Preparation and characterization of poly(dimethylsiloxane)-polytetrafluoroethylene (PDMS-PTFE) composite membrane for pervaporation of chloroform from aqueous solution

Zhen-Liang Xu,De Sun,Bing-Bing Li 한국화학공학회 2013 Korean Journal of Chemical Engineering Vol.30 No.11

Hydrophobic polydimethylsiloxane - polytetrafluoroethylene (PDMS-PTFE) flat-sheet membranes for pervaporation (PV) of chloroform from aqueous solution were successfully fabricated by solution casting method. The structures and the performance of the membranes was characterized by X-ray diffraction (XRD), scanning electron microscope combined with energy dispersive X-ray spectroscopy (SEM-EDXS), Fourier transform infrared spectroscopy (FT-IR), thermal gravimetric analysis (TGA) and the tests of contact angle and mechanical properties. The adding of PTFE particles (<4 μm) in the PDMS matrix enhanced the crystallinity, hydrophobicity, mechanical strength and thermal stability of the membranes. The examinations showed that the PTFE filled PDMS membranes exhibited striking advantages in flux and separation factor as compared with unfilled PDMS membranes. All the filled PDMS membranes with different PTFE content showed excellent PV properties for the separation of chloroform from water. When the content of the PTFE additive in PDMS composite membrane was 30 wt%, membrane performance was the best at feed temperature 50 oC and permeate-side vacuum 0.101MPa. For the 30% PTFE-PDMS membrane, with the increase of the feed temperature from 30 to 60 oC, the total, water and chloroform fluxes as well as the separation factor increased,the apparent activation energy (ΔEa) of total, chloroform and water were 21.08, 66.65 and 11.49 KJ/mol, respectively,with an increase of chloroform concentration in the feed from 50 to 950 ppm, total, water and chloroform fluxes increased but the separation factor decreased.

MicroRNA-576-3p Inhibits Proliferation in Bladder Cancer Cells by Targeting Cyclin D1

Liang, Zhen,Li, Shiqi,Xu, Xin,Xu, Xianglai,Wang, Xiao,Wu, Jian,Zhu, Yi,Hu, Zhenghui,Lin, Yiwei,Mao, Yeqing,Chen, Hong,Luo, Jindan,Liu, Ben,Zheng, Xiangyi,Xie, Liping Korean Society for Molecular and Cellular Biology 2015 Molecules and cells Vol.38 No.2

MicroRNAs (miRNAs) are small, endogenous RNAs that play important gene-regulatory roles by binding to the imperfectly complementary sequences at the 3'-UTR of mRNAs and directing their gene expression. Here, we first discovered that miR-576-3p was down-regulated in human bladder cancer cell lines compared with the non-malignant cell line. To better characterize the role of miR-576-3p in bladder cancer cells, we over-expressed or down-regulated miR-576-3p in bladder cancer cells by transfecting with chemically synthesized mimic or inhibitor. The overexpression of miR-576-3p remarkably inhibited cell proliferation via G1-phase arrest, and decreased both mRNA and protein levels of cyclin D1 which played a key role in G1/S phase transition. The knock-down of miR-576-3p significantly promoted the proliferation of bladder cancer cells by accelerating the progression of cell cycle and increased the expression of cyclin D1. Moreover, the dual-luciferase reporter assays indicated that miR-576-3p could directly target cyclin D1 through binding its 3'-UTR. All the results demonstrated that miR-576-3p might be a novel suppressor of bladder cancer cell proliferation through targeting cyclin D1.

Is Surgical Resection Justified for Hepatocellular Carcinoma with Portal Vein Tumor Thrombus? (A Systematic Review and Meta-Analysis)

( Liang Lei ),( Xin-fei Xu ),( Jiong-jie Yu ),( Ju-dong Li ),( Zhen-li Li ),( Jun Han ),( Han Zhang ),( Hao Xing ),( Han Wu ),( Ming-da Wang ),( Chao Li ),( Zheng Wang ),( Feng Shen ),( Meng-chao Wu ) 대한간학회 2018 춘·추계 학술대회 (KASL) Vol.2018 No.1

Aims: The prognosis of hepatocellular carcinoma (HCC) with portal vein tumor thrombus (PVTT) is very poor. According to the BCLC treatment recommendations, sorafenib or other palliative treatment (PT) is recommended as the first-line therapy when it happens. In real world, however, a significant number of selected patients with HCC and PVTT suffered from surgical resection (SR). Methods: PubMed, Embase, Medline and Cochrane library were searched for studies comparing SR with PT (including TACE, sorafenib, etc.) for HCC with PVTT, which were published before September 2017. Results: 4,810 patients from 7 studies were enrolled in this meta-analysis, which divided into the SR group (n = 2,344) and the PT group (n = 2476). When compared with the PT group, the pooled hazard ratio (HR) for the 1, 3 and 5-year OS rates of the SR group were 0.56 (95% CI 0.52-0.60, P=0.03), 0.56 (95% CI 0.53-0.59, P<0.001) and 0.55 (95% CI 0.54-0.57, P<0.001). For subgroup analysis, when compared with the mere TACE group, the pooled HR for the 1, 3 and 5-year OS rates of the SR group were 0.54 (95% CI 0.43-0.67, P=0.81), 0.75 (95% CI 0.65-0.87, P=0.25) and 0.76 (95% CI 0.67-0.88, P=0.25). Conclusions: This meta-analysis demonstrated SR had better OS than TACE or other palliative therapy for HCC with PVTT. SR may be suitable as the first-line treatment for selected patients with resectable HCC and removable PVTT.

Association Between GSTM1 Polymorphism and Nasopharyngeal Cancer Susceptibility: a Meta-analysis

Sun, Zhen-Feng,Zhang, Jia,Xu, Hong-Ming,Wang, Guo-Liang,Dong, Pin Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.11

Background/Aims: Glutathione S-transferase M1 (GSTM1) is a multifunctional enzyme that plays a critical role in the detoxification of varieties of carcinogenic metabolites. Many studies have been conducted to investigate the association between GSTM1 polymorphism and nasopharyngeal cancer (NPC) risk, but the findings among those studies are inconsistent. To assess this relationship more precisely, we performed a meta-analysis of all available studies on the subject. Methods: Case-control studies were identified by searching Pubmed, Embase, ISI Web of Science, and Wanfang databases through September 6, 2012. We used the pooled odds ratio (OR) with its corresponding 95% confidence interval (95%CI) to evaluate the association of GSTM1 polymorphism with NPC susceptibility. Subgroup analyses by pathological types, sex and smoking status were performed to further identify the association. Results: Overall, 11 published studies with 1,513 cases and 2,802 controls were finally included into this meta-analysis according to the inclusion criteria. Meta-analysis of total studies showed that the null genotype of GSTM1 was significantly associated with increased risk of NPC, when comparing with the non-null genotype (OR=1.51, 95%CI=1.33-1.72, POR<0.001). The association was still statistically significant in subgroup analysis of patients with nasopharyngeal squamous cell carcinoma (OR=1.73, 95%CI=1.24-2.42, POR=0.001). Males with the null genotype of GSTM1 were more likely to subject to NPC than females. In addition, the association between the null genotype of GSTM1 and NPC risk was strongest in individuals with exposure to smoking. Sensitivity analysis by sequential omission of any individual studies one at a time further demonstrated the significant association. Conclusions: The findings suggest that the null genotype of GSTM1 is a risk factor for NPC, and there is a gene-smoking interaction in this association.

Excellent anti-fouling performance of PVDF polymeric membrane modified by enhanced CaA gel-layer

Sun-Jie Xu,Gui-E Chena,Zhen-Liang Xu 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.58 No.-

Calcium alginate gel-layer polymeric membranes with excellent anti-fouling ability were developed viadip-coating method through sodium alginate and calcium chloride to form gel-layers onto differentpolyvinylidenefluoride membranes. SEM, FT-IR, DCA and mechanical properties were operated forcharacterization. Comprehensive investigation of anti-fouling ability including PWF, FRR%, long-termperformances with fouling resistances were conducted. Results showed a significant improvement aftercross-linking, in which recovery rate reached to 99.2% and kept steady while contact angle decreasedremarkably. Enhanced gel-layer with MWCNTs-COOH was also developed and applied in the commercialPVDF MF membrane and applied in fresh milk emulsion concentration.

MicroRNA-576-3p Inhibits Proliferation in Bladder Cancer Cells by Targeting Cyclin D1

Liping Xie,Zhen Liang,Shiqi Li,Xin Xu,Xianglai Xu,Xiao Wang,Jian Wu,Yi Zhu,Zhenghui Hu,Yiwei Lin,Yeqing Mao,Hong Chen,Jindan Luo,Ben Liu,Xiangyi Zheng 한국분자세포생물학회 2015 Molecules and cells Vol.38 No.2

MicroRNAs (miRNAs) are small, endogenous RNAs that play important gene-regulatory roles by binding to the imperfectly complementary sequences at the 3 -UTR of mRNAs and directing their gene expression. Here, we first discovered that miR-576-3p was down-regulated in human bladder cancer cell lines compared with the non-malignant cell line. To better characterize the role of miR-576-3p in bladder cancer cells, we over-expressed or down-regulated miR-576-3p in bladder cancer cells by transfecting with chemically synthesized mimic or inhibitor. The overexpression of miR-576-3p remarkably inhibited cell proliferation via G1-phase arrest, and decreased both mRNA and protein levels of cyclin D1 which played a key role in G1/S phase transition. The knock-down of miR-576-3p significantly promoted the proliferation of bladder cancer cells by accelerating the progression of cell cycle and increased the expression of cyclin D1. Moreover, the dual-luciferase reporter assays indicated that miR-576-3p could directly target cyclin D1 through binding its 3 -UTR. All the results demonstrated that miR-576-3p might be a novel suppressor of bladder cancer cell proliferation through targeting cyclin D1.

Cryptotanshinone Induces Inhibition of Breast Tumor Growth by Cytotoxic CD4+ T Cells through the JAK2/STAT4/ Perforin Pathway

Zhou, Jun,Xu, Xiao-Zhen,Hu, Yao-Ren,Hu, Ai-Rong,Zhu, Cheng-Liang,Gao, Guo-Sheng Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.6

Cryptotanshinone (CPT), is a quinoid diterpene isolated from the root of the Asian medicinal plant, Salvia miotiorrhiza bunge. Numerous researchers have found that it could work as a potent antitumor agent to inhibit tumor growth in vitro, buith there has been much less emphasis on its in vivo role against breast tumors. Using a mouse tumor model of MCF7 cells, we showed that CPT strongly inhibited MCF7 cell growth in vivo with polarization of immune reactions toward Th1-type responses, stimulation of naive CD4+ T cell proliferation, and also increased IFN-${\gamma}$ and perforin production of CD4+ T cells in response to tumor-activated splenocytes. Furthermore, data revealed that the cytotoxic activity of CD4+ T cells induced by CPT was markedly abrogated by concanamycin A(CMA), a perforin inhibitor, but not IFN-${\gamma}$ Ab. On the other hand, after depletion of CD4+ T cells or blocked perforin with CMA in a tumor-bearing model, CPT could not effectively suppress tumor growth, but this phenomenon could be reversed by injecting naive CD4+ T cells. Thus, our results suggested that CPT mainly inhibited breast tumor growth through inducing cytotoxic CD4+ T cells to secrete perforin. We further found that CPT enhanced perforin production of CD4+ T cells by up-regulating JAK2 and STAT4 phosphorylation. These findings suggest a novel potential therapeutic role for CPT in tumor therapy, and demonstrate that CPT performs its antitumor functions through cytotoxic CD4+ T cells.

Solvothermal Synthesis of Bi₂O₂CO₃ Nanoplates for Efficient Photodegradation of RhB and Phenol under Simulated Solar Light Irradiation

Hu, Sheng-Peng,Xu, Cheng-Yan,Zhang, Bao-You,Pei, Yi,Zhen, Liang Korean Chemical Society 2014 Bulletin of the Korean Chemical Society Vol.35 No.10

Monodispersed $Bi_2O_2CO_3$ nanoplates with an average width of 320 nm and thicknesses of 50-90 nm were successfully synthesized by a simple solvothermal method in a mixture solution of polyethylene glycol and $H_2O$. The obtained nanoplates were characterized by means of XRD, FT-IR, SEM and TEM. The effect of surfactant sodium dodecyl benzene sulfonate on the morphology of $Bi_2O_2CO_3$ product was investigated. Under simulated solar light irradiation, $Bi_2O_2CO_3$ nanoplates exhibited superior photocatalytic activities towards the degradation of RhB as well as high chemical stability upon cycling photocatalytic test. The nanoplates also showed promising photodegradation ability for eliminating refractory pollutant of phenol. The excellent photocatalytic performance of $Bi_2O_2CO_3$ nanoplates as compared with P25-$TiO_2$ endows them as promising high efficiency photocatalysts.

ZnO Nanowires/ CdS Nanorods Structure Grown on Silica Micropillars Array for Photosensitive Application

Jing Liu,Yuanze Xu,Xiaoxiao Liang,Mingming Yan,Bo Wang,Tianchong Zhang,Zhen Hong,Futing Yi 대한금속·재료학회 2021 ELECTRONIC MATERIALS LETTERS Vol.17 No.6

Utilizing silica micropillar arrays as substrate, a kind of Zinc oxide (ZnO) nanowires/Cadmium sulfi de (CdS) nanopillars heterojunctionstructure is prepared successfully by twice hydrothermal reaction method. This ZnO nanowires/CdS nanorods/silicamicropillars structure is tree-liked morphology from the SEM and TEM images, and both the ZnO nanowires and CdS nanorodsare well crystallinity on the silica surface from the X-ray diff raction curves. The CdS nanorods/silica micropillars structure has alow refl ectivity to the incoming light for wavelength from 200 to 800 nm. After the ZnO nanowires growing, the refl ectivity oftree-liked structure is much lower than that without ZnO nanowires especially for the wavelength less than 350 nm. The resistanceresponse of the CdS nanorods/silica micropillars based photoresistors with and without ZnO nanowires are tested. And theresults reveal that the ZnO nanowires covering can improve the response of the CdS nanorods/silica micropillars photoresistors. The best photosensitivity response of the ZnO nanowires/CdS nanorods/silica micropillars photoresistor for 450 nm wavelengthlight with 5 mW/cm 2 illumination can achieve to 23.3, while that for CdS nanorods/silica micropillars photoresistors is 18.8.

Effect of polymer and additive on the structure and property of porous stainless steel hollow fiber

Xiao-Hua Ma,Zhen-Liang Xu,Yu Bai,Yue Cao 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.8

Porous stainless steel hollow fiber has been widely used due to its high mechanical strength, excellentthermal conductivity and good sealing properties compared with other porous supports. We successfully prepared porousstainless steel hollow fibers using polyacrylonitrile (PAN) as polymer via dry-wet spinning followed by sintering throughtemperature programming method. The PAN concentration had an obvious impact on the structure and property ofporous stainless steel hollow fiber even if it would be burned off during sintering. The results showed that the morphologycould be tuned by adjusting the concentration of PAN. With increasing PAN concentration in casting solutionfor spinning, the viscosity was increased dramatically, resulting in much compact structures with high pure water flux(higher than 3×105 L·m−2·h−1·Pa−1). A more dense structure could be obtained by adding additive polyvinylpyrrolidone(PVP) as viscosity enhancer.