2-[(4-Cyanophenyl)amino]-3-chloro-1,4-naphthalenedione (NQ-Y15)의 돌연변이원성

김봉희,정기화,유충규,창동신,이기선,전선덕,소동수,채상호,문창규 德成女子大學校 藥學硏究所 2001 藥學論文誌 Vol.12 No.1

2-[(4-Cyanophenyl)amino]-3-chloro-1, 4-naphthalenedione (NQ-Y15) was asssayed for its genotoxic potential by using Salmonella typhimurium reversion assay and in vitro chromosome aberration test on Chinese hamster lung cells. In the Ames test, NQ-Y15 induced his+revertants of Salmonella typhimurium TA 98 and TA 1537, reaching levels twice the negative control values. But, NQ-Y15 induced only his+revertants of Salmonella typhimurium TA 1537 more than twice the control values under the condition with metabolic activation system. In the cytogenetic test on chinese hamster lung cells. NQ-Y15 showed significant chromosomal aberrations, but the incidence was significantly reduced in the presence of metabolic activation.

2-[(4-Cyanophenyl)amino]-3-chloro-1,4-naphthalenedione(NQ-Y15)의 돌연변이원성

김봉희,정기화,유충규,창동신,이기선,전선덕,소동수,채상호,문창규 한국환경독성학회 2000 환경독성보건학회지 Vol.15 No.4

2-[(4-Cyanophenyl)amino]-3-chloro-1, 4-naphthalenedione (NQ-Y15) was asssayed for its genotoxic potential by using Salmonella typhimurium reversion assay and in vitro chromosome aberration test on Chinese hamster lung cells. In the Ames test, NQ-Y 15 induced his+ revertants of Salmonella typhimurium TA 98 and TA1537, reaching levels twice the negative control values. But, NQ-Y15 induced only his^(+) revertants of Salmonella typhimurium TA1537 more than twice the control values under the condition with metabolic activation system. In the cytogenetic test on chinese hamster lung cells, NQ-Y15 showed significant chromosomal aberrations, but the incidence was significantly reduced in the presence of metabolic activation.

Enhancing Cross-Cultural Adaptation Skills of International Chinese Language Teachers

Zhang Lin(Lin Zhang),Sun Yi(Yi Sun) 아시아사회과학학회 2023 Jornal of Asia Social Science Vol.10 No.2

Since 2004, China National Office for Teaching Chinese as a Foreign Language began dispatching international Chinese language teachers to countries in need around the world, the teachers have made important contributions to international Chinese language education and cultural communication. International Chinese language teachers are bound to face various discomfort and difficulties when working and living in a cultural environment different from their own, which affects both their teaching and personal life. This paper attempts to analyze the internal and external factors that affect the teachers’ cross-cultural adaptability, as well as the deficiencies in pre-job training for international Chinese language teachers, to find the existing problems and make suggestions to solve them.

Effect of Continuous Stress-annealing on the Structure and Magnetic Properties of Fe72.9Si15.8B6.9Nb3.2Cu1Co0.2 Amorphous Alloy

Wenfeng Zhang,Jianfeng Wang,Cheng Sun,Ting Zhang,Xueying Zhang,Tao Zhang 대한금속·재료학회 2023 METALS AND MATERIALS International Vol.29 No.10

The induced magnetic anisotropy, domain structure and magnetic properties of nanocrystallized Fe72.9Si15.8B6.9Nb3.2Cu1Co0.2 materials by continuous stress-annealing at 803–903 K under 0–75 MPa were systematically investigated. It was found that the tensile stress applied during annealing is beneficial to reduce the coercivity and core loss by refining the grain size of α-Fe phase. The remanence and permeability for the annealed samples at 853–903 K are negatively correlated with the induced anisotropy, which increases with the applied tensile stress. The stress-annealed samples yield magnetic easy plane perpendicular to the stress axis with an induced anisotropy constant greater than 1200 J/m3. The nanocrystalline cores with a low AC coercivity of 6.0 A/m and low core loss of 16.1 W/kg in combination with a high DC bias capability was successfully fabricated by stress-annealing the amorphous precursor at 878 K under 40 MPa. The current nanocrystalline material has potential for the application as anti-DC transformers in the electronic field.

Remimazolam Supplemented to General Anesthesia Alleviates Stress and Cognitive Impairment in Elder Patients After Hip Surgery

Yunyun Sun(Yunyun Sun),Jun Zhang(Jun Zhang),ShanWu Feng(ShanWu Feng) 대한신경정신의학회 2023 PSYCHIATRY INVESTIGATION Vol.20 No.5

Expression of the Arabidopsis vacuolar H+-pyrophosphatase gene AVP1 in peanut to improve drought and salt tolerance

Huaqin Sun,Qiang Gu,Sundaram Kuppu,Li Sun,Xunlu Zhu,Neelam Mishra,Rongbin Hu,Guoxin Shen,Junling Zhang,Yizheng Zhang,Longfu Zhu,Xianlong Zhang,Mark Burow,Paxton Payton,Hong Zhang 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3

The Arabidopsis gene AVP1 encodes an H?-pyrophosphatase that functions as a proton pump at thevacuolar membranes, generating a proton gradient acrossvacuolar membranes, which serves as the driving force formany secondary transporters on vacuolar membranes suchas Na?/H?-antiporters. Overexpression of AVP1 couldimprove drought tolerance and salt tolerance in transgenicplants, suggesting a possible way in improving drought andsalt tolerance in crops. The AVP1 was therefore introducedinto peanut by Agrobacterium-mediated transformation. Analysis of AVP1-expressing peanut indicated that AVP1-overexpression in peanut could improve both droughtand salt tolerance in greenhouse and growth chamberconditions, as AVP1-overexpressing peanuts producedmore biomass and maintained higher photosynthetic ratesunder both drought and salt conditions. In the field, AVP1-overexpressing peanuts also outperformed wild-type plantsby having higher photosynthetic rates and producing higheryields under low irrigation conditions.

Fabrication of Photopolymer Hierarchical Micronanostructures by Coupling Electrospinning and Photolithography for SERS Substrates

Wen-Yi Zhang,Xin-Ze Xiao,Chao Lv,Jia Zhao,Gong Wang,Xuan Gu,Ran Zhang,Bin-Bin Xu,Dan-Dan Zhang,Ai-Wu Li,Yong-Lai Zhang,Hong-Bo Sun 한국고분자학회 2013 Macromolecular Research Vol.21 No.3

Reported here is the fabrication of photopolymer hierarchical micronanostructures through a combinative process of electrospinning and subsequent photolithography. Electrospun SU-8 (epoxy-based negative photoresist)nanofiber films have been patterned into gratings with periods of 100, 200, 300, and 400 μm, respectively. Deposition of a silver nanolayer on these interlaced nanofiber films would lead to the formation of various plasmonic nanostructures,and therefore, giving rise to abundant surface-enhanced Raman scattering (SERS) “hot spots”. In the detection of Rhodamine 6G (R6G), probing molecule, the resultant SERS substrates show both high sensitivity and good reproducibility. The SERS enhancement factor could reach as high as ~108, indicating high efficiency. The fabrication of patterned, highly efficient SERS substrates may hold a great promise for the integration of SERS substrates in various microdevices such as microfluidic chips.

Studies on the Small Body Size Mouse Developed by Mutagen N-Ethyl-N-nitrosourea

Zhang, Qian-Kun,Cho, Kyu-Hyuk,Cho, Jae-Woo,Cha, Dal-Sun,Park, Han-Jin,Yoon, Seok-Joo,Zhang, ShouFa,Song, Chang-Woo Korean Society of ToxicologyKorea Environmental Mu 2008 Toxicological Research Vol. No.

Mutant mouse which show dwarfism has been developed by N-ethyl-N-nitrosourea (ENU) mutagenesis using BALB/c mice. The mutant mouse was inherited as autosomal recessive trait and named Small Body Size (SBS) mouse. The phenotype of SBS mouse was not apparent at birth, but it was possible to distinguish mutant phenotype from normal mice 1 week after birth. In this study, we examined body weight changes and bone mineral density (BMD), and we also carried out genetic linkage analysis to map the causative gene(s) of SBS mouse. Body weight changes were observed from birth to 14 weeks of age in both affected (n = 30) and normal mice (n = 24). BMD was examined in each five SBS and normal mice between 3 and 6 weeks of age, respectively. For the linkage analysis, we produced backcross progeny [(SBS${\times}$C57BL/6J) $F_1{\times}$ SBS] $N_2$ mice (n = 142), and seventy-four microsatellite markers were used for primary linkage analysis. Body weight of affected mice was consistently lower than that of the normal mice, and was 43.7% less than that of normal mice at 3 weeks of age (P < 0.001). As compared with normal mice at 3 and 6 weeks of age, BMD of the SBS mice was significantly low. The results showed 15.5% and 14.1 % lower in total body BMD, 15.3% and 8.7% lower in forearm BMD, and 29.7% and 20.1% lower in femur BMD, respectively. The causative gene was mapped on chromosome 10. The map order and the distance between markers were D10Mit248 - 2.1 cM - D10Mit51 - 4.2 cM - sbs - 0.7 cM - D10Mit283 - 1.4cM - D10Mit106 - 11.2cM - D10Mit170.

Improvement of ventricular remodeling and regulation of PI3K/Akt/mTOR signaling pathway in rats with heart failure by polysaccharides from Stropharia rugosoannulata

Zhang Gang,Sun Xiaofeng,Yu Kun,Zhang Xiwen,Yong Hui 대한독성 유전단백체 학회 2024 Molecular & cellular toxicology Vol.20 No.3

Background Heart failure is a syndrome of impaired cardiac circulation caused by the inability of the heart to provide adequate blood supply. Objective This study aimed to evaluate the improvement of ventricular remodeling and the regulation of PI3K/Akt/mTOR signaling pathway in rats with heart failure by polysaccharides from Stropharia rugosoannulata. Results In high dose group of polysaccharides from Stropharia rugosoannulata, the levels of LVESD, LVEDD, LVMI, CI, PVCA, CVF, and MMP-2 and MMP-9 mRNA expression in rats with heart failure were significantly decreased, while the levels of LVEF, LVFS were significantly elevated (P<0.05). In high dose group of polysaccharides from Stropharia rugosoannulata, the levels of PI3K, p-Akt/Akt, p-mTOR/mTOR, and cleaved-caspase 3 expression and TIMP-1 mRNA expression were significantly elevated, while Bax, p65, Bcl2 protein expression and serum levels of PRA, AngII, ALD, IL-6, TNF-α, ST2 and NT-proBNP were significantly decreased (P<0.05). Conclusions Polysaccharides from Stropharia rugosoannulata can improve ventricular remodeling and cardiac function, reduce inflammatory response and protect cardiac function in rats with heart failure in a dose-dependent manner, and the mechanism of action may be related to the regulation of MMPs/TIMPs balance, inhibition of RAAS system and activation of PI3K/Akt/mTOR signaling pathway. Background Heart failure is a syndrome of impaired cardiac circulation caused by the inability of the heart to provide adequate blood supply. Objective This study aimed to evaluate the improvement of ventricular remodeling and the regulation of PI3K/Akt/mTOR signaling pathway in rats with heart failure by polysaccharides from Stropharia rugosoannulata. Results In high dose group of polysaccharides from Stropharia rugosoannulata, the levels of LVESD, LVEDD, LVMI, CI, PVCA, CVF, and MMP-2 and MMP-9 mRNA expression in rats with heart failure were significantly decreased, while the levels of LVEF, LVFS were significantly elevated (P<0.05). In high dose group of polysaccharides from Stropharia rugosoannulata, the levels of PI3K, p-Akt/Akt, p-mTOR/mTOR, and cleaved-caspase 3 expression and TIMP-1 mRNA expression were significantly elevated, while Bax, p65, Bcl2 protein expression and serum levels of PRA, AngII, ALD, IL-6, TNF-α, ST2 and NT-proBNP were significantly decreased (P<0.05). Conclusions Polysaccharides from Stropharia rugosoannulata can improve ventricular remodeling and cardiac function, reduce inflammatory response and protect cardiac function in rats with heart failure in a dose-dependent manner, and the mechanism of action may be related to the regulation of MMPs/TIMPs balance, inhibition of RAAS system and activation of PI3K/Akt/mTOR signaling pathway.

Expression of Neurotrophin 4 and Its Receptor Tyrosine Kinase B in Reproductive Tissues during the Follicular and Luteal Phases in Cows

Sun, Yongfeng,Li, Chunjin,Sun, Yanling,Chen, Lu,Liu, Zhuo,Ma, Yonghe,Wang, Chunqiang,Zhang, Wei,Zhou, Xu Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.3

The neurotrophins, required for the survival and differentiation of the nervous system, are known to be important for the development of the reproductive tissues. However, the signals initiating the growth of follicles, gamete development, and transport and the development of zygote in the reproductive system of cows remain ambiguous. The purpose of the present study was to identify the transcripts and proteins of Neurotrophin 4 (NT4) and its receptor tyrosine kinase B (TrkB) in bovine reproductive tissues. The transcripts and immunoreactivity of NT4 and TrkB proteins were detected by reverse transcription polymerase chain reaction and western blot analysis. Using immunohistochemistry, the specific immunoreactivity of NT4 and TrkB were detected in the oocytes of primordial follicles and in the growing primary follicles. The NT4 and TrkB immunoreactivity was predominantly observed in granulosa cells, cumulus granulosa cells, cumulus oocyte complexes, theca cells of mature follicles, as well as in the oviduct epithelial cells, uterine gland cell, and epithelium cells of the uterus during the follicular and luteal phases in cows. Expressions of NT4 and TrkB mRNAs were not significantly different among the ovary, oviduct, and uterus of the follicular phase. For the luteal phase, the expression of NT4 mRNA in the ovary was significantly higher than that in the oviduct and uterus, and the expression of TrkB mRNA in the oviduct was significantly higher than that in the ovary and uterus, as determined by fluorescence quantitative reverse transcription polymerase chain reaction. The expression of NT4 mRNA was significantly higher than that of TrkB mRNA in the ovary and uterus, whereas NT4 mRNA expression was lower than that of TrkB mRNA in the oviduct during the luteal phase. The present study hypothesizes that NT4 participates in the regulation of both gonads and extra-gonadal reproductive tissues in cows.