three treatments with low yield lactating cows in feed and energy intake, milk yield and composition, plasma metabolism, or even on gene expression. However, cows receiving starch through rumen performed better than directly through the abomasum during the glucose tolerance test procedure with a higher area under the curve (AUC; p = 0.08) and shorter half-time ($t^{1/2}$; p = 0.11) of plasma insulin, therefore, it increased glucose disposal, which stated a lipid anabolism other than mobilization after energy supplementation. In conclusion, extra starch infusion at concentration of 800 g/d did not enhance energy supplies to the mammary gland and improve the lactating performance in low yield lactating cows.

In vitro disinfection efficacy and clinical protective effects of common disinfectants against acute hepatopancreatic necrosis disease (AHPND)- causing Vibrio isolates in Pacific white shrimp Penaeus vannamei

Zou Peizhuo,Yang Qian,Wang Hailiang,Xie Guosi,Cao Zhi,Chen Xing,Gao Wen,Huang Jie 한국미생물학회 2020 The journal of microbiology Vol.58 No.8

Acute hepatopancreatic necrosis disease (AHPND) is one of the most significant bacterial diseases in global shrimp culture, causing severe economic losses. In the present study, we carried out in vitro antimicrobial tests to investigate the disinfection efficacy of 14 common disinfectants toward different AHPND-causing Vibrio spp., including eight isolates of V. parahaemolyticus, four isolates of V. campbellii, and one isolate of V. owensii. Polyhexamethylene biguanidine hydrochloride (PHMB) was revealed to possess the strongest inhibitory activity. Through analyzing and evaluating the results of antimicrobial tests and acute toxicity test, we selected PHMB and hydrogen peroxide (H2O2) for further clinical protection test. Clinical manifestations indicated that both PHMB (2 mg/L and 4 mg/L) and H2O2 (12 mg/L) could effectively protect juvenile Penaeus vannamei from the infection of V. parahaemolyticus isolate Vp362 at 106 CFU/ml, and the survival rate was over 80%. When the bacterial concentration was reduced to 105 CFU/ml, 104 CFU/ml, and 103 CFU/ml, the survival rate after treated by 1 mg/L PHMB was 64.44%, 93.33%, and 100%, respectively. According to the results, PHMB and H2O2 showed a lower toxicity while a better protection activity, particularly against a lower concentration of the pathogens. Therefore, these two disinfectants are proved to be promising disinfectants that can be applied to prevent and control AHPND in shrimp culture. Moreover, the methods of this study also provided valuable information for the prevention of other important bacterial diseases and suggested a reliable means for screening potential drugs in aquaculture.