Correlation between DNA methylation and Thymic Stromal Lymphopoietin expression in asthmatic airway epithelial cells

Yan‑Li Li,Xi‑Qian Xing,Yi Xiao,Yan‑Hong Liu,Yu‑Shan Zhou,Min Zhuang,Chao‑Qian Li 한국유전학회 2020 Genes & Genomics Vol.42 No.12

Background: The overexpression of TSLP and DNA methylation in asthma were both risk factors the relationship was not clear. Objective: This study aimed to investigate the relationship between methylation status of TSLP promoter and mRNA/protein expression in asthmatic airway epithelial cells. Methods: Human bronchial epithelial cells were cultured in vitro and divided into: Control group, treated with PBS, model group, sensitized with LPS (10 μg/mL) for 12 h (37 °C, 5% CO2). Other groups were cultured with the pCMV3 plasmid (M + NC/pCMV), pGPH1 plasmid (M + NC/pGPH), DNMT1/pCMV3 plasmid (M + DNMT1/pCMV), and DNMT1/pGPH1 plasmid (M + DNMT1/pGPH) for 48 h. The expression of DNA methyltransferase 1 and TSLP were measured using real-time PCR and western blotting. Results: Compared with the control group, TSLP mRNA (1.00 ± 0.00 vs. 2.82 ± 0.81 vs. 1, P < 0.001) and protein (1.07 ± 0.04 vs. 1.46 ± 0.11, P < 0.01) were significantly greater, and the methylation of promoter was lower (92.75 ± 1.26 vs. 58.57 ± 3.34, P < 0.05) in the model group. Compared with the model group, TSLP mRNA (2.82 ± 0.81 vs. 1.17 ± 0.10, P < 0.001) decreased, but TSLP promoter methylation increased (58.57 ± 3.34 vs. 92.58 ± 7.30, P < 0.05) in M + DNMT1/pCMV. TSLP mRNA and protein were higher (2.82 ± 0.81 vs. 5.32 ± 0.21, P < 0.001; 1.46 ± 0.11 vs. 1.94 ± 0.11, respectively, P < 0.01), TSLP promoter methylation was lower (58.57 ± 3.34 vs. 33.57 ± 4.29, P < 0.05) in M + DNMT1/pGPH. Conclusions: Overexpression of TSLP in asthmatic airway epithelial cells may be regulated by DNA demethylation.

LPL gene Pvu II polymorphism and hypertriglycer-idemia: a meta-analysis involving 1,640 subjects

( Yan-yan Li ),( Yan-hong Zhou ),( Ge Gong ),( Hong-yu Geng ),( Xin-xing Yang ),( Xiang-ming Wang ),( Chuan-wei Zhou ),( Jian Xu ),( Yun Qian ) 대한내과학회 2017 The Korean Journal of Internal Medicine Vol.32 No.6

Background/Aims: Although lipoprotein lipase (LPL) gene Pvu II polymorphism has been associated with an increased risk of hypertriglyceridemia (HT), there is no clear consensus within the scientific community. Methods: A meta-analysis of 1,640 subjects from six individual studies was conducted to better elucidate the potential relationship between the LPL gene Pvu II polymorphism and HT within the Chinese population. Pooled odds ratios (ORs) and their corresponding 95% confidence intervals (CIs) were evaluated by using fixed effect models. Results: Our analysis indicated a significant association between LPL gene Pvu II polymorphism and HT within the Chinese population under allelic (OR, 1.550; 95% CI, 1.320 to 1.830; p = 1.158 × 10^-7), recessive (OR, 0.540; 95% CI, 0.390 to 0.750; p = 0.0002), dominant (OR, 1.889; 95% CI, 1.501 to 2.377; p = 5.960 × 10^-8), homozygous (OR, 2.167; 95% CI, 1.531 to 3.067; p = 1.242 × 10^-5), heterozygous (OR, 1.810; 95% CI, 1.419 to 2.309; p = 1.842 × 10^-6), and additive genetic models (OR, 1.553; 95% CI, 1.320 to 1.828; p = 1.158 × 10^-7). Conclusions: Because LPL gene Pvu II restriction fragment length polymorphism polymorphism was associated with an elevated risk of HT, the P+ allele carriers of the LPL gene might be predisposed to HT.

Analytical solutions to piezoelectric bimorphs based on improved FSDT beam model

Zhou, Yan-Guo,Chen, Yun-Min,Ding, Hao-Jiang Techno-Press 2005 Smart Structures and Systems, An International Jou Vol.1 No.3

This paper presents an efficient and accurate coupled beam model for piezoelectric bimorphs based on improved first-order shear deformation theory (FSDT). The model combines the equivalent single layer approach for the mechanical displacements and a layerwise modeling for the electric potential. General electric field function is proposed to reasonably approximate the through-the-thickness distribution of the applied and induced electric potentials. Layerwise defined shear correction factor (k) accounting for nonlinear shear strain distribution is introduced into both the shear stress resultant and the electric displacement integration. Analytical solutions for free vibrations and forced response under electromechanical loads are obtained for the simply supported piezoelectric bimorphs with series or parallel arrangement, and the numerical results for various length-to-thickness ratios are compared with the exact two-dimensional piezoelasticity solution. Excellent predictions with low error estimates of local and global responses as well as the modal frequencies are observed.

Capacitance Enhanced Synchronization of Pairs of Spin-transfer Oscillators

Yan Zhou,Bo Guan,F. G. Shin,Johan Akerman 한국자기학회 2008 한국자기학회 학술연구발표회 논문개요집 Vol.- No.-

Spin-torque Oscillator with Tilted Fixed Layer Magnetization

Yan Zhou,S. Bonetti,C. L. Zha,J. Persson,Johan Akerman 한국자기학회 2008 한국자기학회 학술연구발표회 논문개요집 Vol.- No.-

Field-Like Spin Torque Term Governs Enhanced Synchronization in Magnetic Tunnel Junction based Spin Torque Oscillator

Yan Zhou,Johan Akerman 한국자기학회 2008 한국자기학회 학술연구발표회 논문개요집 Vol.- No.-

Synergistic Penetration of Ethosomes and Lipophilic Prodrug on the Transdermal Delivery of Acyclovir

Yan Zhou,Yu-Hui Wei,Guo-Qiang Zhang,Xin-An Wu 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.4

The aim of this study was to investigate the lipophilic prodrug as a means of promoting acyclovir (ACV) that exhibited biphasic insolubility into the ethosomes for optimum skin delivery. Acyclovir Palmitate (ACV-C16) was synthesized as the lipophilic prodrug of ACV. The ethosomal system and the liposomal system bearing ACV or ACV-C16 were prepared, respectively. The systems were characterized for shape, zeta potential value, particle size, and entrapment efficiency. Franz diffusion cells and confocal laser scanning microscopy were used for the percutaneous absorption studies. The results showed that the entrapment efficiency of ACV-C16ethosomes (87.75%) were much higher than that of ACV ethosomes (39.13%). The quantity of drug in the skin from ACV-C16 ethosomes at the end of the 24 h transdermal experiment (622.89 μg/cm2) was 5.30 and 3.43 times higher than that from ACV-C16 hydroalcoholic solution and ACV ethosomes, respectively. This study indicated that the binary combination of the lipophilic prodrug ACV-C16 and the ethosomes synergistically enhanced ACV absorption into the skin.

『源氏物語』と『紅楼夢』についての比較研究

Zhou Yan shu(周硯舒) 고려대학교 글로벌일본연구원 2014 일본연구 Vol.22 No.-

A century ago, The Tale of Gengi created by Murasakisikibu became the masterpiece of Japanese literature. Seven hundred years later, The Dream of the Red Chamber written by Cao Xueqin and Gao'e led Chinese classic literature to its peak. Although there was a long period between the two great works, there are many similarities with their major female characters: Lin Daiyu, Xue Baochai and Murasakinoue. All three were born in royal families with a bitter childhood and with the loss of parent. Beautiful and talented as they were, they suffered to the end. Nevertheless, they were different in their family backgrounds, deaths, and love. Murasakinoue was obedient and loyal to the society represented by Genji as a kind and reverent woman, while Daiyu had a strong aversion to hypocritical scholarly honor and social ranks, pursing her ideal love freely under the feudalistic marriage system, as a social rebel, but Baochai was another type with no love for Baoyu at all. But the most important thing is that the three characters had their unique personalities. In particular, as the ideal women figures under the social system, Baochai and Murasakinoue differed essentially. Baochai was sophisticated, thoughtful, and obedient but cold and snobbish. Similar to Baochai, Murasakinoue was integral and pure, which was actually very similar to Lin Daiyu. Compared with Baochai and Murasakinoue, Daiyu was a feature with flaws, but it is the flaws that make Daiyu lively. In The Tale of Genji, Murasakinoue is compared to sakura, and Daiyu and Baocha are compared to Furong and Mudan in The Dream of the Red Chamber . Sakura is the national flower of Japan, while Mudan and Furong are regarded in Chinese culture as the king of the flowers and the Flower Fairy. It is true that the three flowers root in various lands, but they are all well known for their fragrance. Based on the discussion about the similarities and differences in the three major female characters, the article intends to make a further study on the women's view of the authors. 『源氏物語』は、11世紀の初め、紫式部によって完成され、日本の古典文学の最高傑作だといわれている。『紅楼夢』は中国の清の時代、曹雪芹と高鶚によって完成され、中国の文学史上の頂点だと言える。この二作は、成立年代がかなりはなれているが、相似するところが多い。そして、この二作のヒロインと言える林黛玉、薛宝釵と紫の上についても、似ているところがたくさんある。大きく見れば、三人とも貴族の出身で、幼い時両親またはその一人が亡くなった。そして、三人とも才色兼備な女性であったが、どちらも結末は不幸であった。しかし仔細に見れば、この三人の身の上、結末についてもやはり違いがある。そして、恋愛についてもそれぞれである。紫の上は光源氏またはそのような社会には本心をもっての従順者で、本当の温良恭倹の人である。黛玉は虚偽な功名が嫌いで、婚姻の不自由な制度の下でも自由で理想的な愛情を求め、その社会の反逆児だとも言える。宝钗は紫の上また黛玉とは違う。宝玉には激しい愛情が見えない。また、最も重要なのは、三人の性格は異なっている。特に、紫の上と宝釵は古代の保守的制度の下で理想的な女性とされているが、二人には実は本質的な違いがある。宝钗は実は思慮深く、世渡り上手の賢い人である。表は温良恭倹のように見えるが、裏では冷たくて、功名心のある人である。宝钗と紫の上とは表面上において似ているが、素直で清い心をもっているほうから見ると、むしろ黛玉と紫の上は同様だとは言えよう。黛玉は宝釵と紫の上に比べたら、欠点のある人である。そしてこの欠点があるからこそ、黛玉は小説の中の人のように見えなく、現実の中で生きている人のように見える。『源氏物語』は、紫の上を桜とたとえている。『紅楼夢』は、林黛玉を芙蓉、薛宝釵を牡丹に比喩している。この三つの花は、違う土壌に生まれ育てられたが、同様に美しく芳しい花が咲いた。しかし、それぞれの特質を持っている。拙作は、林黛玉、薛宝釵と紫の上の相似と相違について探究することを試みた論

Feasibility of a Clinical-Radiomics Model to Predict the Outcomes of Acute Ischemic Stroke

Zhou Yiran,Wu Di,Yan Su,Xie Yan,Zhang Shun,Lv Wenzhi,Qin Yuanyuan,Liu Yufei,Liu Chengxia,Lu Jun,Li Jia,Zhu Hongquan,Liu Weiyin Vivian,Liu Huan,Zhang Guiling,Zhu Wenzhen 대한영상의학회 2022 Korean Journal of Radiology Vol.23 No.8

Objective: To develop a model incorporating radiomic features and clinical factors to accurately predict acute ischemic stroke (AIS) outcomes. Materials and Methods: Data from 522 AIS patients (382 male [73.2%]; mean age ± standard deviation, 58.9 ± 11.5 years) were randomly divided into the training (n = 311) and validation cohorts (n = 211). According to the modified Rankin Scale (mRS) at 6 months after hospital discharge, prognosis was dichotomized into good (mRS ≤ 2) and poor (mRS > 2); 1310 radiomics features were extracted from diffusion-weighted imaging and apparent diffusion coefficient maps. The minimum redundancy maximum relevance algorithm and the least absolute shrinkage and selection operator logistic regression method were implemented to select the features and establish a radiomics model. Univariable and multivariable logistic regression analyses were performed to identify the clinical factors and construct a clinical model. Ultimately, a multivariable logistic regression analysis incorporating independent clinical factors and radiomics score was implemented to establish the final combined prediction model using a backward step-down selection procedure, and a clinical-radiomics nomogram was developed. The models were evaluated using calibration, receiver operating characteristic (ROC), and decision curve analyses. Results: Age, sex, stroke history, diabetes, baseline mRS, baseline National Institutes of Health Stroke Scale score, and radiomics score were independent predictors of AIS outcomes. The area under the ROC curve of the clinical-radiomics model was 0.868 (95% confidence interval, 0.825–0.910) in the training cohort and 0.890 (0.844–0.936) in the validation cohort, which was significantly larger than that of the clinical or radiomics models. The clinical radiomics nomogram was well calibrated (p > 0.05). The decision curve analysis indicated its clinical usefulness. Conclusion: The clinical-radiomics model outperformed individual clinical or radiomics models and achieved satisfactory performance in predicting AIS outcome

Increased brain uptake of venlafaxine loaded solid lipid nanoparticles by overcoming the efflux function and expression of P-gp

Yan Zhou,Xin’an Wu,Guo-Qiang Zhang,Zhi Rao,Yang Yang,Qian Zhou,Hongyan Qin,Yuhui Wei 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.7

Venlafaxine (VLX) could be pumped out of the brain by P-glycoprotein (P-gp). Moreover, the expression of P-gp distributed in blood–brain barrier could be significantly induced by VLX. Thus, P-gp could be considered as the nature barrier for delivering of VLX to the brain. The aim of this study was to investigate whether the efflux function and increased expression of P-gp could be reversed by utilizing solid lipid nanoparticles (SLN). VLX solid lipid nanoparticles (VLX - SLN) were prepared and evaluated. Pharmacokinetics and brain distribution of VLX in different formulations were conducted after oral or intravenous administration. P-gp efflux function to VLX was evaluated by the brain uptake amount of VLX, while P-gp expression was investigated by Western blotting. Results indicated that the entrapment, mean size and zata potential of VLX - SLN was 74.9 ± 3.0 %, 186.3 ± 69.26 nm and -22.8 ± 7.78 mv, respectively. After vein injection of VLX formulations, the brain uptake amount of VLX from VLX - SLN was significantly higher than that of VLX solution, VLX solution with empty SLN (VLX? empty SLN) and VLX solution with Verapamil (VLX ? Ver), respectively. Furthermore, the protein mass of P-gp in VLX - SLN treated group was the lowest among all the investigated groups. These results indicated that SLN could overcome P-gp and achieve brain target by intravenous administration.