Effect of Heat Treatment on the Lipophillic Pigments of Fresh Green Tea Liquor

Jian-Liang Lu,Zhan-Bo Dong,Shun-Shun Pan,Chen Lin,Xin-Qiang Zheng,Borthakur Devajit,Yue-Rong Liang 한국식품과학회 2009 Food Science and Biotechnology Vol.18 No.3

Changes in lipophillic pigments concentration and its relation to color of fresh green tea liquor during heat treatment were studied. The results showed liquor greenness decreased markedly with extension of incubation time at 55℃, while the brightness and yellowness changed a little. Significant increase in ‘a’ and ‘b’ values of tea liquor was observed at 95℃. Color change of liquor at 55℃ was accompanied by a decrease in the level of chlorophylls, lutein and neoxanthin, and an increase in the pheophytins and β-carotene levels. However, all pigments except β-carotene decreased with time extension at 95℃. Significant correlation was found between pigments and color difference index. The browning of fresh green tea liquor was attributed to vicissitudes of lipophillic pigments during heat treatment, especially to the change of chlorophylls/pheophytins ratio. Result also showed addition of Zn²? at 1.6 μ㏖/ℓ could partially alleviate the decrease in greenness during heat treatment.

Expression and regulation of Angiopoietins and their receptor Tie-2 in sika deer antler

Hong-Liang Zhang,Bin Guo,Zhan-Peng Yue,Lu Zhang,Zhan-Qing Yang,Shuang Geng,Kai Wang,Hai-Fan Yu 한국통합생물학회 2017 Animal cells and systems Vol.21 No.3

The cartilage vascularization and chondrocyte survival are essential for endochondral ossification which occurs in the process of antler growth. Angiopoietins (Ang) is a family of major angiogenic growth factors and involved in regulating the vascularization. However, the expression and regulation of Angs in the antler are still unknown. The aim of this study is to localize the expression of Ang-1, Ang-2 and their receptor Tie-2 in sika deer antler using in situ hybridization and focused on analyzing the regulation of testosterone, estrogen, all-trans-retinoic acid (ATRA) and 9cRA on their expression in antler chondrocytes. The results showed that Ang-1, Ang-2 and Tie-2 were highly expressed in antler chondrocytes. Administration of testosterone to antler chondrocytes led to a notable increase in the expression of Ang-1 and Tie-2, and a reduction in the expression of Ang-2. The similar result was also observed after estrogen treatment. In contrast, ATRA and 9cRA could inhibit the expression of Ang-1 in antler chondrocytes and heighten the expression of Ang-2. Simultaneously, ATRA could downregulate the expression of Tie-2 in antler chondrocytes at 12 and 24 h, while 9cRA upregulate the expression of Tie-2 at 3 and 6 h. Collectively, Ang-1, Ang-2 and Tie-2 are expressed in antler chondrocytes and their expression can be affected by testosterone, estrogen, ATRA and 9cRA.

Composition Dependence of Structural and Magnetic Properties of Mn<SUB>3+x</SUB>Ga<SUB>1-x</SUB>C (0 ≤ x ≤ 1)

Ying-Hua Liang,Ping-Zhan Si,Ting-Ting Qi,Xin-You Wang,Fei-Yang Wang,Qiong Wu,Hong-Liang Ge,Jihoon Park,Chul-Jin Choi 한국자기학회 2024 Journal of Magnetics Vol.29 No.1

Both Mn₄C (=Mn₃MnC) and Mn₃GaC have been studied previously. However, the reports on Mn<SUB>3+x</SUB>Ga<SUB>1-x</SUB>C (0 ≤ x ≤ 1) with intermediate compositions are very rare. In this work, the structure and magnetic properties of Mn<SUB>3+x</SUB>Ga<SUB>1-x</SUB>C prepared by using solid state reaction were studied systematically. High purity anti-perovskitetype Mn<SUB>3+x</SUB>Ga<SUB>1-x</SUB>C were obtained in the composition range of 0 ≤ x ≤ 0.5, above which Mn₂₃C₆ precipitates and the fraction of Mn₂₃C₆ in the samples increases with increasing x. The structural stability, lattice parameters, and room temperature saturation magnetization of ferromagnetic Mn<SUB>3+x</SUB>Ga<SUB>1-x</SUB>C (0 ≤ x ≤ 1) decreases with increasing x. The Curie temperature of Mn<SUB>3+x</SUB>Ga<SUB>1-x</SUB>C (0 ≤ x ≤ 1) increases with increasing x. Most Mn<SUB>3+x</SUB>Ga<SUB>1-x</SUB>C with varied x exhibit near-zero coercivity and zero remanent magnetization. This work indicates that the temperature coefficient of magnetization of Mn<SUB>3+x</SUB>Ga<SUB>1-x</SUB>C may be tuned by tuning the fraction of the Ga atoms.

Significant Differences in the Clinicopathological Characteristics and Survival of Gastric Cancer Patients from Two Cancer Centers in China and Korea

Zhan Long Shen,송교영,Ying Jiang Ye,Qi Wei Xie,Bin Liang,Kewei Jiang,박조현,Shan Wang 대한위암학회 2015 Journal of gastric cancer Vol.15 No.1

Purpose: To compare the clinicopathological data and long-term survival of gastric cancer patients in China and Korea. Materials and Methods: Patients who had undergone gastrectomy for gastric cancer between 1998 and 2009 in 2 high-volume institutions in both China (n=1,637) and Korea (n=2,231) were retrospectively evaluated. Clinicopathological variables, overall survival (OS), progression-free survival (PFS), and surgery-related complications were assessed for all patients and compared between the 2 institutions. Results: Chinese patients included in the study were significantly older and had a significantly lower body mass index (BMI) than the Korean patients. Esophagogastric junction tumors were more frequent in Chinese patients. However, the number of patients with stage I gastric cancer, the number of harvested lymph nodes, and the number of total gastrectomies were significantly higher in the Korean population. Korean patients also presented with fewer undifferentiated tumors than Chinese patients. Furthermore, Korean patients had prolonged OS and PFS for stage III cancers only. BMI, tumor-node-metastasis (TNM) stage, tumor invasion, number of positive lymph nodes, and distant metastases were all independent factors affecting OS and PFS. Conclusions: Although China and Korea are neighboring Asian countries, the clinicopathological characteristics of Chinese patients are significantly different from those of Korean patients. Korean gastric cancer patients had longer OS and PFS than Chinese patients. Influencing factors included TNM stage, tumor invasion, and lymph node metastasis.

Overexpression of a Lotus corniculatus AP2/ERF transcription factor gene, LcERF080, enhances tolerance to salt stress in transgenic Arabidopsis

Zhan-Min Sun,Yan-Min Wu,Mei-Liang Zhou,Xing-Guo Xiao,Yi-Xiong Tang 한국식물생명공학회 2014 Plant biotechnology reports Vol.8 No.4

The APETALA2/ethylene-responsive elementbinding factors (AP2/ERF) play central roles in the stressresponse in plants. In this study, we identified and isolateda novel salt stress-related gene, LcERF080, that encodes anAP2/ERF protein in Lotus corniculatus cultivar Leo. LcERF080 was classified into the B-4 group of the ERFsubfamily based on multiple sequence alignment andphylogenetic characterization. Expression of LcERF080was strongly induced by salt, abscisic acid, 1-aminocyclopropane-1-carboxylic acid, methyl jasmonate, and salicylicacid stresses. Subcellular localization assay confirmedthat LcERF080 is a nuclear protein. LcERF080 overexpressionin Arabidopsis resulted in pleiotropic phenotypeswith a higher seed germination rate and transgenic plantswith enhanced tolerance to salt stress. Further, under stressconditions, the transgenic lines exhibited elevated levels ofsoluble sugars and proline as well as relative moisturecontents but a lower malondialdehyde content than incontrol plants. The expression levels of hyperosmoticsalinity response genes COR15A, RD22, and P5CS1 werefound to be elevated in the LcERF080-overexpressingArabidopsis plants compared to the wild-type plants. Theseresults reveal that LcERF080 is involved in the responsesof plants to salt stress.

Network Isolation in Electrical Vehicle Charging Station

Zhan Xiong,Liu Jian,Liang Xiao,Zhao Ting,Tian Wei,Rao Xue,Ru Yeqi 보안공학연구지원센터 2016 International Journal of Future Generation Communi Vol.9 No.5

Network isolation system (NIS) can help electrical vehicle charging station (EVCS) to exchange messages with different systems (such as central monitor system, battery package and electrical vehicle (EV)) in a secure and efficient way. This paper focuses on the structure of EVCS, and then analyzes the boundaries of the trusted and the untrusted areas. On the top boundary, EVCS can communicate with the central monitor system. On bottom boundary, EVCS can communicate with EVs or battery packages. Based on this demarcation, this paper proposes the NIS architecture for EVCS. The new architecture can limit the top/bottom boundary of EVCS to enhance the securities

Muscle-derived Stem Cells Differentiate into Functional Smooth Muscle Cells for Ureter Tissue Engineering: An Experimental Study

Zhan-Kui Zhao,Hong-Lian Yu,Fei Xiao,Shi-Wen Li,Wen-Biao Liao,Kai-Liang Zhao 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.3

We assessed the ability of muscle-derived stem cells (MDSC) to differentiate into smooth muscle cells (SMC) and their potential to promote the regeneration of smooth muscle with a vessel extracellular matrix (VECM)for tissue engineering of the ureter. MDSC were isolated,proliferated, and identified by flow cytometry. SMC phenotype differentiation was induced with a smooth muscle induction medium. Gene expression was evaluated by real-time quantitative polymerase chain reaction (PCR)and Western blot studies. The VECM was obtained by a decellularization process, and cytotoxic effects were evaluated by exposing the induced cells to a VECM extract. The induced cells were seeded onto VECM in vitro for 1 week, and then the compound grafts were used for ureter reconstitution in vivo. The grafts were obtained for histological studies at 2, 4, 8, and 16 weeks post-operation. Intravenous urography was used to evaluate renal function and ureteral patency. Flow cytometry demonstrated that the MDSC expressed Sca-1 and desmin, but did not express CD45. After induction, SMC phenotype gene expression was confirmed in the induced cells by real-time quantitative PCR and Western blot studies. VECM exhibited a nontoxic effect on the induced cells in vitro. At 16 weeks postoperation,a histological evaluation showed that multilayered urothelium and organized muscle fiber bundles had formed in the grafts. Intravenous urography demonstrated no evidence of ureteral stricture or hydroureteronephrosis. These results demonstrate that MDSC can be induced into SMC and that this was useful for promoting regeneration of smooth muscles for ureter tissue engineering. We assessed the ability of muscle-derived stem cells (MDSC) to differentiate into smooth muscle cells (SMC) and their potential to promote the regeneration of smooth muscle with a vessel extracellular matrix (VECM)for tissue engineering of the ureter. MDSC were isolated,proliferated, and identified by flow cytometry. SMC phenotype differentiation was induced with a smooth muscle induction medium. Gene expression was evaluated by real-time quantitative polymerase chain reaction (PCR)and Western blot studies. The VECM was obtained by a decellularization process, and cytotoxic effects were evaluated by exposing the induced cells to a VECM extract. The induced cells were seeded onto VECM in vitro for 1 week, and then the compound grafts were used for ureter reconstitution in vivo. The grafts were obtained for histological studies at 2, 4, 8, and 16 weeks post-operation. Intravenous urography was used to evaluate renal function and ureteral patency. Flow cytometry demonstrated that the MDSC expressed Sca-1 and desmin, but did not express CD45. After induction, SMC phenotype gene expression was confirmed in the induced cells by real-time quantitative PCR and Western blot studies. VECM exhibited a nontoxic effect on the induced cells in vitro. At 16 weeks postoperation,a histological evaluation showed that multilayered urothelium and organized muscle fiber bundles had formed in the grafts. Intravenous urography demonstrated no evidence of ureteral stricture or hydroureteronephrosis. These results demonstrate that MDSC can be induced into SMC and that this was useful for promoting regeneration of smooth muscles for ureter tissue engineering.

Examining the evolving structures of intercity knowledge networks: the case of scientific collaboration in China

Dai Liang,Derudder Ben,Cao Zhan,Ji Yufan 서울시립대학교 도시과학연구원 2023 도시과학국제저널 Vol.27 No.3

Drawing on data on scientific co-publications derived from the Web of Science for the periods 2002–2006 and 2012–2016, we construct and analyse a key element of China's intercity knowledge networks (CIKNs): scientific collaboration networks. Employing network-analytical and exponential random graph modelling techniques, we examine the evolving structures and driving mechanisms underlying these CIKNs. Our results show that the density of the CIKNs has significantly increased over time. CIKN flows are dense in the Southeastern but sparse in the Northwestern part of China, with the Hu Line acting as a clearly visible border. As the dominant knowledge centre, Beijing is involved in scientific collaboration networks throughout the country, with the diamond-shaped structure anchored by Beijing-Shanghai-Guangzhou-Chengdu becoming evident. We find that preferential attachment and transitivity are significant endogenous processes driving scientific collaboration, while a city's administrative level and R&D investment are the strongest exogenous factors. The impact of GDP and geographical proximity is limited, with institutional proximity being the most sizable of the well-known suite of proximity effects.

Damage detection of shear buildings through structural mass-stiffness distribution

Yabin Liang,Dongsheng Li,Gangbing Song,Chao Zhan 국제구조공학회 2017 Smart Structures and Systems, An International Jou Vol.19 No.1

For structural damage detection of shear buildings, this paper proposes a new concept using structural element mass-stiffness vector (SEMV) based on special mass and stiffness distribution characteristics. A corresponding damage identification method is developed combining the SEMV with the cross-model cross-mode (CMCM) model updating algorithm. For a shear building, a model is assumed at the beginning based on the building\'s distribution characteristics. The model is updated into two models corresponding to the healthy and damaged conditions, respectively, using the CMCM method according to the modal parameters of actual structure identified from the measured acceleration signals. Subsequently, the structural SEMV for each condition can be calculated from the updated model using the corresponding stiffness and mass correction factors, and then is utilized to form a new feature vector in which each element is calculated by dividing one element of SEMV in health condition by the corresponding element of SEMV in damage condition. Thus this vector can be viewed as a damage detection feature for its ability to identify the mass or stiffness variation between the healthy and damaged conditions. Finally, a numerical simulation and the laboratory experimental data from a test-bed structure at the Los Alamos National Laboratory were analyzed to verify the effectiveness and reliability of the proposed method. Both simulated and experimental results show that the proposed approach is able to detect the presence of structural mass and stiffness variation and to quantify the level of such changes.

Bioinspired Fabric with Superhydrophilicity and Superoleophobicity for Efficient Oil/Water Separation

Liping Liang,Chenlu Wang,Hongfang Wang,Haihua Zhan,Xu Meng 한국섬유공학회 2018 Fibers and polymers Vol.19 No.9

The cotton fabric was modified with dopamine methacrylamide (DMA) based on mussel-inspired reaction and polymerized with zwitterionic sulfobetaine methacrylate (SBMA) through free radical polymerization reaction. The poly(DMA-SBMA) contained not only key chemical constituents of dopamine hydrochloride, which strongly adsorbed to fabric substrates, but also hydrophilic groups, providing a hydrophilic surface for fabric due to its strong interaction with water via electrostatic interactions. The chemical structure, surface topography, and surface wettability of the fabric were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and contact angle experiments, respectively. The results showed that the water contact angle (WCA) of the treated fabric was ~0 o, whereas the underwater oil contact angle (OCA) was ~161 o, as compared to ~25 o for the control one. It is expected that as-prepared fabrics could be applied in oil/water separation due to such special superhydrophilicity and superoleophobicity.