Finding Blue Tracks in Gephyrocharax melanocheir Fish Similar to the Locations of Acupuncture Meridians after Injecting Alcian Blue

Ze Wang,Wei-Bo Zhang,Shuyong Jia,Yu-Ying Tian,Guang-Jun Wang,Hongyan Lin 사단법인약침학회 2015 Journal of Acupuncture & Meridian Studies Vol.8 No.6

This study investigated whether a meridian-like distribution of Alcian blue (AB) existed after it was injected into a fish's body and suggested a new animal model for meridian study. Twenty Gephyrocharax melanocheir fish with translucent bodies were injected with AB at a point near the spinal column or the dorsal fin. Distribution of AB was observed using a digital camera and a stereomicroscope. Three or more obvious blue tracks were found: one along the spinal column, another along the posterior margin of the abdomen extending to the superior margin of the anal fin, and a third along both sides of the dorsal fin. They were similar to the locations of the governor, conceptual vessel, and urinary bladder meridians, respectively, on the human body according to the classic theory of traditional Chinese medicine. A few other blue tracks were also found, which apparently did not correspond to any known meridians. The results show that the tracks of AB share important similarities with the locations of classically described meridians and that they are mainly distributed in the interstitial space around bones and blood vessels and inside muscular interstices. This study may provide a new experimental animal model for exploring acupuncture meridians.

Molecular identification of DNA barcoding of Leguminous toxic species and quantitative analysis by ELISA kits

Wang Jie,Wang Shuangyu,Sun Fenglin,Liu Chang,Zhao Jinquan,Yu Hongwei,Lv Xiaojing,Liu Ze,Bu Shuhua,Yu Weisen 한국식물생명공학회 2024 Plant biotechnology reports Vol.18 No.2

Some edible Leguminous are toxic when raw, and the Chinese are particularly fond of beans, so Leguminous poisoning is very common in China. Rapid and accurate identification of poisoned species and determination of their toxic components would better assist physicians in treating patients. However, traditional morphology-based identification methods possess many limitations. DNA barcoding technique is a new species identification technique developed in recent years, which is expected to make up for the shortcomings of traditional morphological identification. In this study, a comprehensive evaluation system based on DNA barcoding and ELISA kits was attempted. A total of 30 Leguminous toxic plants were collected, involving 9 genera and 10 species. We used simulated gastric fluid (SGF) to simulate the human gastric environment. Three markers (rbcL, trnH-psbA, and ITS) were amplified and sequenced for all untreated and 15 mock-digested samples. The validity of DNA barcoding for species identification was assessed using the Basic Local Alignment Search Tool (BLAST) method and the tree construction method. The levels of three toxic components (saponin, phytoagglutin and trasylol) were determined in all samples using ELISA kits. The amplification success rate of all three regions was high (rbcL 96.67%, trnH-psbA 100%, and ITS 100%), but the sequencing of the trnH-psbA region was less satisfactory (66.67%), and SGF had a significant impact on the sequencing of the ITS region (After 40 min of SGF treatment, the sequencing success rate decreased by 46.67%). The samples from different species and origins contained different levels of toxic components, and the levels of all three sub- stances decreased significantly after undergoing SGF digestion. After 1 h of SGF treatment, the saponin content decreased to 0–8.60% in untreated content (PHA decreased to 8.62–36.88%, trasylol decreased to 4.70–47.06%). The current results suggest that DNA barcoding has great potential for rapid identification of Leguminous poisoning in clinical settings. Toxins are probably not detectable in the patient for longer periods of poisoning. We recommend DNA barcoding technology as a first step for rapid screening and combined with toxin analysis for clinical diagnosis.

시선추적기법을 활용한 노인의 주거환경 내 색 인지에 대한 연구

왕택우(Wang, Ze-Yu),조지영(Cho, Ji Young) 한국주거학회 2022 한국주거학회 학술대회논문집 Vol.33 No.2

As people get old, people experience the decline of physiological functions which may increase the incidence of dementia and cognitive impairment. This will greatly affect the care burden of the elderly themselves and their family members. At the same time, in severe cases, there will be problems such as going out alone and getting lost. The purpose of this study is to understand the gaze of the elderly on the elements in the space through the application of eye tracking technology, especially through the change of color, to explore the degree of attention to the door. This study conducted experiments on 9 elderly people. The experimental stimuli were two groups of pictures with different colors in entry doors, with 7 pictures in each group. The results from this eye tracking research shows that: (1) The application of color can effectively change the attention of the elderly, and the probability of being observed is higher in red doors with contrasting white walls than white doors in white walls; (2) Red doors are noticed faster than white doors; and (3) Compared with normal elderly, people with cognitive impairment showed the attention to the images were not obvious, and their eye movement were more spread than those with healthy. The results from this research highlights the necessity to plan colors of entry doors carefully depending on the intention in expecting more usage or less usage for their safety and protection from way loss.

동시출현단어 분석을 이용한 노인주거디자인 국내외 연구동향 비교 - Web of Science Core Collection과 KCI-Korean 저널 데이터베이스를 중심으로 -

왕택우(Wang, Ze-Yu),조지영(Cho, Ji Young),홍이경(Hong, Yi-Kyung) 한국주거학회 2021 한국주거학회 논문집 Vol.32 No.6

시선추적기법을 활용한 노인의 주거환경 내 색 인지에 대한 연구

왕택우(Wang, Ze-Yu),조지영(Cho, Ji Young) 한국주거학회 2022 한국주거학회 학술대회논문집 Vol.34 No.1

As people get old, people experience the decline of physiological functions which may increase the incidence of dementia and cognitive impairment. This will greatly affect the care burden of the elderly themselves and their family members. At the same time, in severe cases, there will be problems such as going out alone and getting lost. The purpose of this study is to understand the gaze of the elderly on the elements in the space through the application of eye tracking technology, especially through the change of color, to explore the degree of attention to the door. This study conducted experiments on 9 elderly people. The experimental stimuli were two groups of pictures with different colors in entry doors, with 7 pictures in each group. The results from this eye tracking research shows that: (1) The application of color can effectively change the attention of the elderly, and the probability of being observed is higher in red doors with contrasting white walls than white doors in white walls; (2) Red doors are noticed faster than white doors; and (3) Compared with normal elderly, people with cognitive impairment showed the attention to the images were not obvious, and their eye movement were more spread than those with healthy. The results from this research highlights the necessity to plan colors of entry doors carefully depending on the intention in expecting more usage or less usage for their safety and protection from way loss.

Comparison of Liver Transplantation and Liver Resection for Hepatocellular Carcinoma Patients with Portal Vein Tumor Thrombus Type I and Type II

Jia-Yu Lv,Ning-Ning Zhang,Ya-Wei Du,Ying Wu,Tian-Qiang Song,Ya-Min Zhang,Yan Qu,Yu-Xin Liu,Jie Gu,Ze-Yu Wang,Yi-Bo Qiu,Bing Yang,Da-Zhi Tian,Qing-Jun Guo,Li Zhang,Ji-San Sun,Yan Xie,Zheng-Lu Wang,Xin 연세대학교의과대학 2021 Yonsei medical journal Vol.62 No.1

Purpose: The aim of this study was to compare the efficacy of liver transplantation (LT) and liver resection (LR) for hepatocellularcarcinoma (HCC) patients with portal vein tumor thrombus (PVTT) and to investigate risk factors affecting prognosis. Materials and Methods: A total of 94 HCC patients with PVTT type I (segmental PVTT) and PVTT type II (lobar PVTT) were involvedand divided into LR (n=47) and LT groups (n=47). Recurrence-free survival (RFS) and overall survival (OS) were comparedbefore and after inverse probability of treatment weighting (IPTW). Prognostic factors for RFS and OS were explored. Results: Two treatment groups were well-balanced using IPTW. In the entire cohort, LT provided a better prognosis than LR. Among patients with PVTT type I, RFS was better with LT (p=0.039); OS was not different significantly between LT and LR(p=0.093). In subgroup analysis of PVTT type I patients with α-fetoprotein (AFP) levels >200 ng/mL, LT elicited significantly longermedian RFS (18.0 months vs. 2.1 months, p=0.022) and relatively longer median OS time (23.6 months vs. 9.8 months, p=0.065). Among patients with PVTT type II, no significant differences in RFS and OS were found between LT and LR (p=0.115 and 0.335,respectively). Multivariate analyses showed treatment allocation (LR), tumor size (>5 cm), AFP and aspartate aminotransferase(AST) levels to be risk factors of RFS and treatment allocation (LR), AFP and AST as risk factors for OS. Conclusion: LT appeared to afford a better prognosis for HCC with PVTT type I than LR, especially in patients with AFP levels>200 ng/mL.

집중력과 창의성에 관련된 실내환경 특성에 관한 EEG 연구

왕택우(Wang, Ze-Yu),조지영(Cho, Ji Young),홍이경(Hong, Yi-Kyung) 한국주거학회 2023 한국주거학회 학술대회논문집 Vol.35 No.2

한중 노인주거디자인 연구의 경향 비교 분석

왕택우(Wang, Ze-Yu),조지영(Cho, Ji Young),홍이경(Hong, Yi-Kyung) 한국주거학회 2021 한국주거학회 학술대회논문집 Vol.33 No.1

In aging society, providing comfortable and optimized residential environment for the elderly is one of the essential tasks for researchers. This is a comparative study of research trends of Elderly Residential Design in Korea and China using co-occurrence analysis method. By comparing themes of existing studies conducted in both countries and looking at the areas that are active and those that are not, this study aims to present the direction of future research on residential design for the elderly in both countries. We searched for Elderly Residential Design as a keyword on academic sites in both countries (KISS in Korea and CNKI in China) to obtain research data, and then input them into VOSviewer, a software tool for constructing and visualizing bibliometric networks. The results show that existing studies on Elderly Residential Design in Korea were mainly about semi-self-care elderly living, elderly housing theory method, elderly housing design, and elderly physiology and psychology. In contrast in China, development policy, residential design project, residential design for the elderly, and housing theory for the elderly were the main directions. It is hoped that the findings from this study will provide implications for the research direction of the residential design for the elderly in both countries.

Application of N-substituted (aminomethyl)benzoate Strategy in Design of Scutellarein Derivatives with Improved Caco-2 Cell Permeability and In Vitro Antioxidative Activity

Ze-Qin Dai,Hang Su,Min Luo,Yu Ou,Xiao-Zhong Fu,Yong-Xi Dong,Yu-Feng Cha,Shun Zhang,Yong Huang,Yong-Lin Wang 대한화학회 2015 Bulletin of the Korean Chemical Society Vol.36 No.8

A series of 4′-N-substituted (aminomethyl)benzoate derivatives of scutellarein were designed and synthesized. Evaluation of their physiochemical properties showed that the designed target compounds 5a–e exhibit higher chemical stability and aqueous solubility than scutellarin and scutellarein. The permeability (Papp AP to BL ) of 5c–e in Caco-2 cells were 2.8, 8.1, and 12.6 times higher than that of scutellarin and 1.3, 4.1, and 6.0 times higher than that of scutellarein; especially, 5e had the highest P app AP to BL value (7.19 ± 0.31 × 10−6 cm/s) and the lowest ER (P app BL to AP /P app AP to BL ) value of 0.17. In vitro antioxidative evaluation results revealed that 5e could protect against H2O2 -induced PC12 cells’ oxidative damage by attenuating mitochondrial membrane potential loss and decreasing H2O2 -induced reactive oxygen species (ROS) production.

Identification of interacting proteins of retinoid-related orphan nuclear receptor gamma in HepG2 cells

( Ze Min Huang ),( Jun Wu ),( Zheng Cai Jia ),( Yi Tian ),( Jun Tang ),( Yan Tang ),( Ying Wang ),( Yu Zhang Wu ),( Bing Ni ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2012 BMB Reports Vol.45 No.6

The retinoid-related orphan nuclear receptor gamma (RORγ) plays critical roles in regulation of development, immunity and metabolism. As transcription factor usually forms a protein complex to function, thus capturing and dissecting of the RORγ protein complex will be helpful for exploring the mechanisms underlying those functions. After construction of the recombinant tandem affinity purification (TAP) plasmid, pMSCVpuro RORγ-CTAP(SG), the nuclear localization of RORγ-CTAP(SG) fusion protein was verified. Following isolation of RORγ protein complex by TAP strategy, seven candidate interacting proteins were identified. Finally, the heat shock protein 90 (HSP90) and receptor-interacting protein 140 (RIP140) were confirmed to interplay with RORγ by co-immunoprecipitation. Interference of HSP90 or/and RIP140 genes resulted in dramatically decreased expression of CYP2C8 gene, the RORγ target gene. Data from this study demonstrate that HSP90 and RIP140 proteins interact with RORγ protein in a complex format and function as co-activators in the RORγ-mediated regulatory processes of HepG2 cells. [BMB Reports 2012; 45(6): 331-336]